The present study was conducted to investigate effects of rabbit meat extract on energy metabolism and muscle differentiation in C2C12 myotubes. Water extract of rabbit meat (10, 50, 100, and 200 μg/ml) was used to treat differentiated C2C12 cells. Reverse transcriptase polymerase chain reaction (RT-PCR) and western blot analysis were used to determine mRNA or protein levels of energy metabolism-related genes. Total adenosine triphosphate (ATP) content was also measured. Treatment with rabbit meat extract significantly increased expression levels of muscle differentiation markers (myogenin and myosin heavy chain) and mitochondrial biogenesis regulators (PGC1α, NRF1, and TFAM) in C2C12 myotubes compared to non-treated control. Additionally, rabbit meat extract activated phosphorylation of AMPK and acetyl-coA carboxylase (ACC). Rabbit meat extract significantly increased ATP contents in myotubes. These results suggest that rabbit meat extract has the potential to improve energy metabolism in skeletal muscles.

Membrane potential is based on the difference in electric potential between membrane that is induced by the concentration differences. This study focused on membrane synthesis for ultra-thin ion exchange membrane. Using those membranes, prototype concentration cell were produced. Interestingly, those concentration cells responds to all external changes, for example, vibration, bending, temperatures. We expect that the concentration cells could extract the useful energy from any type of low-degree energies.

Living organisms are based on the mass flux that induces various biological reactions using nanosized asymmetric ion channels as highly selectivity. However, it is the aggressive challenge to use these channels for artificial membranes because these are fragile and susceptible to deterioration under pH, temperature, mechanical stress, etc. This study, for the first time, synthesized a virus nanochannel-embedded thin film composite membrane. This membrane shows the extremely high ion selectively as well as low resistance. This property can be used to extract various multi-energies, for example, external pressure, water dropping, temperature difference, etc. This electricity results from the entropy gradient between membrane like electric eels.

In the earlier papers we analyzed the axisymmetric, nonstationary electrodynamics of the central black hole and a surrounding thin accretion disk in an active galactic nucleus. Based on those papers we analyze the axisymmetric, nonstationary force-free black hole magnetosphere and the motion of the plasma. We concentrate on deriving the totally new equations for the entropy incrrease and the angular momentum, energy extraction of the black hole.

This study was conducted to evaluate Raphanus sativus extracts to methane reduction in rumen. Five different levels of R. sativus extracts were used to investigate the most effective dosing level for the decrease of methane production in the rumen. The rumen fluid was collected from a cannulated one Hanwoo cow (BW=450±30 kg) consuming 600 g/kg timothy and 400 g/kg concentrate. On fermentation day, rumen fluid was collected at 2 hr postfeeding R. sativus extracts was dosed to achieve final concentration of 0, 1, 3, 5, 7, and 9% respectively, to fermentation bottles containing the mixture of rumen fluid and McDougall’s buffer and 300 mg of timothy was added as a substrate. The fermentation was conducted for 3, 6, 9, 12, 24, 48 and 72 hr incubation time at 39℃ with shaking. In vitro ruminal pH values were measured normal range for ruminal fermentation. Dry matter disappearance was significantly higher (p<0.05) at 3 hr incubation time 1, 3 and 5% doses than that of control. The highest methane reduction was observed in 12 hr incubation time 5, 7 and 9%. The carbon dioxide emission was also significantly (p<0.05) lower than that of control at 12 hr incubation time 5, 7 and 9%. The total volatile fatty acid was no significant difference between control and all doses level at 12 and 24 hr incubation time. At 24 hr incubation time, the result of real-time PCR were indicated that M. archea was significantly lower (p<0.05) at all doses level comparing to that of control. In conclusion, R. sativus extracts were significantly decreased methane emission. R. sativus extracts were significantly lower (p<0.05) than that of control at 12 hr incubation time 5, 7 and 9% and no adversely effect in rumen pH, dry matter disappearance and total VFA.

우리나라는 태권도의 종주국임에도 불구하고 신체활동량과 그에 따른 에너지 소모량에 대한 기초연구가 미흡하며 이에 대한 연구가 시급한 상태이다. 본 연구에서는 센서(SenseWear® PRO2 Armband)를 이용하여 태권도의 신체활동 량을 획득하였고, Inbody 520을 통하여 체성분 데이터를 획득 하였으며, CT로 하체부위(허벅지)를 스캔하여 허벅지 근육면적 데이터를 추출하였다. 획득된 데이터를 이용하여 허벅지 둘레와 근육면적의 상관관계, 허벅지 근육면적과 분 당 에너지 소모량의 상관관계를 분석하였다. 분석결과 실험자 8명의 평균 허벅지 근육면적은 132.79 cm2 였으며, 무 릎뼈(Pattela) 위 20 cm의 평균 허벅지 전체 면적은 178.79 cm2이었다. 실험자들의 허벅지 둘레와 근육면적은 상관 관계가 있는 것으로 나타났다. 실험자들의 평균 분당 에너지 소모량은 6.94 칼로리였으며, 허벅지 근육면적과 평균 분 당 에너지 소모량도 상관관계가 있는 것으로 나타났다.