The intermediate shaft of sliding type is assembled with coated shaft joint and tube joint. Since the intermediate shaft plays a role of absorbing displacement change due to vibration, the intermediate shaft must have a sliding force value in an appropriate range. In this study, an intermediate shaft assembly system for post-processing of defective intermediate shafts was developed. The intermediate shaft assembly system consists of a wear count prediction model and an automatic wear system. A wear count prediction model was created with the initial assembly sliding force, quality, and set values. As a result of applying the intermediate shaft assembly device, the sliding force of the intermediate shaft was induced within the set value range. And it was prevented from the intermediate shaft defect and eliminated manual work.
In emergency situations such as nuclear accidents or terrorism, radioactive and nuclear materials can be released by some environmental reasons such as the atmosphere and underground water. To secure the safety of human beings and to respond appropriately emergency situation, it is required to designate high and low dose rate regions in the early stages by analyzing the location and radioactivity of sources through environmental radiation measurement. This research team has developed a small gamma probe which is featured by its geometrical accessibility and higher radiation sensitivity than other drone detectors. A plastic scintillator and Silicon Photomultiplier (SiPM) were applied to the probe to optimize the wireless measurement condition. SiPM has a higher gain (higher than 106) and lower operating voltage (less than 30 V) compared to a general photodiode. However, the electronic components in the SiPM are sensitively affected by temperature, which causes the performance degradation of the SiPM. As the SiPM temperature increases, the breakdown voltage (VBD) of the SiPM also increases, so the gain must be maintained by applying the appropriate VBD. Therefore, when the SiPM temperature increases while the VBD is fixed, the gain decreases. Thus, the signal does not exceed the threshold voltage (VTH) and the overall count is reduced. In general, the optimal gain is maintained by cooling the SiPM or through a temperature compensation circuit. However, in the developed system, the hardware correction method such as cooling or temperature compensation circuit cannot be applied. In this study, it was confirmed that the count decreased by up to 20% according to the increase in the temperature of the SiPM when the probe was operated at room temperature (26°C). We propose methods to calibrate the total count without cooling device or compensation circuit. After operating the probe at room temperature, the first measured count is set as the reference value, and the correction factor is derived using the tendency of the count to decrease as the temperature increases. In addition, since this probe is used for environmental radiation monitoring, periodic measurements are more suitable than continuous measurements. Therefore, the temperature of the probe can be maintained by adding a power saving interval to the operation sequence of the probe. These two methods use the operation sequence and measurement data, respectively. Thus, it is expected to be the most effective method for the current system where the temperature compensation through hardware is not possible.
Radiological characterization is important in decommissioning and dismantling of nuclear facilities, in order to assess the radioactivity concentration, classify the wastes, and secure workers’ safety. The Some components such as Reactor Pressure Vessel (RPV) in nuclear facilities has dose rate higher than Sv/hr, thus in-situ gamma spectroscopy systems suffer from a very high count rate which causes energy resolution degradation, photo-peak shift, and count loss by pile-up and dead-time. The system must be operated in a very high count rate, in order to measure spectra precisely and to quantify radionuclide contents. In order to apply in-situ measurement in high radiation dose rate environment, the sensor, front-end electronics, and data acquisition (DAQ) should be carefully selected and designed as well as precise design of collimators and radiation shield. In this paper, the components of the detector system were selected and performance was evaluated in a high count rate before design the collimator and shield. A LaBr3 coupled with a PMT having short decay time constant (16 nsec) was selected for high count rate application, and two different amplifiers (a conventional charge sensitive preamplifier with 50 usec decay time constant, and wide-band voltage amplifier) were tested. As DAQs, DT5781 (14 bit, 100 MS/s, CAEN) of Pulse Height Analysis (PHA) which is conventionally used signal processing method in the gamma spectroscopy, and DT5730 (14 bit, 500MS/s, CAEN) of Pulse Shape Discrimination (PSD) which is similar to Charge to Digital Convertor (QDC) were used. The number of photons incident to the detector was varied by changing the detector-source distance with Certificate Radiation Material (CRM), and compared to the output count rate. The count rate capability, and energy resolution with different amplifier and DAQ was evaluated. Additionally, the performance of DAQs in extremely high count rate was evaluated with signal data generated by the emulator which can simulate the detector signal waveforms fed into the DAQ based on the measured spectrum.
During her time as Emperor of the Tang Dynasty, Wu Ze Tian created a number of new characters. Later generations called them “Wu Queen New Characters”, and some also called “Ze Tian Characters” and “Ze Tian Xin Characters.” Since the Tang Dynasty, although many scholars have studied the writing methods, word counts, and circulation areas of new characters, there are many different opinions, especially about the number of new characters and that of glyphs, which makes future studies much more difficult and impossible to restore the original appearance at that time. In addition, the timing of the promulgation of each new word is hardly recorded in the classics, and it is difficult to know the actual use of the word and the circulation situation. Therefore, this article uses Collection of Epitaph of Tang Dynasty, Sequel Collection of the Epitaph of Tang Dynasty, Collection of Epitaph of Tang Dynasty and Research, and Beijing Library Collection of Stone Carving of Each Chinese Generation etc. to set the age of the research material from “Zai Chu” first year (A.D.689) to the end of “Chang An” fourth year (A.D.704), 16 years in total, 422 piece of epitaphs. They are arranged according to the grave owner’s burial time, sorting out Queen Wu’s implementation process for new words, actual use of new words, word count, and word font, and staging and spread area. By means of this, the article makes the appearance of Queen Wu’s new words possible, even further presented in front of everyone, and finally making a new investigation for the Queen Wu’s new words.
The purpose of this study was to analyze the effects of the pre-treatment method on the measurement of probiotic cell counts. The probiotic cell count was not significantly different in the pre-treatment method such as experimenters, diluted solution, medium, and homogenization duration. The mean value of probiotic cell count with capsule was 2.2×1010±9.5×109 CFU/g. This probiotic cell count was converted into 2.8×1010±1.2×1010 CFU/g based on the net weight. The mean value of probiotic cell count without capsules was 4.3×1010±1.8×1010 CFU/g. As a result of this comparison, probiotic cell count showed significant difference with and without capsules. Thus, it is suggested that the probiotic cell count is measured by removing the capsule in capsule probiotics.
To enumerate Staphylococcus aureus in food, Baird-Parker Agar (BPA) is usually used in the conventional method, However it requires time and space for the preparation and plating, and incubation. Thus, use of the 3MTM PetrifilmTM Staph Express Count Plate (STX Petrifilm) might be appropriate to solve these challenging problems. The purpose of this study was to compare the efficiency of STX Petrifilm with BPA for enumeration of S. aureus in various foods. A mixture of S. aureus strains ATCC29213, ATCC25923, and ATCC13565 was inoculated on marinated pork chop, beef (chuck tender), dried filefish, semi-dried squid, rice cake, and Japchae (stir-fried glass noodles) at 2, 3, 5, and 7 Log CFU/g. S. aureus cell counts were enumerated by spread-plating on STX Petrifilm and BPA after 0 and 24 hours at 4oC (marinated pork chop, beef, semi-dried squid, and stir-fried glass noodles) and 25oC (dried filefish and rice cake). Recovery of STX Petrifilm for S. aureus from various food samples was compared with BPA, and the results showed that there were no significant differences between two selective media in all cases. The results indicated that STX Petrifilm had enough efficiency to recover S. aureus from various foods as well as saving time and space.
V. vulnificus는 그람음성의 호염성균으로 감염 되었을 경우, 복통과 발열 등의 급성 위장염을 일으키며 만성질환자에게 급성 패혈증을 일으키는 매우 높은 치사율의 식중 독균이다. 식품 중에서 V. vulnificus를 분석하는 방법으로 는 TCBS agar와 같은 선택배지를 이용하는 방법과 PCR 을 이용한 방법이 있으나 온도, 염 및 pH 등과 같은 환경요인에 민감한 V. vulnificus의 특성을 고려하였을 때 정확한 균수 정량을 위한 정량분석법 확립의 필요성이 요구된 다. 본 연구에서는 배지 및 염 차이에 따른 V. vulnificus 생육 특성 차이에 대한 연구를 진행하였다. 그 결과, V. vulnificus 균수 정량분석에 APW 증균 배양을 이용한 MPNPCR 방법이 적합하였다. 본 연구에서 제시된 방법은 해수뿐만 아니라 어패류 등의 시료에서 V. vulnificus 균수 정 량분석에 유용하게 사용될 것으로 기대된다.
Based on the collaborative and social characteristics, social networking services (SNSs) are growing platforms for consumer-to-consumer conversation including electronic word-of-mouth (eWOM). eWOM is defined as ‘any positive or negative statement made by potential, actual, or former customers about a product or company, which is made available to a multitude of people and institutions via the Internet’ (Hennig-Thurau, Gwinner, Walsh, & Gremler, 2004, p.39). Despite its academic and managerial significance, several preliminary studies have examined what determines effective eWOM in SNSs (e.g., Chu & Kim, 2011; Teng, Khong, Goh, & Chong, 2014). Extending these attempts, we empirically investigate what influences consumers’ evaluation of eWOM, especially on Facebook. We suggest a new perspective of focusing on the psychological stream of social identity (Tajel & Turner, 1986) which is a distinctive factor explicit on Facebook. Unlike conventional eWOM platforms like bulletin boards and online communities where the user information is limited due to anonymity or presented in restricted manner (e.g., reputation from ratings by other members) (Dou, Walden, Lee, & Lee, 2012), Facebook provides profile information about users including one’s social group membership (e.g., school, company). This implies that the perception of social identity on Facebook may influence how one evaluates the source and his/her eWOM message.
PURPOSES: The demand for extending national highways is increasing, but traffic monitoring is hindered because of resource limitations. Hence, this study classified highway segments into 5 types to improve the efficiency of short-term traffic count planning. METHODS : The traffic volume trends of 880 highway segments were classified through R-squared and linear regression analyses; the steadiness of traffic volume trends was evaluated through coefficient of variance (COV), and the normality of the data were determined through the Shapiro-Wilk W-test. RESULTS : Of the 880 segments, 574 segments had relatively low COV and were classified as type 1 segments, and 123 and 64 segments with increasing and decreasing traffic volume trends were classified as type 2 and type 3 segments, respectively; 80 segments that failed the normality test were classified as type 4, and the remaining 39 were classified as type 5 segments. CONCLUSIONS : A theoretical basis for biennial count planning was established. Biennial count is recommended for types 1~4 because their mean absolute percentage errors (MAPEs) are approximately 10%. For type 5 (MAPE =19.26%), the conventional annual count can be continued. The results of this analysis can reduce the traffic monitoring budget.
PURPOSES: This study has been conducted to determine a homogeneous segment and integration to improve the efficiency of short-term traffic count. We have also attempted to reduce the traffic monitoring budget.
METHODS: Based on the statistical approach, a homogeneous segment in the same road section is determined. Statistical analysis using t-test, mean difference, and correlation coefficient are carried out for 10-year-long (2004-2013) short-term count traffic data and the MAPE of fresh data (2014) are evaluated. The correlation coefficient represents a trend in traffic count, while the mean difference and t-score represent an average traffic count.
RESULTS : The statistical analysis suggests that the number of target segments varies with the criteria. The correlation coefficient of more than 30% of the adjacent segment is higher than 0.8. A mean difference of 36.2% and t-score of 19.5% for adjacent segments are below 20% and 2.8, respectively. According to the effectiveness analysis, the integration criteria of the mean difference have a higher effect as compared to the t-score criteria. Thus, the mean difference represents a traffic volume similarity.
CONCLUSIONS : The integration of 47 road segments from 882 adjacent road segments indicate 8.87% of MAPE, which is within an acceptable range. It can reduce the traffic monitoring budget and increase the count to improve an accuracy of traffic volume estimation.
국내 상수원을 대상으로 시행하고 있는 조류경보제는 남조류 발생 현황을 취 정수장 등 물관리 기관에 전파하여 대응조치를 유도하는 제도로 신속하고 정확한 남조류 계수를 필요로 한다. 따라서 조류경보제 발령 기준 대상 남조류인 Anabaena, Aphanizomenon, Microcystis, Oscillatoria 속의 군체 크기와 세포수의 상관관계를 조사하고 회귀식을 도출하여 군체 크기로 세포수를 계산할 수 있는 방법을 알아보고자 하였다. 2013년 8월부터 10월까지 남조류가 과다증식한 시기에 한강(팔당호), 낙동강(달성보, 창녕함안보) 및 금강(고복저수지)의 대표지점에서 남조류 시료를 채집하였으며, 조류경보제 발령 기준 대상 남조류 속의 종별 군체 크기와 세포수의 상관 관계를 조사하여 종 및 속별 회귀식을 산정하였다. 남조류의 속별 상관분석 결과는 사상형인 Anabaena와 Aphanizomenon의 r2값이 0.93 이상으로 높은 상관성을 보였으며 구형의 Microcystis는 0.76의 상관계수 값을 나타냈다. 종 별 상관분석 결과 사상형 남조류 Anabaena crassa, Aphanizomenon flos-aquae, A. issatschenkoi, Oscillatoria curviceps, O. mougeotii는 r2값이 0.89~0.96의 범위로 높은 상관성을 나타냈으며, 구형인 Microcystis aeruginosa, M. wessenbergii, M. viridis는 0.76~0.88의 상관계수 값을 나타냈다. 다른 속에 비해 상대적으로 Microcystis의 상관성이 낮게 나타난 이유는 동일한 종, 동일한 크기의 군체라도 Microcystis strain에 따라 점액질 내의 세포 밀집 정도와 세포 크기에 차이가 있기 때문이다. 본 연구 결과 도출한 회귀식을 이용하여 군체 크기 측정값을 세포수로 환산하는 방법이 기존의 세포 계수법과 비교할 때 신속하고 간편할 것으로 보인다. 향후 남조류 종별 더 정확한 회귀식을 도출하기 위해서는 많은 시료수 확보와 더불어 다른 종들에 대한 조사 연구가 진행되어야 할 것이다.
본 연구는 미생물 첨가가 느타리버섯 폐배지의 영양소 함량, 발효특성 및 미생물상에 미치는 영향을 조사하기 위해 수행하였다. 시험은 느타리버섯 폐배지에 미생물 무첨가구(CON), 고초균 첨가구 (Bacillus subtilis 2⨯1010 cfu/g; BS), 유산균 첨가구(Lactobacillus plantarum 2⨯1010 cfu/g; LP) 및 혼합균 첨가구(Bacillus subtilis : Lactobacillus plantarum = 1:1; BL)로 4 시험구를 설정하고 6일간 발효시켰다. 발효 직후 0, 1, 2, 4 및 6일에 시료를 채취하여 분석에 이용하였다. 발효 6일후 조단백질과 조지방 함량은 BS구에서 가장 높았다(P<0.05). NDF 함량은 LP구와 BL구에서, ADF 함량은 미생물을 첨가한 모든 시험구에서 높은(P<0.05) 반면, in vitro 건물소화율은 CON구가 높았다 (P<0.05). Lactate와 propionate 함량은 CON구와 BS구가 타 시험구에 비해 높았다(P<0.05). 한편 발효 0일에 유산균 함량은 BS구와 LP구에서 높았으며(P<0.05), 발효 1일에 유산균 함량은 LP구에서, 고초균 함량은 BL구에서 각각 높았다(P<0.05). 발효 2일에 lactate와 propionate 함량은 LP구와 BL구에서 타 시험구에 비해 높았다(P<0.05). 이상에서와 같이 BS 첨가는 발효과정 중 유기산 생성량 개선에는 유리하나 건물소화율에는 부정적인 영향을 미칠 것으로 사료된다.
표준 평판배지 정량검출법과 TEMPO STA를 이용한 황색포도상구균 정량 분석에 대한 상관성과 유의성을 평가 하기 위해 복합조리식품에 황색포도상구균을 인위적으로 접종하여 결과값을 통계 처리하여 유의성을 조사한 결과 두 실험방법간에는 유의적 차이가 없었다(p < 0.05). 상관 계수(r2)는 0.9672로 두 실험간에는 높은 연관성을 보였다. TEMPO STA를 466개의 시료에 적용한 결과 454개 (97.4%)의 시료는 문제가 없었으나 이중 12개의 시료에서는 error가 발생하였다. 그러나 TEMPO법은 실험 단계 간편하고 사용이 쉬우며 신속한 결과를 얻을 수 있으며 자동화된 장비로 실험 과정에 의한 오류를 최소화 할 수 있는 장점이 있으며 표준시험법인 표준 평판 배지법과도 유의적인 차이가 없어 정확한 정량검출이 가능할 것으로 생각된다. 최근 식품의 안전성확보를 위해 검사 품목이 다양해지며 수도 증가하는 추세이기 때문에 황색포도상구균의 정량 검사가 정확하고 신속해야 할 필요가 있으므로 TEMPO STA법의 필요성은 앞으로 크게 늘어날 것으로 보인다.
농산물의 안전성 확보와 품질평가를 위해서 신속하고 경제적인 비파괴 검사법에 대한 연구 및 기술개발이 활발하게 이루어지고 있다. 그러나 분광된 빛을 이용하여 내부 품질평가 과정에서 발생할 수 있는 분석 대상체의 성분 변화에 미치는 영향에 대한 연구는 미비한 실정이다. 따라서 본 연구는 분광분석을 이용하여 원유의 성분분석 과정에서 분광된 빛이 원유의 성분 및 체세포에 미치는 영향을 구명하고자 수행되었다. 본 연구에 사용된 원유는 일본 시가현 소재 낙농가에서 채취된 것으로 유지방, 유단백질, 유당, 무지고형분, 총고형분, 유요소, 구연산 및 체세포수를 화학적 방법에 의해 측정하였다. 또한 인위적으로 분광된 빛은 5가지 영역대로 구분하여 원유에 각각 1분, 5분, 10분간 주사하였다. 연구결과, 400 nm이하 파장대인 자외선 영역에서 유지방이 2.6% 증가되는 경향이 있었고, 체세포수도 9.0% 증가된 것으로 분석되었다. 그러나 다른 원유 성분에는 변화가 없는 것으로 나타났다. 따라서 자외선 영역의 빛을 원유 품질 평가에 적용하기 위해서는 보다 다양한 원유 시료에 대한 추가 검증이 필요할 것으로 사료된다.
고려전기에서 11세기의 한문학은 왕실을 중심으로 이루어진 貴族文學이며, 遊興的文學이라고 할 수 있다. 왕실을 중심으로 한 이 시기의 문단은 형식과 내용에서 개성을 잃은 채 彫蟲篆刻하다는 비판을 받기에 이른다. 주로 유교적 성향의 문인들이 비판적 입장을 표명했는데 그들은 그 폐단을 극복하기 위해 노력을 경주하게 된다. 그들은 文에서 浮華無實한 문장을 극복하기 위해 화려한 형식미를 추구하지 않으며, 백성을 교화시키고 좋은 풍속을 이루는 내용을 지닌 古文에 관심을 갖게 된다. 또한 내용을 중시하는 입장을 표명한 이들은 시에 現實批判과 지배자에 대한 諷諫과 敎訓을 담고 있는 시를 창작하게 된다. 다른 입장을 지니고 있는 이들은 시를 어떻게 형상화하느냐에 관심을 기울였는데, 용사를 중시하는 경향을 띠기도 하고 핍진한 경물묘사에 치우치는 모습을 보이기도 하면서 조충전각한 문풍을 극복하려고 노력하였다. 이러한 노력들은 그 당시에 새로운 기풍을 불러 일으켰으며 다음시기에 영향을 미치게 된다.