The newly developed varieties, Jayoung (violet flesh color) and Hongyoung (red flesh color) that harboring various anthocyanins and flavonoids in flesh colored potato are highly increase their interesting not only for food but also functional characteristics such as anti-inflammatory effects. Up to date, most of the molecular markers developed in potato are linked to disease resistance including late blight and PVY, nematode. A few markers linked to economically important functional materials such as anthocyanin biosynthesis are published. With the low cost and high throughput of NGS (Next Generation Sequencing) technology, numerous molecular markers are highly increased in may crops. Among the molecular markers, SNPs (Single nucleotide Polymorphisms) are most useful markers owing to their large numbers in inter and intra varieties in potato. Here we reported SNPs discovery from transcriptome sequencing data acquired from colored flesh potato cultivars, Jayoung and Hongyoung with white flesh color Atlantic. Total RNA was isolated from shoot in tuber after breaking dormancy about 2cm length. Short read sequence data were obtained form Illumina Hiseq2000 and the raw dat set were trimmed with Q socore over 20. Sequencing data were align to reference genome (Solanum tuberosum v4.03, http://potatogeomics.plantbiolgy.msu.edu). About 70% of sequence read were mapped int to reference genome. 139,050, 140,976 and 146,429 total SNPs were discovered in Hongyoung, Jayoung and Atlantic, respectively. All SNPs are mapped into the psedomolecules in reference genome by chromosome. SNPs are also analyzed with homozygous and heterozygous SNPs and genic and intergenic region. SNPs are compared with Potato Infinium 8K Chip data. SNPs found in candidate genes of anthocyanin biosynthesis were discovered. These SNPs information of flesh colored potato will be further analyzed for the allele mining for anthocyanin syhthesis and control region

‘고운’은 농촌진흥청 국립식량과학원 고령지농업연구센터에서 가을재배용 칩가공용 품종을 육성하고자 1998년 건물함량이 높은 가공용품종 ‘Lemhi russet’와 수량성이 높은 단휴면 2기작 식용품종인 ‘추백’을 인공교배하여 육성하였다. 그후 봄재배와 가을재배를 통하여 실생세대와 생산력검정예비시험, 생산력검정본시험을 거치면서 H98005-4를 선발하였으며, 2005년과 2006년에 걸쳐 제주, 남해, 강릉 등 3지역에서봄, 가을재배를 통해 지역적응시험을 실시한 결과 그 우수성이 인정되어 2006년 농작물 직무육성 신품종 선정위원회에서국가목록 등재품종으로 선정되었다. ‘고운’은 남부 해안 2기작감자 재배지역에 적응하는 칩가공용 감자품종으로 숙기는중간정도이다. 반직립형으로 생장하며, 꽃색이 흰색이고, 자연조건하에서 열매는 거의 달리지 않는다. 괴경모양이 짧은 계란형으로 육색이 흰색이며, 내외부 생리장해 발생이 적다. 괴경의 건물함량이 높고 환원당 함량이 낮아 칩가공성이 우수하다. 괴경의 휴면기간은 수확후 60～70일 정도이다. 더뎅이병과 역병에 대하여 중도저항성이지만 감자바이러스Y에는 약하기 때문에 씨감자 채종재배시 진딧물 방제를 철저히 하여야한다. 풋마름병에 대해서는 저항성이 없으므로 풋마름병 발생지역에서는 재배하지 말아야 한다. ‘고운’의 수량성은 2005년부터 2006년까지 2년간 실시한 지역적응시험 결과 봄재배시33.2 ton/ha, 가을재배시 26.2 ton/ha로 나타나 ‘Dejima’에 비하여 각각 87%, 86%의 수량성을 보였다. ‘고운’의 적응지역은 전라북도 해안지역과 전라남도와 경상남도의 2기작감자 재배지역이다.

준고랭지에서는 여름감자를 수확하기 전에 배추, 무, 쥬키니호박 등의 작물을 감자 이랑 위에 바로 심고 후작물을수확한 후 10월 하순이나 11월 상순에 감자를 캐는 작형으로 농사를 짓는다. 이 때 전작물인 감자와 후작물의 품질 및수량성을 비교, 검토하여 농가에서 최적 재배법을 이용할수 있도록 하기 위하여 본 시험을 수행하였다. 품종은 중생종인 ‘수미’ 품종과 만생종인 ‘하령’ 품종을 사용하였으며 후작물처리는 무처리, 무, 배추구를 두고 품종별 난괴법 3반복을 처리하였으며 감자는 흑색비닐멀칭을 하여 재배하였다. 감자 수량조사는 적기수확, 15일, 30일 45일, 60일에 하였으며 후작물은 수확기에 수량조사를 실시하였다. 그 결과, 품종은 만생인 하령품종보다 중생종인 수미품종이 내부생리장해가 적고 상품수량이 높아 전작물 품종으로 적합하였으며 수미품종의 경우 무처리보다는 무, 배추 정식구에서부패서율, 기형서율, 녹화율이 낮게 나타났으며 상품서율과상품수량은 후기까지 유지되었다. 이상의 결과를 종합해볼때 준고랭지에서 8월 중하순에서 9월 상순에 감자를 수확하지 않고 후작물(무, 배추)를 재배한 후 감자를 수확하여도수량성에는 문제가 없는 것으로 나타났으며 후작물의 수량성 또한 높게 나타났다.

The high temperature due to climate change may result in the intensification of several drought and heat stress on crops including potato. These abiotic stress affect on potato development staages; sprout development, tuber initiation and maturation. Potatoes need moderate amounts of nitrogen and cool night for good tuber growth. Especially, high temperature in soil will delay tuber initiation and induce malformation. Therefore, to identify quickly heat tolerant lines and breeding potato lines adapt to high temperature in the field are needed. The objectives of this study were as follows; To apply in vitro screening method for identifying potato lines adapted to high temperature conditions. To verify these results under field assays carried out under natural high temperature field conditions. We used in vitro screening methods with breeding lines from Intranational Potato Center(CIP) under three temperature regime, 18℃, 25℃ and 30℃. All breeding liens had some genotype that produced microtubers at 18℃ and 25℃, with a clear tendency for lower percentage of tuberization at the high temperature. To verify in vitro screening methods for heat tolerance lines, we carried out natural high temperature filed evaluation at Tacna, La Molina and Sanramon in Peru. The results of both the in vitro test and the field assay showed clear relationship and similar expression of tuberization percentage. This finding supports the use of the in vitro assay as a rapid screening methods that represents performance at the field level. But the correlation between performance of the breeding lines under the in vitro and field condions was low. This could be due to differential response to breeding lines to characteristics of the field environment, such as soil salinity, drought, which were not represented in the in vitro assay.

The experiment was conducted to investigate the potential for environmentally friendly cultivation of cv. Haryeong, which was bred by the National Institute of Highland Agriculture in 2006, through evaluation of the late blight field resistance at the farmer's practices level. cv. Haryeong showed higher field resistance to late blight as 590.3 in mean Area Under the Disease Progress Curve (AUDPC) during the cultivation period than cv. Superior as 1377.7 in AUDPC. However there was no observed different trend among the experimental treatments. Mean of tuber yield of cvs. Haryeong and Superior were 2,870 kg/10a and 2,301 kg/10a, respectively, and in all experimental treatments Haryeong was 25% higher than those of Superior. Mean of dry matter content of Haryeong and Superior were 18.1% and 16.1%, respectively, and there were no significant differences among the experimental treatments. cv. Haryeong was confirmed as a suitable cultivar for environmentally friendly cultivation in this experiment. In the future, additional research on fertilization and disease management will be required.