감마-오리자놀(γ-oryzanol)은 phytochemical의 한 종류로 항산화, 항염증, 항암, 항당뇨, 콜레스테롤 감소 등의 효능이 알려져 있다. 본 연구에서는 현미를 백미로 도정할 때 부산물로 발생하는 미강으로부터 생리활성물질 중의 하나인 γ-oryzanol 함량이 높은 추출조건에서의 분말을 제조하고, 이를 쌀국수에 첨가하여 항산화성이 개선된 국수를 제조하고자 하였다. 미강을 에탄올 농도 0, 20, 40, 80%의 에탄올로 추출한 결과 80% 에탄올로 추출한 추출분말에서 γ-oryzanol 함량이 가장 높았으며, DPPH 소거능과 ABTS 소거능이 가장 높았다. 미강추출분말의 γ-oryzanol의 함량을 높이기 위하여 Saccharomyces cerevisiae로 미강을 고상발효시킨 후 80% 에탄올로 추출하여 동결건조시킨 분말의 γ-oryzanol의 함량은 발효시키지 않은 미강을 추출분말보다 약 2.18배 증가하였으며, DPPH 소거능 및 ABTS 소거능, 단백질분해효능이 증가하였다. 오리자놀 함량이 높은 미강추출 물을 쌀가루에 첨가하여 쌀국수를 제조한 결과 쌀국수의 경도, 탄력성, 씸힘성, 점착성 등이 증가하였으며, 쌀국수의 γ-oryzanol 함량, DPPH 소거능, ABTS 소거능 등이 증가하여 항산화 활성이 개선되었음을 확인하였다.

The aim of this study was to produce a fermented rice bran extract with enhanced ferulic acid γ-oryzanol contents and high antioxidant activities. The ferulic acid content in the freeze-dried extract of rice bran treated with plantase PT enzyme, increased by 4.1-fold compared to that of untreated sample, the DPPH radical scavenging activity also increased by 1.5-fold and 1.2-fold, respectively. The γ-oryzanol content of the dried powder prepared by inoculating Apergillus oryzae BOT1869 onto steamed rice bran for solid-state fermentation followed by extraction with 80% ethanol, increased 2.3-fold compared to that in an 80% ethanol extract powder of raw materials. The ABTS scavenging activity also increased 1.5-fold. When the ferulic acid content-enhanced extract and the γ-oryzanol content-enhanced extract of rice bran were mixed and subjected to liquid fermentation with Lactiplantibacillus pentosus BOT406 and then freeze-dried, the ferulic acid content of the extract powder increased about 3.0 times compared to that of original extract powder. In addition, its γ-oryzanol content increased about 1.5 times, the DPPH radical scavenging activity increased 1.4 times, and the ABTS radical scavenging activity increased 1.6 times.

This paper addresses a scheduling problem aimed at minimizing makespan in a permutation flow shop with two machines and an inspection process that must be conducted at least once every certain number of outcomes from the first machine. A mathematical programming approach and a genetic algorithm, incorporating Johnson's rule and a specific mutation process, were developed to solve this problem. Johnson's rule was used to generate an initial population, while the mutation process ensured compliance with the inspection constraints. The results showed that within a computation time limit of 300 seconds, the mathematical programming approach often failed to provide optimal or feasible solutions, especially for larger job sets. For instance, when the process times of both machines were similar and the inspection time was longer, the mathematical programming approach failed to solve all 10 experiments with just 15 jobs and only had a 50% success rate for 100 jobs. In contrast, the proposed genetic algorithm solved all instances and delivered equal or superior results compared to the mathematical programming approach.

This study evaluated the immunogenicity of the Bacillus Calmette-Guérin (BCG) vaccine in a guinea pig model to refine preclinical assessment methods. 24 guinea pigs were divided into four groups for immunohistochemical, histopathological, and molecular analyses, including qRT-PCR and ELISA. The ELISA results revealed significant elevations in interleukin 2 (IL-2), interferon-gamma (IFN- ), and tuberculosis-specific antibodies in vaccinated guinea pigs, particularly γ notable after 6 weeks. Although lung cytokine levels remained unchanged, spleen gene expression showed significant differences in interleukin-17, interleukin-12, interleukin-1β, and C-X-C motif chemokine ligand 10 after 6 weeks. Immunohistochemistry revealed peak IL-2 expression at 8 weeks and significant IFN-γ and TNF-α expression at 6 weeks. This study confirmed the effectiveness of BCG vaccine in guinea pigs, providing crucial insights for future tuberculosis vaccine development and standardizing immune response indicators.

In this study, numerical analysis is conducted to investigate the flow characteristics of a turning type flood gate fishway with various design factors. The shapes of the fishway are circular and rectangular type. Baffles are installed to reduce the velocity in the fishway, and the gap and rotational arrangement of the baffles are set as design factors. To reduce the maximum velocity, a cavity-shaped break region is installed to examine the flow characteristics according to the presence of the break region. As a result, in the condition where the shape of the fishway is rectangular, the outlet average flow velocity is larger than that in the circular condition. The highest flow velocity occurs when the baffle is rotated in 90-degree arrangement. As the baffle gap increases, the average velocity increases. The cavity-shaped break region creates a recirculation zone in the fishway, and as a result, shows a decrease in the maximum velocity of up to 5.8%.

Graphene fiber is considered as a potential material for wearable applications owing to its lightness, flexibility, and high electrical conductivity. After the graphene oxide (GO) solution in the liquid crystal state is assembled into GO fiber through wet spinning, the reduced graphene oxide (rGO) fiber is obtained through a reduction process. In order to further improve the electrical conductivity, herein, we report N, P, and S doped rGO fibers through a facile vacuum diffusion process. The precursors of heteroatoms such as melamine, red phosphorus, and sulfur powders were used through a vacuum diffusion process. The resulting N, P, and S doped rGO fibers with atomic% of 6.52, 4.43 and 2.06% achieved the higher electrical conductivities compared to that of rGO fiber while preserving the fibrious morphology. In particular, N doped rGO fiber achieved the highest conductivity of 1.11 × 104 S m−1, which is 2.44 times greater than that of pristine rGO fiber. The heteroatom doping of rGO fiber through a vacuum diffusion process is facile to improve the electrical conductivity while maintaining the original structure.

Resistance to extended-spectrum cephalosporin in Enterobacteriaceae is increasingly prevalent in South Korea. This study aims to explore the distribution of AmpC genes in Proteus mirabilis isolated from stray and hospital-admitted companion animals in South Korea. AmpC β-lactamases hold clinical significance due to its potential to facilitate antimicrobial resistance to cefoxitin, cefazolin, and most penicillins. A total of 163 bacterial isolates belonging to the Enterobacteriaceae family were collected from dogs (n = 158) and cats (n=5). Of them, 134 isolates were from hospital-admitted animals, while 29 isolates from stray animals. Boronic acid tests and antimicrobial susceptibility tests were conducted for an initial screening to detect AmpC β-lactamase resistant P. mirabilis. Gene-specific PCRs were conducted to identify the type of AmpC genes, which include six groups (MOXM, CITM, DHAM, ACCM, EBCM, and FOXM), in the resistant isolates. The boronic acid disk tests revealed 45 (27.6%) positive isolates out of 163 isolates tested. Of these 45 isolates, six were determined to harbor the EBCM gene, 13 for CITM, one for FOXM, and one for DHAM by single detection PCR. No isolates carried for ACCM or MOXM. Thus, a total of 21 out of 163 isolates (12.9%) were demonstrated to possess AmpC genes. No isolates contain more than one group of AmpC gene family. A significantly higher percentage of P. mirabilis was found to possess AmpC genes compared to past studies. Therefore, the increasing trend in antimicrobial resistance in P. mirabilis indicates a dire need to monitor antimicrobial prescription in the veterinary field.

농촌진흥청 국립원예특작과학원에서는 2017년에 절화 수명이 길고 수량이 많은 연한 핑크색의 스프레이 장미 ‘Pink Shine’ 을 육성하였다. 모본은 ‘Fire Flash’로 붉은 복색의 스프레이 장미이며, 부본은 ‘Pink Charm’으로 핑크색이며 흰가루병에 강하다. 이 두 품종을 2012년 인공교배하여 이듬해인 2013년 1월에 파종, 9cm 포트 묘에 정식하여 관능 평가 실시 후 도태시켜 39개체 의 실생을 얻었다. 이후 화형, 화색, 꽃잎 수, 절화수량, 병 저항성 등을 고려하여 2015년까지 5개체를 선발하여 유사 품종인 ‘Missha’를 대조로 하여 2017년까지 3차에 걸친 특성 검정을 실시하였다. 그 결과 가장 우수한 ‘원교 D1-325’를 최종선발하여 ‘Pink Shine’으로 명명 후 2018년 3월 22일 품종보호출원(제 2018-212호)하여 2019년 6월 21일에 품종보호권(제7786호)이 등록되었다. 화색은 연한 핑크색(RHS, R36D)이며 잎의 색은 녹색(RHS, G137A)으로 대조 품종 ‘Missha’와 동일하였다. 꽃잎 수는 67.8개, 화폭 5.4cm, 화고 3.2cm로 ‘Missha’보다 컸으며 평방미터당 연간 절화수량은 131본, 절화수명은 15.3일로 ‘Missha’ 보다 우수하였다.

상선에 당직사관으로 승선하기 위한 해기사는 승선 전 해기사로서 갖추어야 할 적절한 자격요건을 국제규정에 맞게 충족시켜야 한다. 해사영어 활용 능력은 이와 관련한 중요한 요소 중 하나이다. 해기 교육 과정에 입교한 학생들은 교내 수업을 통해 선박과 관련된 영 문 서류, 검사 문서 그리고 사고 보고서에 이르기까지 다양한 상황에 대한 해사영어활용능력을 습득하게 되며 이를 통해 일반 영어뿐만이 아닌 실무에서 사용되는 해사분야 전문 용어 및 특수 문장까지 학습하게 된다. 하지만 선박 및 해운산업의 특수성으로 인해 해사영어를 포함한 해기 지식이 전달되고 또 평가되는 데에는 많은 제약이 존재한다. 해당 연구는 항시 접속 가능한 해사영어 학습 및 시험 플랫폼의 활용을 기반으로 학생들의 학습 결과를 측정하고 이를 통한 교육 효과를 조사하였다. 실험은 승선실습 과정에 참여한 실습항해사를 두 집단으로 나누어 진행되었다. 한 집단은 해사영어 학습 및 평가 플랫폼을 활용하였고 다른 한 집단은 해사영어 학습 및 평가 플랫폼을 활용하지 않았다. 6주의 실험을 통한 최종 시험 결과 두 집단 간 해사영어 시험성적에 유의한 차이가 발생하였으며 이를 통해 해사영어 학습 및 평가 플랫폼의 활용이 해사영어 시험 점수에 유의미한 점수 차이를 가져오는 것을 확인하였다. 또한 학생들의 개인 학습시간을 조사하여 그 교 육 효과를 정량화하였으며 나아가 추후 연구에 대한 개선방향을 제시하고자 하였다. 해당 연구는 해사 교육 분야에 다양하게 적용하여 활 용할 수 있는 학습 플랫폼 활용 효능을 조사하였으며 향후 해사영어 교육 외에 그 범위를 넓혀 활용될 수 있을 것으로 사료된다.