This research was conducted to determine the effect of condensed tannin (CT) on the quality of fermentation and nutritive value of "Demark" alfalfa. During the ensiling, the production of NH₃-N and NDF decreased while the concentration of lactic acid in increased apparently as the CT was added.

Two-year-old, full flower aohan alfalfa (Medicaga sativa cv. aohan) was chosen to evaluate the silage effect by using different additives of Qingbao Ⅱ , Caihe and Yishengkang. Result indicated that additives increased CP content (P＜0.05) and decreased CF, ADF and NDF content significantly (P＜0.05). Lactic acid content in additives treatments were significantly higher (P＜0.01) than control. These result showed that additives improved silage fermentation quality, reduced cellulose contents and improved silage nutritive value. Among three treatments, Caihe and Yishengkang were priority to Qingbao Ⅱ on the effect of promoting the desired lactic acid fermentation.

39 accessions of sickle alfalfa (Medicago falcata L.) collected from different regions of China were evaluated for their tolerance to drought. Differences were observed among accessions in their reactions to drought and 8 good tolerant accessions at populations level and 18 excellent tolerant accessions at individual level were identified based on drought response index (DRI).

Biolog ECO plates (31 different carbon sources, Biolog Inc., Hayward, USA) were used to discriminate between rhizosphere soil samples from 4- and 9-year alfalfa stands each with three replication. The growth curves for different groups of carbon sources were nearly sigmoidal, but the maximum rate of utilization was faster for amino acids, carbohydrates and polymers t㏊n for amides, miscellaneous and carboxylic acids, and carbon sources utilization efficiency were all higher in 9-year t㏊n in 4-year alfalfa stand.

Research on alfalfa root morphology and health is very important in development of technology for efficient improvement and production of alfalfa. The objectives of this study were to evaluate the root morphology and health of two alfalfa varieties, Algonquin and Golden Queen, to determine relationships among root morphology traits and root health. Results from these analyses indicated t㏊t yield was 6.77-43.93 t/h㎡, total root length ranged from 215.17-708.89 ㎜, root surface area ranged from 93.61-717.06 ㎠, volume ranged from 3.24-57.72㎤. Results from these analyses indicated t㏊t root infestation score was the lowest averaged over age of alfalfa stand in Algonquin. Yield in 2-yr old stands was greater in Golden Queen compared to Algonquin.

Seedling drought resistance of three alfalfa cultivars-Medicago sativa L. cv. Aohan, Medicago sativa L. cv. Zhaodong and Medicago sativa L. cv. Tumu No.2 were studied and evaluated. The permeability of plasma membrane, the free proline content and the peroxidase (POD) activity of three alfalfa cultivars were tested at 24h after treating with 20% polyethylene glycol (PEG-6000). The results indicated t㏊t the drought resistance ability of all these varieties was in the order of Medicago sativa L. cv. Aohan, Medicago sativa L. cv. Zhaodong and Medicago sativa L. cv. Tumu No.2.

Laboratory tests were conducted to determine the susceptibility of the alfalfa weevil, Hypera postica to Korean entomopathogenic nematodes, Steinernema carpocapsae GSN1 strain (ScG), S. glaseri Dongrae strain (SgD), Heterorhabditis bacteriophora Hamyang strain (HbH), and Heterorhabditis sp. Gyeongsan strain (HeG) at the petri dish assay. The larval mortality of H. postica was significantly different depending on nematode species and treatment concentration. SgD and HeG strain were more effective against H. postica larva than ScG and HbH strain. When SgD and HeG strain were treated with the rate of ＞20 infective juveniles (ijs) per alfalfa weevil larva, mortality was ca. 77.5-100% at the late instars of H. postica in 3 days. The number of established nematode was significantly different depending on nematode species, whereas number of progeny was not significantly different. The mean number of established ijs of SgD strain in a host was the highest at 80 ijs by 30.2. The highest progeny number of HeG strain was 2,671.5 with 80 ijs. All nematode strains were not parasitic entirely to H. postica adults. These results show that alfalfa weevil late larva is highly susceptible to Korean entomopathogenic nematodes in the laboratory.

알팔파(Medicago sativa L.)의 치사온도를 결정하기 위하여, "Vernal" 품종을 시험재료로 하여 종자를 Petri dish에서 발아시켜 작은 화분에 10 개체씩 이식, 생장실에서 4주간 재배하였다. 45, 50 및 에서 처리한 경우에는 60분간 처리했을 때에도 거의 식물체 손상이 없었다. 6에서 60분간 처리했을 때에는 잎이 약간 시든 듯한 현상이 있었으나 식물체의 손상은 거의 없는 상태였으며, 에서 60분간 처리했을 때에도 에서 처리한

알팔파 종자를 의 2,4-D를 첨가한 SH 배지에서 배양하여 캘러스를 유도하였으며, 캘러스를 SH 배지에 의 NAA와 의 kinetin을 첨가한 배지에서 28~30일간 배양하고, 의 2,4-D와 의 kinetin을 첨가한 배지에서 3~5일간 배양 후, 의 (와 의 proline을 첨가한 배지에서 21~25일간 배양하여 완전히 재분화된 식물체와 많은 수의 shoot를 얻었다. 알팔파의 재분화 과정에서 뿌리가 유도되는 효율을 높이기 위해, SH salt의

본 시험은 알팔파 Vernal(대조품종), Anchor, P5444, DK120, DK125, Horizon, DK135, 루나및 기타와카바 9품종을 축산기술연구소 대관령지소(표고 800m)와 남원지소(표고 450m)에서각각 난괴법 3반복으로 수행하였다. 알팔파의 엽폭은 Vernal을 제외하고 모든 품종이 대형이었으며 개화기는 DK125가 가장 빨랐다. 초고는 Luna품종이 가장 컸으며 내한성은 P5444가 80.2%로 가장 높았다. 알팔파의 건물수량은

질산태 질소의 공급수준이 알팔파의 영양생장기 동안의 질소동화와 성장 및 재생기간 동안의 뿌리조직내 전분과 질소 축적에 미치는 영향을 규명하고자 0.2, 1.0 및 3.0 mM KN 하에서 10주 동안의 영양생장 후 건물 및 질소 화합물의 식물조직내의 분포를 분석하고, 24일간 1차 및 2차 재생을 각각 시킨 후 뿌리조직에 축적된 전분과 질소 함량을 분석하였다. 10간의 영양생장 후 건물과 질소화합물의 함량을 1.0 mM 처리구를 기준으로 비교한 결과,

본 시험은 알팔파 건초의 혼합비율을 60%(A), 50%(B), 40%(C) 및 30%(D)의 4가지로 달리하여 조제한 시험사료를 이유 후 한국 재래산양에게 급여하여 사료 섭취량과 소화율 및 증체에 미치는 영향을 조사하여 이유 후 초기 사료 개발을 위한 기초자료로서 활용하고자 본시험을 실시하였으며, 그 결과를 요약하면 다음과 같다. 시험사료의 화학적 조성분은 조단백질 함량을 모두 18%로 고정시켜 동일하였으며 ADF, NDF 및 조회분의 함량은 A구가

내열성 유전자인 BcHSP17.6를 갖도록 제작한 발현벡터 pBKH4를 Agrobacterium tumefaciens LBA 4404에 도입후, Agrobacterium과 알팔파 캘러스의 공배양을 통해 감염시킨 캘러스를 의 kanamycin과 의 cefotaxim을 첨가한 SH-kc배지에서 배양하며 형질전환된 캘러스를 선발하였다. 식물체 재분화는 SH- nk-c, SH-sp-c, SH-11b-c, SH-1BA 배지에서 약 4개월간 배양하여 재분화를 완성