We have investigated some biological characteristics, such as survival rate and fecundity, of Orius laevigatus and Phytoseiulus persimilis, which have been stored at 6, 8, 10, 12±1℃, RH 70±10%, in dark condition. Overall, an appropriate temperature for cold storage was 10℃ for O. laevigatus and 8℃ for P. persimilis. 70% of adult O. laevigatus could survive for 36 days at 10℃. The O. laevigatus stored at 10℃ for 10~50 days laid 37.1～120.5 eggs. Since fresh (no storage) O. laevigatus laid 224.5 eggs, comparative fecundity of stored adults was 16.5~53.7% of normal fecundity. P. persimilis stored at 8℃ for 7~42 days with (food eggs of Tetranychus urticae) laid 11.9～18.9 eggs. Since fresh P. persimilis laid 26.4 eggs, comparative fecundity of stored adults was 45.1~71.6% of normal fecundity.

KNI3126 which is the eco-friendly material made by plant extracts - neem and matrine - have been evaluated for efficacies against 6 main pests and toxicity on natural enemies. Insecticidal efficacies of KNI3126 against plant hopper were above 95% at 5 days after treatment (DAT), whereas pesticidal effects against the cotton aphid were above 95% at 3 DAT and slightly decreased at 5 DAT. Insecticidal efficacy against the palm thrips resulted in lower control value as 68.1% than that of chemical insecticides. KNI3126 showed more than 95% control value against diamond back moth, suggesting that it could suppress the population of pest hard to control as eco-friendly material. Mortalities against two-spotted spider mite were about 80% at 1 DAT and over 90% at 5 DAT, respectively. For evaluation of the toxicity on natural enemies, effect against predatory natural enemy was classified as moderate selective toxicity based on the criterion of International Organization of Bio-Control (IOBC), whereas against parasitic natural enemies was found to be relatively safe. Repellent effect was weak against plant hopper, but strong against two-spotted spider mite. In the toxicity test for safety, KNI3126 was classified as the lowest level at acute oral, acute dermal and fish toxicity test and did not induce the irritancy at skin irritation test and eye irritation test.

With the comparison of native Orius strigicollis (Poppius) to European O. laevigatus (Fieber), we investigated biological characteristics such as developmental period, fecundity, life span, predation ability, and augmentation after release. Experiments were carried out at five temperature condition, 15℃, 20℃, 25℃, 30℃, and 35℃. In the case of O. strigicollis, egg period was 3.1~14.9 days (hatchability: 46.7~88.2%), and developmental period of nymphs was 9.4~42.8 days (survival rate: 2.4~96.5%). Fecundity at each temperature was 28.1, 107.9, 123.6, 127.3, and 18.1, respectively. Life span of adults was 63.4, 40.1, 22.1, 18.3, and 11.0 days, respectively. In the case of O. laevigatus, egg period was 3.1~13.5 days (hatchability: 65.0~89.9%), and developmental period of nymphs was 9.3~42.0 (survival rate: 4.3~80.6%) days. Fecundity at each temperature was 101.8, 218.6, 224.5, 219.5, and 15.7. Life span of adults was 70.6, 66.4, 32.6, 34.5, and 7.3 days, respectively. In long-day condition (16L:8D), fecundity of O. strigicollis and O. laevigatus was 105.8 (18℃)~142.4 (25℃) and 109.5 (18℃)~191.5 (25℃), respectively. In short-day condition (10L:14D), fecundity of them was 1.0 (18℃)~31.8 (25℃) and 63.0 (18℃)~198.8 (25℃), respectively. Daily prey consumption of second instar Frankliniella occidentalis was 14.3 and 10.9, respectively. In greenhouse, density of O. strigicollis begins to increase from mid May and peaked about early July (1.8 individual/flower), while that of O. laevigatus begins to increase from mid March and peaked about early June (6.6 individual/flower).

Many kinds of environment friendly agricultural materials (EFAMs) were used for the plant protection, furthermore they support the growth of crops in the greenhouses and the kindly environment friendly farming. Natural enemies might be used for control of insect pest with EFAMs at the same space and time. For testing the toxicity of EFAMs against to natural enemies, 10 EFAMs were selected and tested in small greenhouses and farm"s fields. In case of Harmonia axyridis larva, there was no predacious activity against cotton aphids in the block with EFAMMo L, EFAMPE D, EFAMME G and EFAMAE A, otherwise, aphid population was rapidly decreased in the control block without EFAMs. Both of Trialeurodes vaporariorum and Encarsia formosa population were slightly decreased in the block with EFAMs. There were significantly decreased in the block with IEFAM C, FEFAM D, EFAMSM Band EFAMPE D compared with control area. Especially, there was significantly maintained a very low population, both T. vaporariorum and E. formosa in the block with EFAMMo C. The parasitized ratio of Aphidius colemani is also very low in the block with EFAMs compared with control area. In the block with IEFAM C, FEFAM D, EFAMPE D and EFAMMo L, there were significantly different with control area in the number of mummies. Otherwise, in case of Diglyphus isaea and Liriomyza trifolii, there was no significantly different between blocks with and without EFAMs. In the block with EFAMMo C, Tetranychus urticae and Phytoseiulus persimilis populations were significantly maintained a very low level. However, the population of T. urticae was increased, but P. persimilis was decreased in the block with EFAMMo L.

This survey was conducted to illustrate insect pests and their natural enemies in soybean fields in Honam area. A total of 23 pest species in 13 families and 13 species of natural enemy were observed during 2002 to 2003. Delia platura, Medythia nigrobilineata, Spodoptera exigua and Aphis glycines occurred relatively with high population densities during the early growth stage of soybean. Dolycoris baccarum, Riptortus clavatus, Nezara antennata, Piezodorus hybneri and Halyomorpha halys began to occur between mid July to early August and lasted until the harvest time. Lepidopteran insect pests, Leguminivora glycinivorella, Pleuroptya ruralis, Scopula missaria, Macdunnoughia purissima and Spodoptera litura occurred significantly in late August in soybean field. Natural enemies of soybean insect pests observed in this survey were seven parasitoids, five predators, and an entomopahogenic fungus. Two egg parasitoid species for R. clavatus, two parasitoid species for P. ruralis, and five natural enemy species (including parasite, predator and entomopathogenic fungi) for either S. litura or A. glycines were identified, respectively. Among them two egg parasitoid species, Gryon japonicum and Ooenyrtus nezarae for R. clavatus, for and one parasitoid species for P. ruralis, that belong to Betbylidae, were new to Korea.

애꽃노린재류(Orius spp.)나 알기생봉류(Trichogramma spp.)와 같은 천적곤충의 대량사육을 위한 대체먹이로서, 국내에서 채집이 가능한 저장곡물해충인 줄알락명나방(Cadra cauteila)의 생물적 특성을 조사하고 대량사육 방법을 개발하였다. 에서 줄알락명나방의 발육기간은 알, 유충, 번데기가 각각 4.2일, 29.8일, 8.3일이었으며, 성충의 수명은 암컷이 5.8일, 수컷이 4.8일이었다. 총산란수는 20, 25, 3에서 각각 128.9개, 207.9개, 139.9개였다. 실험에 사용된 줄알락명나방의 사육사료 모두 발육기간, 우화율, 성충무게 면에서 큰 차이가 없었으나, 대량사육을 위해서는 쌀겨 50%와 병아리사료 50%를 혼합한 사육사료가 비용 면에서 보다 경제적이었다. 줄알락명나방알의 저장조건으로 9에서 7일간 저장이 부화율 82.0%로 4보다 우수하였다. 유충사육케이지 (16249cm)에서 알의 초기접종밀도를 1,000개, 3,000개, 5.000개로 할 때, 우화율은 각각 62.1, 42.4, 29.4%, 성충무게는 각각 9.1, 77, 6.8 mg이었다. 줄알락명나방의 대량사육체계를 \circled1 사료 배합 \circled2알접종 및 유충사육 \circled3번데기 이동 \circled4성충 우화 \circled5알 수확 단계로 구성하고 각각의 과정을 상세히 설명하였다.

우리 나라 골프장의 잔디 가해 해충과 천적에 관한 조사에서 잔디를 가해하는 해충은 6목 10과 28종이 확인되었고, 잔디흑응애(Eriophydae zoysiae)와 고구마뿌리혹선충(Meloidogyne incognita)도 피해를 주고 있었다. 풍뎅이 유충인 굼벵이는 전 조사 골프장의 모든 코스에서 피해를 조고 있었으며, 검거세미나방이나 거세미나방도 많은 피해를 주고 있었다. 그리고 푸충나방류(Crambus sp.)와 깍지벌레류, 각다귀(Tipula sp.)도 잔디에 피해를 주고 있었다. 반면, 일본왕개미(Camponitus japonicus), 곰개비(Formica japonica), 고동털개미(Lasins japonicus)는 직접적인 잔디 피해보다는 간접적인 피해가 많았다. 즉, 잔디나 골프장 군데군데에 집을 만들거나 골퍼들에게 피해를 주고 있었다. 잔디 해충의 천적으로는 곤충병원성 선충과 곤충변원성 곰팡이 및 유화병균등의 곤충 병원미생물과 파리매와 같은 포식성 천적류, 그리고 굼벵이벌 등과 같은 기생성 곤충류가 채집되었다. 병원성 선충으로는 Heterorhabditidae과의 Heterorhabditis sp.와 Steinernematidae과의 Steinernema glaseri와 S. longicaudum이 굼벵이와 잔다밭에서 발견되었다. 특히, 경산의 대구골프장에서 발견된 Heterorhabditia sp,는 동얼룩풍뎅이와 주둥무늬차색풍뎅이 유충에서 분리되었는데 병원성이 매우 뛰어났다. 병원성 곰팡이는 Beauveria bassiana와 Metarhizium anisopliae가 등얼룩풍뎅이에서 확인되었다. 그리고 유화병(milky disease)을 일으키는 Paenibacillus popilliae가 주둥무늬차색풍뎅이(Adoretus tenuimaculatus)와 등얼룩풍뎅이(Exomala orientalis), 주황긴다리풍뎅이(Ectinohoplia rufipes), 녹색풍뎅이(Popillia quadriguttata)에서 발견되었다. 특히, 경산의 대구 골프장에서 발견되 Heterorhabditis sp.는 등얼룩풍뎅이와 주둥무늬차색？뎅이 유층에서 분리되었는데 병원성이 매우 뛰어났다. 병원성 곰팡이는 Beauveria bassiana와 Metarhizium anisopliae가 등얼룩풍뎅이에서 확인되었다. 그리고 유화병(milky disease)을 일으키는 Paenibacillus popilliae가 주둥무늬차색풍뎅이(Adoretus tenuimaculatus)아 등얼룩풍뎅이(Exomala orentalis), 주황긴다리풍뎅이(Ectinohoplia rufipes), 녹색풍뎅이(Popillia quadriguttata)에서 발견되었다. 관찰된 파리매 3종 중 파리매(Promachus yesonicus)의 활동이 가장 활발하였다. 굼벵이벌(Tiphia sp.)은 주둥무늬차색풍뎅이와 등얼룩풍뎅이의 유충에서 확인되었는데 발견된 개체수는 많지 않았다. 식무 기생선충으로는 고구마뿌리혹선충이 들잔디(Zoysia japonica)와 금잔디(Z.matrella), 버뮤다그라스(Cynodon dactylon)에서 발견되었다.