In apple orchards under different farming practices (organic and conventional), arthropod community structure was investigated. Arthropods were collected with pitfall traps, band traps, yellow sticky traps and pheromone traps from May to December, 2012 in Danyang-gun, Chungcheongbuk-do, Korea. Biodiversity was analyzed with species richness and Shannon diversity index (H΄). Sampled arthropods were identified 37 families in 14 orders from 21,618 individuals in organic orchard-1 and 33 families in 10 orders from 11,318 individuals in organic orchard-2 and 35 families in 13 orders from 27,711 individuals in conventional orchard. Species richness was relatively highest in organic orchard-1, intermediate in conventional orchard and lowest in organic orchard-2. In ecologically functional group, abundance of pests was higher in conventional orchard than in organic orchards due to the high occurrence of Phyllonorycte ringoniella (Gracillariidae) and Lyonetia prunifoliella (Lyonetiidae). Organic orchards showed higher abundance, species richness and species diversity of epigeic predators and those of parasitoids above ground than conventional orchard. Abundance of detritivores, mainly collembola, were higher in organic orchard than in conventional orchard.

Paromius exiguus (Distant, 1883) is a serious insect pest in rice field adjacent to reclaimed land. They suck young ear of rice causing empty head of grain or pecky rice grain. During 2010 to 2012, we conducted to investigate the host-plant change of P. exiguus in Siwha reclaimed land, Hwaseong-si. In 2012, we also studied dispersal ability of P. exiguus from reclaimed land to adjacent rice fields. In early May, the adults aggregated on the grain parts of cogon grass (Imperata cylindrica) (Poales: Poaceae) after overwintering, and laid their eggs and nymphs developed into adults on the same host plants from middle May to late June. Second generation was observed at wood small-reed (Calamagrostis epigeios) (Poales: Poaceae) from late June to late July. At shooting stage of rice, adults moved to adjacent rice fields and third generation might be started. Although dispersal ability of adult of P. exiguus penetrated about 10 km into inland areas of Hwaseong-si, number of individuals of P. exiguus population was significantly declined with increasing distance from reclaimed land (Pearson’s correlation coefficient, r=-0.7357, p<0.0001). Unfortunately, further studies on population dynamics of P. exiguus in rice fields could not investigated, because pesticides were widely sprayed by aerial application in 14 August, 2012. Nevertheless, this results will be a useful information for management and control of P. exiguus in rice fields adjacent to reclaimed land.

Food web of main arthorpods in 3 differently managed rice fields (low input, duck, apple snail farming) was investigated in Hongseong area, Chungcheongnam-do, Korea. Main arthropods were selected by their abundance during the rice growing season in 2010. Food web was analyzed with stable isotope, δ15N and δ13C according to rice growth satges (seedling, tillering, booting, blooming/heading, and ripening stage). Rates of δ15N and δ13C in soil (F=5.72; df=2, 6; P=0.0407, F=40.86; df=2, 6; P=0.0003, respectively) and δ13C in rice (F=6.46; df=2, 6; P=0.0319) were statistically different. Rate of δ13C in Lissorhoptrus oryzophilus (F=9.50; df=2, 6; P=0.0138), Pirata subpiraticus (F=11.00; df=2, 6; P=0.0098), and Pachygnatha clercki (F=6.33; df=2, 6; P=0.0333) were statistically different among farming methods. Stable isotope structures in food web were slightly different among 3 farming methods seasonally. Rates of δ15N and δ13C of rice pests such as Nephotettix cincticeps, Nilaparvata lugens, Laodelphax striatellus, and Sogatella furcifera were related to rice plant. Those of Tomoceridae sp. and Chironomidae sp., however, were not related to rice plant. Spiders as main natural enemy group were ranked on the top of food web and were thought to have life cycle based on the rice field from stable isotope structure.

We investigated the spatial distribution of ground beetle species from the edge of secondary forests in Hwaseong-si, Gyeonggi-do. Nine secondary forests were selected, and 81 pitfall traps for collecting ground beetles were placed along forest interior―forest edge―forest exterior gradients during 14 June to 9 November, 2011. A total of 32 species belonging to 13 genera of 8 subfamilies were identified from 3710 collected ground beetles. Ten dominant species were selected for analysis. Five species such as Chlaenius micans (ANOVA, with Tukey's test, F2, 78=3.11, P=0.0502), Chlaenius ocreatus (F2, 78=2.76, P=0.0692), Dolichus halensis halensis (F2, 78=9.80, P=0.0002), Harpalus eous (F2, 78=2.73, P=0.0712), and Harpalus tridens (F2, 78=6.74, P=0.0020) were abundant at forest exterior. Three species such as Synuchus cycloderus (F2, 78=7.91, P=0.0007), Synuchus nitidus (F2, 78=9.72, P=0.0002), and Synuchus sp.1 (F2, 78=4.50, P=0.0142) were abundant at forest edge and forest interior. Coptolabrus smaragdinus branickii (F2, 78=3.24, P=0.0444) was abundant at forest edge and exterior. Finally, Chlaenius naeviger (F2, 78=1.39, P=0.2542) showed a wide distribution in this study. This study showed a significant edge effect on dominant ground beetles. Many environmental variables and prey items may be important factors for the spatial pattern of ground beetles. Because forest edges are generally important for many predaceous arthropods including ground beetles, the conservation and management of forest edges are important for maintaining biodiversity and ecosystem functions.

Community structure of ground beetles in Seokmun- and Siwha-reclaimed lands was investigated with pitfall traps from 21 May to 1 November, 2010. Survey areas were divided by the status of reclamation, vegetation, and flooding frequency. Status of the Seokmun-reclaimed land in Chungcheongnam-do is the progress of conversion to rice paddy after completion, and then dominant plant is Secale cereals. For the Siwha reclaimed land in Gyeonggi-do, reclamation is ongoing, and thus Imperata cylindrica and Calamagrostis pigeios are dominant plants. Flooding was more frequent in Seokmun than in Siwha as 4 times and none, respectively. A total of 3,352 epigeic insects of 9 orders and 22,981 epigeic insects of 9 orders were collected from the Seokmun- and Siwha-reclaimed land, respectively. Collembola was the most abundant in each site. In ground beetle assemblage, 11 species belonging to 7 subfamilies Seokmun and 8 species belonging to 5 subfamilies in Siwha were identified from 183 and 101 collected ground beetles. Chlaenius pallipes (107 individuals, 58.5%) and Pheropsophus javanus (52 individuals, 28.4%) was dominant in Seokmun, while Harplus jureceki (37 individuals, 36.6%), Synuchus arcuaticollis (17 individuals, 16.8%), and Dolichus halensis halensis (17 individuals, 16.8%) was dominant in Siwha. The Shannon's index value was 1.147 and 1.671 in Seokmun and Siwha, respectively. Similarity by the Sørensen index between Seokmun and Siwha was 0.3158. In conclusion, community structure and seasonality of ground beetles were significantly different between two reclaimed lands, and this difference appears to be due to their different habitat conditions such as flooding frequency and progress of reclamation.

Field studies were conducted to assess potential effects of transgenic rice expressing Bacillus thuringiensis (Bt) CryIAc1 protein, which is highly effective the rice leafroller, Cnaphalocrocis medinalis, on the rice insect community in 2007 and 2008. Insects were sampled in non-Bt and Bt rice plots with a sweep net and a suction devise. A total of 64,256 individuals in 45 families and 11 orders (30,860 individuals in 43 families and 11 orders in the Bt-rice plots, and 33,396 individuals in 40 families and 11 order in the non-Bt rice plots) were captured and grouped by ecological functional guilds. Species diversity and richness were not significantly different between Bt-rice and non-Bt rice plots. The seasonal pattern of the insect community and the seasonal population fluctuation of insects were very similar between two plots. Collectively, the rice insect community was not influenced by the Bt-rice in the present study.

This study was conducted to investigate the altitudinal distribution of the ground beetle community in a mountain area during 2007 to 2009, and to examine what habitat characters are most relevant to the distribution of mountain ground beetle species, and finally to identify potential biological indicators for monitoring in forest. The study area was Mt. Sobaeksan National Park (1439 m elevation), in Punggi-up, Gyeongsangbuk-do, Korea. Ground beetles were collected by pitfall traps, which were installed along the altitudinal gradient 437, 757, 1100 and 1420 m. In total, 3,259 individuals were identified as 32 species in 18 genera. The dominant species were Synuchus cycloderus (29.4%), Eucarabus sternbergi sobaeksanensis (15.4%) and Pterostichus orientalis (9.7%) in order. Overall, the ground beetle community structure was different along the altitude (similarity 65.2%) by NMDS ordination, but also were clustered into 2 groups, 437 and 757 m, and 1100 and 1420 m (similarity 57.9%). Therefore, the ground beetle assemblage could provide basic information for forest management. In this study, some ground beetle species were suggested to potential biological indicators along altitudinal gradient.

Insulin-like growth factor II (IGF2) and H19 genes are mutually imprinted genes which may be responsible for abnormalities in the cloned fetuses and offspring. This study was performed to identify putative differentially methylated regions (DMRs) of porcine H19 locus and to explore its genomic imprinting in in vitro fertilized (IVF) and somatic cell nuclear transferred (SCNT) embryos. Based on mice genomic data, we identified DMRs on H19 and found porcine H19 DMRs that included three CTCF binding sites. Methylation patterns in IVF and SCNT embryos at the 2-, 4-, 8～16-cells and blastocyst stages were analyzed by BS (Bisulfite Sequencing)-PCR. The CpGs in CTCF1 was significantly unmethylated in the 2-cell stage IVF embryos. However, the 4- (29.1%) and 8～16-cell (68.2%) and blastocyst (48.2%) stages showed higher methylation levels (p<0.01). On the other hand, SCNT embryos were unmethylayted (0～2%) at all stages of development. The CpGs in CTCF2 showed almost unmethylation levels at the 2-, 4- and 8～16-cell and blastocyst stages of development in both IVF (0～14.1%) and SCNT (0～6.4%) embryos. At all stages of development, CTCF3 was unmethylated in IVF (0～17.3%) and SCNT (0～1.2%) embryos except at the blastocyst stage (54.5%) of IVF embryos. In conclusion, porcine SCNT embryos showed an aberrant methylation pattern comprised to IVF embryos. Therefore, we suggest that the aberrant methylation pattern of H19 loci may be a reason for increased abnormal fetus after embryo transfer of porcine SCNT embryos.

본 연구는 중년여성을 대상으로 운동수행기간 별로 구분한 에어로빅운동군과 좌식 생활자의 신체계측, 식이섭취조사, 체력검사, 혈청지질검사를 하였으며 혈청지질과 각 인자와의 상관관계 및 체력과 신체계측과의 상관관계를 산출하였고 그 결과 및 결론은 다음과 같다. 1) 체력검사의 결과 : 조사대사장의 최고, 최저 혈압측정치는 좌식생활자에 비해 운동군이 낮았고 맥박은 운동수행기간이 길수록 그 수치가 낮아졌다. 맥압은 45이상인 자가 17.1%로 심장질환에 주의가 필요하였다. 근지구력과 점프력은 대상자 모두 정상범위이며 A군이 우세하였다. 유연성에서 C군은 미달이고 A군 정상범위이었으며 상반신근력은 A, C군 모두 정상범위에 미치기 못하였으며 평형성은 운동수행기간이 긴 AⅢ군이 가장 우세하였다. 2) 식이섭취 조사 결과 : 조사대상자들의 1일 섭취칼로리는 권장량과 비교하여 큰 차이를 나타내지 않았지만 단백질 섭취는 권장량에 따라 월등히 많이 섭취하였다 칼슘의 1일 섭취량은 권장량 600㎎에 비해 모두 높았고 철분은 운동기간이 가장 긴 AⅢ군이 (16.1+43㎎으로) 권장량 18㎎/day에 비해 섭취미달이었다. 식이중 콜레스테롤의 1일 섭취량은 한국정상인 1일 섭취량인 200∼300㎎과 큰 차이가 없었으며 식이 지질중 P/S률은 AⅡ군만 1.0보다 낮고 모두 정상비율이었다. 3) 식이와 혈청지질과의 상관관계 조사결과 :조사대상자들의 힐청지질중 중성지질과 단백질 섭취와는 r=0.42(p<0.05)로 유의전인 양의 상관으로 나타났다. 이는 과잉의 단백질 섭취의 결과로 중성지질로 전환됨을 일 수 있었다. 혈청 TC와 식이 TC와는 r=0.21이나 유의적 상관관계를 보이지 않았다. 이는 식이로 섭취된 TC가 혈청 TC에 큰 영향을 미치지 않았다고 사려된다. 4) 체력 및 체위와 혈청지질과의 상관관계 조사결과 : 혈청 TG, TC, LDL-C와 체력과는 음의 관계이며 HDL-C, HDL-C/TC와 체력과는 양의 관계이었다. 운동에 의한 체중 감량이 클수록 혈청 HDL-C농도가 높아졌으며 체력이 향상되면 혈청지질도 개선될 수 있음을 알 수 있었다. 최고혈압과 TC는 r=0.49(p<0.05), LDS-C와는 r=0.47(p<0.05)로 유의적인 양의 상관으로 혈압이 높으면 혈청 TC, LDL-C도 높게 나타났다. 5) 신체계측과 체력과의 상관관계 조사결과 : 체중감량과 윗몸일으키기는 r=0.71 (p< 0.001), 팔굽혀펴기와는 r=0.54(p<0.001)로 유의적인 양의 상관관계를 나타냈다. 즉 운동에 의한 체중감량은 근지구력을 높힐수 있으며 운동후 체중과 서서위로뛰기는 r=0.49(p<0.()5)로 유의적 양의상관을 나타냈다. 즉 aerobic운동에 의해 점프력이 향상될 수 있음을 알 수 있었다. 6) 식생활에 대한 설문분석 결과 :조사대상자들의 체지방율이 30%이상인사가 14.8%로 운동을 하게된 동기가 비만해소보다는 건강증진에 목적이 있었으며 식사형태는 육, 채식 혼합식이 71.9%로 균형된 식사의식이 높았다. 그러나 적당히 당질식품과 당음료를 섭취한다가 53.6%로서 과잉의 당질섭취로 중성지방 전환의 우려를 고려해야 할 것이며 83.2%가 카페인 함유 음료를 음용하므로 혈압상승 및 철분흡수의 방해가 우려된다.

This study was attempted to establish the basic data for effective utilization of the dried radish and sweet potato. The rehydration characteristic was carried out from these dried root vegetables in various conditions. The following results were obtained. The rehydration value was increased in glycine solution, whereas it decreased in lactic acid solution. Also the vacuum freezing was higher than that in hot air drying, and it was higher the slow freezing than in the quick freezing. The rehydration rate and the rehydration surface area curve were composed of three stages, and these stages were corresponded to each other. At the range of initial immersion to 2min. , the largest rehydration rate was showed. The activation energy obtained from the Arrhenius plot of the rehydration rate constant(K) were 3.8×10 exp (3)㎈/g ㏖ and 3.7×1010 exp (3)㎈/g ㏖ for dried radish and sweet potato, respectively.