In vitro development of parthenogenetic embryo was examined after ethanol treatment of follicular oocytes matured in vitro for 42, 48, 54 and 60h in the pig. The follicular oocytes were matured in TCM 199 containing 15% FCS and gonadotrophins in an atmosphere of 39 5% . The cumulus-free oocytes were activated by 10% ethanol treatment in M2+4mg /ml BSA for 10 min. The ethanol-activated oocytes were washed and further cultured in TCM199+20%FCS containing granulosa cell monolayer. Maturation rates at 42, 48, 54 and 60h of IVM were 75.0, 86.5, 81.6 and 87.9%, respectively. Thus the oocytes maturated in vitro for longer periods did not improve nuclear maturation much. Pronuclear formation rates at 18h post-activation in ethanol-activated oocytes were 21.9, 25.0, 47.4 and 32.6%. The cytoplasmic maturation leading to pronuclear formation upon activation increased when the I VM period was extended from 42 to 54h. When the activated oocytes were cultured for 96~120h to analyse early development of the activated oocytes, the rates of embryonic development upto 5~8 cell were 5.3, 5.8, 12.0 and 11.7% among the cultured embryos. The result indicate that earlier development of activated porcine occyte is dependent on the duration of oocyte maturation, and that better development could be obtained from the oocyte matured for 54h.

The seed spray technology prepares for landscape by spraying seeds, fertilizers and other materials but does not consider the relationship of the germination time and temperature of seeds for construction. The purpose of this study is to develop a functional formula that shows the relationship of germination and temperature for using the Papaver orientale L. seeds for the spray technology. The germination test in a chamber was progressed from March to April 2014 and the verification experiment proceeded in a greenhouse in May 2014. After culturing each 100 seeds of Petri dish and Papaver orientale L. seeds on filter beds 4 repetitive times, each 10mL of the first distilled water was sprayed to treat temperature at level 7 and the light source was maintained at 4~4.5μmoles/m2/s. The germination rate was investigated at two-day intervals, and the germination was based on the emergence of 3mm radicles. For experimental verification, seeding was implemented in a greenhouse and daily mean temperatures and germination periods were investigated. As a result of the treatment, based on a 50% germination rate, the functional relation between the temperature and germination period was found to be y = 1/-0.0004x²+ 0.0224x－0.0398, R2 = 0.9441. As the result of the analysis of the cardinal temperature from using graphs of functions, the lowest temperature was 2°C and the optimum temperature was 28°C and the highest temperature was 54°C, but in the actual test, normal germination was made at 5°C and the unusual germination of the final germination rate was below 50% at 35°C. In the experimental verification, as a result of substituting the daily mean temperature with functional formulas, it was found to be 4.4 days and the lapse of time for actual foliation was investigated to be 5.5 days.

논의 수문학적인 거동은 물관리에 따른 담수심 상태, 벼의 생육시기별 물꼬관리에 크게 지배 받는다. 강우사상에 의한 논에서의 저류 및 배수량은 하천유량에 적지 않은 영향을 준다. 본 연구에서는 홍수기 논의 저류효과를 정량적으로 파악하기 위하여 논의 저류량을 모의할 수 있는 일별 물수지 모형을 개발하고, 수원과 여주의 논을 대상으로 4년간(1996, 1997, 2001, 2002) 측정한 자료를 이용하여 모형의 적용성을 검토하였다. 모형에서 물꼬높이와 기준