Acteoside acts as an anti-oxidative activity and anti-apoptosis in the cells. But, it has been not studied on maturation and development of porcine oocytes. The aims of the present study were to examine the effects of acteoside on the morphological progress of meiosis, developmental competence, and ROS in porcine oocytes. Oocytes were matured in tissue culture medium-199, supplemented with acteoside at various concentrations: 0 (control), 10, 30 and 50 μM. The oocytes maturation rates of groups supplemented with acteoside were no significantly different (81.13, 85.96, 82.95 and 83.68%, respectively). Level of ROS was significantly decreased in acteoside treated group. Furthermore, the parthenogenetic blastocyst rate was significantly improved in 10 μM acteoside treated group compared with control group (44.83 vs. 27.75%). And we investigated effect of acteoside on the oocytes condition represented by cytoplasmic maturation by homogeneous distribution and formation of cytoplasmic organelles and regulation of apoptosis-related genes. In the results. during IVM, 10 μM acteoside treated oocytes showed that the mitochondria and lipid droplet were smaller and homogeneous distribution in cytoplasm compare with control oocytes. And reverse transcription polymerase chain reaction (RTPCR) of parthenogenetic blstocysts revealed that acteoside increased the anti-apoptotic genes (Mcl-1, Bcl-2 and Bcl-xL), whereas reduced the expression of pro-apoptotic genes (Bax and Bak). In conclusion, based on the results, the effect of acteoside on IVM was not attractive. However, in acteoside treated group, cytoplasmic maturation seemed to be improved with morphologically uniform distribution of cytoplasmic organelles. Furthermore, embryonic development in acteoside treated group was significantly highly increased than that of non-treated group. Our results represents that addition of acteoside to the IVM medium has a beneficial effect in physiology of porcine oocytes, providing a improved method for porcine oocytes in vitro. * This work was supported by a grant (Code# PJ008148) from BioGreen21 Program, Rural Development Administration, Republic of Korea.

Field research was undertaken for recovering mosquito larval mermithid parasite, Romanomermis species in rice fields in 54 different areas in period of May through October, 2009. Of 54 area rice fields surveyed, Romanomermis sp. recoveries were made from 4 areas. 32 Anopheles mosquito larvae, malaria disease vector were infected and died from samples collected in Pochon area, and a total of 10 Culex mosquito larvae, house frequenting mosquito were infected to death in 3 different areas, Gimpo, Sangju and Gurae, respectively. On the basis of positive natural infection record, an intensive host-parasite occurrences and/or population study was surveyed in foci area of Pochon in small pond (P) and Rice paddies (A and B) during 5 months till end of October. The natural mermithid infection was continuously occurred from June through October in weekly samples, however the rate of infection was appeared higher in June-July, thereafter the rate gradually decreased in progress of the season. The highest natural infection rate was observed from the Pond 9.1% followed by Rice paddy "A" 5.2%, Rice paddy "B" 2.4%, respectively. Including 2 other Rice paddies "C" and "D", a total mosquito larvae collected was 3,270, an overall average natural infection rate was recorded as 3.7% (121 Anopheles mosquito larvae infected).

14-3-3 proteins are known to play a pivotal role in a diverse array of cellular events such as cell survival, apoptosis, and signal transduction. Numerous 14-3-3 ζ have been cloned and characterized from a host of eukaryotic organisms including human, plants, yeast, fruit fly and silkworm. However, no study on Spodoptera exigua 14-3-3ζ in conjunction with virus infection has so far been reported in insects. It appears that expression of Se14-3-3ζ was decreased starting 24 h post-SeNPV infection as SeNPV titers seemed to increase as evidenced by intense bands of SeNPV IAP3. Interestingly, confocal microscopic analysis revealed that Se14-3-3ζ is expressed at the apical side of the NPV-uninfected gut cells, whereas it was detected mainly in the nucleus of the NPV-infected cells. Thus, despite the biological significance of Se14-3-3ζ in S. exigua in conjunction with molecular interactions between SeNPV and S. exigua is unclear now, our data suggest that Se14-3-3 ζ protein plays a role to protect S. exigua from the infection or inhibit replication of SeNPV.

Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway is known to play a pivotal role in various cellular events and antiviral responses in both vertebrates and insects. In an attempt to elucidate the potential involvement of STAT on S. exigua-SeNPV interactions, the full length cDNA of SeSTAT was cloned from S. exigua. Analysis of temporal expression patterns shows that SeSTAT is expressed in all stages of life cycle such as larvae, pupae, and adult. Spatial expression analysis shows that it is highly expressed in fat body and Malpighian tubule. Interestingly, SeSTAT is induced at 24 h in response to either laminarin or LTA injection in larvae. Electrophoretic mobility shift assay (EMSA) shows that the binding of nuclear extracts from fat body cells immune-challenged with LTA to STAT5 probe was observed. In addition, SeSTAT was nuclear-translocalized in both fat body and gut cells that were challenged with LTA and laminarin, respectively. Finally, gene silencing of SeSTAT shows that SeNPV number appears to be increased. It suggests that SeSTAT may act as a negative regulator against SeNPV in midgut.

This study was carried out to investigate chemical ingredient of Amillaria tabescens. Methanol extract of Amillaria tabescens was analyzed by column chromatography, thin layer chromatography, HPLC, mass spectrophotometer. ATB201 was isolated by prep-HPLC from butyl alcohol fraction. From the physico-chemical data, including NMR, MS, the chemical structure of the compound was determined to be an uracil. (Fig. 1). The molecular weights of uracil was 112.

This study was carried out to investigate chemical ingredient of Cryptoporus vovatus . Methanol extract of Cryptoporus vovatus was analyzed by column chromatography, thin layer chromatography, HPLC, mass spectrophotometer. CVM33100Me21 was isolated by prep-HPLC from methylene chloride fraction. From the physico-chemical data, including NMR, MS, the chemical structure of the compound was determined to be an ergosterol peroxide. (Fig. 1)

본 연구에서는 확산에 의해 발생하는 시멘트 방벽의 변질 과정을 반응성용질이동 모델링을 통해 장기간 동안 예측하고자 하였다. 모델링 결과 50,000년 후 시멘트의 변질은 30cm까지 진행되었다. pH는 13.0에서 11.86까지 감소하였으며 이는 알칼리 이온의 감소, 포틀랜다이트(portlandite)와 CSH (Calcium Silicale Hydrate)광물의 용해/침전반응에 의해 결정되고 있었다. 공극률 또한 portlandite와 의 용해에 의해 가장 큰 영향을 받고 있었으며 최고 약 0.3 점도 증가하였다. 우라늄의 용해도 역시 증가하고 있었으며 이는 pe의 증가에 기인하고 있었다. 본 연구 결과는 장기간의 시멘트 변질이 pH, pe, 공극률을 변화시킴으로서 핵종의 이동을 증가시킬 수 있음을 보여주고 있다.

Background : Chrysanthemum indicum L. is used in medicine, for bee, ornamental as multivoltinism plant resources. Since ancient times, Chrysanthemum indicum L. had been widely used as drug and tea. To diversity and increase the demand of Chrysanthemum, it is breeding for medicinal herb, tea and ornamental flower. Therefore size and shape of plant, color of flower have been used for cross breeding. CI2013 lines collected from Jeju have been used for landscape and ornament. and CI2010 lines collected from Sancheong have been used for medicinal herb tea. As a result, many new lines were developed. Therefore it was carried out to use as a date for new varieties through the characterization of good lines. Methods and Results : 7 lines were investigated and these plants were forstered and cultivated from Gyeongsangnam-do Agricultural Research & Extension Services (Hamyang). Breeding Lines were CI201001, CI201002, CI201005, CI201006, CI201303, CI201307, CI201401. The shape of CI201303 and CI201307 were round, plant height is low and width is wide, it may be used for landscape and ornament. As the size of flower in CI201005 was big, it may be used for medicinal herb tea. Conclusion : CI201303 and CI201307 lines were good for landscape and ornament because of round shape. CI201005 line was good for medicinal herb tea because of big flower.

Background : Cucuma longa L., in the family Zingiberaceae, is distributed in tropical and/or sub-tropical regions mainly in India and China. This species is commonly called tumeric, powder is used as medicinal herbs and/or flavor enhancer. It has been cultivated in southern region mainly Jindo. However, it might be possible to extend cultivation region due to rise in average temperature. In order to select superior lines, agronomic characteristics is commonly used. Because this is not the ultimate solution, the DNA marker approach has benefited the modern plant breeding. Therefore an easy approach by using one kind of primer have been developed from random amplification of polymorphic DNA sequences (RAPD) to discriminate effectively between different cultivars of Cucuma species Methods and Results : DNAs were extracted from the harvested roots of Cucuma sp. using DNeasy plant Mini kit (Qiagen, Hilen, Germany). These plants cultivated from GARES (Hamyang) and used for PCR amplification. The relative concentration of the extracted DNA was estimated Nano Drop ND-1000 (NanoDrop Technologies, Wklmington, De, USA) and final DNA concentration was adjusted to 5.5 ng/㎕. In this study 9 primer pairs were tested on 8 Cucuma sp. These primers showed polymorphism in Cucuma sp. The cluster dendogram showed that the similarity coefficients ranged from 0.68 to 0.87, CUR02 turned out to be CUR11, and CUR16 is similar to CUR17. Conclusion : These finding could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Cucuma species. These data on polymorphism difference based on RAPD will be give us invaluable breeding information by selection of superior lines.

Background : The effective components of Omija(Schisandra chinensis Bailllon) are lignans (schizandrins and gomisins), and this components were contented mostly in seed part on Omija, which have various physiological functionalities such as anti-cancer, anti-inflammatory, and antioxidant activities. Methods and Results : This study was carried out to determine effective condition(CO2, CO2+ethanol) on extraction using supercritical carbon dioxide extraction (SFE) system and to find interrelation on effective components and antioxidant activity of extracts and residues obtained after extraction. Effective components were analysed lignans and phenolic compounds and antioxidant activirty was determined for DPPH radical scavenging ability on methanol extracts of SFE-extract and SFE-residue. On SFE with ethanol, SFE extract was separated two phase, upper(water phase) and lower(oil phase). SFE-extract showed the highest total lignans content(61.36 mg/g, 72.14 mg/g on lower, 50.58 mg/g on upper) and the lowest total phenolic compounds(6.52 mg/100g) and SFE-residue showed the lowest total lignans content(1.45 mg/g) and the highest total phenolic compounds(16.23 mg/100g) by extracted on CO2+ethanol treatment. SFE-residue methanol extract showed the highest DPPH radical scavenging abilities and SFE-extract upper showed the lowest. Conclusion : Thus, this results showed SFE-extract showed the highest total lignans content, but SFE-residue showed the highest DPPH radical scavenging ability although the lowest total lignans content.

This study describes results on sexual maturation and characteristics of natural spawned eggs to develop a method for the production of stable, healthy fertilized eggs from captive-reared yellowtail kingfish, Seriola lalandi. A total of 59 yellowtail kingfish were captured off the coast of Jeju Island, after which the broodstock was cultured in indoor culture tank (100 m3) until they were 6.1–14.9 kg in body weight. As part of the rearing management for induced sex maturation, the intensity of illumination was maintained at 130 lux. The photoperiod (light/dark; L/D) was set to a 12 L/12 D from October 2013 to January 2014, and 15 L/9 D from February 2014 to June 2014. Feeds comprised mainly EP (Extruded Pellets), with squid cuttlefish added for improvement of egg quality, and was given from April to June 2014. The first spawning of yellowtail kingfish occurred in May 3, 2014, at a water temperature of 17.0°C. Spawning continued until June 12, 2014, with the water temperature set at 20.5°C. Time of spawning was 26 times at this period. The total number of eggs that spawned during the spawning period was 4,449×103. The buoyant rate of spawning eggs and fertilization rate of buoyant eggs during the spawned period were 76.1% and 100%, respectively. The diameters of the egg and oil globule were 1.388 ± 0.041 mm and 0.378 ± 0.029 mm, respectively, which was higher in early eggs than in those from late during the spawned period.

This study monitored the morphological development of embryo, larvae and juvenile yellowtail kingfish, Seriola lalandi, for their aquaculture. The fertilized eggs obtained by natural spawning were spherical shape and buoyant. Fertilized eggs were transparent and had one oil globule in the yolk, with an egg diameter of 1.35 ± 0.04 mm and an oil globule diameter of 0.32 ± 0.02 mm. The fertilized eggs hatched 67–75 h after fertilization in water at 20 ± 0.5°C. The total length (TL) of the hatched larvae was 3.62 ± 0.16 mm. During hatching, the larvae, with their mouth and anus not yet opened. The yolk was completely absorbed 3 days after hatching (DAH), while the TL of post-larvae was 4.72 ± 0.07 mm. At 40 DAH, the juveniles had grown to 30.44 ± 4.07 mm in TL, body depth increased, the body color changed to a black, yellow, and light gray-blue color, and 3–4 vertical stripes appeared. At 45 DAH, the juveniles were 38.67 ± 5.65 mm in TL and 10.10 ± 0.94 mm in body depth. The fish were green with a light orange color, with 7 faint green-brown stripes on the sides of their body. At 87 DAH, the juveniles had grown to 236.11 mm in TL, 217.68 mm in fork length, and 136.5 g in weight. The fish resembled their adult form, with a light yellow-green body color, loss of the pattern on the sides of their body, and a yellow coloration at the tip of the caudal fin.

Background : Fruit picking is the most labor consuming procedure for Boxthorn (Lycium chinense Mill.) growers. Recently fruit harvesters were invented and improved in the efficiency to fulfill the expectation of farmers through field demonstration tests. Additionally, T-type hedge cultivation method harmonized well with fruit picking machine, but it also needed to be improved in its’ pruning methods for the efficiency of fruit collecting procedure. Methods and Results : In this experiment, ‘Cheong-un’ cultivar and local variety were grown in the rain shelter greenhouses for the tests. Plots were set by randomized block design. There were two types of previously developed harvesters. One vibrates it’s harvest rod right and left (A type) and the other shakes it’s rod back and forth (B type). Among two harvesters A type was superior to B type in efficiency and convenience in use and it was able to harvest eleven times more fruits than manual picking in simple comparison test. In field comparison test, A type harvester performed 4.6 to 5 times more fruits than manual fruit picking. T-type hedge cultivation method is the proper way of enhancing efficiency of harvester. In the test, wiry perpendicular branches formed by once pruning method hindered moving forward of fruit collector. However installing collecting net with velcro along the edge under the field increased the efficiency and handiness of fruit harvester and well harmonized with T-type hedge cultivation. Conclusion : From the above results, the newly developed fruit harvester could increase the annual income of Boxthorn growers by reducing fruit picking labor and time.

Background : Boxthorn has been cultivated by conventional fertilization and irrigation methods that can be characterized by applying a great deal of fertilizers and a small amount of water compared to the other crops. With increasing rain shelter greenhouses, there were significant needs for developing new fertigation and irrigation methods. In this study, several prescribed fertigation methods for balanced fertilizer application and a few irrigation start methods based on soil moisture tension has been tested to confirm the fruit quality and productivity for Boxthorn growers. Methods and Results : In the experiment, ‘Cheong-un’ cultivar was grown in the rain shelter greenhouses for the tests. Plots were set by randomized block design. According to the growth investigation on the ‘Cheong-un’ cultivar which is supplied with four different formulated fertigation, the formulation N 15 me/ℓ increased the size and weight of fruit leading to 17% increased yield than the others. Additionally, on the amount of annual supply of fertigation with the concentration of N 15 me/ℓ, the treatment applied with 10kg/10a during the cultivation period facilitated overall growth of ‘Cheong-un’ cultivar and increased the yield as well. In the irrigation experiment ‘Cheong-un’ cultivar was grown in the four differently planned treatments that start irrigation at –20, -40, -50 and -100kPa soil moisture tensions. According to the investigation, the irrigation treatment starting at -50kPa enhanced the number of fruit bearing and productivity of Boxthorn consistently during two years. Conclusion : Therefore, the newly developed fertigation and irrigation methods could enhance the annual income of Boxthorn growers by increasing growth and yield.

This study was conducted to develop a fast and efficient screening method to determine the quantity of fatty acid in peanut oil for high oleate breeding program. A total of 329 peanut samples were used in this study, 227 of which were considered in the calibration equation development and 102 were utilized for validation, using near infrared reflectance spectroscopy (NIRS). The NIRS equations for all the seven fatty acids had low standard error of calibration (SEC) values, while high R2 values of 0.983 and 0.991 were obtained for oleic and linoleic acids, respectively in the calibration equation. Furthermore, the predicted means of the two main fatty acids in the calibration equation were very similar to the means based on gas chromatography (GC) analysis, ranging from 36.7 to 77.1% for oleic acid and 7.1 to 42.7% for linoleic acid. Based on the standard error of prediction (SEP), bias values, and R 2 statistics, the NIRS fatty acid equations were accurately predicted the concentrations of oleic and linoleic acids of the validation sample set. These results suggest that NIRS equations of oleic and linoleic acid can be used as a rapid mass screening method for fatty acid content analysis in peanut breeding program.