본 연구는 1979년부터 2022년까지 발표된 문신 및 반영구화장 관련 논문 198편을 대상으로 발표시기, 주제, 연구방법에 따라 체계적 문헌고찰을 수행하였다. 시기별로는 제1기(1979년∼1994년), 제2 기(1995년∼2005년), 제3기(2006년∼2012년), 제4기(2013년∼2022년)로 나누었다. 제1기에는 보건의료적 측면의 연구가 주를 이루던 시기이며 제2기에는 예술, 문화 등 다양한 측면의 연구들이 나타나기 시작하였 다. 제3기는 반영구화장에 관한 연구가 증가한 것이 특징이며 제4기는 문신 및 반영구화장의 연구가 급증 하였다. 연구주제별로는 보건·의료, 미용, 패션·예술, 사회·문화, 법·제도로 나누어 분석하였다. 그 결과 소비 자의 선호도와 인식도 등을 파악하기 위한 미용적 연구가 가장 활발하게 이루어졌으며, 보건의료적 연구가 두 번째로 많았다. 최근 법제화의 필요성이 높아짐에 따라 법·제도와 관련한 연구들이 급증하였으며, 문화, 역사, 패션 등에 관한 연구도 이루어졌다. 연구방법별로는 조사연구 방법을 가장 많이 활용하였으며 문헌 연구, 사례연구 등 다양한 방법도 이용되었다. 본 연구로 향후 후속 연구가 더욱 촉진될 것이며 관련 산업 의 발전에도 기여할 것으로 기대한다.

This study monitored temperature using electronic sensors and developed a prediction model for compost maturity. The experiment used swine manure in a mechanical composting facility equipped with a screw-type agitator, and the composting process was conducted for 60 d during the summer season in South Korea. Four electronic temperature sensors were installed on the inner wall between the compost piles on Days 7, 14, 21, and 28 for daily temperature monitoring. Compost samples were collected daily for 60 d, and compost maturity was analyzed using the Solvita method. Multiple comparisons, correlations, and modeling were performed using the stat package in R software. The average compost pile temperatures was 39.1±3.9, 36.4±4.3, 31.3±4.5, and 35.4±8.1 on days 7, 14, 21, and 28, respectively, after composting. The average compost maturity according to the composting date was 3.61±0.60, 4.13±0.59, 4.26±0.47, and 4.32 ±0.56 on days 7, 14, 21, and 28, respectively. A significant negative correlation was observed between the compost composting periods (seven, 14, 21, and 28 d) and the temperature of all compost piles (p<0.05), where the correlation coefficients were -0.329, -0.382, -0.507, and -0.634, respectively. A significant positive correlation was observed between the compost composting periods (seven, 14, 21, and 28 d) and the maturity of the compost (p<0.05), where the correlation coefficients were 0.410, 0.550, 0.727, and 0.840, respectively. The model for predicting the maturation of the 14 d average compost pile according to the compost composting period and the average temperature for 14 d was y=0.026 x d – 0.021 x mt.x_14 d (mean temperature for 14 d) + 4.336 (R2=0.7612, p<0.001). This study can be considered a basic reference for predicting compost maturity by the proposed model using electronic temperature sensors.

본 논문은 1939-1941년까지 듀크 엘링턴(Duke Eliington) 밴드에서 베이스를 담당하던 지미 블 랜튼(Jimmy Blanton)과 그의 연주를 영향 받은 거장 베이스 연주자 레이 브라운(Ray brown)의 즉 흥연주 비교 논문이다. 이 연구의 목적은 재즈 더블 베이스의 역할의 다양성과 라인의 변화가 어 떻게 시작되었고 발전되어 왔는지를 인지할 수 있도록 하고, 그것으로 인해 연주자들이 다양한 음악적인 접근과 시도로 음악적의 넓은 접근을 할 수 기여할 수 있도록 하는 데 목적이 있다. 지미 블랜튼은 처음으로 모던한 재즈 베이스 연주 스타일을 선보였던 베이스 연주자이며 레이 브라운은 모드 스케일의 사용과 잦은 반음사용이 특징인 비밥 시대를 대표하는 베이스 연주자였 다. 본 논문은 먼저 1930년대 스윙시대의 음악적 배경, 지미 블랜튼과 레이 브라운의 생애와 연주 스타일을 살펴보았고, 두 연주자가 듀크 엘링턴과 연주한 공통적인 연주곡인 <Pitter Panther Patter> 을 각 마디별로 나타난 특징을 분석하여 비교하였다. 그 결과 지미 블랜튼과 레이 브라운 의 즉흥연주 공통점은 잦은 코드톤의 사용과 다양한 스케일의 사용, 패턴의 사용, 선행음의 사용, 리듬의 변화를 통한 다이나믹 연출 등이 분석되었다. 또한 레이 브라운의 연주에서 나타난 특징인 특징음을 코드톤과 연결하여 사용하여 마이너적인 느낌의 연주를 하는 것이 지미 블랜튼의 연주에 서 시작되었다는 결론이 도출되었다. 그러나 <Pitter Panther Patter>곡의 특성상 패턴적인 멜로 디와 짧은 솔로 브레이크로 좀 더 다양한 특징이 분석되지 못한 것이 본 논문의 한계점이라 할 수 있다.

최근 딥러닝 기술 가운데 이미지데이타 분석에 뛰어난 성능을 보이는 합성곱신경망 기술의 발전은 이미지 분석 영역에서 다양한 가능성을 제시하고 있다. 관광객이 게시한 사진을 딥러닝 기술을 이용하여 분류하기 위해서는 관광사진에 대한 분류와 목적에 맞는 딥러닝 모델의 훈련작업이 필수적으로 선행되어야 한다. 본 연구에서는 관광객이 플리커에 게시한 사진을 효율적으로 분류하기 위해 관광목적으로 사진이 어떻게 분류되어야 하는지 관광목적 사진분류 체계를 개발하고자 하였다. 관광목적 사진 분류 카테고리 개발을 위해 문헌분석, 웹사이트 분석, 관광객이 게시한 약 38,000장 사진의 검토과정을 거쳐 사진 분류 카테고리를 개발하였으며, 약 8400장의 사진을 개발된 카테고리에 맞춰 분류해 봄으로써 개발된 카테고리의 검증과정을 거쳤다. 이 과정을 거쳐 최종으로 제안된 카테고리는 13개 대분류, 64개 중분류, 164개의 세분류 체계를 갖으며, 본 연구 결과는 향후 관광목적 사진을 딥러닝 모델을 이용하여 분류하고자 할 때 기초자료로 활용될 것으로 기대된다.

The Wolbachia bacterium, one of the most prevalent endosymbiotic bacteria, is known to induce reproductive anomalies in various invertebrate taxa. We investigated Wolbachia infection frequency in 203 Coleopteran insects collected from Korea. Among them 26 species (12.8%) across families proved to harbor Wolbachia. The phylogenetic trees of two Wolbachia specific genes imply that there have been complex horizontal gene transfers and recombination events within and between divergent Wolbachia subgroups. Interestingly, we also find that the infection frequency is noticeably low and incongruent at the family level when compared to other literature cases of Wolbachia infection in beetle species.

에메리개미는 여왕개미와 수개미가 유전적으로 복제되어 번식한다고 알려져 있으며, 여왕개미의 날개형태가 장시형과 단시형으로 나타난다. 장시형은 정상적인 날개형태이고, 이보다 짧은 날개형태는 단시형이라고 한다. 장시형과 단시형 모두 한 종으로 취급되지만, 두 가지 점에서 종 지위에 대한 조사가 필요하다. 첫째, 자연 상태에서는 두 날개형이 함께 발견되지 않고, 둘째, 날개형이 육안으로 뚜렷하게 구분된다. 또한 복제되어 번식한 여왕개미가 단수체인지 배수체인지 조사가 필요하다. 따라서 우리는 본 연구에서 에메리개미 유전체 크기를 추정하여 두 날개형은 동종이며, 여왕개미는 배수체임을 확인하였다.

Haplodiploid sex determination is prevalent in nature comprising about 25% of higher taxa. Due to its uniqueness, selfish entity may relatively easily slip in the system. Here, I will introduce 3 different types of selfish entities. First, some microorganisms are known to distort sex ratio in favor of their own prosperity. Second, a genetic fragment, called paternal sex ratio chromosome, is classified as the extremely selfish element so far in haplodiploid. Finally, haploid genome itself can evolve selfishness. The first two result in skewed sex ratio, and the last in completely new genetic relatedness. I will also discuss their evolutionary significance and potential utilization in controlling pest populations.

Ants have been highly successful throughout the evolution: spreading over all continents except Antarctica and constituting 15~25% of total biomass on Earth. Leafcutter ant (Atta cephalotes) inarguably represents the most sophisticated social structure among numerous ant species. To understand the molecular mechanism of the caste determination and divergence, we extracted RNA from the head and DNA from the body of single workers based on their size and analyzed them by next-generation sequencing technique. The preliminary results demonstrate power of the approach to uncover underlying biology of leafcutter ants, which was not feasible before the advent of NGS technique using conventional model species.

All aspects of hosts’ life are affected not only by symbiotic bacterial community but also endosymbionts, such as physiological function, mating preference and fitness. Wolbachia, one of the most common endosymbionts, known as a manipulator of host sex ratio, may also have a huge effects on other symbionts. Here, we assessed the effects of Wolbachia on the diversity and structure of other symbiotic microbial communities in six ant species by using next-generation sequencing. Our results show that the diversity of the symbiotic microbial communities between Wolbachia-positive species and negative species is not different. However, according to the Wolbachia infection, dominant symbiotic microbes of each host species were different as well as the composition of the overall symbiotic microbial community. Thus, this study suggests that, as Wolbachia have an important role in shaping microbiome of hosts, endosymbiont infection status deserves special consideration in studying microbiome.

외래생물인 아메리카동애등에는 뛰어난 유기물분해능으로 주목받아왔다. 이에 우리는 한국내 분포상과 유전적 다양성을 알아보기 위해 전국적으로 채집하여 미토콘드리아의 사이토크롬 C 산화효소 1 (CO1) 유전자를 이용해 집단유전학분석을 실시하였다. 그 결과 이 곤충은 한국에 성공적으로 정착한 것으로 보이며 10개의 미토콘드리아 유전자형이 있었고 개체군 간 분화가 뚜렷했다. 이 결과는 국내정착한 아메리카동애등에가 특정 유전자형의 모계만이 도입된 후 지역 간 분산이 제한적임을 나타내는 것으로, 이는 이들의 단거리 비행 경향에 기인한 것으로 추정된다.

By definition, endosymbionts reside in the hosts' cells and are maternally inheritted to offspring as the mitochondriondoes. Recent progresses reveal that some endosymbionts have evolved their manipulabilities of hosts' reproduction. Inthis nature, once an endosymbiont shares its host with others, their association may last long unless their evolutionaryinterest or hosts' fitness is undermined.We tested whether the association existed among the endosymbionts; Wolbachia, Spiroplasma, Cardinium and Rickettsia.The results show that there are some multiple infection cases but there is no obvious pattern in their association.

Vollenhovia emeryi (Formicidae: Myrmicinae) is divided into two morphs based on the wing length of the queen caste: the long-winged with a normal wing and the short-winged with the aberrant short wing. The phylogenetic analysis shows that the short-winged is derived from the long-winged. In Korea, only the long-winged morph is infected with Wolbachia while the short-winged is devoid of the bacterium suggesting that the short-winged evolved the resistance to the bacterial infection. Intriguingly, some Japanese short-winged colonies proved to still be infected with the bacterium. We hypothesized that the infected Japanese short-winged is the intermediate form in the process to complete cure. However, the data of the MLST and the measurement of Wolbachia density did not support our hypothesis. Our further experiment using microsatellite markers shows that the infected Japanese short-winged shows the similar genetic background to the long-winged. In this presentation we will discuss the potential resistance evolution in the Korean short-winged and future research direction at the genome level.

Salmonellosis is one of the most common food-borne diseases in both humans and animals. The recovery of Salmonella from fecal and environmental samples by bacteriological assays takes several days. Polymerase chain reaction (PCR) has become an important technique for the rapid detection of Salmonella in a variety of samples, including feces. For rapid identification of Salmonella by PCR, 1 mL of enrichment culture was harvested after overnight incubation and DNA was extracted by heat lysis. To investigate the optimal conditions for rapid PCR detection of Salmonella, three different primer sets and three different enrichment media were used on a panel of Salmonella strains and a panel of non-Salmonella strains. The results showed that selenite cysteine enrichment broth and a primer set designed for the invA gene provided the most specific and rapid detection of Salmonella by PCR after the enrichment step.

The phylogenetic analysis the queen polymorphic Vollenhovia emeryi ant reveals derivation of the Wolbachia- free short-winged from the Wolbachia-infected long-winged. However, intriguingly, some Japanese short-winged colonies harbor Wolbachia. Wolbachia specific bacteriophage (WO) is also detected in more than half of the infected colonies with no clear distribution pattern across the host insect lineage. We hypothesized that 1) the infected Japanese short-winged is in the intermediate stage to complete loss of Wolbachia and 2) the phage invaded the host after the host insect diverged. To test the hypotheses, we studied the strain diversity using the multi-locus sequence typing (MLST) of five ant colonies; three longwinged colonies from Korea and one long-winged colony and one short-winged colony from Japan. Both Korean and Japanese V. emeryi colonies show unexpectedly high level of Wolbachia strain diversity. However, the diversity is not significantly different between the long-winged and the short-winged against our first hypothesis. Phylogenies of Wolbachia show Korean strains and Japanese strains are largely monophyletic indicating prior infection before the host divergence. The strain diversity of the phage is also surprisingly high. Phylogenies of orf2 and orf7 genes are incongruent to that of Wolbachia and geographically distinct. This indicates that the phage is spatially static and the current infection pattern may be the consequence of local repeated gain and loss of the phage.

The ant species, Vollenhovia emeryi Wheeler (Hymenoptera: Myrmicinae) is endemic in East Asia and has invaded into North America. In this species, the queen caste shows polymorphism in its wing morphology; long-winged queen and short-winged queen, and two morphs are thought not to coexist in nature. This research is conducted to 1) deduce the phylogeographical structure of the two wing morphs in South Korea and to trace the distribution pattern from East Asia to North America, and 2) investigate the Wolbachia and WO phage infection frequency of the species. Either individuals or colonies of V. emeryi were collected from 80 locations, encompassing 68 locations in South Korea, 11 in Japan, and one in USA. Among the collected samples in South Korea, the long-winged morph is dominant and considered as the ancestral type, while the short-winged morph is very rare and derived character. The origin of the US population is neither Korea nor Japan at least in this study. However, we do not exclude the possibility that its origin is the other parts of Japan or the other countries. All of the long-winged morph are infected with Wolbachia, while the short-winged seems to be geographically partially infected. It suggests the possibility that the short wing trait is linked with the evolution of resistance to Wolbachia infection. Bacteriophage WO infection status has no correlation with host insect lineage.

In Hymenoptera, thanks to its haplodiploid sex determination, arrhenotokous parasitoid females are able to control offspring sex ratio by determining whether to use sperm or not at the time of oviposition. However, the offspring sex ratio may also be influenced by the mating behavior of a male. When a male searches females, he will have more chances to encounter with other males in a high male density population than low male density population. If the male is competitive, the male can find females faster and may have longer copulation time, and eventually transfer more sperm than other males. In this study, we investigated mate searching time, copulation duration and offspring sex ratio as a function of the male density. Although the results show difference between searching time and copulation duration, they are not statistically significant. We will discuss the statistical consequence, male density dependent offspring sex ratio and its evolutionary implications.

In Korea, twelve species in genus Myrmica have been described. Some of them are morphologically similar and this makes their identification difficult. For this reason, we collected several Myrmica species in question and inferred their phylogenetic relationship using the 418bp partial COI (cytochrome C oxidase 1) region from a total of 33 individuals. We found that the CO1 haplotypes are effectively grouped into three clusters that match well to their external morphological characters. Although this three species could be distinguished by the only small part of the COI region, the two individual sample of the M. kotokuii and one sample of the M. carinata are included in the M. kurokii group. The results indicate that the morphological identification could be obscure in the three species and it requires a close examination for this phenomenon.

Wolbachia is one of the most common endosymbionts best known to induce several reproductive alterations in its insect hosts. In some cases, the insect hosts harbor more than two strains of the bacterium. The Vollenhovia emeryi ant lives in dead trees and is morphologically subdivided into the long-winged and the short-winged. Interestingly the short-winged morph is free of Wolbachia, but only the long-winged morph is multiple- infected with the Wolbachia bacterium. We sampled four populations of the long-winged morph in Korea and performed pyrosequencing in Multilocus Sequence Typing (MLST), to determine the bacterial strain diversity. Six different gene regions (coxA, fbpA, ftsZ, gatB, hcpA and wsp gene) were targeted and amplified. However, the result shows that diversity of haplotypes is very high. The pyrosequencing approach in MLST, a new method of discriminating Wolbachia strains, is promising to effectively detect multiple infections and rare haplotypes.