Metarhizium은 대표적인 곤충병원성 진균 중 하나로, 종에 따라 매우 다양한 곤충에게 병원성을 일으키는 폭 넓은 기주범위를 형성한다. 이들이 주로 생성하는 것으로 알려진 destruxins (DTXs)이라는 2차 대사산물은 살충 활성 뿐만 아니라 항바이러스, 항증식, 항암 등 다양한 분야에서 효능이 연구되고 있어, 해당 물질에 대한 관심이 집중되고 있다. 살충 물질로서의 DTXs는 여러 곤충에 있어 병원성을 나타내는 것이 확인되었으나, 해충 으로서 전 세계적으로 심각한 경제적 피해를 일으킴과 동시에, 화학 살충제 저항성 문제가 야기되고 있는 목화진 딧물에 대해서는 아직 DTXs의 역할이 연구되지 않고 있는 실정이다. 본 연구에서는 목화진딧물에 대해 곤충병원 성 진균 Metarhizium spp.의 병원성 발현에 DTXs가 미치는 역할을 간접적으로 확인하기 위해, qPCR을 활용하여 진균 처리 후 목화진딧물 사망 시간과 관련하여 충체 내 DTX 합성효소의 발현을 비교 분석하였다.

Mycoviruses are a group of viruses that infect filamentous fungi. While most hosts infected with mycoviruses do not show any symptoms. In some cases, mycoviruses induce various phenotypic changes include alterations in morphology, drug resistance, pathogenicity, virulence, sporulation, and growth. Entomopathogenic fungi are one of the integrated pest management agents as an alternative to conventional insecticides. Mycoviruses have the potential as supportive agents, enhancing the efficiency of the insecticidal activity of the fungi. Studies about mycoviruses themselves and their interaction with their hosts, especially entomopathogenic fungi, are needed to realize their full potential. In this work, the sequence of the dsRNA element isolated from the entomopathogenic fungus Metarhizium pinghaense 4-2 strain was determined. Through this study, we report the sequence of a dsRNA virus isolated from the Metarhizium pinghaense for the first time. In further studies, the ORF of the mycovirus that induces a phenotype change in the host will be researched.

마이코바이러스는 곰팡이를 감염시키는 바이러스로 자낭균류, 담자균류 및 난균류에서 주로 발견되며 일부 의 경우 곰팡이의 표현형에 영향을 끼치는 것으로 알려져 있다. 이번 연구에서는 대한민국 토양 샘플에서 분리된 65개의 자낭균류 및 접합균류 균주의 전체 핵산을 추출하고, 전기영동을 통해 바이러스 특이적 밴드를 스크리닝 하였다. 스크리닝 결과 65개의 균주 중에서 Tolypocladium spp. 균주 2개와 Marquandomyces marquandii 균주 1개에서 바이러스 특이적 밴드를 발견하였다. 그 후, Cellulose Chromatography를 이용하여 double-stranded RNA 를 분리하고 DNase I 및 S1 Nuclease 처리를 통해 DNA와 single-stranded RNA를 제거하여, Tolypocladium sp. 균주 1개와 Marquandomyces marquandii 균주에서 발견한 특이적 밴드가 dsRNA임을 확인하였다. 추후 virus-free isogenic line을 확보하여 virus 유무에 따른 표현형 변화를 확인하고, 마이코바이러스와 곰팡이 간의 상호작용에 관해 연구할 계획이다.

Aphids are well-known insect pests that, due to their feeding habits, affect various crops' productivity and marketability. Additionally, they cause significant damage worldwide as vectors of viruses. Chemical pesticides are widely used to control these agricultural pests. However, due to resistance to various chemical pesticides, pest control is becoming difficult in agricultural environments. Entomopathogenic fungi such as the genus Metarhizium and Beauveria are being extensively researched as alternatives to these chemical pesticides. In this study, the mycological properties of isolated from soil Metarhizium pemphigi IPBL-H and the insecticidal activity of this strain against Myzus persicae and Aphis gossypii were evaluated for potential of development as a biopesticide.

뿌리응애류는 양파, 마늘, 생강, 백합 등의 뿌리를 가해한다. 최근 백합재배지에서는 질경이모자이크바이러 스(Plantago asiatica mosaic virus, PLAMV)에 의한 잎의 괴사 피해가 확산되고 있다. 태안 백합재배지에서 PlAMV 가 감염된 백합을 채집하여 구근을 조사한 결과, 식물체 당 뿌리응애 100개체 이상이 발견되었으며 Rhizoglyphus robini로 동정되었다. 이 종이 PlAMV의 보독여부를 확인하기 위해 채집된 R. robini에서 RNA를 추출하여 RT-PCR로 진단한 결과, 모든 개체에서 PlAMV가 확인되었다. 본 연구는 뿌리응애가 백합 구근 뿌리를 먹으면서 만든 상처를 통해 PlAMV가 전염될 수 있다는 가능성을 제시한다.

Cryptotympana atrata belongs to the family Cicadidae, has long been recognized as a damaging plant-sucking pest, and is distributed in East Asian countries. In addition, their cries cause direct harm to us through noise pollution and also reported twig damage in the forest environments. In this study, we isolated strains of the entomopathogenic fungus Metarhizium that occurred from C. atrata collected this year. Here, we provide the morphological character and molecular phylogenetic relationship of this species. This is the first record of the entomopathogenic fungus Metarhizium viridulum isolated from C. atrata in Korea and provides a candidate strain with potential use for biological agents.

The Korea Laboratory Accreditation Scheme (KOLAS) is the national accreditation body responsible for providing accreditation services to testing and calibration laboratories. The primary objective of KOLAS is to promote the quality and reliability of laboratory testing by providing nationally and internationally recognized accreditation services. Laboratories accredited by KOLAS are required to meet rigorous international standards set by the International Organization for Standardization (ISO) and are subject to regular assessments to ensure ongoing compliance with the standards. KOLAS accreditation is highly regarded both domestically and internationally, and is recognized for providing high-quality and reliable testing services. The nuclear analysis laboratory at KINAC has been working to establish a quality management system to ensure the external reliability of analytical results and to secure its position as an authorized testing agency. To achieve this, a detailed manual and procedure for nuclear material analysis were developed to conform to the international standards of ISO/IEC 17025. This study presents the preparation process for establishing the management system, focusing on meeting technical and quality requirements for the implementation of the ISO/IEC 17025 standard in the KINAC nuclear analysis laboratory, specifically in the field of chemical testing (dosimetry, radioactive, and neutron measurement subcategories). The preparation process involved two tracks. The first track focused on satisfying technical requirements, with Thermal Ionization Mass Spectrometer (TIMS) and Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) selected as the major equipment for analysis. Analytical methods for determining isotope ratios and concentrations of nuclear materials were determined, and technical qualification was ensured through participation in proficiency test programs, inter-experimenter comparison tests, and uncertainty reports. The second track focused on developing the quality system, including quality manuals, procedures, and guidelines based on the requirements of the ISO/IEC 17025 standard. Various implementation documents were produced during the six-month pilot period, in accordance with the three levels of documents required by the standard. Implementation of ISO/IEC 17025 is expected to have a systematic quality management process for the analysis lab’s operations and to increase confidence in KINAC’s nuclear analysis.

Ball stud parts are manufactured by a cold forging process, and fastening with other parts is secured through a head part cutting process. In order to improve process quality, stabilization of the forging quality of the head is given priority. To this end, in this study, a predictive model was developed for the purpose of improving forging quality. The prediction accuracy of the model based on 450 data sets acquired from the manufacturing site was low. As a result of gradually multiplying the data set based on FE simulation, it was expected that it would be possible to develop a predictive model with an accuracy of about 95%. It is essential to build automated labeling of forging load and dimensional data at manufacturing sites, and to apply a refinement algorithm for filtering data sets. Finally, in order to optimize the ball stud manufacturing process, it is necessary to develop a quality prediction model linked to the forging and cutting processes.

Phosphate coating is applied to the surface of the round bar material used in the multi-stage cold forging process for the purpose of lubrication. The film characteristics are determined according to the conditions of the phosphate film treatment process. In this study, the film properties according to the phosphate treatment conditions were defined as the coefficient of repeated friction and quantitative analysis was performed. Different friction behaviors were exhibited depending on the film properties, suggesting that optimization of the phosphate film treatment conditions is possible based on this. Finally, as a practical example, friction behavior according to the film characteristics was applied to the automotive engine bolt forming process. As a final conclusion, the need for linkage analysis with phosphating conditions for optimizing the forging process was raised. In addition, it can be seen that damage to the phosphate film should be considered in the process of predicting the limiting life of the die.

Most of automobile steering parts are manufactured through the multi-stage cold forging process using round-bar drawn materials. The same process is applied to the ball stud parts of the outer ball joint, and various research activities are being carried out to reduce the extreme manufacturing cost in order to survive in the limitless competition. In this paper, we present a quantitative prediction method for the limiting life of the die as a method for cost reduction in the multi-stage cold forging process. The load on the die was minimized by distributing the forming load based on process optimization through finite element analysis. In addition, based on the quantitative prediction algorithm of the limiting life of the die, the application of the split die and the optimization of the phosphate treatment of the material surface are presented as a conclusion as a method to improve the limiting life of the die.

A wire rod, a material for multistage cold forging, is subjected to spheroidization and low annealing heat treatment to secure formability, and a phosphate coating treatment on the material surface to secure lubricity. The film layer produced by the phosphate treatment process is involved in adhesion to the material surface, adhesion to the forging die surface, and lubricity. This results in the increase or decrease of the forming load and the increase or decrease of the die life in the cold forging process. In particular, as the cold forging process progresses, the phosphate film is damaged and the original performance is deteriorated, so there is a high possibility of process defects. In case of excessive damage, the film is completely lost and die soldering occurs. Therefore, in this study, quantitative criteria for phosphate film damage are presented and the effect on the cold forging process is analyzed based on this to improve process analysis prediction accuracy. Therefore, in this study, quantitative criteria for phosphate film damage are presented, and based on this, the friction coefficient in the multi-stage cold forging process is to be derived.