탈피호르몬의 작용을 방해하는 농약제인 tebufenozide 를 알에서 부화한 지 7일째의 C. riparius에 처리하여 성충이 될 때까지의 생장과정을 연구하였다. C. riparius 에 control, 10 μg L-1, 30 μg L-1, 60 μg L-1와 100 μg L-1의 농도로 약제를 처리하였다. 전체적으로 약제를 처리한 하루 후인 D12에 16%가 치사

사장교에서 케이블은 교량 전체에 있어서 매우 중요한 요소이다. 차량, 바람 혹은 풍우에 의한 케이블의 진동은 교량의 안전성과 사용성을 감소시키는 주요 원인이 되어왔으며 이러한 문제를 해결하는 효과적인 방법중의 하나는 케이블 댐퍼를 설치하는 것이다. 이 케이블 댐퍼를 최적으로 설계하기 위해서는 케이블의 동특성을 정확하게 평가해야 하며 케이블 동특성치를 얻기 위해서는 정확한 가진이 필요하다. 따라서 본 연구에서는 케이블 가진시스템을 개발하고 성능을 평가하기 위해 케이블 가진시스템의 운동방정식을 유도하였으며, 케이블 가진기를 케이블 모형에 설치하여 정현진동실험과 공진진동실험을 수행하여 케이블의 동특성을 효과적으로 구하였다.

Human Prourokinase (proUK) offers potential as a novel agent with improved fibrin specificity and, as such, may offer advantages as an attractive alternative to urokinase that is associated with clinical benefits in patients with acute peripheral arterial occlusion. For production of transgenic cow as human proUK bioreacotor, we conducted this study to establish efficient production system for bovine transgenic embryos by somatic cell nuclear transfer (NT) using human prourokinase gene transfected donor cell. An expression plasmid for human prourokinase was constructed by inserting a bovine beta-casein promoter, a green fluorescent protein (GFP) marker gene, and human prourokinase target gene into a pcDNA3 plasmid. Cumulus cells were used as donor cell and transfected with the expression plasmid using the Fugene 6 as a carrier. To increase the efficiency for the production of transgenic NT, development rates were compared between non-transfected and transfected cell in experiment 1, and in experiment 2, development rates were compared according to level of GFP expression in donor cells. In experiment 1, development rates of non-transgenic NT embryos were significantly higher than transgenic NT embryos (43.3 vs. 28.4％). In experiment 2, there were no significant differences in fusion rates (85.4 vs. 78.9％) and cleavage rates (78.7 vs. 84.4％) between low and high expressed cells. However, development rates to blastocyst were higher in low expressed cells (17.0 vs. 33.3％), and GFP expression rates in blastocyst were higher in high expressed cells (75.0 vs. 43.3％), significantly.

N-nitrosoethylurea(NEU)에 의한 지질 과산화물의 함량 변화와 알데히드 대사효소 및 항산화효소의 활성변화를 쥐 간세포에서 측정하였다. 알데히드 대사효소로는 alcohol dehydrogenase와 aldehyde dehydrogenase가 사용되었고 항산화효소로는 glutathione transferase, superoxide dismutase, glutathione reductase와 catalase가 사용되었다. 쥐 간세포에 다양한

Agarooligosaccharides were produced by β-agarase from Bacillus cereus ASK 202. LD_(50) of Agarooligosaccharides was determined to be 1359 mg/kg which corresponded to GRAS material. Agarooligosaccharides at 5% level exhibited 88.3% inhibition on TA98 and 54% on TA100, indicating agarooligosaccharides to be potent antimutagenic substance. Immunologic activity of agarooligosaccharides was also confirmed by mouse spleen cell culture. Agarooligosaccharides addition of 200 ㎕/ml stabilized spleen cells (2.5 × 10^6 cells/ml) as compared to control (6.4 × 10⁴ cells/ml).

Agar, one of the most abundant marine products has not been utilized extensively because of low level of processing technology in Korea. This research was carried out to improve the utilization of agar and consequent increase in profit. Antibacterial activity of agarooligosaccharides were evaluated against bacteria causing putrefaction and food poisoning. Addition of 0.4% agarooligosaccharides showed antibacterial activity toward Staphylococcus aureus and Escherichia coli O157:H7; furthermore, autoclave treatment of agarooligosaccharides solution enhanced the antibacterial activity. Agarooligosaccharides showed high stability against the pH change. Addition of amino acid(alanine, lysine, glycine, phenylalanine) in agarooligosaccharides solution enhanced antibacterial activity in E. coli O157:H7, Streptococcus mutans and Staphylococcus aureus.

옥수수에 5㎍/g의 FB_1을 인위적으로 첨가한 후 co-rotating mixing twin screw extruder를 사용하여 140, 160, 및 180℃에서 각각 screw speed를 80, 100 및 120rpm으로 조정하여 extrusion 처리하였다. Extrusion 처리에 의한 FB_1 감소율은 약 41∼45% 수준이었으며, 처리 조건에 따른 차이는 없었다. Glucose. fructose 및 sucrose를 각각 첨가한 옥수수를 140℃. 120rpm에서 extrusion 처리하였을 때 glucose를 첨가한 옥수수에서의 FB_1 감소율이 증가하였다. Glucose 함량의 증가에 따라 FB_1의 감소율도 증가하였으며, glucose가 2.5%, 5% 및 7.5% 존재할 때 extrusion 처리 옥수수에 잔존하는 FB_1은 각각 51%. 34% 및 19% 수준이었다. 본 연구는 한국과학재단 1998년도 전반기 해외 Post-doc. 연수지원사업에 의하여 수행된 연구결과의 일부로서 이에 깊이 감사드립니다.

경간사이에 creep-coupler가 설치된 경부 고속철도 교량에 TGV-K 열차의 제동에 의한 교량의 종방향 동적거동을 해석하였다. 교량은 40m 길이의 2경간 연속교이며, 종방향 충격 하중을 인접 경간 혹은 교대로 전달하기 위한 목적으로 인접하고 있는 두 교량 사이의 creep-coupler가 설치되었다. 철도교의 경우에는 레일에 대한 종방향 축력검토가 매우 중요하므로, 이를 지지하고 있는 교량의 하부구조(교각과 기초)의영 향을 고려한 교량의 동적거동해석이 요구된다. 본 연구에서는 TGV-K의 실제 제동하중에 의한 KHSR(Korea high speed railway)에 건설중인 실제교량의 동해석을 하부구조와 동특성치를 고려하여 수행하였다. TGV-K는 객차사이에 대차가 위치하므로 전체 열차의 모델링이 한꺼번에 이루어 져야한다. 동핵석을 위해서 열차의 3차원 수치모델링이 이루어졌다. TGV-K의 제동은 동력차의 전기적인 제동에 의한 회생제동력(regenerative braking force)과 객착의 기계적인 판제동(disk braking)으로 이루어진다. 이러한 제동작용의 고려에 실제 TGV-K의 제동함수가 사용되었다.

the present study was carried out to develop a completely defined culture system and determine if high NaCl concentrations in defined (PVA added) or semi-defined (BSA added) medium is toxic to bovine embryos. Oocytes from slaughterhouse ovaries were matured and fertilized in vitro. After 30 h of insemination, only 2-cell stage embryos were selected and cultured for this experiment. The culture media used were as follows : TLP(114 mM of NaCl) + BSA (3 mg/ml), TLP + PVA (1 mg/ml), mTLP(96 mM of NaCl) + BSA, mTLP + PVA. Six to ten embryos were placed into a 301 drop of each medium and the embryos were examined at 10 day post-insemination without medium renewal. The experiment was replicated 4 times. All data were analyzed by chi-square. There were no significant differences among TLP-BSA, mTLP-BSA and mTLP-PVA in blastocyst development (21.6, 17.2 and 20.2%), respectively. Also, no differences were obtained in hatching rates (11.7, 9.9 and 12.2%), respecitively. However, there were significant differences between TLP-PVA (1.7% and 0.6%) and other group in blastocyst formation and hatching rates, respectively (p<0.01). Development of in vitro produced embryos cultured in BSA containing medium was not affected by high NaCl concentration, but in the completely defined medium, embryonic development was highly affected by NaCl. This study shows that reduced NaCl concentration in completely defined medium is beneficial for development of bovine pre-implantation embryos in vitro.

The effect of natural foods, utilizing the extracts or juices of teas(Green tea; Camellia sinesis, Du' thong; Eucommia ulmoides Oliver), medicinal plants(Eu sung cho; Houttuynia cordata Thunb, Sam back cho; Saurus Chinensis, Baek hwa sa seal cho; Oldenladia diffusa Roxb.) seaweeds(Laver; Porphyra tenera, Sea mustard; Undaria pinnatifida, Sea staghorn; Condium fragile) and vegetables(Sweet pepper; Capsicum annuum var. angulosum, Kale; Brassier oleracea var. acephala, Cucumber; Cucumis satiaus, Onion; Album cepa) and fruits(Tomato; Lycopericon esculentum, Maesil; Prunus mume, Plum; Prunus salicina and Grape; Vitis sppJon the inhibition of N-Nitrosodimethylamine(NDMA) formation was investigated from the various conditions. The inhibition effect was observed in vitro using the reaction fluids of pH 1.2, 4.2 and 6.0. )mom the teas and medicinal plants, there was a positive response of NDMA formation; however, from the seaweed extracts, there was a negative response of the inhibition effect of NDMA formation, and as the pH of reaction fluids and the amount of materials increase, the inhibition of NDMA formation was strengthened. The inhibition ratios by the level of pH are as follows: under pH 1.2 vegetable juice were 57.6 99.7% and fruits were 35.9 99.7%; under pH 4.2 vegetable juice were 55.0-97.5% and fruits were 21.3 96.8%. All of the materials observed has been proved and shown the inhibition effect of NDMA formation.

고추장에서 점물질을 생산하는 세균을 분리하고 Bacillus licheniformis로 동정하였다. 본 균주는 sucrose 6%, Yeast extract 0.1%, peptone 0.1%, NaC1 3%의 배지조성에서 pH를 6으로 조정하였을 때 점물질 생산량이 가장 높았고 생산량은 34mg/250ml였다. 이 점물질은 1%농도에서 필름 형성능이 있었고, 0.25-1.00%의 시험범위에서 Kaolin을 침전시키고, 유산균 현탁액을 안정화시키는 능력을

본 논문에서는 다지지점 가진에 의한 구조물의 동적응답을 구하기 위하여 유한요소모형을 수정하고 등가하중을 도입하는 간단한 기법을 제안하였다. 제안방법은 다지지점 입력에 대한 해석기능이 있는 기존의 범용구조해석 S/W를 이용한 선형 및 비선형 해석결과와 비교하여 검증하였다. 이 기법은 다지지점 입력에 대한 해석기능이 없는 범용유한요소해석 S/W 및 비선형 구조해석을 위하여 특별히 개발되었던 전산프로그램을 이용하여 다지지점 입력에 대한 지진응답해석을 수행할 때 유용하게 활용될 수 있을 것으로 기대된다.

In previous reports, the authors isolated the algicidal marine bacterium, Alteromonas sp. SR-14 and demonstrated its growth inhibition of diatom, Chaetoceros calcitrans (C. calcitrans). In this paper, we studied the effects of cell free culture filtrate of Alteromonas sp. SR-14 on the growth of C. calcitrans, and the characteristics of the algal growth inhibition substance. The culture filtrate of Alteromonas sp. SR-14 grown in peptone broth showed growth inhibition activity against C. calcitrans. The reasonable culture conditions of the bacterium for producing of algal growth inhibition substances were 15-20℃ in temperature, 7.0-9.0 in pH and 23-30‰ in salinity, respectively. The algal growth inhibition activity of culture filtrate was increased from stationary phase in growth curve of Alteromonas sp. SR-14. The molecular weights of algal growth inhibition substances produced by Alteromonas sp. SR-14 were ranged about from 3 KDa to 12 KDa. Among the substances, less than 10 KDa fraction were stable by heating at 100℃ for 10 minutes, while more than 10 KDa fraction were heat labile. According to the experimental results, the algal growth inhibition substance produced by the bacterium was not a single compound.

본 연구는 두 지점간의 진동수 응답함수를 이용하여 시간 및 진동수 영역에서의 원심펌프(20Hp. 50Hp) 가진력을 추정하기 위한 것이다. 진동수 응답함수는 실수부와 허수부에 대한 신호정보를 가지고 있으며 응답함수 역시 실수부와 허수부에 대한 신호정보를 가지고 있다 따라서 이들 진동수 응답함수 및 응답함숴의 복소수 계산으로부터 가진력을 실험적으로 구하였으며 이론적인 방법에 의하여 구한 가진력과 실험적으로 구한 값을 비교함으로서 펌프에 의한 주진동수 성분의 크기는 펌프 및 모터중량의 10-25% 정도가 됨을 제시하였다. 가진력 산정을 위한 시간영역에서의 불평형질량의 크기는 펌프 및 모터중량의 약 30-60%임을 알수 있었다 한편 펌프회전에 의한 진동은 주진동수 이외에도 주진동수의 2-3배의 크기를 갖는 성분이 있음을 알 수 있었으며 대상 콘크리트 슬래브의 고유진동수와의 가진진도수비를 달리함에 따라 정확한 진동전달률을 조절하는데 활용할 수 있다.

The purpose of this study was to examine the increased bone loss caused by ovariectomy through monitoring the concentrations of the collagen and the pyridinoline crosslinks of collagen. The ovariectomized rats treated for 8 weeks, were divided at random into two or three groups of 10. Ovariectomies were carried out from the saline-treated group (Ovx), the estrogentreated group (Ovx+ES) and chondroitin sulfate-treated group (Ovx+CS). Sham operations were performed on the sham-operated group (Sham). Ovx+ES and Ovx+CS groups showed the remarkably increased collagen and pyridinoline amount in the bone and cartilage compared to Ovx group. And as the result of the measurement of SOD, Catalase and GPx which are antioxidant enzyme, SOD and Catalase activities in Ovx group were much higer than in Sham group. But they were significantly decreased in Ovx+CS group. Based on these results, it is supposed that estrogen and condroitin sulfate can enhance collagen synthesis and affect the pyridinoline formation in collagen fibrils through stimulating lysyl oxidase activity. And it is also thought that chondroitin sulfate can inhibit aging by reducing antioxidant enzyme.