Under natural conditions, black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), colonizes in warm temperateregions. Information on black soldier fly rearing, which is affected by seasonal factors in Korea, is limited. Ovipositionby black soldier flies is dependent on light intensity and wavelength. Therefore, continuous mass rearing of this fly requiresdetermination of optimal artificial conditions of illumination. In this study, we compared the number of eggs laid underan artificial light source (750 watt HPL lamp) versus nature sunlight. Our results showed that compared to ovipositionunder natural sunlight, the use of one or two lamps for 7 hours, resulted in only 43 and 76%, of the total number ofeggs laid under natural sunlight, respectively. The hatching rate under the former was much significantly lower than underthe latter.

A Duplex polymerase chain reaction (PCR) was developed for the simultaneous detection and differentiation among Nosema spp. and Vairimorpha spp. from Lepidoptera insects. Two sets of primers were selected from different genomic sequences to specifically amplify an 831 bp amplicon within the SSU rRNA gene, specific for both Nosema spp. and Vairimorpha spp. (MSSR primer); a 542 bp amplicon within the SSU rRNA gene, specific for Vairimorpha spp. (VSSU primer). Using the primers in conjunction (duplex PCR) it was possible to detect Nosema spp. and Vairimorpha spp. to differentiate between them. The sensitivity of this PCR assay was approximately 10 spores per milliliter. It is proposed that the duplex PCR is a sensitive, specific and rapid tool that can serve as a useful differential diagnostic tool for detecting Nosema spp. and Vairimorpha spp. in Lepidoptera insect.

The black soldier fly, Hermetia illucens (BSF), has a worldwide distribution in the tropics and warm temperate regions and is active in the Korea from May through October. This species colonize a wide variety of decomposing vegetable and animal matter and oviposit in a variety of decomposing materials. In this study, how the BSF molting, adult emergence and mating rate changed by seasonal condition at the artificial rearing system was investigated. The black soldier fly larvae and pupae were reared under laboratory condition (27℃, 60% R.H.). Under the laboratory condition, molting and adult emergence were not influenced by seasonal factors such as climate, radiation intensity. But it is known that the sunlight is the most important factor of the mating. In the previous study the time of day, temperature, and humidity is significantly correlated with oviposition and mating. The rearing of BSF throughout the year is restricted by sunlight. In this study, the data shows definitely different mating numbers throughout whole year. The time of the day and sunlight density are changed with season and it influence on artificial rearing. To culture the black soldier fly throughout the year in Korea needs a more deep study under the artificial rearing system.

Protease from various sources have been studied biotecnologically. For biotechnological applications, one highly preferred enzyme is protease. There have been no reports of cloned genes encoding digestive proteases in the Laccotrephes japonenis, Ranatra unicolor, Muljarus japonicus. These insects are considered to be a predator of aquatic insects. RT-PCR was used to amplify cDNA fragments for digestive proteases from total RNA the hole body of the insects. The flanking sequences of the 5'- and 3'- end of the these genes were characterized by RACE-PCR. Sequence analysis showed that these genes contained complete ORF. The deduced amino acid sequences of these protease showed 62% identity to the serine protease of Creontiades dilutus, 58% to Lygus loneolaris trypsin-like serine proteinase, 54% to Triatonatoma infestans salivary trypsin. To generate Laccotrephes japonensis serine protease, the DNA fragment coding for serine protease is cloning into suttle vector pBACⅠ, named pBAC1-JG and infected to Spodoptera frugiperda (sf9) insect cell. The cDNA encoding JG was expressed as a 32-kDa polypeptide in baculovirus infected insect cells and the recombinant protein showed activity in the protease enzyme assay using gelatin as a substrate.

The morecular cloning, gene structure, expression and enzyme activity of a serine-like proteas frome Laccotrephes Japonensis were examined. In this study, RT-PCR was used to amplify cDNA fragments for serine-like proteases from total RNA the hole body of Laccotrephes japonensis. The flanking sequences of the 5'- and 3'- end of the this gene were characterized by RACE-PCR. Sequence analysis showed that this gene contained an 963bp ORF encoding 321 amino acids. The deduced amino acid sequence of this protease showed 62% identity to the serine protease of Creontiades dilutus, 58% to Lygus loneolaris trypsin precuror LlsgP4, 54% to Triatonatoma infestans salivary trypsin. To generate Laccotrephes japonensis serine-like protease, the DNA fragment coding for serine protease is cloning into suttle vector pBACⅠ, named pBAC1-JG and infected to Spodoptera frugiperda (sf9) insect cell. The cDNA encoding JG was expressed as a 32-kDa polypeptide in baculovirus infected insect cells and the recombinant JG showed activity in the protease enzyme assay using gelatin as a substrate.

Protease from various sources have been studied biotecnologically. For biotechnological applications, one highly preferred enzyme is protease. There have been no reports of cloned genes encoding digestive proteases in the Laccotrephes japonenis, Ranatra unicolor, Muljarus japonicus. These insects are considered to be a predator of aquatic insects. RT-PCR was used to amplify cDNA fragments for digestive proteases from total RNA the hole body of the insects. The flanking sequences of the 5'- and 3'- end of the these genes were characterized by RACE-PCR. Sequence analysis showed that these genes contained complete ORF. The deduced amino acid sequences of these protease showed 62% identity to the serine protease of Creontiades dilutus, 58% to Lygus loneolaris trypsin-like serine proteinase , 54% to Triatonatoma infestans salivary trypsin. In the further study, to generate digestive protease, the DNA fragment coding for serine protease, trypsin-like serine protease were cloning into suttle vector pBACⅠ, and infected to Spodoptera frugiperda (sf9) insect cell. After that, we expect to carry out the proteolytic activity of these recombinant proteases. This is intended as a basis for future studies on the digestive protease in the insects.

The oxygen and nitrogen enriched activated carbons were obtained from modification of commercial activated carbon by using nitric acid, sodium hydroxide and urea. Zeta-potentials of modified activated carbons were investigated in relation to copper ion adsorption. The structural properties of modified activated carbons were not so much changed, but the zeta-potentials and isoelectric points were considerably changed. The zeta-potential of nitric acid modified activated carbon was the most negative than other activated carbons in the entire pH region, and the pHIEP was shifted from pH 4.8 to 2.6, resulted in the largest copper ion adsorption capacities compare with other activated carbons in the range of pH 3~6.5. In case of urea modified activated carbon, copper ion adsorption was larger than that of the as-received activated carbon from pH 2 to pH 6.5 even though the pHIEP was shifted to pH 6.0, it was due to the coordination process operated between nitrogen functional groups and copper ion. The adsorption capacity of copper ion was much influenced by zeta-potential and pHIEP of carbon adsorbent.

Ni-doped was prepared by the encapsulated induction melting and hot pressing, and its doping effects on the thermoelectric properties were investigated. Single phase was successfully obtained by the subsequent heat treatment at 773K for 24 hours. Nickel atoms acted as electron donors by substituting cobalt atoms. Thermoelectric properties were remarkably improved by the appropriate doping.

The encapsulated induction melting and hot pressing were employed to prepare Fe-doped skutterudites and their thermoelectric properties were investigated. Single phase was successfully obtained by the subsequent heat treatment at 773K for 24 hours. Iron atoms acted as electron acceptors by substituting cobalt atoms. Thermoelectric properties were remarkably improved by the appropriate doping. was found as an optimum composition for best thermoelectric properties in this work.

Compaction of asphalt pavement is one of the important processes to make good quality one. There are many laboratory-compaction methods to simulate field compaction, including Marshall compaction, Hveem compaction, gyratory compaction, and etc. The most common method used to determine the fundamental properties of asphalt mixture for design is Marshall method which is using impact energy. However, there is major difference between field compaction using kneading compaction and Marshall compaction using impact energy. Therefore, the gyratory compactor, which currently is the best to simulate the field compaction, was employed. The fundamental properties of asphalt specimen compacted by gyratory compactor and Marshall compactor were determined using laboratory test. From the tests, slag mixture with carbon black or pyrolyzed carbon black showed better performances, such as, in low susceptibility to temperature, high resistance against water and rutting, and high resilient modulus and indirect tensile strength.

Compaction of asphalt pavement is one of the important processes to make good quality one. There are many laboratory-compaction methods to simulate field compaction, including Marshall compaction, Hveem compaction, gyratory compaction, and etc. The most common method used to determine the fundamental properties of asphalt mixture for design is Marshall method which is using impact energy. However, there is major difference between field compaction using kneading compaction and Marshall compaction using impact energy. Therefore, the gyratory compactor, which currently is the best to simulate the field compaction, was employed. The fundamental properties of asphalt specimen compacted by gyratory compactor and Marshall compactor were determined using laboratory test. From the tests, slag mixture with carbon black or pyrolyzed carbon black showed better performances, such as, in low susceptibility to temperature, high resistance against water and rutting, and high resilient modulus and indirect tensile strength.