Photosynthesis and respiration of seagrasses are mainly controlled by water temperature. In this study, the photosynthetic physiology and respiratory changes of the Asian surfgrass Phyllospadix japonicus, which is mainly distributed on the eastern and southern coasts of Korea, were investigated in response to changing water temperature (5, 10, 15, 20, 25, and 30°C) by conducting mesocosm experiments. Photosynthetic parameters (maximum photosynthetic rate, Pmax; compensation irradiance, Ic; and saturation irradiance, Ik) and respiration rate of surfgrass increased with rising water temperature, whereas photosynthetic efficiency (α) was fairly constant among the water temperature conditions. The Pmax and Ik dramatically decreased under the highest water temperature condition (30°C), whereas the Ic and respiration rate increased continuously with the increasing water temperature. Ratios of maximum photosynthetic rates to respiration rates (Pmax : R ) were highest at 5°C and declined markedly at higher temperatures with the lowest ratio at 30°C. The minimum requirement of Hsat (the daily period of irradiancesaturated photosynthesis) of P. japonicus was 2.5 hours at 5°C and 10.6 hours at 30°C for the positive carbon balance. Because longer Hsat was required for the positive carbon balance of P. japonicus under the increased water temperature, the rising water temperature should have negatively affected the growth, distribution, and survival of P. japonicus on the coast of Korea. Since the temperature in the temperate coastal waters is rising gradually due to global warming, the results of this study could provide insights into surfgrass responses to future severe sea warming and light attenuation.

As aeration is an energy-intensive process, its control has become more important to save energy and to meet strict effluent limits. In this study, predictive aeration control based on the respirometric method has been applied to the sequencing batch reactor (SBR) process. The variation of the respiration rate by nitrification was great and obvious, so it could be a very useful parameter for the predictive aeration control. The maximum respiration rate due to nitrification was about 60 mg O2/L‧h and the maximum specific nitrification rate was about 7.5 mg N/g MLVSS‧h. The aeration time of the following cycle of the SBR was daily adjusted in proportion to that which was previously determined based on the sudden decrease of respiration rate at the end of nitrification in the respirometer. The aeration time required for nitrification could be effectively predicted and it was closely related to influent nitrogen loadings. By the predictive aeration control the aerobic period of the SBR has been optimized, and energy saving and enhanced nitrogen removal could be obtained.

The respirometric technique has been used to analyze the nitrification process in a sequencing batch reactor(SBR) treating municipal wastewater. Especially the profile of the respiration rate very well expressed the reaction characteristics of nitrification. As the nitrification process required a significant amount of oxygen for nitrogen oxidation, the respiration rate due to nitrification was high. The maximum nitrification respiration rate, which was about 50 mg O2/L･h under the period of sufficient nitrification, was related directly to the nitrification reaction rate and showed the nitrifiers activity. The growth rate of nitrifiers is the most critical parameter in the design of the biological nutrient removal systems. On the basis of nitrification kinetics, the maximum specific growth rate of nitrifiers in the SBR was estimated as 0.91 d-1 at 20℃, and the active biomass of nitrifiers was calculated as 23 mg VSS/L and it was about 2% of total biomass.

As the sequencing batch reactor process is a time-oriented system, it has advantages of the flexibility in operation for the biological nutrient removal. Because the sequencing batch reactor is operated in a batch system, respiration rate is more sensitive and obvious than in a continuous system. The variation of respiration rate in the process well represented the characteristics of biological reactions, especially nitrification. The respiration rate dropped rapidly and greatly with the completion of nitrification, and the maximum respiration rate of nitrification showed the activity of nitrifiers. This study suggested a strategy to control the aeration of the sequencing batch reactor based on respirometry. Aeration time of the optimal aerobic period required for nitrification was daily adjusted according to the dynamics of respiration rate. The aeration time was mainly correlated with influent nitrogen loadings. The anoxic period was extended through aeration control facilitating a longer endogenous denitrification reaction time. By respirometric aeration control in the sequencing batch reactor, energy saving and process performance improvement could be achieved.

본 논문은 깊이 카메라(Creative Senz3D)를 이용하여 호흡률을 측정하는 것에 대한 정확도와 영향을 미치는 요인들 을 분석하였다. 영향 요인 분석에서는 깊이 카메라가 가지는 깊이 값에 대한 오차와 노이즈 그리고 주위 조도의 영향에 대하여 실험 연구를 진행하였다. 그 결과 깊이 카메라와 측정 대상의 거리가 증가함에 따라 깊이 값의 오차가 증가하였 고 깊이 영상의 오른쪽은 실제 거리보다 깊이 값이 크게 측정되고 왼쪽은 실제 거리보다 깊이 값이 작게 측정되었다. 이에 따라 깊이 값이 영상의 영역에 따라 비대칭성을 가지고 있음을 알 수 있었다. 깊이 카메라와 측정 대상의 각도가 틀어짐에 따라서도 깊이 값의 차의 오차가 증가하였으며 깊이 카메라의 노이즈는 측정 거리가 멀어짐에 따라 점점 증가하였고 노이즈를 측정하는 윈도우의 크기가 증가함에 따라 감소하였다. 주위 조도는 깊이 값에 영향을 주지 않았다. 또한 실제 상황에서 사람을 대상으로 20회 호흡을 하게 하여 깊이 카메라를 이용해 호흡률을 측정하였고 호흡률이 제대로 측정됨을 확인하였다.

Oxygen consumption is a useful parameter for evaluating mammalian embryo quality, since individual bovine embryos was noninvasively quantified by scanning electrochemical microscopy (SECM). Recently, several approaches have been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygen consumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, we measured to investigate the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo using a SECM. In addition to, the expression of pluripotent gene and anti-oxidant enzyme was determined using real-time PCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygen consumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos, indicating that oxygen consumption reflects the embryo quality (Grade I). Oxygen consumption of blastocyst was measured using a SECM and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts (10.2 × 1015/mols—1 versus 6.4 × 1015/mols—1, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7 and 110.2 in the oxygen consumption of below 10.0, 10.0∼12.0 and over 12.0∼1015/mols—1, respectively. Pregnant rate in recipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0∼12.0 and over 12.0 × 1015/mols—1, respectively. GPX1 and SOD1 were significantly increased in over —10.0 group than below 10.0 groups but in catalase gene, there was no significant difference. On the other hand, In OCT-4 and Sox2, pluripotent gene, there was a significant difference (p<0.05) between the below-10.0 (0.98 ± 0.1) and over 10.0 (1.79 ± 0.2). In conclusion, these results suggest that measurement of oxygen consumption maybe help increase the pregnant rate of Hanwoo embryos.

Breaker 단계 토마토의 저온장해 증상은 착색불량과 호흡률 증가 등으로 알려져 있다. 본 실험은 이러한 저온 장해 증상과 맞물려 나타나는 이산화탄소 발생 증가와의 관계를 분석하고, 나아가 저장 중 원예산물 저온장해의 판단기준으로 이용할 수 있는 기초 자료를 만들고자 실시하였다. Breaker 단계로 선별된 토마토는 2℃에서 1차로 0, 3, 6, 9일간 2차로 0, 1, 2, 3, 10일간 저장 후 상온으로 옮겨 3, 6, 9, 12, 24, 36, 48시간마다 호흡률을 측정하였다. 저온장해로 인한 토마토 과실의 호흡률은 1일 저장 처리구부터 증가하였으며 저온 저장기간이 길어질수록 발생량 증가폭도 커졌다. Breaker 단계 토마토의 과피색은 a* 값으로 나타내었는데 각 기간별로 저온저장 후 상온으로 옮겨 10일째에 측정하였는데 Breaker 단계 토마토는 2일 저장 처리구부터 a* 값이 증가가 둔화되면서 착색불량이 나타났다. 저온저장 기간에 증가함에 따라 증가한 호흡률과 반대로 감소한 a* 값의 상관계수(r)는 0.9716로 1%의 유의성을 나타내었다 이상의 결과로 보아 Breaker 단계 토마토의 경우 저온 저장후 상온으로 옮긴 직후 이산화탄소 발생 양상으로 저온장해를 미리 진단할 수 있었으며, 그 발생량 증가폭으로 저온장해 정도도 파악할 수 있을 것으로 생각된다.

병결점 부근의 저장온도에 따른 수삼의 내적 품질 변화 연구로 수삼을 각각 , 및 에서 16주간 저장하면서 저장온도에 따른 호흡률, 경도, 가용성 고형물함량, 전분함량, pH, 사포닌함량과 관능적 품질의 변화를 조사하였다. 수삼의 호흡률은 저장온도가 낮을수록 낮았으나 그 차이는 미미하였다. 수삼 표피층의 정도는 저장기간이 경과됨에 따라 증가하였고, 내부 조직의 경도는 감소하는 경향을 보였으나 저장온도에 따라 경도 변화의 차이는 뚜렷하지 않았다.