납작먹좀벌과(Platygasteridae)의 큰검정알벌속(Trissolcus)에 속하는 T. japonicus (Ashmead) (썩덩큰검정알벌, 신칭)이 과수원에서 과일 에 심한 피해를 일으키는 해충인 썩덩나무노린재의 알에 기생하는 것으로 확인되었고, 생물적 방제인자로써 가능성이 기대된다. 또한 천마큰검정 알벌(T. itoi Ryu)이 북쪽비단노린재 알을, 검은다리큰검정알벌(T. nigripedius Nakagawa)이 알락수염노린재 알을 숙주로 각각 기생하는 것이 확 인되었다. 동정을 위한 진단형질 및 영상자료와 한국에 분포하는 큰검정알벌속(Trissolcus)의 종들에 관한 숙주목록을 제공하였다.

지금까지 한국산 다듬이벌레는 3개 아목(Trogiomorpha, Troctomorpha, Psocomorpha)의 7개 과에서 14종이 알려져 있다. 채집된 재료 를 바탕으로 동정을 실시한 결과, 2개 아목에 속하는 다음과 같은 10종을 추가한다: [Troctomorpha] Amphientomidae: Ancylentomus macrourus (Li, 1997); [Psocomorpha] Amphipsocidae: Amphipsocus japonicus (Enderlein, 1906), Caeciliusidae: Valenzuela oyamai (Enderlein, 1906), Paracaecilius japonicus (Enderlein, 1906), Stenopsocidae: Cubipilis aphidiformis (Enderlein, 1906), Stenopsocus immaculatus (Stephens, 1836), Peripsocidae: Peripsocus didymus Roesler, 1939, Psocidae: Amphigerontia anchorae Li, 2002, Psococerastis tokyoensis (Enderlein, 1906), Trichadenopsocus alternatus Li, 2002. 이 미기록종들에 대한 성충 및 진단형질의 사진을 제공하였다.

Coccoidea are some of the most potentially detrimental pests for agriculture and ornamental plant industries worldwide, including in Laos. Between January 2015 and August 2016, scale insects were collected on variety of hosts. Previously, the known Coccoidea fauna of Laos comprised 35 species. These most recent collections boost the tally to 49 species belonging to 5 families of Coccoidea. Among these, 19 species are here recorded from Laos for the first time: [Coccidae] Ceroplastes floridensis,Ceroplastes rubens, Coccus capparidis, Drepanococcus chiton, Megapulvinaria maxima, Pulvinaria psidii, Saissetia coffeae; [Diaspididae] Aonidiella citrina, Aonidiella inornata, Fiorinia coronate, Lepidosaphes tokionis, Parlatoria proteus; [Lecanodiaspididae] Psoraleococcus multipori; [Monophlebidae], Icerya aegyptiaca; [Pseudococcidae], Phenacoccus madeirensis, Planococcus ficus, Pseudococcus aurantiacus, Pseudococcus baliteus and Rastrococcus jabadiu. All of these newly recorded species occur elsewhere in south-east or east Asia, including countries contiguous with Laos.

Suborder Psocomorpha belongs to Psocoptera is one of minor order. Psocids occupy a wide range of habitats, from living as indoor, if outdoor most are presented on bark or foliage of dead or leaving tree and shrubs. All specimens of Psocomorpha that found in the field were putted into 75% of alcohol. Preparation and dissection were done before identification. Previously the list of Psocomorpha comprised 14 species. This most recent collection found nine species. Among of them, two species belong to two families; [Amphipsocidae] Amphipsocus japonicas (Enderlein, 1906) and [Psocidae] Psococerastis tokyoensis (Enderlein, 1906) are report in Korea in the first time.

Since first reported in 1988 the Pine Wilt Disease has been established and spreaded nearly nationwide in South Korea, causing tremendous economic damage in pine forests. The pine wilt nematode (Bursaphelenchus xylophilus), causing the Pine Wilt Disease, in Korea is known to be transported by two insect vectors that are Monochamus alternatus Hope and Monochamus saltuarius Gebler. Currently the pest management strategy largely relies on pesticide application and infected tree clearing. The aim of the present study was to search for early larval parasitoids of M. alternatus using the sentinel log infested with the cerambycid eggs. Only one braconid species was collected and identified as Spathius verustus Chao. Two to ten wasp larvae attached and fed on one host larva. Parasitism rate was 55.5 to 59 % in Jinju site, while 1 to 18.1 % the other sites. Sex ratio was female-biased (female : male = 18.9 : 1). The S. verstus has potential to be use as parasitic natural enemy of M. alternatus.

Spathius exarator의 종 그룹에 속하는 S. ibarakius Belokobylskij et Maeto가 한반도에서 알락하늘소(Anoplophora chinensis)의 1령과 2령 유충에 외부기생하는 것으로 확인되었다. 산란유인목을 이용하여 유리알락하늘소와 알락하늘소의 기생봉을 탐색하는 과정에서 이 고치벌이 발견 되었다. 이 고치벌의 진단형질과 수컷의 특징, 삽화 및 한반도에 분포하는 종들의 기주목록을 함께 제공하였다.

캄보디아와 라오스에는 지금까지 가루깍지벌레과 25종 및 18종이 각각 보고되어 있다. 2015년 이들 지역에서 가루깍지벌레과14종이 채집 되었으며, 그 중 캄보디아에서 2종[Antonina graminis (Maskell, 1897), Coccidohystrix insolita (Green, 1908)], 라오스에서 5종[Nipaecoccus viridis (Newstead, 1894), Paracoccus marginatus Williams & Granara de Willink, 1992, Phenacoccus solani (Ferris, 1918), Rastrococcus iceryoides (Green, 1908)]을 처음으로 보고한다.

Coccoidea (scale insects and mealybugs) are some of the most potentially detrimental pests for agriculture and ornamental plant industries in worldwide and the same in Cambodia and Laos. Scale insects were collected on branches, leaves, peduncles, petioles and fruits of hosts in January to July, 2015 and February, 2016. Previously, Coccoidea fauna of Cambodia and Laos comprise 41 and 35 species respectively. Recently we collected 25 species belong to 3 families of Coccoidea. Among these, nine species are first found in these countries; [Coccidae] Ceroplastes ceriferus (Fabricius, 1798) in Laos, Ceroplastes stellifer (Westwood 1871) in Cambodia, Milviscutulus mangiferae (Green, 1904) in Laos and Cambodia, [Pseudococcidae] Kiritshenkella sacchari (Green, 1900), Phenacoccus parvus (Morrison, 1924 ) in Laos, Palmicultor lumpurensis (Takahashi, 1951) in Cambodia, Rastrococcus invadens (Williams,1986) in Cambodia and Laos, [Diaspididae] Aulacaspis tubercularis (Newstead, 1906) in Cambodia, Aonidiella aurantii (Maskell, 1879) in Laos and Cambodia.

국내 미기록종 Pyemotes moseri Yu et Liang (Acarina: Pyemotidae)는 곤충기생성 응애로 매실 씨앗 속 유충에서 처음 발견되었다. 발견 당시 응애가 기생하고 있었던 기주의 미토콘드리아 유전자를 증폭하여 염기서열을 분석한 결과 복숭아씨살이좀벌의 유충으로 밝혀졌다. 본 연구 는 실험실 조건하에서 P. moseri의 번식과 기생 능력을 조사하였다. 복숭아씨살이좀벌의 유충을 기주로 이용하여 P. moseri의 교미한 암컷 성충을 사육하면서 주기적으로 관찰하여 새로 태어나는 자손의 수와 성별을 조사한 후 제거하였다. 기생 능력 조사는 원예용 상토가 깔린 스테인레스 바 트에 대량 사육한 응애가 들어 있는 튜브와 복숭아씨살이좀벌 유충이 가해한 매실 씨앗을 함께 담은 후 지퍼백에 넣어 인큐베이터에 보관하였다. 복숭아씨살이좀벌 유충 또는 번데기에 응애의 기생 여부를 확인하고자 한달 후 매실 씨앗을 조사하였으며 본 실험은 5반복씩 3회 실시하였다. 교 미한 암컷이 기생하기 시작한 이후부터 생식이 끝날 때까지의 기간은 평균 24.4일(n=8)이었으며 교미한 암컷 한 마리당 평균 104.0마리(n=8)의 암컷을 낳았디. 복숭아씨살이좀벌의 유충 또는 번데기가 들어 있는 매실 씨앗은 바트당 평균 7개 이상이었고 이중 단 2개의 씨앗에서만 기생에 성 공한 응애를 관찰하였다.

한반도 하늘소과의 미기록 Parechthistatus속을 처음 확인하고, Parechthistatus gibber (Bates)의 한반도 분포를 보고한다. 성충 및 유충의 사진 및 서식처 등 형태학적 정보와 생태학적 정보를 함께 제공한다.

Previously study of mealybug faunas of Cambodia and Laos were known to comprise 25 species and 18 species, respectively. Recently, we have collected fourteen mealybug species in Cambodia and Laos from June to July, 2015. Among them, six species are first found in these countries: Coccidohystrix insolita (Green) in Cambodia; and Antonina graminis (Maskell) in both countries; Nipaecoccus viridis (Newstead), Paracoccus marginatus (Williams & Granara de Willink) Phenacoccus solani (Firris) and Rastrococcus iceryoides (Green) in Laos.

한국산 거저리과의 미기록인 Palorus Mulsant에 속하는 2종인 넓은이마쌀도둑거저리(신청)(Palorus ratzeburgii (Wissman, 1848))와 좁은이마쌀도둑거저리(신칭)(P. subdepressus (Wollaston, 1864))를 보고한다. 이들 2종은 열대 및 아열대 지역에서 저장곡물해충으로 중요하지만, 국내 정미소에서는 아주 낮은 밀도로 발생하고 있다. 이들 미기록종에 대한 성충 사진, 진단형질, 저장곡물의 검역해충인 본 속의 6종에 대한 검색표를 제공하였다.

가루깍지벌레는 열대지역에서 일반적인 해충으로 관상식물과 과수류의 큰 문제를 일으키는 해충이다. 캄보디아와 라오스에는 현재 가루깍지벌레과 22종 및 12종이 각각 보고되어 있다. 최근 이들 지역에서 가루깍지벌레과 연구를 수행하여 13종이 채집되었으며, 그 중 캄보디아에서 3종인 Dysmicoccus neobrevipes (Beardsley, 1959), Phenacoccus solani (Ferris, 1918), Rastrococcus iceryoides (Green, 1908) 와 라오스에서 6종인 Dysmicoccus neobrevipes (Beardsley, 1959), Paracoccus invectus (Williams, 2004), Phenacoccus solenopsis (Tinsley, 1898), Planococcus litchi (Cox, 1989), Planococcus minor (Maskell, 1897), Pseudococcus jackbeardsleyi (Gimpel & Miller, 1996)가 이들 국가로부터 처음 보고된다.

The genus Sphathius is belong to the subfamily Doryctinae in the family Braconidae and are described with >300 species in the world (Shenefelt and Marsh, 1976). This genus is distinguished from other winged Doryctinae by 1) forewings with three submarginal cells; 2) first subdiscal cell of forewing closed; and 3) first metasomal segment petiolate, widening apically, lacking basal wing-like projections, and inserted on propodeum near coxal bases (Marsh, 1997). For the purpose of finding the biological agent for Asian longhorned beetle (ALB; Anoplophora glabripennis (Motschulsky)) and Citrus longhorned beetle (CLB; A. chinensis (Förster)), we had installed the ALB- and CLB-infested sentinel logs to attract parasitoids at experimental field of the Korea National Arboretum during summer season in 2014. Several L1 and L2 CLB larvae were found attacked by braconid larvae that were reared to adult stage. Their larvae are identified to Spathius gwangneungensis sp. nov. belong to the Spathius exarator species group. This new species has special characteristics as following; Body reddish brown or dark reddish brown, body length 2.5~3.7mm and forewing length 2.2~3.0mm in female and body length 2.1~2.6mm and forewing length 1.5~2.0mm in male, hind femur distinctly transversely aciculate dorsally, forewing more or less infuscate with a longitudinal hyaline band medially, vein m-cu of hind wing interstitial, second tergite entirely sculptured without striation, and third to fifth tergites basally sculptured. Ovipositor sheath 0.6 times as long as metasoma.

The family Pseudococcidae or mealybugs (Hemiptera) are common pests in tropical region of the world and also considered as important pests on ornamental plants and fruit trees. The mealybug faunas in Cambodia and Laos were known to nineteen species and eight species, respectively (ScaleNet, 2014; CABI, 2015; FAO-IPM, 2014). Recently, we have collected thirteen mealybug species in Cambodia and Laos. Among them, seven species of mealybug are found for the first time: Phenacoccus solani (Ferris, 1918) and Rastrococcus iceryoides (Green, 1908) in Cambodia; Dysmicoccus neobrevipes (Beardsley, 1959) and Phenacoccus solenopsis (Tinsley, 1898) in both counties; Paracoccus invectus (Williams, 2004), Planococcus minor (Maskell, 1897) and Pseudococcus jackbeardsleyi (Gimpel & Miller, 1996) in Laos.

매실의 낙과 피해를 일으키는 복숭아씨살이좀벌은 경기도, 충청남북도, 전라남북도 지역의 매실과 살구, 그리고 중국산 복숭아 종자에서 확인되었다. 복숭아씨살이좀벌은 땅에 떨어진 핵과류의 핵 속에서 유충 상태로 월동하고, 노숙유충은 4월 중순까지, 번데기는 3월 하순에서 4월 하순까지 관찰되었으며, 성충은 4월 하순~5월 상순 사이에 90% 이상이 우화하는 것으로 조사되었다. 암컷 성충은 핵이 경화되기 이전 씨앗이 액 상일 때 과실 씨앗의 외피 바로 밑에 산란하였으며, 산란관의 길이가 5 mm를 넘지 않아 산란이 가능한 매실의 크기는 직경이 2 cm를 넘지 않았 다. 하나의 과실에 최대 5개의 알이 관찰되었으며, 알 기간은 2일을 넘지 않았다. 부화한 유충은 먼저 고형화되는 배로 이동하여 이를 섭식하면서 성장하는데 이 과정에서 유충들 사이의 경쟁으로 인해 한 마리의 유충만 생존하였다. 6월 상순경 피해를 받은 과실의 대부분이 부패증상을 보이 며 낙과하였다. 땅에 떨어진 과실의 핵 속에서 이듬해 봄까지 노숙유충으로 월동하였다.

Members of the order Psocoptera are known as psocids (commonly called booklice or bark lice). There are more than 5,500 species in 41 familes in three suborders. The suborder Psocomorpha is the largest suborder in Psocoptera, containing 25 of the 41 psocopteran families. Psocomorphan familes are classified into four groups: Epipsocetae, Caeciliusetae, Homilopsocidea and Psocetae. Families Caeciliusidae and Stenopsocidae belong to the group Caeciliusetae. The family Caeciliusidae is the largest family of Psocomorpha, and is widely distributed in all zoogeographical regions. Monophyly was not supported by any autapomorphy (Yoshizawa, 2002). The Stenopsocidae is distributed in the Afrotropical, Palearctic, Oriental, and Australian regions. Monophyly is well supported by the presence of the R1-R2+3 crossvein, M-CuA1 crossvein, and lateral pouch of the spermathecal sac (Yoshizawa, 2002). These families, Caeciliusidae and Stenopsocidae consist of about 676 described species in 32 genera and about 188 described species in 4 genera, respectively. In Asia, there were reported 337 species belonging to 11 genera and 154 species belonging to 4 genera from China (Li, 2000) and 22 species belonging to 6 genera and 7 species belonging to 2 genera from Japan (Yoshizawa, 2004), respectively, but there have not been known from Korea until now. This study is the first work to make the checklist of the Korean Psocoptera. Through this study, I have reported 2 species of families Caeciliusidae and Stenopsocidae new to Korea, respectively. Family Caeciliusidae Valenzuela oyamai (Enderlein, 1906) Paracaecilius japonicus (Enderlein, 1906) Family Stenopsocidae Cubipilis aphidiformis (Enderlein, 1906) Stenopsocus immaculatus (Stephens, 1836)

Surveillance for subtropical moths through the inanimate pathway in the Korean Peninsula was investigated during the past seven years using the light trap in Baengnyeong and Daecheong islands, located near the Northern Limit Line (NLL) in the West Sea. Each record typically includes the taxonomic identify, its country of origin, and information related to inanimate pathway. A total of 2,201 individuals were detected, comprising 25 families, 441 species of Lepidoptera. The most dominant species was Chionarctia nivea (Noctuidae) with 44 individuals, followed by a crambid moth, Ostrinia furnacalis with 37 individuals. Among them, two species of subtropical moths, including Ischyja manlia Cramer and Spirama sp. belonging to the family Noctuidae, and they are mostly distributed in the subtropical area from the southern part of China to Borneo or Java. Interestingly, the other three species, Acronicta pruinosa (Guenée), Marumba spectabilis (Butler), and Spodoptera pecten Guenée were distributed in the southern regions, however, due to the effects of global warming, biotic province is now considered that may be certified moving northwards. Thus, it is very important to our weather conditions and further study on the surveillance of subtropical or/and southern species is needed, considering its possible status as a pest insect.

Metcalfa pruinosa has been spreading in Korea since 2005, which its first report at Gimhae in Kyeongnam province. It has been harmful to grape and major forest crops by direct sucking and indirect transmitting sooty mold disease causing economical loss. It is necessary to study its tracing route and movement conditions for the further efficient population management and prevention of its re-invasion. A total of 23 haplotype were observed in the analysis of nucleotide polymorphisms on mitochondrial cytochrome c oxidase I from total 124 voucher specimens among five countries. Only two haplotypes were exist in Korea and HAP1 was accorded with its of some European individuals. Moreover, the analysis of FST and AMOVA, the Korean population was relatively nearer with Spanish and Italian population than American populations, suggesting the Korean population might be originated from some European countries. Eight microsatellite loci were developed and characterized to facilitate more delegate population genetic analysis from 468 individual in five countries. The average character of each or overall population was revealed 18 average individual number, six alleles and 0.676 heterozygosity. The genetic distance (FST) was a little bit high among each populations ranged as – 0.010~0.245. In the tracing route analysis, the originating country of Korean population was also shown as migrated from some European countries. The population genetic analysis using genetic markers will be useful to trace the origin of pest and prevent from re-invasion fro efficient pest management.

Lepidopteran hitchhikers were investigated in vessel at five major ports of Korea in 2013. These moths were collected by International Plant-Quarantine Accreditation Board (IPAB) during inspection on vessels for Asian Gypsy Moth (AGM). The results typically include the taxonomic identification, their origin localities, and information related to commodities and ship’s route. A total of 1,026 individuals were detected in vessels and identified with 236 species belonging to 21 families. Although most of the species are already known to the Korean fauna, the following three species are unknown: Lymantria xylina Swinhoe (Lymantriidae), Glyphodes actorionalis Walker (Crambidae), and Histia flabellicornis nigrinus Jordan (Zygaenidae). Interestingly, H. flabellicornis nigrinus Jordan was detected for two consecutive years. Although this species is not an important pest, it will be need to necessarily continual monitoring. L. xylina Swinhoe which is a great potential forest pest, was detected four times (16 specimens) at Busan port of entry. Therefore, it is necessary to provide comprehensive border surveillance monitoring and a valuable historical record of the array of lepidopteran hitchhikers transported to the Korea through inanimate pathway, in order to counter further potential importance of hitchhikers on international vessels at Korea.