Rice plants segregating for opaque endosperm were selected and tested in every generation from F₅ to F₉ of a CB243/IR50 cross. Six near isogenic lines(NIL_s) showing difference in opaqueness, of which three lines have opaqu

This study characterized the genetic variations of 13 populations of Asplenium antiquum and its relative species using randomly amplified polymorphic DNA (RAPD) markers. A total 88 scorable RAPD bands were generated by the 12 random oligo primers and were analyzed by Nei and Li's genetic distance. High genetic variability was detected between A. antiquum and A. nidus, with the range from 0.568 to 0.682. And slightly low genetic variations showed within the populations of same species. Seven populations of A. antiquum showed slight differences (0.000-0.216), and five populations of A. nidus showed similar low genetic variations (0.114 to 0.171). Two individuals from Sup-seom Island which are growing in might be the regenerated one from abroad. A. antiquum were clustered as two groups (Group I, Group II) by UPGMA phenogram. And five populations of A. nidus were clustered as two groups correlated with geographical distribution. The RAPD data was very useful to define the genetic variations and to discuss the phylogenetic relationships among A. antiquum and the related species..

Two rice varieties, 'Oochikara' with large grain and 'Hwayeongbyeo' and their progenies (F1 , F2 , B1 and B2 ) were tested to understand gene action of morphological traits of rice grain and their relationships. The evaluated traits were 1,000-grain weight, grain length, width, thickness, length-width ratio and chalkiness of brown rice. Correlation between grain weight and chalkiness was highly significant in the all progenies, and grain length were not associated with width and thickness in an F2 population. Scaling test and jonit scaling test revealed that inheritance of grain traits were fitted to additive-dominance model without epistasis. Additive effects for the traits were much greater than the dominance effects.

군산지역에 있는 호수와 양식장에서 채집된 붕어(Carassius carassius)의 혈액으로부터 추출된 genomic DNA를 무작위 primer를 이용한 PCR-RAPD 방법에 의해서 유전적 차이를 확인하고자 하였다. 12개 primer 중에서 6개를 이용한 결과 호수산 붕어의 경우 primer 당 약 2.1 polymorphic bands가 나타났고, 총 266개의 높은 RAPD marker가 확인되었으며, 0.18에서 0.76의 bandshari

Intraspecific genetic diversity of sixteen accessions of Mogolian Alliums including fifteen species was investigated using randomly amplified polymorphic DNA (RAPD) analysis. Twenty three out of forty primers revealed scorable polymorphism. A total of 440 RAPD markers were generated on the 16 accessions of Mongolian Alliums. Among 440 RAPDs assayed, 439 were polymorphic with a mean polymorphic rate of 99.7%. Unweighted pair-group method using an arithmetic average (UPGMA) cluster analysis using RAPD data separated the 16 Allium accessions into two broad groups at similarity index 0.70. The clustering of the species was closely related with previous classification between A. altaicum and A. fistulosum. In addition, a high genetic similarity was showed between A. cepa and A. tagar.

Genetic Analysis of Photoinhibition in Barley

50여개의 primer를 사용하여 잔대 Adenophora triphylla와 층층잔대 A. radiatifolia Nakai, 그리고 더덕 Codonopsis laceolata Trautv의 지역간, 속간의 차이점과 감별여부를 RAPD법으로 시행한 결과, 잔대와 층층잔대의 차이점은 거의 없었으며, 더덕의 지역차이는 0.889의 유전적거리를 나타내었다. 두 종(種)을 구별할 수 있는 특이 band로는 primer 357, 361, 363, 393 이었으며, 건조약재와 비교하였을 때 재현성이 확인되었고, 또한 잔대와 더덕의 건조약재를 각각 혼합시켰을 때 이를 구별할 수 있는 major band가 뚜렷이 나타나 혼용되어있는 건조약재에서의 감별이 가능함을 알 수 있었다..

Genetic diversity of 47 East-Asian vegetable soybean was characterized by means of agro-morphological traits and RAPD markers. A field trial was conducted to evaluate 14 agro-morphological traits. To study RAPD-based DNA analysis, a total of sixty 10-mer random primers were screened. Of these, 23 polymorphic markers in 16 varieties used for screening. Among 207 markers amplified, 48 were polymorphic for at least one pairwise comparison within the 47 varieties. A higher differentiation level between varieties was observed by using RAPD markers compared to morphological markers. Correspondence analysis using both types of marker showed that RAPD data could fully discriminate between all varieties, whereas morphological markers could not achieve a complete discrimination. Genetic distances between the varieties were estimated from simple matching coefficients, ranged from 0.0 to 0.640 with an average of 0.295~pm 0.131 for morphological traits and 0.042 to 0.625 with an average of 0.336~pm 0.099 for RAPD data, respectively. Cluster analysis based on genetic dissimilarity of these varieties gave rise to 4 distinct groups. The clustering results based on RAPDs did not match with those based on morphological traits. Geographical distribution of most varieties in each of the groups were not well defined. The results suggested that the level of genetic diversity within this group of East-Asian vegetable soybean varieties was sufficient for a breeding program and can be used to establish genetic relationships among them with unknown or unrelated pedigrees.

In order to develop near isogenic lines (NILs) the heterozygous rice plants for alkali digestibility value(ADV) were selected and tested in every generation from F5 to F9 of a cross, Seratus malam/Suwon 345. Finally several sets of NILs, which were six low ADV lines and four medium-high ADV lines, were selected among F10 lines. No differences of the plant growth characteristics, amylose content and protein content of rice grain were found between low and medium-high ADV lines. Rice flour of low ADV lines showed longer gel length in gel consistency test than medium-high ADV lines, and also showed different gelatinization characteristics measured by rapid visco analyzer compared with medium-high ADV lines. The result of genetic analysis indicated that ADV-NILs developed were differed in a pair of major gene controlling ADV, and low ADV was dominant over medium-high ADV.

This study was carried out to obtain the basic information for breeding cold-tolerant rice varieties with high yield-productivity through wide crosses between indica and japonica rice. Genetic analysis was conducted using 55 F1 s obtained from half-diallel crosses among eleven cultivars of various origin including indica and japonica rice. Screening for cold tolerance was done with cold-water irrigation after transplanting until ripening stage. Both general combining ability (GCA) and specific combining ability (SCA) effects were highly significant in all characters associated with dry matter accumulation at 30 and 50days after cold-water irrigation (DAC). The variance of GCA was much larger than that of SCA in plant height, shoot dry weight per plant (DWP), crop growth rate (CGR) and cold-water response index (CRI) of these characters except CRI of shoot dry weight per plant. The DWP, CGR and CRI of these characters of Gaochan 102, Tong88-7 and TR22183 were markedly higher than those of the others. GCA effects of these varieties on DWP, CGR and their CRI were also higher than those of the others, indicating that they are useful as promising parents for breeding cold-tolerant varieties. Analysis of genetic parameters for 11~times 11 half-diallel F1 s revealed that inter-locus gene interaction were concerned in the expression of plant height at 50 DAC, CRI of DWP at 50 DAC, and CRI of CGR, and that intra-locus gene interaction for plant height and the other characters were partial dominance and over-dominance, respectively. Narrow-sense heritability (h2 N ) was the highest in plant height as 0.729, and the lowest in CRI of DWP at 30 DAC as 0.048, suggesting that selection for cold tolerance will be quite effective in case that the selection criterion is the performance itself.f.

재배되고 있는 22 페칸종간의 RAPD분석을 한 결과, Agarose gel상에서 primer당 평균 6개의 DNA단편이 증폭되었다. 증폭된 DNA 단편의 크기 는 100bp에서 1500bp의 범위에 위치하였다. 페칸종간의 유연 관계를 분석하기에 적합한 primer를 선발한 결과 OPA-02, OPA-10, OPA-12, OPB-01이 최적이었다. 4종의 primer를 이용하여 증폭한 PCR산물을 1.5% Agarose gel상에 분획하여 단편의 종류를 분석한 결과 22의 페칸종은 5개의 그룹으로 구분 할 수 있었다. 40종 primer로부터 증폭된 466 DNA단편을 기초로하여 유전적 근연계수를 계산하였던 결과 유사도 값이 가장큰 것은 C. flacra와 Black walnut로 0.90를 나타났으며, Kiowa와 Red hickory, C. tomentosa, C. flacra, Black walnut간에는 유사도 값이 0.31로 가장 작았다. 선발된 4종의 primer를 이용하여 증폭시킨 PCR산물을 Polyacrylamide gel상에 분획한 후 검출된 DNA단편을 분석하였다. 유사도 값이 가장 큰 것은 그룹 V의 Red hickory, C. tomentosa, C. flacra, Black walnut간으로 1.0이었고, Farley와 Pawnee간과 Sturya와 Clarke간은 공히 0.98를 나타내었다. Polyacrylamide gel분획 후 은염색하여 단편을 검출하는 것은 Agarose gel에 비하여 시간, 기술, 경비가 요구되지만 보다 정확한 유전적 배경을 설명할 수 있다고 생각되었다. 또한 Agarose gel 분석, Polyacrylamide gel 분석 및 주성분 분석법에서 Farley, C. tomentosa, C. flacra, Black walnut, C. corpiformis만이 동일 그룹에서 이탈되지 않았다.

Diallel analysis was conducted with FI's derived from crossing in all combinations without reciprocals among six rice varieties; three tropical japonica and three temperate japonica varieties, with different traits associated with panicle and flag leaf. Epistasis was observed in the number of primary branches (PB) per panicle and of spikelets per panicle, while flag leaf length, flag leaf color, PB length and neck node thickness were explained with the additive-dominance model. The estimated genetic mode of flag leaf length and PB length was a positive complete dominance model with high heritability, and that of flag leaf color and neck node thickness was an incomplete dominance model. In particular, tropical japonica varieties with low-tillering and heavy-panicle appear to have higher number of dominant genes for flag leaf length and PB length than temperate japonica varieties.

빈도별 홍수량의 지역분석을 위하여 유역의 지형특성을 독립변수로 이용하는 회귀모형을 검정하였다. 그런데 이들 독립변수들간의 상관관계가 존재할 경우 능형회귀모형이 이용되기도 하는 이 방법은 다중공선성 문제를 극복하는데 적합한 방법으로 알려져 있다. 능형회귀모형을 최적화하기 위해서는 조정변수가 포함되는 비용함수를 최소화하여야 한다. 본 연구에서는 이 최적화를 위하여 유전알고리즘을 이용하였다. 유전알고리즘은 자연 생물의 유전 및 진화과정을 모방한 추계학적 탐색

수도 저하발아성에 대한 유전 양상을 구명하여 저온발아성 품종 육성의 기초 자료를 얻고자 숙기가 다른 세 그룹에서 10품종을 선정 '94년에 15일 간격 4시기로 파종 및 이앙을 실시하여 수량한 종자를 '95년 4~5월에 13℃ 항온 실내 시험과, 건답 직파의 포장 시험으로 저온발아성을 조사하고, 저온발아성 고 1품종, 저 1품종, 중 4품종을 선정 '94 하계에 절영법에 의한 정역이면교잡을 실시하여 얻어진 F1 교배입을 '95년 5월에 13℃ 항온에서 저온발아 시험을 실시하여 얻은 결과를 요약하면 다음과 같다. 1. 재배 시기를 달리하여 수량된 종자의 실내 및 포장에서의 저온발아성 분산분석 결과 품종에 따라 재배시기간에 고도의 유의차가 인정되었다. 2. 실내 13℃ 항온 저온발아계수와 포장의 자연상태 건답직파에서의 출아계수와의 상관분석 결과 고도의 유의한 상관(r=0.5111)이 인정되었다. 3. 조합능력 분석 결과 일반조합능력 및 특정조합능력 모두 고도의 유의성이 인정되었으며, 일반조합능력이 특정조합능력보다 커서 상가적작용이 상위성 작용보다 더 크게 나타났다. 4. 정역차이 분산분석 결과 정역간에 고도의 유의차가 인정되었다. 5. 부친열 공분산/모친열 공분산 Graph에서 6개의 교배열 모두가 모본 효과를 보였으며, Alpha와 Beta 유전 분석 결과, 저온발아성의 정역간 차이는 Alpha 유전의 효과로 나타났다. 6. 평균우성도 분석 결과, 30개 조합 중 17개 조합에서 초월분리를 보였으며, 그 중 9개 조합이 저온발아성이 낮은 쪽으로 초월분리를 보였다.켜야 했다.0~130일이었고 재배가능 기간인 등숙만한기(CLRD)까지는 인제 154일, 수원과 대전 170~180일, 전주, 밀양 및 부산 등지는 180~200일이었다.환경적응성이 낮은 경향이었다.은 모두 경운 유 ㆍ무에 관계없이 시비수준이 높을수록 높은 경향을 보였고, 10일에 최고수준을 보인 후 완만한 감소를 나타냈으며, K+, Ca2+에 비해 Mg2+이 보다 낮은 함량을 보였다.989년산 일반계를 10분도와 12분도로 도정하였을 때 도정도에 따른 밥맛의 차이는 없었다.X>CoOx는 Co3O4 로 존재하고, 반응 전의 경우에는 이와는 다른 chemical state를 보여주었다. XRD 및 XPS 결과를 바탕으로, 촉매표면에 존재하는 Co3O4 의 외부표면이 Co2TiO4 와 CoTiO3 같은 CoTiOx 로 encapsulation되어 있는 모델구조를 제안할 수 있고, 이는 반응시간의 함수로 나타나는 촉매활성에 있어서 전이영역의 존재를 잘 설명할 수 있을 뿐만 아니라, XRD와 XPS에서 얻어진 촉매의 물리화학적인 특성을 잘 반영할 수 있다. 나타냈고, 골격근과 눈 조직에서 피루브산에 대한 LDH의 친화력이 상당히 크므로 LDH가 혐기적 조건에서 효율적으로 기능을 하는 것으로 사료된다.5) and "Cleanliness of clothes ＆ features" (p ＜0.05) of VIP ward were significantly higher than those of a genera