연구에서는 '누리금잠'이라 명명된 새로운 누에 신품종을 개발하였으며, 이는 황색 고치와 세리신 고치를 생산 하는 두 개의 기존 계통, 잠311과 D751의 교배를 통해 육성되었습니다. 이 신품종은 첫 교배 실험을 거친 2019년 봄부터 2021년 봄까지 총 4차례의 생산력 평가를 진행했으며, 이어진 2022년 봄부터 2023년 가을까지 4차례에 걸친 지역 적응성 평가를 통해 2023년 가을에 새로운 품종으로 공식 인정받았습니다. '누리금잠'은 봄과 가을 시즌에 각각 평균 부화율 86.9%, 89.6%를 기록하였고, 유충의 평균 성장 기간은 봄에는 21일과 12시간, 가을에는 19일과 22시간으로 나타났습니다. 세리신 고치의 평균 생산성은 봄에 79.17%, 가을에 74.9%였으며, 수확된 세리 신의 평균 중량은 누에고치 250개를 기준으로 봄에 6g, 가을에는 7.7g으로 측정되었습니다. 이와 같은 결과는 '누리금잠'이 높은 부화율과 우수한 세리신 생산성을 갖추고 있음을 시사합니다. 이는 세리 신 기반 제품의 생산 효율성을 증가시킬 뿐만 아니라, 양잠산업의 경제적 가치를 상승시키는 데 기여할 수 있을 것으로 생각됩니다.

The demand for transportation is increasing due to the continuous generation of radioactive wastes. Especially, considering the geographical characteristics of Korea and the location characteristics of nuclear facilities, the demand for maritime transportation is expected to increase. If a sinking accident happens during maritime transportation, radioactive materials can be released into the ocean from radioactive waste transportation containers. Radioactive materials can spread through the ocean currents and have radiological effects on humans. The effect on humans is proportional to the concentration of radioactive materials in the ocean compartment. In order to calculate the concentration of radioactive materials that constantly flow along the ocean current, it is necessary to divide the wide ocean into appropriate compartments and express the transfer processes of radioactive materials between the compartments. Accordingly, this study analyzed various ocean transfer evaluation methodologies of overseas maritime transportation risk codes. MARINRAD, POSEIDON, and LAMER codes were selected to analyze the maritime transfer evaluation methodology. MARINRAD divided the ocean into two types of compartments that water and sediment compartments. And it was assumed that radionuclides are transfered from water to water or from water to sediment. Advection, diffusion, and sedimentation were established as transfer process for radionuclides between compartments. MARINRAD use transfer parameters to evaluate transer processes by advection, diffusion, and sedimentation. Transfer parameters were affected by flow rate, sedimentation rate, sediment porosity, and etc. POSEIDON also divided the ocean into two types that water and sediment compartment, each compartments was detaily divided into three vertical sub-compartment. Advection, diffusion, resuspension, sedimentation, and bioturbation were established as transport processes for radionuclides between compartments. POSEIDON also used transfer parameters for evaluating advection, diffusion, resuspension, sedimentation, and bioturbation. Transfer parameters were affected by suspended sediment rates, sedimentation rates, vertical diffusion coefficients, bioturbation factors, porosity, and etc. LAMER only considered the water compartment. It divided the water compartment into vertical detailed compartments. Diffusion, advection and sedimentation were established as the nuclide transfer processes between the compartments. To evaluated the transfer processes of nuclides for diffusion and advection, LAMER calculated the probability with generating random position vectors for radionuclides’ locations rather than deterministic methods such as MARINRAD’s transfer parameters or POSEIDON’s transfer rates to evaluate transfer processes. The results of this study can be used as a basis for developing radioactive materials’ ocean transfer evaluation model.

In general, after the decommissioning of nuclear facilities, buildings on the site can be demolished or reused. The NSSC (Nuclear Safety and Security Commission) Notice No. 2021-11 suggests that when reusing the building on the decommissioning site, a safety assessment should be performed to confirm the effect of residual radioactivity. However, in Korea, there are currently no decommissioning experiences of nuclear power plants, and the experiences of building reuse safety assessment are also insufficient. Therefore, in this study, we analyzed the foreign cases of building reuse safety assessment after decommissioning of nuclear facilities. In this study, we investigated the Yankee Rowe nuclear power plant, Rancho Seco nuclear power plant, and Hematite fuel cycle facility. For each case, the source term, exposure scenario, exposure pathway, input parameter, and building DCGLs were analyzed. In the case of source term, each facility selected 9~26 radionuclides according to the characteristics of facilities. In the case of exposure scenario, building occupancy scenario which individuals occupy in reusing buildings was selected for all cases. Additionally, Rancho Seco also selected building renovation scenario for maintenance of building. All facilities selected 5 exposure pathways, 1) external exposure directly from a source, 2) external exposure by air submersion, 3) external exposure by deposited on the floor and wall, 4) internal exposure by inhalation, and 5) internal exposure by inadvertent ingestion. For the assessment, we used RESRAD-BUILD code for deriving building DCGLs. Input parameters are classified into building parameter, receptor parameter, and source parameter. Building parameter includes compartment height and area, receptor parameter includes indoor occupancy fraction, ingestion rate, and inhalation rate, and source parameter includes source thickness and density. The input parameters were differently selected according to the characteristics of each nuclear facility. Finally, they derived building DCGLs based on the selected source term, exposure scenario, exposure pathway, and input parameters. As a result, it was found that the maximum DCGL was 1.40×108 dpm/100 cm2, 1.30×107 dpm/100 cm2, and 1.41×109 dpm/100 cm2 for Yankee Rowe nuclear power plant, Rancho Seco nuclear power plant, and Hematite fuel cycle facility, respectively. In this study, we investigated the case of building reuse safety assessment after decommissioning of the Yankee Rowe nuclear power Plant, Rancho Seco nuclear power plant, and Hematite fuel cycle facility. Source terms, exposure scenarios, exposure pathways, input parameters, and building DCGLs were analyzed, and they were found to be different depending on the characteristics of the building. This study is expected to be used in the future building reuse safety assessment after decommissioning of domestic nuclear power plants. This work was

Deep geological disposal (DGD) of spent nuclear fuels (SNF) at 500 m–1 km depth has been the mainly researched as SNF disposal method, but with the recent drilling technology development, interest in deep borehole disposal (DBD) at 5 km depth is increasing. In DBD, up to 40SNF canisters are disposed of in a borehole with a diameter of about 50 cm, and SNF is disposed of at a depth of 2–5 km underground. DBD has the advantage of minimizing the disposal area and safely isolating highlevel waste from the ecosystem. Recently, due to an increasing necessity of developing an efficient alternative disposal system compared to DGD domestically, technological development for DBD has begun. In this paper, the research status of canister operation technology and plans for DBD demonstration tests, which subjects are being studied in the project of developing a safety-enhancing high-efficiency disposal system, are introduced. The canister operation technology for DBD can be divided into connection device development and operation technology. The developing connection device, emplacing and retrieving canisters in borehole, adopted the concept of a wedge thus making replacement equipment at the surface unnecessary. The new connection device has the advantage of being well applied with emplacement facilities only by simple mechanical operation. The technology of operating a connection device in DBD can be divided into drill pipe, coiled tubing, free-drop, and wireline. The drill pipe is a proven method in the oil industry, but requiring huge surface equipment. The coiled tubing method uses a flexible tube and shares disadvantages as the drill pipe. The free-drop is a convenient method of dropping canister into a borehole, but has a weakness in irretrievability in an accident. Finally, the wireline method can be operational on a small scale using hydraulic cranes, but the number of operated canisters at once is limited. The test facility through which the connection device is to be tested consists of dummy canister, borehole, lifting part, monitoring part, and connecting device. The canister weight is determined according to the emplacement operation unit. The lifting part will be composed following wireline consisting of a crane, a wire and a winding system. The monitoring part will consist of an external monitoring system for hoists and trolleys, and an internal monitoring system for the connection device’s location, pressure, and speed. In this project, a demonstration test will be conducted in a borehole with 1km depth, 10 cm diameter provided by KAERI to verify operation in the actual drilling environment after design improvement of the connecting device. If a problem is found through the demonstration test, the problem will be improved, and an improved connection device will be tested to an extended borehole with a 2 km depth, 40 cm diameter.

Pine wilt disease (PWD) is one of the most important forest tree diseases, especially in the East Asian countries of Japan, China, and Korea. The Japanese pine sawyer, Monochamus alternatus Hope, is the PWN vector for Japanese red pine and Japanese black pine while Monochamus saltuarius Gebler is the vector for Korean white pine. Various control methods, such as aerial pesticide applications, biological control using parasitoids and fumigant such as methyl bromide are used. But the PWD still has spread. Therefore, we were selected effective aerial insecticides. Susceptibility of M. saltuarius and M. alternatus adults were investigated using 9 insecticides which are available in the market in Korea. And then, we tested them to the Apis mellifera to vertify the environmental impact.

Monochamus saltuarius is one of the insect vector of pine wilt disease with Monochamus alternatus. The present study aimed to investigate the dose to induce sterile and the effect of sterility according to the gender of M. saltuarius. As the electron beam irradiation dose increased, the hatchability, adult eclosion rate and longevity of the former mid and late maturation feeding stage of adults was slightly reduced. However, there was no difference between these two adults. In addition, female adults were more sensitive to the electron beam than the male adults. Hatchability of the F1 generation of ♂T × ♀N o r♀T × ♂N (T: 200 Gy electron beam irradiation, N: 0 Gy) was completely suppressed. As electron beam irradiation dose increased, the fecundity of female adults were slightly decreased. Results by comet assay showed that electron beam irradiation induced as dose increased DNA damage in M. saltuarius adults. These results suggest that SIT using electron beam may be useful for control of M. saltuarius.

Monochamus alternatus is one of the insect vector of pine wilt disease with Monochamus saltuarius. The present study aimed to investigate the dose to induce sterile and the effect of sterility according to the gender of M. alternatus. As the electron beam irradiation dose increased, the longevity of the mid and late maturation feeding stage of adults was slightly reduced. However, there was no difference between these two adults. In addition, female adults were more sensitive to the electron beam than the male adults. Hatchability of the F1 generation of ♂T×♀N or ♀T×♂N (T: 200 Gy electron beam irradiation, N: 0 Gy) was completely suppressed. As electron beam irradiation dose increased, the fecundity of female adults were slightly decreased. Results by comet assay showed that electron beam irradiation induced as dose increased DNA damage in M. alternatus adults. These results suggest that SIT using electron beam may be useful for control of M. alternatus.

Attacin is a well-studied glycine-rich antibacterial protein in insect immune response, which has limitary antibacterial effect to some Gram-negative bacteria. A cDNA encoding the attacin gene was screened and isolated from the immunized larvae of the swallowtail butterfly, Papilio xuthus. The complete P. xuthus attacin cDNA is 949 nucleotides encoding a 250 amino acid precursor that contains a putative 18-residue signal peptide, a common 42-residue propeptide sequence and a presumed 190-residue mature protein with a theoretical mass of 19904.01 and a pI of 9.13. The putative mature protein of P. xuthus attacin showed 48%~52% and 24%~30% identity in amino acid sequences with that of lepidopteran and dipteran insects, respectively. The attacin transcript was induced at significant level after injection with bacterial lipopolysaccharide (LPS). Recombinant attacin was highly expressed in E. coli BL21 (DE3) cells by fusing with an N-terminal S-tag/thrombin cleavage site configuration protein to avoid the cell death during induction. The expressed fusion protein was purified by Ni-NTA immobilized metal affinity chromatography (IMAC). After desalting and cleavage with thrombin, the recombinant attacin was released and showed considerably antibacterial activity against Gram-negative bacteria, E. coli ML 35. Our results proved that this protein family with a potent antibacterial activity may play a role in the immune response of butterflies.

Carotenoids are major secondary compounds in Citrus determining the color of fruit and nutritional values. Carotenoids are isoprenoic compounds, and function as color pigments in the flower and fruit to attract pollinators and seed-dispersing animal and chromophore for light harvest and photoprotectant during photosynthesis. In the aim of developing new cultivars with high value using molecular breeding technology, we had performed screening of flesh and peel specific genes by differentially expressed gene screening in Citrus unshiu fruits. From the screening, carotenoid isomerase (CRTISO)1, which converts pro-lycopene to all-trans-lycopene, was identified as peel-specifically expressed gene. In this study, the gene encoding the CRTISO1 was cloned, sequenced, and compared to the CRTISOs in other plant species. Comparison of the cds sequence to other plant species revealed 75% and 78% identity with CRTISO1 of Zea maize and CRTISO2 of Arabidopsis thaliana respectively. We also cloned CRTISO2 from C. unshiu which declines the expression while maturation (Kato et al., 2004), and the gene structure was analyzed. This is the first work reporting the full sequence and gene structure of CRTISOs in C. unshiu, and would give important information in understanding the carotenoid synthesis in the Citrus fruit.

A new six-rowed naked waxy barley variety, ‘Saehanchal’, was developed by the barley breeding team of the National Institute of Crop Science (NICS), R.D.A. This variety was derived from a cross between ‘SB7803G-BC6-B-B-47-2’ and ‘Suwon262’ in 1989. The fi