This study was aimed to isolate bacterial inoculants producing chitinase and evaluate their application effects on corn silage. Four corn silages were collected from four beef cattle farms to serve as the sources of bacterial inoculants. All isolates were tested against Fusarium graminearum head blight fungus MHGNU F132 to confirm their antifungal effects. The enzyme activities (carboxylesterase and chitinase) were also measured to isolate the bacterial inoculant. Based on the activities of anti-head blight fungus, carboxylesterase, and chitinase, L. buchneri L11-1 and L. paracasei L9-3 were subjected to silage production. Corn forage (cv. Gwangpyeongok) was ensiled into a 10 L mini silo (5 kg) in quadruplication for 90 days. A 2 × 2 factorial design consists of F. graminearum contamination at 1.0104 cfu/g (UCT (no contamination) vs. CT (contamination)) and inoculant application at 2.1 × 105 cfu/g (CON (no inoculant) vs. INO (inoculant)) used in this study. After 90 days of ensiling, the contents of CP, NDF, and ADF increased (p<0.05) by F. graminearum contamination, while IVDMD, acetate, and aerobic stability decreased (p<0.05). Meanwhile, aerobic stability decreased (p<0.05) by inoculant application. There were interaction effects (p<0.05) on IVNDFD, NH3-N, LAB, and yeast, which were highest in UCT-INO, UCT-CON, CT-INO, and CT-CON & INO, respectively. In conclusion, this study found that mold contamination could negatively impact silage quality, but isolated inoculants had limited effects on IVNDFD and yeast.

본 연구에서는 대표적인 신선 잎채소류인 상추의 세척 단계에서 초음파 (37 kHz)와 염소 (100~300 ppm)의 병용 처리 후 냉장 ~ 실온저장 (10~25C)에 따른 이 식품 중의 Salmonella Typhimurium 의 성장예측모델을 개발하였다. 1차 모델 개발을 위해 Gompertz 방정식을 활용하여 각기 다른 실험 조건에서의 S. Typhimurium의 생육도 (SGR과 LT)를 조사했다 . 본 방정식에 의한 1차 모델 개발시 R2가 0.92 이상으로 우수하게 나타났으며 저장온도가 낮을수록 초음파에 사용된 염소의 농도가 높을수록 SGR 값은 감소하였고 LT 값은 증가하였다 . 이를 바탕으로 2 차 polynomial 모델을 개발하여 다양한 통계적 지표 (R2, MSE, Af 및 Bf)를 통해 분석한 결과 개발된 모델의 적합성을 확인할 수 있었다 . 따라서 개발된 모델이 초음파와 염소의 병용 세척에 따른 저장 중 상추에 대한 S. Typhimurium의 성장예측모델로 사용 가능하다고 판단되어지며 , 신선 잎채소류에서의 식중독을 예방하고 미생물학적 위생관리기준을 설정하는데 기초자료로 활용될 수 있을 것으로 사료된다 .

Ganoderma lucidum has been reported to have various biological activities including antioxidant activity. The objective of this study was to compare the antioxidant effects between Ganoderma lucidum (GL) and antler-shaped fruiting body of Ganoderma lucidum (AGL). In vitro antioxidant activities were examined by 2,2-diphenyl-picrylhydrazyl hydrate (DPPH) and 1,2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activities of GL and AGL ethanol extracts. In the DPPH and ABTS radical scavenging activities of GL and AGL ethanol extracts, antioxidant activities of AGL extracts (IC50, 66.94 μg/ml and 131.23 μg/ml) was showed higher than GL extracts (IC50, 83.93 μg/ml and 164.54 μg/ml). Total polyphenol content and total flavonoid content (38.00 g GAE/kg extract and 11.58 g NE/kg extract) of AGL were found higher as compared to GL (34.23 g GAE/kg extract and 3.46 g NE/kg extract). In summary, the results of this study demonstrate that AGL extracts had higher antioxidant activities to GL.

The purpose of this study was to investigate whether or not the antler-shaped fruiting body of Ganoderma lucidum (GL) has an anti-inflammatory effect on lipopolysaccharide (LPS)/interferon-γ (IFN-γ)-activated RAW 264.7 macrophage-like cells. To evaluate the anti-inflammatory effects of GL, we examined the inflammatory mediators such as the production of nitric oxide (NO) and the expression of mitogen-activated protein kinase (MAPK), activator protein 1 (AP-1), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), and interleukin-6 (IL-6). LPS/IFN-γ-induced cellular NO production was significantly decreased in GL-treated RAW 264.7 cells. Moreover, Western blotting analysis results demonstrated that reduced protein expression of MAPK families (such as extracellular signal-regulated kinase, c-Jun amino-terminal kinase, and p38 MAPK) and AP-1-targeting inflammatory enzymes (iNOS, COX-2, IL-1β, and IL-6). These results indicated that GL modulates the MAPK/AP-1 signal pathway in inflammatory process. In conclusion, the present study provides important evidence that GL can potentially be used to reduce LPS/IFN-γ-induced inflammatory response by inhibiting the MAPK/AP-1 signaling pathways.

Chrysanthemums (Dendranthema grandiflorum ‘Iwanohakusen’) were grown in a greenhouse with complete nutrient solution system to investigate the effect of silicon (Si) supplement on salt induced deleterious effects in chrysanthemum plants. The experiment was conducted in plastic pots supplemented with a mixture of upland soil : leaf mold : river sand (3:3:4, v:v:v). Si and salinity were treated in combination with two levels of NaCl (0 and 100 mM) and two sources of silicon (K2SiO3, KSi and silicate fertilizer, SiF) at the same concentration (1.8 mM Si) by weekly-drenching for 12 weeks. Chrysanthemum plants supplemented with Si increased in fresh and dry matter enhancing water content and salinity tolerance. The plants grown under salt stress produced less fresh and dry matter than control plant. However, Si supplement to plants under salt stress ameliorated negative effects of salt stress. In soil, EC and NaCl increased by salt stress were mitigated by Si supplement. Salt stress significantly decreased the contents of K and P in leaf, but Si supplement under salt stress significantly recovered the decreased contents with enormous desorption of K and P in soil. Added Si significantly increased content of available SiO2 with its adsorption by salt stress in soil, which was directly related to Si accumulation in leaf. However, Si uptake by roots was suppressed by salt stress irrespective of Si supplement. Si supplement did not ameliorated the negative effects of salt stress on chlorophyll content and membrane integrity in leaf of chrysanthemum plant although significantly increased Si content in leaf, but reversed pest (Liriomyza trifolii) resistance to above-control level.

Pine wood nematode, Bursaphelenchus xylophilus, is a causative organism to induce pine wilt disease in many varieties of pine trees. Until 2006, Monochamus alternatus had been known as the only insect vector of pine wood nematode in Korea which targeted on Pinus densiflora (Japanese red pine) and P. thunbergii (Japanese black pine). However, pine wilt disease was also reported from Korean pine tree (Pinus koraiensis) in 2006 and we found another insect vector, M. saltuarius, was involved to transmit pine wood nematode. Both Monochamus species were confirmed to transfer pine wood nematode to their hosts but, there is no detail information about other transmitted nematode. Especially Bursaphelenchus mucronatus is common species transmitted by Monochamus species which is morphologically closed to B. xylophilus. Moreover B. mucronatus have two genotypes; one is East Asian type and the other is European type. Both genotypes of B. mucronatus were found in Korea but, the host and vector information related to the genotypes of B. mucronatus was not clear. Monochamus saltuarius was collected from three different geographical locations and nematodes were extracted and identified. For the identification of the juveniles, nematode DNA was extracted and ITS-RFLP analysis was done by PCR and gel electrophoresis. The selected enzymes were Hinf I, Alu I, Msp I, Hae III, Rsa I. Most of Bursaphelenchus species carried by M. saltuarius, which collected from pine wilt disease-free area, was determined as European type of B. mucronatus. We will compare the nematode species and genotypes carried by M. alternatus and M. saltuarius. In addition the rate of nematode carrying insect and the average number of nematode per single insect will be counted and compared.

The fragile X mental retardation (FMR) syndrome is the largest source of inherited mental retardation. The syndrome is usually caused by the transcriptional silencing of fragile X mental retardation gene (FMR-1). An 18 years old male wascompla띠ing of multiple toαh missing and abnormal facial profùe, of which signs were matemally dominant in his family. In the C)π。gene디c analysis the pa디ent and his parents did not show any discontinuity in the long arm end of X chromosome, but in the PCR produαs targeω19 the CGG repeat sequence in the 5' untranslated region of FMR gene both the patient'’ s and his mother' s gDNAs produced a normal and an extra bands, sized about 400 and 800 bps, respectively, while the his father' s gDNA produced only one normal band, sized about 400 bps. 까1US ， we suppose that the pa디.ent has heterozygotic alleles of FMR gene inherited matemally, and that the patient s FMR gene was in a premutated state relevant to the dentofacial abnormalities.

This study was conducted to compare the volatile flavor compounds of Artemisia annua L. after extraction by simultaneous steam distillation extraction (SDE) and solid-phase micro extraction (SPME) followed by gas chromatography-mass spectrometry (GC-MS) analysis. Via SDE and SPME processes, 79 (1,254.00 mg/kg) and 39 (488.74 mg/kg) compounds were identified respectively. The compounds extracted by SDE included 27 alcohols, 13 aldehydes, 22 hydrocarbons, 3 esters, 12 ketones, 1 oxide and 1 N-containing compound, on the other hand, using the SPME method, 7 alcohols, 5 aldehydes, 1 ester, 18 hydrocarbons, 7 ketones, and 1 oxide were extracted. The major volatile flavor compounds of Artemisia annua L. isolated by the two methods were caryophyllene oxide, -caryophyllene, camphor, -selinene, -muurolene, 1,8-cineol, (E)-pinocarveol and pinocarvone. β β γ The sesquiterpene named caryophyllene oxide was the most abundant volatile flavor compound with relative contents of 234.16 mg/kg and 195.44 mg/kg obtained by the SDE and SPME methods, respectively. Among the identified volatiles, sabinene, β-pinene, α-terpinene, γ-terpinene, yomogi alcohol, myrtenol, (Z)-nerolidol, p-cymen-8-ol and eugenol were detected by the SDE method only while (E)-anethole and α-cubebene were detected by the SPME method only. This study confirmed that the composition and contents of the volatile flavor compounds vary between different extraction methods. More volatile flavor compounds were identified using the SDE method than the SPME method.

Background : Ganoderma lucidum is a non-toxic, medicinal mushroom, which is known to possess anti-inflammatory and immunomodulating activities. However, the effects and mechanism of action of Ganoderma lucidum on lipopolysaccharide (LPS) and interferon-gamma (IFN-γ)-induced nitric oxide (NO) production and its-related cytokine expression are not yet fully understood. This study aimed to evaluate the effect of Ganoderma lucidum on NO production and NO-mediated pro-inflammatory cytokine expression in LPS/IFN-γ-induced RAW 264.7 macrophage-like cells. Methods and Results : The results showed that Ganoderma lucidum inhibited inducible NO synthase (iNOS) expression of RAW 264.7 macrophage-like cells at non-cytotoxic concentrations probably through the reduction of reactive oxygen species (ROS) production. After pre-treatment of cells with non-toxic doses of Ganoderma lucidum; NO production was significantly decreased. Moreover, Ganoderma lucidum treatment suppressed LPS/IFN-γ -stimulated pro-inflammatory cytokine secretion, including interleukin-1β and interleukin-6, in a dose-dependent manner. Conclusion : Taken together, these results indicate that the anti-inflammatory activation of Ganoderma lucidum in LPS/IFN-γ-stimulated macrophages might be due to abrogation of NO-dependent cytokine release by impairment of iNOS expression via ROS generation.

"Juan1" is a new japonica rice variety of mid-late maturing, tolerance to lodging and adaptable to direct seeding which was developed by the rice breeding team of National Institute of Crop Science, RDA, in 2005. This variety is derived from a cross between Ilpumbyeo, a variety of good-eating quality and high yield, and SR18392-HB683-104. The Juanl has heading date of August 19 at ordinary planting in the middle plain. It has semi-erect plant type with culm length of about 80cm and tolerance to lodging. This variety shows susceptible to blast, bacterial blight, virus diseases and brown planthopper. The milled rice kernels of Juanl are translucent with non-glutinous endosperm, clear in chalkiness and medium short grain. It has also low amylose content of 18.8%, low gelatinization temperature and good palatability. The average milled rice yield of this variety was 5.68 MT/ha at ordinary planting in local adaptability test for three years from 2003 to 2005. Juan! would be highly adaptable to middle plain and mid-eastern coastal region of Korea.

A new spray chrysanthemum cultivar, 'Plaisir D'Amour' was released by National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), in 2006. The cross was made in 2003 between '01B1-355', a breeding line of NHRI and `01B1-440', a breeding line of NHRI. After evaluation for the characteristics including shading culture in summer and retarding culture in spring between 2004 and 2006, final selection was made. The natural flowering time of 'Plaisir D'Amour' is late October, but year-round flowering is possible by shading and illumination. It has single type flowers with mixed color by dark pink and white edged. It shows excellent flower setting and early blooming with multi-floret. The diameter of flower is 5.4 cm. The number of flowers per stem is 15.5 and number of petals per flower is 21.5. The days to flower under short day treatment is about 56 and its vase life is 21 days in autumn season.

A new spray chrysanthemum cultivar, 'Pink PangPang' was released by National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), in 2006. The cross between 'Restone' and 'Lollipop' was made in 2003. After evaluation for the characteristics including shading culture in summer and retarding culture in spring between 2004 and 2006, final selection was made. The natural flowering time of "Pink PangPang" is late October, but year-round flowering is possible by shading and illumination. It has pompon type flowers with light pink petals and resistance to white rust. The diameter of flower is 4.3 cm. The number of flowers per stem is 13 and the days to flower under the short day treatment is about 44 in summer season.

A new cultivar ofDianthus caryophyUus "Dreambyul" was selected from the progenies ofa cross "Zenga" and "Euge-nia" in 1999 at the National Horticultural Research Institute, Rural Development Administration. It was fmally selected in 2002after the investig

A new cultivar of Dianthus caryophyHus "Orion" was selected from the progenies of a cross "Rossini" and "Tropicshort node of upper shoot. Optimum temperature for its growth are over 8"C at night and under 25"C at day in summer. The cul- tivar was applied