Citrus is one of the major fruits produced in Korea. There are about 20 species mainly grown in Jeju Island, Korea. Four representative species, which are quite different in the shape of leaf and the taste of fruit, were selected and were used to profile the transcriptomes. These species are ‘Miyagawa Wase’ (C. unshiu Marcov.) satsuma mandarin, ‘Kiyomi’ (C. unshiu Marcov. × C. sinensis) mandarin hybrid, ‘Dangyuja’ (C. grandis) and ‘Natsudaidai’ (C. natsudaidai). Classification of the up-regulated and down-regulated genes using the Cluster of Orthologous Groups of proteins (COG) database reveals that the number of genes included in each group differed significantly among the four species. Several genes that showed significant differences in expression on the microarray were selected and their expression patterns were examined by reverse transcription- ploymerase chain reaction. Metabolic genes such as tyrosine decarboxylase and β-glucosidase ligase were found to be highly expressed in Miyagawa Wase, relative to other species. On the other hand, the expression level of mannose phosphate isomerase was lower in Miyagawa Wase. An efflux pump gene was found to be up-regulated in Kiyomi, whereas cinnamyl-alcohol dehydrogenase was down-regulated. β-carotene 15,15’-dioxygenase, which is involved in the vitamin metabolism, was up-regulated in Natsudaidai. Interspecific differentiations of gene expression are analyzed in terms of the metabolic pathways and their possible roles in citrus species.

본 연구에서는 당유자 원료의 세절방법과 당유자 정백당 혼합물의 냉동저장 기간 및 당유자청의 희석배수에 따른 당유자차의 관능적 평가를 실시하고 전반적인 기호도에 대해 조사하였다. 원료선별, 세척, 건조, 절단 및 씨앗제거 과정을 거친 당유자를 슬라이스 세절 후 당침하거나 믹서기로 갈고 과육과 과피를 혼합 당침한, 두 종류의 당유자청을 제조하고 이를 적정 비율로 희석하여 제조한 당유자차의 맛, 색상과 향을 비교하였다 또한 원료세절 후 정백 당에 당침