Gentamicin is an aminoglycoside antibiotic effective against aerobic gram-negative bacteria and is also used in veterinary medicine, particularly in the swine and bovine industries. However, no gentamicin product is currently approved for treating equine diseases in Korea. The present study aims to examine the time-dependent residue of gentamicin in horses after intravenous injection (IV) via jugular vein. The test product was injected at 6.6 mg/kg BW via jugular vein in nine horses. Blood was collected from the horse's jugular vein at 15 minutes, 30 minutes, 1, 4, 8, 12, 24 and 48 hours after injection. To purify the gentamicin in serum, 100μL of 20 mM HFBA in DW, 100 μL of 30% trichloroacetic acid and 300 μL of 20 mM heptafluorobutyric acid (HFBA) in acetonitrile (ACN) were added to 500 μL of serum and supernatant was applied to LC-MS/MS after centrifugation. LC-MS/MS-8050 analyzed the level of gentamicin in serum with Electrospray ionization (ESI) and multiple reaction monitoring (MRM) positive mode. Gentamicin C1 was 478 m/z and product ions were 322, 157 m/z. Precursor ion of Gentamicin C1a was 450 m/z and product ions were 322, 160 m/z. Precursor ion of Gentamicin C2 and C2a was 464 m/z and product ions were 322, 160 m/z. The LC column was a C18 and mobile phase composed of 20 mM HFBA in 5% ACN and 20 mM HFBA in 50% ACN. The amount of gentamicin was calculated by adding four components of gentamicin (C1, C1a, C2 and C2a). The pharmacokinetic parameters of gentamicin were calculated by the WinNonlin program. The Cmax of gentamicin in horse serum was 93 ± 17 μg/kg and the Tmax was 0.25 ± 0 hours. The T1/2 was 6.41 ± 2.32 hours and the CLt was 0.05 ± 0.01L/hr/kg. The Vd was shown as 0.44 ± 0.13 L/kg and the MRT was 1.98 ± 0.55 hours. In conclusion, our data provides useful pharmacokinetic parameters for gentamicin in horses following IV injection.

국내 수박(Citrullus lanatus) 품종의 발아율에 대한 Solid Matrix Priming(SMP)의 영향을 평가하였다. Micro cel-E가 SMP 처리에 가장 이상적인 matrix로 밝혀졌으며, 종자:matix:물의 비율은 10:5:10(w/w/w)로, 25°C 에서 3일 동안 처리했을 때 유근의 돌출없이 발아속도가 촉진되었다. SMP 처리 과정 동안 초기 4시간 동안의 수분 흡수율은 급격하게 증가했으며, 이후 72시간 동안은 더 느린 속도로 흡수되었고, 처리 마지막 시간까지 수분 흡수 율은 41%이었다. 발아 온도와 상관없이, SMP 처리된 종자는 발아 시간이 단축되면서 발아율이 향상되었다. 특히, 최적 발아 온도보다 낮은 온도에서 발아율이 높았다. 품종별 차이는 있었으나, 특히 ‘해찬꿀’과 ‘리코스위트’ 품종 에서 발아율이 유의미하게 증가하였다. 또한 일부 품종에서 SMP 처리 종자의 유묘출현율과 초장, 생체중이 무처 리에 비하여 증가한 값을 보였으며, SMP 처리로 종자의 발아속도 및 유묘활력을 증진시켜 우량 묘 확보가 가능한 것으로 판단되었다.

Moringa oleifera, a versatile plant, has been traditionally used to treat various ailments and is gaining scientific attention due to its potential as a medicine. Native to the Indian subcontinent, it is widely grown in tropical and subtropical regions, thriving in Asia, Africa, and South America, especially in arid climates. This study explores the antioxidant potential of Moringa oleifera leaf extract (MOLE), employing a comprehensive screening approach with various solvents to identify the most effective extraction method. Initial experiments assessed antioxidant efficacy and yield using distilled water (D.W.), 95% ethanol, and 95% methanol. Among these, 95% ethanol extract demonstrated superior antioxidant activity, confirmed through assays such as 2,2-diphenyl-1-14 picrylhydrazyl (DPPH) radical scavenging assay, total polyphenol content analysis, and reducing power assay. In addition, with the 95% ethanol MOLE, a higher extraction efficiency was yielded compared to other solvents, making it the most effective for large-scale preparation. HPLC analysis revealed the presence of key bioactive compounds, including ellagic acid, rutin, Q-3-O, quercetin, and kaempferol. Results revealed that MOLE, prepared using 95% ethanol, exhibited remarkable antioxidant properties, attributed to its rich polyphenolic content. This research underscores the therapeutic potential of MOLE as a natural antioxidant source and highlights the importance of solvent optimization in phytochemical extractions.

Clove (Syzygium aromaticum) is a highly valued medicinal plant native to Aisa. Widely used as a spice, renowned for its medicinal properties, particularly in Ayurveda and traditional Chinese medicine. In this study, clove bud extract (CBE) was prepared at different ethanol concentrations of 50%, 80%, and 90%, respectively. The antioxidant activity of the CBE was evaluated through DPPH, polyphenol, and reducing power assays, revealing its strong antioxidant potential, with 90% ethanol being the most effective extract. HPLC analysis identified eugenol (8.7 mg/g) as the major active compound, known to possess anti-inflammatory and antioxidant properties. Given the role of oxidative stress and inflammation in atopic dermatitis (AD), the therapeutic potential of CBE was explored using a 1-chloro-2, 4-dinitrobenzene (DNCB)-induced AD mouse model. Five-week-old BALB/c mice were induced with AD by topical application of DNCB. CBE was administered topically to the affected skin (back and ear) areas for 4 weeks. The treatment of CBE significantly reduced the severity of clinical dermatitis, decreased epidermal thickness, and lowered mast cell and eosinophil infiltration in skin tissue, as observed through hematoxylin eosin staining and toluidine blue staining. The results demonstrated CBE as a promising therapeutic agent for managing AD through its regulation of skin inflammation and oxidative stress, making it a potential candidate for future treatments of inflammatory skin disorders.

Following the previous study, which investigated the pharmacological properties of the Technekitty injection (Tc-99m), the toxicity of a single intravenous administration of the Technekitty injection (Tc-99m) and the side effects that may occur at the diagnostic dose were confirmed. The Technekitty injection (Tc-99m) was administered intravenously once at a dose of 0, 0.67, 2.0, and 6.0 mCi/kg to 5 male and female rats per group. Mortality, general symptom observation, and weight measurement were performed for 2 weeks, followed by observation of autopsy findings. There were no deaths, and no statistically significant weight change was observed. No abnormal systemic signs related to the Technekitty injection (Tc-99m) were observed. These results confirmed that Technekitty injection (Tc-99m) can be safely administered intravenously at doses up to 6.0 mCi/kg. Additionally, technetium-99m at an average dose of 2 mCi (74 MBq) has been verified as a diagnostic dose without adverse effects, allowing the Technekitty injection (Tc-99m) to be used safely without side effects at this dosage. This study demonstrates that the Technekitty injection (Tc-99m) has a wide safety margin, supporting its potential for clinical application. Moreover, these findings align with the nonclinical safety standards for radiopharmaceuticals, reinforcing its utility in veterinary medicine. The Technekitty injection (Tc-99m) is expected to be applicable for clinical diagnosis as a veterinary drug in Korea.

Thyroid scanning using technetium-99m (99mTc) is the gold standard for diagnosing feline hyperthyroidism. In cats with an overactive thyroid, a thyroid scan is the most appropriate imaging technique to detect and localize any hyperfunctional adenomatous thyroid tissue. In this study, the pharmacological properties of the Technekitty injection (Tc-99m), developed as a diagnostic agent for feline hyperthyroidism using 99mTc as an active ingredient, were tested in FRTL-5 thyroid follicular cell line and ICR mice. The percentage of cell uptake of the Tc-99m in FRTL-5 thyroid cells was 0.182 ± 0.018%, which was about 6 times higher compared to Clone 9 hepatocytes. This uptake decreased by 38.2% due to competitive inhibition by iodine (sodium iodide). In tissue distribution tests by using ICR mice, the highest distribution was observed in the liver, kidneys, spleen, lungs, and femur at 0.083 hours after administration, and this distribution decreased as the compound was excreted through the kidneys, the primary excretory organ. Maximum distribution was confirmed at 1 hour in the small intestine, 6 hours in the large intestine, and 2 hours in the thyroid gland. Additionally, the total amount excreted through urine and feces over 48 hours (2 days) was 78.80% of the injected dose, with 37.70% (47.84% of the total excretion) excreted through urine and 41.10% (52.16% of the total excretion) through feces. In conclusion, the Tc-99m has the same mechanism of action, potency, absorption, distribution, metabolism, and excretion characteristics as 99mTc used for feline hyperthyroidism in the United States, Europe, and other countries, because the Technekitty injection (Tc-99m) contains 99mTc as its sole active ingredient. Based on these results, the Technekitty injection (Tc-99m) is expected to be safely used in the clinical diagnosis of feline hyperthyroidism.

Background: Hanwoo cattle farmers aim to improve calf production and reproductive efficiency. Recovery of the reproductive tract postpartum is a critical factor influencing the postpartum period and conception of breeding cows. This study aimed to precisely analyze the recovery process of the reproductive tract in primiparous Hanwoo postpartum and to establish recovery criteria. Methods: Ten primiparous Hanwoo cows were used in this study. After parturition, estrus was examined daily using visual observations and estrus detection patches. Ovarian recovery, cervical diameter, and uterine horn diameter were examined using ultrasonography four times per week. Results: The analysis revealed that the first estrus occurred at 19.1 ± 6.5 days postpartum, the first ovulation at 27.1 ± 4.5 days, and the first normal estrus cycle at 39.2 ± 6.4 days. The ovulation rate during the first estrus was 40%. A normal estrus cycle occurred in 11.1% of patients at the first ovulation. The cervix diameter recovered to 42.0 ± 3.5 mm and the uterine horn diameter to 34.4 ± 7.1 mm by 24 days postpartum, with the difference in uterine horn diameter recovering to 2.6 ± 1.2 mm by 31 days postpartum. Conclusions: This study can aid in determining the optimal breeding time for postpartum primiparous Hanwoo cow and provide foundational data for Hanwoo breeding studies.

Background: Platelet-derived growth factor receptor alpha (PDGFRα) is essential for various biological processes, including fetal Leydig cell differentiation. The PDGFRαEGFP mouse model, which expresses an eGFP fusion gene under the native Pdgfrα promoter, serves as a valuable resource for exploring PDGFRα’s expression and function in vivo. This study investigates PDGFRα expression in adult testicular cells using PDGFRαEGFP mouse model. Methods: Genotyping PCR and gel electrophoresis were used to confirm the zygosity of PDGFRαEGFP mice. Histological examination and fluorescence imaging were used to identify PDGFRα expression within testicular tissue. Immunohistochemical analysis assessed the co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 in testicular cells. Results: Genotyping confirmed the heterozygous status of the mice, which is crucial for studies due to the embryonic lethal phenotype observed in homozygotes. Histological and fluorescence imaging revealed that PDGFRα+ cells were primarily located in the interstitial spaces of the testis, specifically within Leydig cells and peritubular myoid cells (PMCs). Immunohistochemical results showed PDGFRα co-localization with c-Kit and ANO-1 in Leydig cells and a complete co-localization with TASK-1 in both Leydig cells and PMCs. Conclusions: The findings demonstrate specific expression of PDGFRα in Leydig cells and PMCs in adult testicular tissue. The co-expression of PDGFRα with c-Kit, ANO-1, and TASK-1 suggests complex regulatory mechanisms, possibly influencing testicular function and broader physiological processes.

본 연구는 소리곤충을 활용한 동물매개치료 프로그램이 노인의 우울에 어떠한 영향을 미치는지 살펴보고자 하였다. 연구대상은 I시에 위치한 I치매안심센터에서 추천을 받은 우울 척도 검사에서 5점 이상을 받은 노인 20명이며, 실험집단과 통제집단 각 10명을 선정하였다. 프로그램은 2023년 6월부터 8월까지 주 1회, 회기당 50분 씩 총 12회기를 실시하였다. 효과 검증을 위해 SPSS v.26을 활용하여 집단 간 동질성 검증(Mann-Whitney U test)을 하여 동질성이 입증이 된 후 각 집단의 사전과 사후에 노인 우울 척도 측정을 실시하고 비모수 윌콕슨 부호 순위 검정(Wilcoxon signed-rank test)으로 분석하였다. 그 결과, 실험집단의 우울 전체의 사전・사후에 유의미한 차이 가 나타났다(Z=-3.53, p＜.05). 그러나 통제집단의 우울 전체의 사전・사후에는 유의미한 차이가 나타나지 않았 다(Z=-1.06, p>.05). 따라서 본 연구는 소리곤충을 활용한 동물매개치료 프로그램이 노인의 우울에 긍정적인 영향 을 준 것으로 확인이 되었다. 이번 연구결과는 소리곤충을 활용한 정서 및 심리 치료에 대한 과학적 근거를 제시하 여 관련 분야 활성화에 기여할 수 있을 것으로 기대된다.

Background: Leydig cells, crucial for testosterone production, express ion channels like ANO1 that influence hormone secretion. This study investigates the expression and role of the Tandem of P domains in a weak inward rectifying K+ channel-related Acid-Sensitive K+-1 (TASK-1) channel in these cells, exploring its impact on testicular function and steroidogenesis. Methods: TASK-1 expression in Leydig cells was confirmed using immunostaining, while RT-PCR and Western Blot (WB) validated its expression in the TM3 Leydig cell line. The effect of a TASK-1 channel blocker on cell viability was assessed through live/dead staining and MTT assays. Additionally, the blocker’s effect on testosterone secretion was evaluated by measuring testosterone levels. Results: Immunohistochemical analysis revealed a predominant presence of TASK- 1, along with c-Kit and ANO-1, in Leydig cells adjacent to seminiferous tubules and also in Sertoli and spermatogenic cells. Expression levels of TASK-1 mRNA and protein were significantly higher in TM3 Leydig cells compared to TM4 Sertoli cells. In addition, blocking TASK-1 in TM3 cells with ML365 induced cell death but did not affect LHinduced testosterone secretion. Conclusions: These findings suggest that TASK-1 in Leydig cells is crucial for their viability and proliferation, highlighting its potential importance in testicular physiology.

본 연구는 코마개의 사용 여부에 따라 MRI 영상을 분석함으로써 숨 참기의 정확도를 향상할 도구로써 코마개가 실 효성이 있는지 검증하고자 하였다. 이를 위해 수검자에게 코마개가 없는 자연 호흡과 코마개를 사용한 구강 호흡 상태에서 호기 후 숨 참기를 지시하고 MRI 영상을 획득하였다. 영상은 2D 경사 자기장 에코 시퀀스로 획득하였고 초당 1장씩, 총 15장을 얻어 숨 참기 상태를 분석하였다. 숨 참기의 상태를 정량적으로 평가하기 위해 우측 폐 면적 을 측정하였다. 그리고 첫 번째 영상의 폐 면적을 기준으로 나머지 영상들에서 폐 면적의 절대 오차값을 구해 비교하 였다. 더불어 첫 번째 영상을 기준으로 나머지 영상들의 기하학적 유사도를 평가하기 위해 SSIM 값을 계산하였다. 실험 결과, 자연 호흡 상태에서 절대 오차는 평균 216.79 ㎟로 나타났다. 반면, 코마개를 사용한 구강 호흡의 경우 평균 55.94 ㎟로 나타나 자연 호흡과 비교하여 약 74.19% 감소하였다. SSIM 값은 자연 호흡 시 평균 0.7, 구강 호흡 시 0.76으로 나타나 기하학적 유사도가 약 6% 향상되었다. 이는 코마개 사용 시 호흡의 초기 상태를 잘 유지함 으로써 숨 참기의 정확도가 향상되어 능동적으로 호흡 상태를 조절할 수 있는 도구로써 충분한 가치가 있음을 증명한 다. 따라서 복부 MRI 검사에서 호흡에 따른 인공물을 개선하는 데 코마개가 도움을 줄 수 있으며 영상 개선 및 업무 효율을 높이는 도구로써 충분히 활용할 수 있다.

When aluminum is in an alkaline state, the aluminum oxide film surrounding aluminum is dissolved and moisture penetrates the exposed aluminum surface, causing corrosion of aluminum. At this time, hydrogen gas is generated and there is a risk of explosion due to the generated hydrogen gas. Aluminum radioactive waste is difficult to permanently dispose of because it does not meet the standards for the acquisition of low- and intermediate-level radioactive waste cave disposal facilities currently managed and operated by the Korea Nuclear Environment Corporation. However, because of this risk, it is necessary to study how to safely treat and dispose aluminum waste. In this study, overseas cases were investigated and analyzed to ensure the safety of aluminum waste disposal, and the current status of aluminum radioactive waste generated during decommissioning of the Korea Research Reactor 1&2 and a treatment plan to secure disposal suitability were presented. The process of removing a little remaining oxygen in molten steel during the reduction of iron oxide in the iron refining process is called deoxidation, and a representative material used for deoxidation is aluminum. In the case of metal melting decontamination, which is one of the decontamination processes of radioactive metal waste, a method of treating aluminum waste by using aluminum as a deoxidizer is proposed.

The radioactive waste generated within radiation-controlled areas is classified and processed according to relevant laws and regulations based on contamination levels. In cases where such radioactive waste complies with the legally defined clearance concentration or dose criteria, it is disposed of as non-radioactive waste by means of incineration, reclamation, recycling, etc. Within radiation controlled areas, various consumables are periodically replaced to ensure the proper operation of the area. It is necessary to have appropriate disposal methods for these consumables. In particular, waste items such as fire extinguishers, fluorescent lamps, batteries, and pressure vessels (hereinafter referred to as “Special Waste Type”), which may contain hazardous substances within their internal components and contents, should be considered for appropriate disposal methods that comply with nuclear safety and environmental laws. In the present case, the specified special waste type do not come into direct contact with radiation sources, and they have impermeable surfaces, which significantly reduces the risk of external contamination infiltrating the interior. However, the current method of clearance is not suitable for these items (Typically, nuclear energy-related business operators are required to classify clearance target waste based on internal and external components and demonstrate compliance with the criteria. Nevertheless, for special waste type, it is difficult to separate and measure internal and external components within the radiation-controlled area). In this case, the Clearance Procedure for special waste type applied to Korea Atomic Energy Research Institute was introduced. Additionally, we have extracted considerations for future domestic clearance of the type.

Radioactive iodine-129, a byproduct of nuclear fission in nuclear power plants, presents significant environmental and health risks due to its high solubility in water and volatility. Iodine-129, with its half-life of 1.57×1017 years, necessitates safe management and disposal. Therefore, safely capturing and managing I-129 during spent nuclear fuel reprocessing is of paramount importance. To address these challenges, various glass waste forms containing silver iodide have been developed, such as borosilicate, silver phosphate, silver vanadate, and silver tellurite glasses. These glasses effectively immobilize iodine, but the high cost of silver raises affordability concerns. This study introduces CuI·Cu2O·TeO2 glass waste forms for iodine immobilization, a novel approach. The cost-effectiveness of copper, in contrast to silver, makes it an attractive alternative. The CuI·Cu2O·TeO2 glass waste forms were synthesized with varying CuI content (x) in (1-x)(0.3Cu2O·0.7TeO2) glass matrices. Xray diffraction (XRD) confirmed amorphous structures, and X-ray fluorescence (XRF) quantified composition. X-ray photoelectron spectroscopy (XPS) and Raman spectroscopy provided insights into structural properties. Durability assessments using a 7-day product consistency test (PCT-A) and inductively coupled plasma-mass spectrometry (ICP-MS) revealed compliance with U.S. glass regulations, making CuI·Cu2O·TeO2 glasses a promising choice for iodine immobilization in radioactive waste.

본 연구는 남한 지역에서 서식하는 멸종 위기 종 2급인 왕은점표범나비의 기후 변화에 따른 서식지 변화를 분석하고자 한다. 이를 위해 단일모델의 장단점을 보완하기 위해서 생물 보전과 동물 생태학 분야에서 널리 사용 되는 앙상블 모델을 활용하여 기후변화 시나리오 자료를 이용하여 현재와 미래 기후 조건에서의 잠재적 서식지 변화를 평가하였다. 연구 결과에 따르면, 미래에는 왕은점표범나비의 서식지가 줄어들 것으로 예상되며, 이 변화 는 기온과 강수량 모두에 영향을 받을 것으로 나타났다. 특히 강수량의 계절적 변동이 가장 큰 영향을 미칠 것으로 분석되었다. 이러한 결과는 기후 변화로 인한 생물종의 서식 분포의 이해를 향상시켜 멸종 위기 종 관리와 생태계 복원과 같은 다양한 분야에서 생물다양성 증진을 위한 중요한 기초 데이터로 활용될 것으로 기대된다.