본 연구는 미성숙, 성숙 단계의 돼지 난포란이 유리화 동결에 의한 동결보존이 가능한지를 조사 하고자 실시하였다. 난포란은 세포질 내 지방구를 분극시키기 위해 원심분리를 실시하였고, 미세조작기를 이용하여 지방구를 제거하였다. 돼지 난포란을 CB 처리하여 원심분리 후 지방구를 제거한 지방제거구(Delipated), CB 처리 후 원심분리만 하여 지방구를 분극시킨 원심분리구(Centrifuged), 아무처리도 하지 않은 대조구(Control)를 EM grid

대두 배아로부터 saponin을 분리·정제하여 유리기 억제효과 및 동물의 암세포에 대한 세포 독성을 조사하였다. 유리기 억제활성에 대한 결과를 보면 대두 배아 saponin을 0.5 및 1.0% 씩 첨가한 그여 식에서 4주 동안 사육한 후 간장을 절취하여 간장 획분의 활성산소 및 항산화 관련 효소에 대하여 평가하였다. 간장의 mitochondria 및 microsome 획분의 hydroxy radical (·OH)의 생성에 미치는 영향에서 급여식에 사포닌을 첨가한 급여군에서 ·OH기의 생성억제 효과를 나타내었다. 간장의 microsome 획분에서도 0.5 및 1.0% 투여군에서 H_2O_2 생성억제 효과가 인정되었다. 또한 간장의 cytosol획분에서 O_2· 의 생성은 대조군에 비하여 현저한 억제 효과를 나타내었다. 대두 배아 사포닌의 세포독성을 P338 (mouse lyoupoid neoplasm) 과 L1210 (mouse leukemia) 세포주를 사용하여 실시한 결과 200 ㎍/mL 농도에서 높은 성장억제효과를 나타내었다. 그리고 처리 농도에 따른 대두 배아 추출물이 P338과 L1210에 대한 성장 효과 실험에서도 200㎍/mL에서 높은 성장 억제 효과가 나타났으며 농도가 높을수록 완전히 억제되는 것을 볼 수 있었다.

In order to establish the processing condition of rapid- and low salt-fermented liquefaction of anchovy (Engrulis japonica), effect of temperature on crude enzyme activity of anchovy viscera, pretreatment conditions, and the minimum content of adding NaCl were investigated. The minimum limitation of NaCl content for anchovy liquefaction was 10%. Sample A(water adding, heating, adding 10% NaCl): chopped whole anchovy adding 20% water and then heating for 9 hrs at 50℃ and then adding 10% NaCl and then fermented at room temperature(8-29℃) for 180 days. Sample B(water adding, heating, adding 13% NaCl): chopped whole anchovy adding 20% water and then heating for 9 hrs at 50℃ and then adding 13% NaCl and then fermented at room temperature for 180 days. Sample C(adding 13% NaCl): chopped whole anchovy and then adding 13% NaCl and then fermented at room temperature for 180 days. Sample D(adding 17% NaCl): whole anchovy adding 17% NaCl and then fermented at room temperature for 180 days. The content of free amino acids such as aspartic acid, serine and threonine fluctuated severely according to the pretreatment methods. Possibly they might be recommend quality indices of standardization for salt-fermented liquefaction of anchovy. As for the relation between fermentation period(X) and individual free amino acid(Y), five kinds of free amino acids such as glutamic acid, valine, glycine, lysine, and alanine showed highly significant in their coefficient of determination in most of samples. They might be recommend as quality indices for salt-fermented liquefaction of anchovy during fermentation. The difference of taste between products of the rapid- and low salt-fermented liquefaction and the traditional salt-fermented liquefaction were caused by their composition of the free amino acids ratios, in which were umami, sweet, and bitter taste in the extracts of anchovy during fermentation. The appropriate fermentation period of the sample A was shorten 30 days than the sample B and 60 days than the samples C and 90 days than the sample D in the processing of anchovy.

This study was performed to evaluate whether vitrification method using ethyle glycol and eletron microscopic (EM) grid could be used far the cryopreservation of human oocytes in ART program. Surplus oocytes were obtained from consented IVF patients. These surplus human oocytes were frozen with our vitrification method, Oocytes were exposed to 1.5M ethylene glycol (EG) in DPBS far 2,5 minutes, followed by 5.5M EG plus 1.0M Sucrose in DPBS for 20 seconds. Then oocytes were transferred onto the EM grid and the grid was plunged into LN2 for storage. For thawing, oocytes containing EM grid were sequentially transferred in 1.0M, 0.5M, 0.25M, 0.125M and 0 M sucrose in DPBS solution at the intervals of 2.5 minutes. Thawed and survived oocytes were provided for ICSI. Embryos from vitrified oocytes were transferred to uterus of the patient on 4 to 5 days after ovulation in natural cycles of on 15 to 17 day of hormone replacement cycles. A total of 370 oocytes from 26 patients were thawed and 159 (43.0%) of them survived. One hundred thirty four oocytes (84.3%) were fertilized normally and 126 pre-embryos were transferred to 26 patients, resulting in 5 clinical pregnancies. The pregnancy rate per transfer was 19.2% and implantation rate was 4.0%. Among the five pregnant, 4 patients delivered 4 healthy babies and the one patient was 32-week ongoing pregnancy. From this results, vitrification using ethylene glycol as cryoprotectant and EM grid is a rapid and simple method that can be effectively applied for the cryopreservation of human oocytes in ART program.

Selection of oocyte cryopreservation method is a prerequisite factor for developing an effective bank system. Compared with slow freezing method, the vitrification has various advantages such as avoiding intracellular ice crustal formation. In our previous, we attempted to employ a vitrification method using ethylene glycol and an electron microscope grid for cryopreservation of mouse oocytes. However, A high incidence of spindle and chromosome abnormalities was detected in thawed oocytes after vitrification. We examined whether the addition of a cystoskeleton stabilizer Taxol , to the vitrification solution could promote the post-thawed survival and subsequent development of stored oocytes. More oocytes developed to the 4-cell (44.7% vs. 69.7%), 8-cell (31.8% vs. 64.2%), morula (24.7% vs. 54.3%), and blastocyst (20.3% vs. 49.2%) stages after the addition of Taxol to the cryoprotectant than after no addition. 21 and 26 mouse pups were born after transfer of blastocyst derived from oocytes vitrified without and with Taxol. The addition of Taxol to vitrification solution greatly promoted post-thaw preimplantation development of ICR morose oocytes.tes.

To develop an effective vitrification method, we examined the use of a conventional straw as vessel fur vitrification of mouse oocytes, and to compare the post-thaw survival and chromosome configuration of these oocytes with those vitrified in grids. Intact cumulus-enclosed oocytes were vitrified with DPBS with 5.5 M ethylene glycol and 1.0 M sucrose, and loaded into straws and onto eletron microscopic copper grid fur storing in liquid nitrogen. Intact vitrified and thawed oocytes were karyotying for chromosome. The rates of post-thawed survival were 88.5% in vitrified oocytes with straws, and 83% in vitrified ooctyes with grids. Vitrified and thawed oocytes with straws and grids were increased chromosomal abnormality (31.4% and 30.9%) compared with fresh oocytes (17.8%). The conventional straws can be used as vessel for vitrification to prevent of inflection in liquid nitrogen.

우리나라의 전통 발효식품인 고추장의 관능성 및 기능성을 더하여 품질을 향상시키기 위하여 전분질 원료인 찹쌀무게에 대하여 2%, 4%, 6% 및 8%의 다시마 분말을 첨가한 후 대조구와 함께 30℃에서 120일간 숙성시키면서 유리아미노산과 지방산 조성 및 관능적 특성을 검토하였다. 숙성 중 대조고추장과 다시마고추장 모두에서 아미노질소는 서서히 증가하는 것으로 나타났으나 아미노질소의 경우 대조고추장에 비해 다시마고추장에서 다소 낮게 나타났다. 숙성 30일째의 아미노질소량은 각각 171.3l㎎%, 172.l0㎎%, 174.l8㎎%, 185.60㎎% 및 161.70㎎%로 최고값을 보였다. 유리아미노산 중에서는 glutamic acid의 함량이 가장 높은 것으로 나타났는데 숙성이 진행될수록 증가하였다. 또한 arginine. aspartic acid. preline. serine. leucine. lysine도 비교적 높은 함량을 가졌다. 고추장에서 분리 확인된 지방산은 lauric acid- myristic acid, palmitic acid, atearic acid, oleic acid, linoleic acid 등이었고 이들 중 oleic acid의 조성비율이 가장 높았고 palmitic acid가 다음으로 높았으며 stearic acid. lauric acid, myristic acid의 경우 숙성 후기로 갈수록 산화되어 감소하는 경향을 보였다. 숙성 60일과 120일에 실시한 관능검사의 결과 8% 다시마 첨가구를 제외하고는 대조고추장과 비교하여 다시마 첨가가 고추장의 관능적 특성에 영향을 미치지 않았으며 다시마 첨가수준이 높아질수록 높은 값을 나타내어 고추장에 다시마를 6% 점도 첨가하는 것이 적당하였다.

고온 안정성의 유리계로 알려진 회토류 알루미나 규산염계중, Nd2O3-Al2O3-SiO2(NdAS)계 유리의 응용범위를 찾고자 결정화유리를 제조하여 그 물성의 특성을 평가하였다. NdAS에 결정화제로 TiO2를 첨가하여 내부결정화를 유도하여 생성된 결정화유리에 대하여 결정상과 잔류유리의 물리적, 열적, 기계적 물성을 측정하였다. NdAS-TiO2유리계는 열처리와 조성 조건에 따라 생성된 표면 및 내부결정상은 같은 결정상을 갖는 것으로 X선회절의 결과로 확인되었으나, 알려 있지 않은 결정상으로 내부결정의 경우, 원자구성비는 Nd4.6Si7.2Al4.0Ti2.4O32이었다. 결정화유리의 선팽창계수는 5.4~6.2×10-6/˚C 정도로 경정성장이 일어날수록 증가되었다. 결정화유리중의 결정상의 경도와 탄성계수는각 각 12GPa, 220Gpa으로 나타난 것을 고려한다면 내부결정화에 의한 결정화유리의 물성은 고온 구조용 재료로 활용도가 넓을 것으로 본다.

Due to its low density, good mechanical properties and chemical inertness, glassy carbon(GC) has been studied for appications in several fields. A raw thermosetting resin of furanic resin was polymerized with a curing agent of p-toluenesulfonic acid monohydrate. The maximum yield of GC was obtained at the curing agent content of 1.0 wt% in furanic resin. In order to make thick GC, the affect of graphite filler addition to the furanic resin was investigated. The density and electrical resitivity of GC after graphitization were 1.45 g/cm3 and 47 ×10-4 Ω · cm respectively and the amorphous structure of GC was confirmed by XRD profiles with very broad peaks comparable to those of graphite at 206˚ and 45˚.