Inonotus obliquus is one of the immune-regulatory substances and is recognized to play the role in the metabolic process of inflammation, allergy and immuntiy. The purpose of this study was to evaluate the effects of water extracts of Inonotus obliquus (IOW) on the liver lymphocyte immune function in the Sprague-Dawley male rats treated with carbon tetrachloride (CCl4) to induce liver damage. Rats were fed with each experimental diet and water for 4 weeks. We found that effects of IOW on interferon-gamma (IFN-γ), signal transducer and activator of transcription 1 (STAT1), phospho-signal transducer and activator of transcription 1 (pSTAT1) and GATA-binding protein 3 (GATA-3) were decrease in vivo. Interleukin-4 (IL-4), STAT6, pSTAT6 and T-box expressed in T-cells (T-bet) decreased significantly lower in CCl4+IOW group than the CCl4 group. Our data indicated that cytokine protein production were increased in CCl4 group and CCl4+IOW group. As a result of this study, we assume that IOW fed could regulate the immuno-modulating functions through regulate the cytokine production capacity activated by liver damage.

콩 재배에서 가장 큰 문제의 하나인 도복의 경감을 위한 재배법 개선 방안과 초관 높이 차이에 의한 광합성능력의 향상을 모색하고자 열간 교호 적심이 콩의 생육과 수량에 미치는 영향을 분석한 결과는 다음과 같다. 1. 초관 높이 차이는 무적심 1열+적심 1열에서 가장 컸으며, 도복은 무적심 1열+적심 1열에서 도복경감 효과가 가장 컸다. 2. 제 9본엽의 광합성능력은 무적심 1열+적심 1열의 무적심 열이 가장 높았다. 3. 엽면적지수는 8월 17일 이후에 무적심 열에 비하여 적심 열이 높았고, 9월 5일에는 무적심 1열+적심 1열의 엽면적지수가 가장 높았다. 4. 수량은 무적심 1열+적심 1열에서 주경 상반부의 입수증가와 입중 증대로 전체 무적심에 비하여 14~% , 전체 적심에 비하여 2~% 증수되었다.

In order to examine if arsenic, one of environmental stresses, contributes to hypertension as one of cardiovascular pathological factors, this study was performed in vivo and in vitro, using intacted or pithed rats and aorta ring preparation, respectively. And also the relationship between expression of heat shock protein (HSP) 90 and vasoactives-induced contractile response was elucidated. To measure blood pressure, the carotid arterial pressure was recorded on physiograph(Grass Co. 79E) connected to strain gauge. On the other hand, contractile response of vascular ring preparation isolated from rat was determined in organ bath and was recorded on physiograph connected to isometric transducer. And HSP was detacted by Western blotting whole cell lysis. Preganglionic nerve stimulation was increased by 26.0% in arterial pressure of rat treated with arsenic. Vascular contractile response was monitored and HSP were measured by Western blotting of whole lysis prepared from samples exposed with 0, 0.5, 1, 2 and 4 mM of arsenic for 8 hours. The dose-vascular responses of potassium chloride were augmented by increasing dose of arsenic in the strips exposed to arsenic for 8 hours, and were not augmented for 1, 3, 5 hours. And the response of relaxation of rat aorta induced by histamine was not influenced by arsenic stress. The increase of HSP 90 expression in rat aorta was pronounced at 8 hours after 4 mM of arsenic treatment, but HSP 60 expression was not. Arsenic stress not only increased the expression of HSP 90 in the rat aorta, but also augmented contractions to potassium chloride. These results indicated that arsenic stress was sufficient to induce heat shock protein 90, resulting in increased vascular contractility in rat aorta.