Tlis experiment was to study the effects of application rates of mineral N and cattle slurry on the dry matter yield and botanical compositions of Orchardgrass sward grown in 4 cuttings in a year. Annual rates of mineral N of O(), 120kg((), 240kg(() and 3

본 시험은 경사 척박지에서 초지조성시 양축 농가에서 부수적으로 생산되는 액상구비를 시용하여 목초의 정착과 조기생육을 원활하게 하고 동시에 부숙된 액상구비를 초지에 환원하여 초지상태의 비옥도를 증진시키고자 수원과 여주지방에서 3년간 수행하였다. 1. 목초의 정착율은 오차드그라스 단파구와 혼파구 공히 무처리구보다 처리구가 좋았다(무처리구에 비해 진압, 액비, 시용구, 지오라이트구에서 5% 유의성) 2. 조기생육에서 초장은 무처리보다 처리구가 0.5cm~2.

소 난포란의 체외성숙율과 체외수정후 분할율을 향상시키기 위하여 성숙배양액에 FCS 첨가 효과를 조사하였으며 FCS의 첨가수준은 5-20%였고 사용된 기본배양액은 Dn(-BSA), Ham's F10 및 TCM199이었다. 난포직경 2-6mm 난포로부터 채란된 수정란을 39 배양기에서 28시간 성숙시킨후 동결융해정자 또는 비동결정자로 체외수정시켰다. FCS 무첨가구보다는 첨가구 성숙유리 향상되었으나 FCS 첨가의DM(-BSA)와 Ham's F10간에, 그리

This study was carried out to compare farrowing rate and litter traits for European and American lines with boar sperm frozen in straws. Farrowing rate, litter size and mean pig weght at birth and 21 days were investigated. A total of 36 gilts Landrace, Large white and Duroc were investigated at the Chungnam Provincial Animal Breeding Station. We obtained higher farrowing rate and litter traits for European line boars compared to American line boars.

This study was carried out to produce monozygotic twin calves by transfer of bisected embryos. Four Korean native cattle donors were superovulated with FSH and flushed to collect embryos on day 6 or 7 of the estrus cycle. Morula and early blastocyst embryos showed 1 or 2 grade were bisected with microblade and each set of demi-embryos without zona pellucida were transferred nonsurgically to 10 recipients respectively. The results obtained were as follows; 1. Twenty four demi-embryos (92.3%) were separated from 13 original embryos and among them 20 demi-embryos (83.3%) had normal appearance without severe damage. 2. Four sets of fresh demi-embryos were transferred to 4 recipients and one recipient was twin pregnant 3. Six sets of frozen-thawed demi-embryos were transferred to 6 recipients. Two recipients were pregnant, one of them twin.

본 시험은 농산물의 수입자유화 추세로 들어가고 있는 유휴 경작지에 조사료 자원확보와 날로 심각하게 증가되는 가축분뇨를 유기질 비료로서 재활용하여 환경보전을 목적으로 유휴 논 토양에 조성된 리드카나리그라스의 초지에 액상구비를 시용하여 연 평균 건물수량과 계절적 분포 및 적정 액상구비의 시용수준을 추정코자 실시되었다. 2. 3개의 예취빈도(연간3, 4및 5회 예취)조건에서 연 평균 건물수량은 ha당 8.9~10.9톤을 기록하였는데 3회 예취 이용시에 가장

본 시험은 톨 페스큐가 우점된 방목초지에서 소비구(T; 120-100-100 kg/ha), 보비구(T: 280-200-240kg) 및 보비+보파구(T)등 3개 처리에 3원 교잡종 육성우를 이용하여 1991년 4월 16일부터 11월 1일까지 199일 동안 계절별 목초 생산량에 따라 방목두수를 증.감 조절하여 보충사료 급여없이 계속방목 방법으로 수행되었던 바 얻어진 결과는 다음과 같다. 1. 처리별 식생은 소비구(T)에서 tall fescue가 60~70%

The objective of this study was to produce calves derived from in vitro fertilization of in vitro matured follicular oocytes. Oocytes aspirated from small antral folicles of ovaries obtained at a local slaughter house were matured and fertilized in vitro. At l8hrs after insemination with Korean native cattle semen, oocytes were co-cultured for 6~7 days by utilizing co-culture system with bovine oviduct epithelial cell. After co-culture, good or excellent quality late morulae or early blastocysts were selected by morphological criteria under stereo microscope. Selected embryos were transferred to recipients on day 6 or 7 (estrus = day 0). Recipients were monitored by observation for estrus and rectal palpation after 60 days from embryo transfer. One of them went to term with the birth of a calf. This case is the first production of calf derived from in vitro fertilization in Korea.

This study was carried out to propagate Korean native cattle using beef recipients by embryo transfer. Seven Korean native cattle donors were superovulated with FSH 32mg and Embryos collected from donors were frozen and preserved in National Animal Breeding Institute. Frozen-thawed embryos were transferred to synchronized 40 beef recipients nonsurgically in Daekwanryeong Branch of National Animal Breeding Institute. The results obtained were as follows : 1. Total ova and transferable embryos per donor were 11.4 and 11.1 from 7 donors, respectively. 2. Among 40 recipients transferred with frozen-thawed embryos, 20 were pregnant(50.0%). 3. The pregnancy rate according to time from embryo thawing to transfer was higher when transferred within 3 hours than after 3 hours(57.6% vs. 14.3%). 4. The cow recipients showed slightly higher pregnancy rate than the heifer(53.3% vs. 48.0%). 5. Two grade embryos showed higher pregnancy rate than 1 grade(66.7% vs. 45.2%).

본 시험은 방목이용 초지에서 액상구비(액비) 시용시 가축기호성과 초지 이용효율을 증진시키고자 액비 시용시기를 방목직후(평균 목초 초장 7.5cm), 방목 후 5일째(13.6cm), 방목 후 10일째(18.5cm), 방목 후 15일째(23.5cm)로 달리하고, 액비무시용구를 대조구로 하여 실시되었다. 연간 액비 시용량은 40㎥/ha로 봄철 2회, 여름철 1회, 가을철 1회로 4회 균등 분시하였으며, 전 시험구에서 연간 시비량은 질소 280, 인산 200,

Effects of caffeine, heparin and caffeine-heparin treatments for in vitro capacitation of Korean Native Cattle sperm on acrosorne reaction and viability were studied using the methods of Wells-Awa and Dual stain. The results were summerized as follows: 1. The acrosome reaction of sperm when treated with caffeine after 0 to 4 hrs of preincubation were 11.0~75.7% for Wells-Awa stain, and 14.3~75.55% for Dual stain. True acrosome reaction of sperm for Dual stain was 3.0~29.2%. The viability of sperm was 62. 2~27.2%. 2. The acrosome reaction of sperm when treated with heparin after 0 to 4 hrs of preincubation were 17.0~81.2% for Wells-Awa, and 14.3~75.5% for Dual Stain. True acrosome reaction of sperm for Dual stain was 1.5~26.6%. The viability of sperm was 58.6~35. 8%. 3. The acrosome reaction of sperm when treated with caffeine-heparin after 0 to 4 hrs of preincubation were 13.0~83.2% for Wells Awa, and 11.0~78.5% for Dual stain. True acrosome reaction of for Dual stain was 5.1~26.3%. The viability of sperm was 60.5~30.1%.

With a purpose of finding out the effects of cattle slurry application on thc nutrient contents of herbages and energy productivity in grassland, a field experiment was conducted with two times and five levels of cattle slurry application. The experimenta

With a purpose of finding out the effects of cattle slurry applicatioin on the dry matter yield, yield component, and botanical composition in the mixed sward, a field experiment was conducted with two times and five levels of cattle slurry application. T

A field experiment was conducted to evaluate the effects of cattle compost application on the change of soil physical properties and their relationship to yield performance of selected main forage crops. Maize(CV. Suweon 19) and sorghum hybrids(CV. Pionee

소와 견에 있어서 Unopette가 정자의 형태학적 검사 및 정자농도의 검사를 위하여 사용될 수 있는가를 알아 보기 위하여본 연구를 수행하였다. 소정액 및 견정액을 Unopette에 희석한 후 3-5에 보존하면서 시간경과에 따라서 위상차현미경하에서 관찰하여 다음과 같은 결과를 얻었다. 1. Unopette를 사용하여 관찰한 정자는 48시간까지는 hematoxylin-eosin을 사용하여 정자보다는 높은 정상정자율을 나타내넜다. 2. Unopette를 사