The purpose of this study was to develop Peptoniphilus mikwangii -specific quantitative real-time polymerase chain reaction (qPCR) primers based on the 16S ribosomal RNA (16S rDNA) gene. The specificity of the primers was determined by conventional PCR using 29 strains of 27 oral bacterial species including P. mikwangii. The sensitivity of the primers was determined by qPCR using the purified genomic DNA of P. mikwangii KCOM 1628T (40 ng to 4 fg). The data showed that the qPCR primers (RTB134-F4/RTB134-R4) could detect P. mikwangii strains exclusively and as little as 40 fg of the genomic DNA of P. mikwangii KCOM 1628T. These results suggest that the developed qPCR primer pair can be useful for detecting P. mikwangii in epidemiological studies of oral bacterial infectious diseases.

본 연구는 기장 종자의 발아활력 증진과 입모 균일성을 통한 안정적인 생산을 위해 염수선(비중) 종자정선을 활용한 통한 발아율, 유모의 소질 및 포장 출현율을 알아보고자 수행하였다. 1. 염수선에 따른 기장은 비중이 높아질수록 천립중이 증가 하였으며, 표준발아에서는 정선 종자에서 3품종 모두 91% 이상 발아율을 보였으며, 비중선 1.040 g l-1에서 ‘황금’ 98.9%, ‘이백찰’ 99.4%, ‘황실찰’ 98.9%로 가장 높았다. 2. 모 소질을 조사한 결과 초장에서는 비정선 종자에 비해, 정선된 종자에서 평균 초장은 2.0, 2.2 및 1.9 cm 길었고, 엽장에서는 ‘황금’과 ‘이백찰’에서만 유의성이 있었으며, 경직경과 SPAD에서는 염수선 비중에 따른 유의성이 보이지 않았다. 3. 염수선에 정선된 종자의 평균 근장, 근중 및 경중 모두 길거나 무거웠으며, 근장에서는 1.040 g l-1에서 비정선 종자에 비해 2.9, 3.0 및 2.9 cm 길게 나타났고, 근중과 경중에서도 1.040 g l-1에서 가장 무겁게 조사되었다. 4. 포장 발아활력 검정에서는 품종에 따라 평균출아일수에서만 유의성이 있었으며, 염수선 처리에서는 출아율 및 출아율지수에서 고도의 유의성이 나타났다. 3품종 모두 비중선이 높을수록 출아율과 출아율지수가 증가하였고, 비중선 1.040 g l-1 에서 가장 높은 90.7, 85.3 및 92.8% 출아율이 나타났다. 5. 기장의 출아율 증진과 입모 균일성 향상을 통한 생산성 증대를 위해서는 염수선을 통해 비중선 1.040 g l-1에서의 종자를 선별이 유리한 것으로 사료된다.

Background: Cervical mobilization has been applied mainly for the improvement of arm and neck movements and pain reduction, and little research has been done to improve the executive function. Since this kind of so-called mechanical neck pain is one of most common symptoms, there are controversial issues about this with spine alignment. Posteroanterior (PA) mobilization from the Maitland concept is a process of examination, assessment, and treatment of neuromusculoskeletal disorder by manipulative physical therapy. Objective: To examine the short-term benefits of mobilization for patients with non-specific neck pain. Design: Dual-group Pretest-Posttest Design from the Quasi-Experimental research Methods: Fourteen participants (male 8, female 6; 20’s of their age) with nonspecific neck pains which are distributed all the unilateral or bilateral body side were recruited. Participants were categorized to Neck Pain with Movement Coordination Impairments (NPMCI) and Neck Pain with Mobility Deficits (NPMD) groups according to the results of physical examination. Professional physical therapist who has over 15-years-of clinical experience applicated manipulative therapy for the neck pain, an occupational therapist only conducted evaluations; K-NDI (Korean version of the Neck Disability Index), VAS (Visual Analog Scale), BDS-K (Korean version of Behavioral Dyscontrol Scale) for decreasing possible adverse effects; there were no person who reported other symptoms followed 4 weeks from the trial. Results: In the NPMCI group, data analysis indicated statistical differences between the PA mobilization interventions in NDI and BDS-K; even though, pain was reduced in VAS, this is not a significantly differ. In the NPMD group, data analysis represented statistical differences between the PA mobilization interventions in NDI, VAS and BDS-K; the scores were represented to be increased or the pain got relief. Conclusions: PA mobilization techniques according to Maitland concept have beneficial effects in patients with neck pain and other clinical positive effects which included neck disability, pain itself and motor function of upper extremity.

1. 볼리비아 국토 면적의 34.21%는 농경지로 이 중 89.06% 는 목초지다. 볼리비아 전체인구 중 농촌지역 거주민이 30.7% 인데 반해 차코지역은 과반수(약 58%)가 목축업, 작물재배 등에 종사 중으로 농업이 지역경제의 주요 축을 구성한다. 2. 차코는 생태학적으로 식생이 다양하고 생물다양성이 보전된 곳으로 농업 잠재성은 높은 반면 저개발 되어 있고, 원주민의 한정된 공간에서의 무분별한 재래식 방목과 불충분한 식수와 초지 부족, 기후변화로 토양침식, 생태계 파괴가 진행 되고 있다. 3. 산지축산체계는 나무·관목 및 목초의 선별적 간벌을 통해 임간초지를 조성하고 목초를 생산하며 계획적 방목을 통한 지속가능한 축산관리 모델임이 입증됐다. 4. 체계 도입조건으로 자연·물리적 환경(최소 100ha 이상·수자원 접근 가능), 예산운영(4년간 114,000 Bs 고정지출 능력), 기술적용(가축관리 및 식수 수집·저장), 관리능력(축산관리 기본지식·학습의지)이 주요했다. 5. 농업개발협력 시 발생 문제(예산처리지연, 이해당사자 간 소통 미흡, 참여저조)를 해결하기 위해 지속가능한 사업관리체계 및 거버넌스가 구축돼야 한다.

Leuconostoc spp. are generally utilized as kimchi starters, because these strains are expected to have beneficial effects on kimchi fermentation, including improvement of sensory characteristics. Here, we developed a detection method for verifying the presence of the kimchi starter Leuconostoc mesenteroides WiKim33, which is used for control of kimchi fermentation. A primer set for multiplex polymerase chain reaction was designed based on the nucleotide sequence of the plasmids in strain WiKim33, and their specificity was validated against 45 different strains of Leuconostoc spp. and 30 other strains. Furthermore, the starter strain consistently tested positive, regardless of the presence of other bacterial species in starter kimchi during the fermentation period. Our findings showed that application of a strain-specific primer set for strain WiKim33 presented a rapid, sensitive, and specific method for detection of this kimchi starter strain during natural kimchi fermentation.

미토콘드리아는 세포질 칼슘 항상성 및 ATP 생산에 중요한 역할을 하는 세포 소기관으로 이러한 미토콘드리아의 기능은 성숙과 수정 그리고 배 발달에 매우 중요한 역할을 한다. 미토콘드리아 칼슘 축적은 기능장애를 일으킨다. 그러나 돼지 체외성숙란 및 수정란에서 미토콘드리아 칼슘 변화의 관련성에 관한 연구는 보고된 적이 없다. 본 연구의 목적은 미토콘드리아 칼슘 지시자로 알려진 Rhod-2 염색을 이용하여 성숙란 및 수정란에서 미토콘드리아 칼슘 축적의 변화를 확인하였다. 형태학적 모습의 기준을 통해 난구세포의 세포층과 세포질의 균질도를 바탕으로 G1과 G2로 나누어서 체외성숙을 진행하였다. 이후 두 그룹에서 핵 성숙율을 비교하였을 때, G2가 G1에 비해 낮게 나타났다(p<0.001). 돼지 체외성숙란 및 수정란에서 평균적인 Rhod-2 spot 의 수는 G1보다 G2에서 더 많이 나타났다(6시간째 체외수정란: p<0.05). 다음으로 Rhod-2 spot 수에 따른 난모세포의 비율을 확인하기 위해 Rhod-2 spot 의 수를 4개의 군(n<10, 10≤n<20, 20≤n<30, 그리고 30≤n)으로 나누어 해당 난모세포의 비율을 확인하였다. 체외성숙란 및 체외수정란 모두 G1이 G2에 비해 10개 미만(n<10)인 Rhod-2 spot 의 수를 가지는 난모세포가 많았으며, 체외수정란에서는 유의적으로 높았다(p<0.05). 마지막으로 체외성숙란 및 수정란에서 Rhod-2 intensity 값을 측정하여 두 그룹을 비교하였을 때, G2가 G1에 비해 유의적으로 높은 것을 확인 할 수 있었다(성숙란; p<0.001 그리고 수정란; p<0.05). 본 연구의 결과를 토대로 돼지에서 미성숙 난포란의 형태학적인 품질은 체외성숙 및 체외수정 과정 동안 미토콘드리아 내 칼슘 축적과 관련이 있음을 확인하였다.

With the advent of the digital age, production and distribution of web pages has been exploding. Internet users frequently need to extract specific information they want from these vast web pages. However, it takes lots of time and effort for users to find a specific information in many web pages. While search engines that are commonly used provide users with web pages containing the information they are looking for on the Internet, additional time and efforts are required to find the specific information among extensive search results. Therefore, it is necessary to develop algorithms that can automatically extract specific information in web pages. Every year, thousands of international conference are held all over the world. Each international conference has a website and provides general information for the conference such as the date of the event, the venue, greeting, the abstract submission deadline for a paper, the date of the registration, etc. It is not easy for researchers to catch the abstract submission deadline quickly because it is displayed in various formats from conference to conference and frequently updated. This study focuses on the issue of extracting abstract submission deadlines from International conference websites. In this study, we use three machine learning models such as SVM, decision trees, and artificial neural network to develop algorithms to extract an abstract submission deadline in an international conference website. Performances of the suggested algorithms are evaluated using 2,200 conference websites.

Korean Native Pig (KNP) has a uniform black coat color, excellent meat quality, white colored fat, solid fat structure and good marbling. However, its growth performance is low, while the western origin Yorkshire pig has high growth performance. To take advantage of the unique performance of the two pig breeds, we raised crossbreeds (KNP × Yorkshire to make use of the heterotic effect. We then analyzed the liver transcriptome as it plays an important role in fat metabolism. We sampled at two stages: 10 weeks and at 26 weeks. The stages were chosen to correspond to the change in feeding system. A total of 16 pigs (8 from each stage) were sampled and RNA sequencing was performed. The reads were mapped to the reference genome and differential expression analysis was performed with edgeR package. A total of 324 genes were found to be significantly differentially expressed (|log2FC| > 1 & q < 0.01), out of which 180 genes were up-regulated and 144 genes were down-regulated. Principal Component Analysis (PCA) showed that the samples clustered according to stages. Functional annotation of significant DEGs (differentially expressed genes) showed that GO terms such as DNA replication, cell division, protein phosphorylation, regulation of signal transduction by p53 class mediator, ribosome, focal adhesion, DNA helicase activity, protein kinase activity etc. were enriched. KEGG pathway analysis showed that the DEGs functioned in cell cycle, Ras signaling pathway, p53 signaling pathway, MAPK signaling pathway etc. Twenty-nine transcripts were also part of the DEGs, these were predominantly Cys2His2-like fold group (C2H2) family of zinc fingers. A protein-protein interaction (PPI) network analysis showed that there were three highly interconnected clusters, suggesting an enrichment of genes with similar biological function. This study presents the first report of liver tissue specific gene regulation in a cross-bred Korean pig.

High surface carbon aerogels with hierarchical and tunable pore structure were prepared using ionic liquid as carbon precursor via a simple salt templating method. The as-prepared carbon aerogels were characterized by nitrogen sorption measurement and scanning electron microscopy. Through instant visual observation experiments, it was found that salt eutectics not only serve as solvents, porogens, and templates, but also play an important role of foaming agents in the preparation of carbon aerogels. When the pyrolyzing temperature rises from 800 to 1000°C, the higher temperature deepens the carbonization reaction further to form a nanoporous interconnected fractal structure and increase the contribution of super-micropores and small mesopores and improve the specific surface area and pore volume, while having few effects on the macropores. As the mass ratio of ionic liquid to salt eutectics drops from 55% to 15%, that is, the content of salt eutectics increases, the salt eutectics gradually aggregate from ion pairs, to clusters with minimal free energy, and finally to a continuous salt phase, leading to the formation of micropores, uniform mesopores, and macropores, respectively; these processes cause BET specific surface area initially to increase but subsequently to decrease. With the mass ratio of ionic liquids to salts at 35% and carbonization temperature at 900°C, the specific surface area of the resultant carbon aerogels reached 2309 m2 g–1. By controlling the carbonization temperature and mass ratio of the raw materials, the hierarchically porous architecture of carbon aerogels can be tuned; this advantage will promote their use in the fields of electrodes and adsorption.

고리매개등온증폭법(LAMP)으로 흰등멸구를 특이적으로 구별해낼 수 있는 프라이머 세트(WBPH-65)가 핵내 ITS2영역의 전체염기서 열(KC417469.1)을 바탕으로 설계 제작되었다. WBPH-65는 총 6개의 프라이머, F3 (18 bp), B3 (18 bp), FIP (43 bp), BIP (40 bp), LF (21 bp), LB (25 bp)로 구성되는데, 전체 합한 길이가 165 bp이다. WBPH-65를 흰등멸구, 벼멸구 및 애멸구의 게놈 DNA와 65℃에서 60분간 고리 매개등온증폭 반응시켰을 때, 흰등멸구 시료에서만 증폭 산물들이 관찰되었다. 65℃에서 WBPH-65와 흰등멸구 게놈 DNA의 양과 반응시간을 달리하여 형광반응을 관찰하였을 때 40분 반응에서는 10과 100 ng DNA에서, 60분 반응에서는 0.01, 0.1, 1, 10, 100 ng DNA에서 발광여부 가 명확히 구별되었다. 그러나 20분과 30분 반응에서는 준비된 모든 DNA 양에서 발광여부 구별이 어려웠다. 한편, WBPH-65에서 LF와 BF 프라이머를 뺀 경우 60분 반응에서는 벼멸구, 애멸구 뿐만 아니라 흰등멸구의 게놈 DNA에서도 발광되지 않았다. 본 연구 결과로부터 WBPH-65가 60분 이내 반응에서 흰등멸구를 특이적으로 구별하기 위해서는 6개의 프라이머가 모두 필요하며 최소한 벼멸구와 애멸구를 구별 해낼 수 있음을 확인하였다.