Background : Insect pests damages are increasing on the field of Lycium chinense under eco-friendly organic management and especially, pest damage of Gelechiidae (Ilseopsis parki Povolny) is serious. Currently, various eco-friendly organic materials are registered, but insecticidal activity against Gelechiidae was not verified. Therefore, this experiment was carried out to select eco-friendly organic materials showing insecticidal activity in Gelechiidae. Methods and Results : To test insecticidal activity, the Gelechiidae lavas were collected from the field and Nine eco-friendly organic materials were collected from the market. method. The insecticidal activity assay method was, putting Gelechiidae lavas on the 90mm petridish then water-diluted eco-friendly organic materials were sprayed onto the laver. Result of insecticidal activity was observed with a microscope 10 minutes, 2 hours and 18 hours after the treatment. Microscopic observation results, insecticidal activity was shown only in 5 out of 9 materials. Conclusion : As a result, Insecticidal activity against Gelechiidae was not effective in all tested materials, but the 5 eco-friendly organic materials showed the same effect in repeated experiments. In this study, we selected 5 materials out of 9 eco-friendly organic materials against Gelechiidae, then future studies are planned to select the most suitable materials by field testing the 5 eco-friendly organic materials.

Background : Tetraploid plants are bigger in the size of fruits, leaves, stems, and roots than diploid plants due to bigger cells attributed to chromosome multiplication. The advantage of tetraploid plants includes breakdown of self-incompatibility and increase of disease resistance. This study was carried out to gain tetraploid resources for breeding of new boxthorn varieties having pest resistance, higher yield, and self-compatibility. Methods and Results : Tetraploid lines and maternal varieties used in this study were C0148-10 and C0412-1 from Cheongyang-jaerae, M0148-94 and M0148-120 from Myongan, B0148-43 and B0148-78 from Bulro, D0148-62 and D0148-72 from Cheongdae, and Y0148-2 and Y0148-24 from Youngha. For organic acid composition of tetraploid lines and matrenal varieties, malic acid was highest as 1.47 – 4.6 ㎎/g in fruit, and citric acid and succinic acid were highest in leaf as 2.67 – 4.08 ㎎/g and 4.28 – 6.00 ㎎/g. Total organic acid content in root ranged from 1.78 to 3.23 ㎎/g, lower than in fruit and leaf. Of 11 fatty acids composing the boxthorn fruit, linoleic acid was highest as 25.36 – 50.33 ㎎/g. For leaf, linolenic acid was highest as 4.39 – 8.77 ㎎/g. Linoleic acid was highest as 1.65 – 6.98 ㎎/g of all fatty acids in root. 19 free amino acids were analyzed. Average content of essential amino acids in fruit was 6.64% and lysine was highest as 1.57%. Non-essential amino acid content was 8.26% and serine was highest as 2.72% of all non-essential amino acids in fruit. D0148-62 was highest in the total amino acid and the essential amino acid as 23.58% and 10.19%, respectively. Total amino acid content in leaf was 26.49%. Essential amino acid was 12.12% and leucine was highest as 2.08%. Non-essential amino acid was 14.37% and serine was highest as 4.61%. Total amino acid content in root was 13.25%. Essential amino acid was 6.66% and arginine was highest as 2.58%. Non-essential amino acid was 6.59% and serine was highest as 2.60%. Conclusion : Organic acid content increased in fruit of tetraploid lines and lines induced from Cheongyang-jaerae, Myongan, and Cheongdae were higher in contents of linoleic acid, oleic acid and palmitic acid, resulted in total fatty acid increasing. This shows several induced tetraploid boxthorn lines are very useful resources in breeding new varieties.

Background : Tetraploid plants are bigger in the size of fruits, leaves, stems, and roots than diploid plants due to bigger cells attributed to chromosome multiplication. The advantage of tetraploid plants includes breakdown of self-incompatibility and increase of disease resistance. This study was carried out to gain tetraploid resources for breeding of new boxthorn varieties having pest resistance, higher yield, and self-compatibility. Methods and Results : Tetraploid lines and maternal varieties used in this study were C0148-10 and C0412-1 from Cheongyang-jaerae, M0148-94 and M0148-120 from Myongan, B0148-43 and B0148-78 from Bulro, D0148-62 and D0148-72 from Cheongdae, and Y0148-2 and Y0148-24 from Youngha. Betaine content was highest as 0.7 - 1.62% in leaf, followed by 0.55 - 1.17% in fruit and 0.04 - 0.23% in root. Betaine content in plant parts of several tetraploid lines increased compared to martenal varieties, higher in fruit for 5 lines including D0148-72, B0148-78, and C0142-1, higher in leaf for 5 lines including C0148-10, C0412-1, and M0148-94, and higher in root for 7 lines including Y0148-2, M0148-94, and M0148-120. Rutin content in leaf ranged 4.0 – 388.55 ㎎% and was highest as 388.55 ㎎% in Y0148-24. Tannin content in leaf of tetraploid lines was 4.70 - 6.12%, highest as 6.12% in Y0148-2 and M0148-120, similar to the maternal varieties. Youngha of the diploid plants showed the highest tannin content of 7.08%. Total free sugar content in tetraploid lines was higher as 8.53 - 12.53% than maternal varieties. Conclusion : Betaine and rutin contents increased in several tetraploid lines and Total free sugar content in tetraploid lines was higher as 8.53 - 12.53% than maternal varieties. This shows tetraploid boxthorn lines are very useful resources in breeding new varieties.

Background : The most problematic disease in Boxthorn is Anthracnose. New variety with Anthracnose-resistant is good for high yield and Safe fruit production in open field. Therefore it is necessary to develop a New variety with Anthracnose -resistant and high yield. Methods and Results : The new boxthorn line, CB10511-104 was selected from the cross between Cheongun (IT232599) and CB08456-113 to breed new variety with high yield and Anthracnose-resistant in 2011. Its preliminary yield test was performed from 2012 to 2013 and the selected line was named Cheongyang 25. Its regional yield trials were carried out in Cheongyang, Yesan and Jindo for 3 years, from 2014 to 2016. Cheongyang 25 was registered as the new variety, Cheonggeum, in 2016. The specific characteristics were summarized as follows; Tree shape is open type and the leaf is round-lanceolate. The flowers are normal size and purple. The fruit is large size, round oval and yellowish red. The flowering was June 19 with medium flowering. Number of sprout branch by pruning was generated more than the check variety, Cheongmeong. The infection rates on leaves to Eriophys macrodonis Keifer was as strong as 39.6 percent. Anthracnose on fruits in open field was slightly similare compared to the check variety, but check variety was Anthracnose-resistant. The content of betaine and free sugar in dried-fruits were higher than that of the check variety. The dried-fruit yield was decreased about 17 percent in open field. Conclusion : This variety ‘Cheonggeum’ was suitable for cultivation in open field because of Anthracnose-resistant. It is necessary the companion variety, Cheonghong, because of self-incompatible.

Background : Acreage of rain-shelter plastic greenhouse to prevent anthrax is being gradually increased according to growing importance of safe Boxthorn production. But When Boxthorn is grown in the hot season in rain-shelter plastic greenhouse, Fertility decreases. The fertilization rate tends to be different according to cultivated variety and ventilation type of rain-shelter plastic greenhouse. Therefore it is necessary to identify cause and look for a solution. Methods and Results : In the experiment, ‘Cheongun’ cultivar was grown in the rain-shelter plastic greenhouses for the tests. Environment of rain-shelter plastic greenhouses, fertilization rate and density of flower visiting insect were investigated according to ventilation type. Pollen germination and pollen tube elongation on stigma were investigated according to temperature. In mid-July, the temperature on a clear day rose to 45℃ in rain-shelter plastic greenhouse of side vents. Pollen germination and pollen tube elongation on stigma were normal for 3 hours on 30, 35℃. Fertilization rate was somewhat reduced on 40℃ but Pollen germination and pollen tube elongation was good. On 45℃, fertilization rate was very poor and There was no pollen germination. Density of flower visiting insect in rain-shelter plastic greenhouse of roof and side vents was higher than that in rain-shelter plastic greenhouse of side vents. The fertilization rate on high temperature did not show any difference regardless of ventilation type. The rate of artificial cross-fertilization and airborne fertilization were high in rain-shelter plastic greenhouse of roof and side vents. It was necessary to improve airborne fertilization rate because airborne fertilization rate was significantly lower than artificial cross-fertilization regardless of ventilation type. Conclusion : When it was cultivated in rain-shelter plastic greenhouse, the reasons for the lowering of the fertilization rate were as follows. Density of flower visiting insect was low. Boxthorn, which was insect pollination crop, was needed sufficient flower visiting insect on flowering period. But it was insufficiency especially in rain-shelter plastic greenhouse of side vents. Another reason was decrease in pollen germination and pollen tube elongation. Pollen germination was suppressed in the high temperature.

Thise study aimed to determine the optimum antioxidant extraction conditions of dried Gugija (Lycium chinensis Mill). To determine the operational parameters, including ethanol concentration (X1, 0~80%) and extraction time (X2, 1~5 hr), a response surface methodology was applied to monitor brown color intensity, total phenolic compounds, ABTS radical scavenging activity, and Fe2+ chelating activity. Coefficients of determinations (R2) of the models were 0.8486~0.9214 (p<0.05~0.1) in dependent parameters. Brown color intensity of Gugija extracts reached a maximum of 0.75 (OD in 420 nm) under extraction conditions of 2.88 hr in 78.10% ethanol. Total phenolic compounds reached a maximum of 2,355 μg under extraction conditions of 4.94 hr in 30.17% ethanol. ABTS radical scavenging activity was 13.83% at 4.61 hr and 16.21% ethanol. Fe2+ chelating activity showed a maximum of 58.54% under extraction conditions of 3.39 hr in 0.76% ethanol. Optimum extraction conditions (5 hr extraction in 15% ethanol) were obtained by superimposing the contour maps with regards to total phenolic compounds, ABTS radical scavenging activity, and Fe2+ chelating activity of dried Gugija. Maximum values of total phenolic compounds, ABTS radical scavenging activity, and Fe2+ chelating activity under optimum extraction condition were 2,397 μg, 15.62% and 54.78%, respectively.

Background : As the farmers who grow Chinese matrimony vine (Lycium chinense Miller) in rain-shelter greenhouses increase, it is necessary to develop a cultivar with self-compatibility and high quality. Methods and Results : The new boxthorn line, CBP06419-243 was selected from the cross between CBP03310-250(IT232705) and CBP05399-1 to breed the cultivar with self-compatibility and high quality. These lines used for crossing was tetradilpoid induced by the colchicine treatment on bud. Its preliminary yield test was performed from 2009 to 2011 and the selected line was named Cheongyang 18. Its regional yield trials were carried out in Cheongyang, Yesan and Jindo from 2012, 2014, 2015 and Cheongyang 18 was registered as the new variety, Whasu, in 2015. The specific characteristics were summarized as follows; Tree shape is semi-open type and the leaf is lanceolate. The flowers are distinctly larger. The fruit type is medium size, long oblong and yellowish red. The flowering was June 21 with medium flowering. Branching by pruning its sprout was generated less than the check variety, Cheongmeong. The infection rates on leaves to Eriophys macrodonis Keifer was as strong as 7.7 percent. Anthracnose on fruits in open field was slightly severe compared to the check variety, but the infection rates in rain shelter greenhouses was similar. The content of betaine and free sugar in dried-fruits were higher than that of the check. The dried-fruit yield was decreased about 13 percent in open field, but increased 18 percent in rain shelter greenhouses. Conclusion : This variety ‘Whasu’ was suitable for cultivation in plastic film greenhouses because of self-compatibility and high-yielding. It is not necessary the companion variety.

Background : To diversify and increase the demend of Boxthorn, it is breeding for medicinal herbs, food and fresh fruit. Therefore Ningxia with the high sugar content and Black Fruit Containing a lot of functional ingredients have been used for cross breeding. As a result, many new lines were developed. Therefore It was carried out to use as a data for new varieties through the characterization of good lines. Methods and Results : 12 varieties and lines were investigated. Diploid boxthorn were Chengmyeong (Lycium chinense Mill.), Ningxia (Lycium barbarum L.) and Black Fruit (Lycium ruthenicum Murray). Tetraploid boxthorn is Whasu. Breeding Lines were CB13593-8, CB13595-4, CB13592-9, CB13596-1, CB13597-37, CB14643-43, CB14622-2 and CB14626-4. The fruit of lines crossed with Black Fruit (Lycium ruthenicum Murray) were round. The various lines were breeded with black fruit, good fruiting and good soil adaptability. Leaves were small and thin in Compared with tetraploid Boxthorn, Whasu. Sugar content was very high in CB14643-43, CB13595-4, CB13597-37 and CB13593-8. So it’s good for fresh fruit. Conclusion : CB14643-43, CB13595-4, CB13597-37 and CB13593-8 lines were good for fresh fruit because of high sugar content of the fruit. CB14622-2 and CB13596-1 lines were good for food because of fruit color, seed weight and number of seeds. But these are required function component analysis.

Background : Tetraploid plants are bigger in the size of fruits, leaves, stems, and roots than diploid plants due to bigger cells attributed to chromosome multiplication. The advantage of tetraploid plants includes breakdown of self-incompatibility and increase of disease resistance. This study was carried out to gain tetraploid resources for breeding of new boxthorn varieties having pest resistance, higher yield, and self-compatibility. Methods and Results : Tetraploid lines for this study were C0148-10 and C0412-1 from Cheongyang-jaerae (CJ), M0148-94 and M0148-120 from Myongan (MA), B0148-43 and B0148-78 from Bulro (BL), D0148-62 and D0148-72 from Cheongdae (CD), and Y0148-2 and Y0148-24 from Youngha (YH). Flower width ranged 13.2～17.4mm. Flower, pollen and anther were bigger than the mother plants. Pollen germination rate of the tetraploid lines ranging 36.1～58.8% was lower than mother plants (46.5～67.6%). Self-fertilization rate in 4 varieties, MA, BL, CD and YH was low as 1.3%, 4.0%, 1.4% and 4.7% respectively indicating that mother plants are self-incompatible. Self-fertilization rate in tetraploid lines was higher as 58.1～87.5% and B0148-43, M0148-120, and D0148-72 showed the highest self-fertilization rate over 80%. Conclusion : Tetraploid lines showed higer self-fertilization rate than mother plants that they were expected as valuable resources for new boxthorn variety having self-compatibility.

Background : Tetraploid plants are bigger in the size of fruits, leaves, stems, and roots than diploid plants due to bigger cells attributed to chromosome multiplication. The advantage of tetraploid plants includes breakdown of self-incompatibility and increase of disease resistance. This study was carried out to gain tetraploid resources for breeding of new boxthorn varieties having pest resistance, higher yield, and self-compatibility. Methods and Results : 10 Tetraploid lines by colchicine treatment were compared with diploid boxthorn varieties on growth and yield characteristics. Tetraploid lines induced from each varieties were C0148-10 and C0412-1 from Cheongyang-jaerae (CJ), M0148-94 and M0148-120 from Myongan (MA), B0148-43 and B0148-78 from Bulro (BL), D0148-62 and D0148-72 from Cheongdae (CD), and Y0148-2 and Y0148-24 from Youngha (YH). The ratio of stem diameter to stem length in tetraploid lines increased to 0.86 - 1.16 compared to 0.79 - 0.87 of the mother plants whereas number of branches decreased. The ratio of leaf length to width ranged from 2.0 to 3.3, lower than the mother plants. Leaf weight and chlorophyll content also increased compared to the mother plants. C0148-10, C0412-1, Y0148-2, and Y0148-24 showed 34～346% increase in dry fruit yield per 10a compared to mother plants. Conclusion : Above selected tetraploid lines had larger and heavier leaves and C0148-10, C0412-1, Y0148-2, and Y0148-24 were higher in fruit yield than mother plants that the selected tetraploid lines were expected as useful resources for new leaf vegetable and higher yield boxthorn varieties.

Background : ROS produced by oxidative stress damaged endothelial cells, and cause a variety of vascular complications. In diabetic hyperglycemia state, ROS increase. The polyol pathway occur in diabetic complications, the excess glucose is absorbed into the polyol pathway when aldose reductase increased, NADPH changes it to sorbitol. Glutathione (GSH) removes ROS. GSH level is reduced by glutathione reductase, using NADPH as an electron donor. Activation of the polyol pathway decrease NADPH, and GSH also reduced. As a result, ROS is increased. In diabetic hyperglycemia state, Glycolysis increases. Effects of increased glycolysis, protein kinase C (PKC) is increased. NAD(P)H oxidase, stimulated by PKC-dependent pathway, increases ROS in the cell. In this study, we measured the ROS scavenging activity of 5 natural products (Lycii fructus, Astragalus membranaceus, Cassia Tora, Polygonatum odoratum, Rubus Coreanus), to confirm the efficacy as diabetic antioxidants. Methods and Results : We extracted 5 natural product by distilled water and ethanol. DPPH radical scavenging activity was significantly higher in Lycii fructus, Rubus coreanus. ABTS radical scavenging activity was better Rubus coreanus, Lycii fructus, Cassia Tora. In addition to, Rubus coreanus, Cassia Tora, Lycii fructus was comparatively higher reducing power activity than other natural products. And total phenolic and flavonoid contents were much higher in Rubus coreanus compared with other extracts. Conclusion : These results suggest that Lycii fructus, Rubus coreanus can be applied as diabetic antioxidant that prevent vascular complications caused by ROS.

As the farmers who grow Chinese matrimony vine (Lycium chinense Miller) in rain-shelter greenhouses increase, it is necessary to develop a cultivar with early-flowering. In addition, the new varieties should be continuously selected in order to adapt to climate change and to respond to future demands. The new line, CB07423-104, was selected from the cross between Cheongmyeong (IT232706) and CB04341-286 in 2007 to breed a cultivar with early flowering and high yielding. Its preliminary yield tests were performed from 2009 to 2010 and the selected line was named Cheongyang 17. Its regional yield trial was carried out in Cheongyang, Yesan and Jindo from 2011 to 2013 and Cheongyang 17 was registered as the new variety, ‘Cheonghong’ (Certificate on grant of plant variety rights: Grant number No.5642, Protection period 06/07/2015 ~ 05/07/2035) in 2013. The major characteristics were summarized as follows; The plant shape was spreading type. Leaf had ellipsoidal small sizes and 3~5 leaves on a node. The fruit was oblong and yellowish red. The flowering time was June 17 and faster about 11 days than the check ‘Cheongmyeong’. The branching was generated less than the check variety. This cultivar has a lot of flowers bloom per a node and many fruits per a plant. The resistances to anthracnose and eriophyidae mite were a little better than that of the check variety. The content of betaine of dried-fruit and brix degree of fresh fruit were higher than those of the check. The dried-fruit yield was 16 percent higher than the check at regional yield trials conducted at Cheongyang, Yesan and Jindo. Applications are customarily available in both medicinal and edible. This variety has self-incompatibility. So, the companion variety, Cheongmyeong, was required as pollinizer and recommended to plant 1 : 1.

Background : Tetraploid plant is bigger in size of organs like leaf, stem, fruit, and root than diploid plant by chromosome doubling. Also, Self-ìncompatibílity disappeares and disease resistance is strengthened in tetraploid plant. To breed new boxthorn variety having bigger fruit, higher yield, pest tolerance, and, self-compatibílity, tetraploids was induced for tetraploid breeding resources by colchicine treatment in each boxthorn variety. Materials and Methods : Colchicine was treated in Myongan, Cheongdae, Bulro, and Youngha for 0.1% concentration and 48 hours. Polyploid was identified by flow cytomertry(Partec, PA-1) and chromosome numbers of polyploid individuals were examined by aceto-carmine stain. Conclusion : Colchicine was treated in Myongan, Cheongdae, Bulro, and Youngha for 0.1% concentration and 48 hours. Tetraploid was induced as 13, 9, 6, and 5 individuals in Myongan, Cheongdae, Bulro, and Youngha respectively by above colchicine treatment.

Background : Recently new breeding goal setting is required to develop new boxthorn variety with superior treats of bigger fruits, higher yield in fruits and sprout, pest tolerance, higher medicinal functionality, and self-compatiblity. Various genetic resources also should be retained for this goal. This study was carried out to obtain boxthorn tetraploid for breeding resource. Methods and Results : Colchicine concentration and treatment time for polyploid induction were 0.1, 0.2, 0.4, 0.6% and 12, 24, 36, 48hours respectively. Polyploid was identified by chromosome numbering of individuals by aceto-carmine stain which were examined for polyploid by flow cytomertry(Partec, PA-1) in advance. Conclusion : When cuttings of Cheongyang-jaerae were treated by colchicine to induce tetraploid, survival rate decreased as colchicine concentration and treatment time increased. Rate of individuals having over 30 chloroplast number of stomata guard cell was highest as 37.5% in colchine 0.1% and 48 hours treatment. 8 of them were identified as polyploid by flow cytometry and tetraploid induction efficiency was highest as 6.0% in colchine 0.1% and 48 hours treatment. According to chromosome number examination, 3 of 8 individuals were aneuploid and 5 were tetraploid.

Background : Acreage of rain-shelter plastic greenhouse to prevent anthrax is being gradually increased according to growing importance of safe Boxthorn production. But When Boxthorn is grown in the hot season in rain-shelter plastic greenhouse, quantity of Boxthorn decreases. therefore the research was carried out to investigate Fertilization mode. Methods and Results : Chungwoon was very strong self-incompatibility. Chengyangjaerae, Chengyang18 and CBP11542-206 was self-compatibility. Artificial fertilization rate was slightly higher in roof and side vents than in side vents of rain-shelter plastic greenhouse in hot season. Pollen sterility due to high temperature is not critical because artificial fertilization rate was high in side vents of rain-shelter plastic greenhouse in hot season. Airborne fertilization rate was significantly lower in all varieties. Open fertilization rate was higher in roof and side vents than in side vents of rain-shelter plastic greenhouse Conclusion : Roof and side vents was good in compared to side vents in rain-shelter plastic greenhouse of Boxthorn because of high Airborne and open fertilization rate. Self-compatibility varieties were better than self-incompatibility varieties in rain- shelter plastic greenhouse because of high open fertilization rate.

Background : Fruit picking is the most labor consuming procedure for Boxthorn (Lycium chinense Mill.) growers. Recently fruit harvesters were invented and improved in the efficiency to fulfill the expectation of farmers through field demonstration tests. Additionally, T-type hedge cultivation method harmonized well with fruit picking machine, but it also needed to be improved in its’ pruning methods for the efficiency of fruit collecting procedure. Methods and Results : In this experiment, ‘Cheong-un’ cultivar and local variety were grown in the rain shelter greenhouses for the tests. Plots were set by randomized block design. There were two types of previously developed harvesters. One vibrates it’s harvest rod right and left (A type) and the other shakes it’s rod back and forth (B type). Among two harvesters A type was superior to B type in efficiency and convenience in use and it was able to harvest eleven times more fruits than manual picking in simple comparison test. In field comparison test, A type harvester performed 4.6 to 5 times more fruits than manual fruit picking. T-type hedge cultivation method is the proper way of enhancing efficiency of harvester. In the test, wiry perpendicular branches formed by once pruning method hindered moving forward of fruit collector. However installing collecting net with velcro along the edge under the field increased the efficiency and handiness of fruit harvester and well harmonized with T-type hedge cultivation. Conclusion : From the above results, the newly developed fruit harvester could increase the annual income of Boxthorn growers by reducing fruit picking labor and time.