Untargeted metabolomic fingerprinting is a discovery tool for the identification of metabolites associated with the response to dietary and environmental perturbations. Direct analysis in real-time mass spectrometry (DART MS) promises to be a powerful analytical technique for high-throughput metablome analysis of insect. In this study, we used the DART MS technique to find tracers related to the origin of small brown planthopper (Laodelphax striatellus), and conducted a untargeted metabolomic fingerprinting on the wings and exoskeleton in Chinese and domestic collectives. This paper showed that DART MS metabolomic fingerprinting represents a rapid and powerful analytical strategy enabling distinguish of two different origin’s small brown planthoppers by recording metabolomic fingerprints.

Phenotypic traits (physiological characteristics and somatic incompatibility) and genotypic traits (target region amplification polymorphism TRAP) were used to survey the diversity in Chinese Auricularia auricula systematically, which consisted of 32 main cultivated strains. The 27 important and stable physiological indexes were evaluated; somatic incompatibility test (SIT) reaction was described from three aspects: type, pigment, and intensity while intensity clustering alone revealed the phenotypic diversity among the 32 strains;16 pairs of TRAP primer combinations produced 535 unambiguous and reproducible DNA fragments, of these 524 (97.9%) were polymorphic. The phylogenetic trees were constructed by Unweighted Pair-group Method with Arithmetic Averages (UPGMA), which distributed the test strains into four to six major groups respectively. The principal coordinate analysis (PCO) of the three methods (physiological characteristics, SIT intensity and TRAP) exhibited highly similar clustering patterns, revealing that all the test strains can be divided into three groups considered significantly correlated with geographical regions. Results revealed the occurrence of relatively low diversity of A. auricula in the study. The cultivated strains in the same region are more similar physiologically and some strains are suspected to be synonymous. This study suggests that the value for estimating diversity are represented by TRAP＞SIT reaction intensity＞physiological characteristics in A. auricula, and PCO analysis can provided more effective and visible information than the UPGMA clustering dendrogram. Our finding will facilitate future germplasm resources management and breeding program of A. auricula in China.

최근 게임콘텐츠시장이 거대화됨에 따라, 게임콘텐츠시장은 게임개발자와 게임퍼블리서로 불리는 게임유통업자로 분리되어 게임시장을 활성화하고 있다. 하지만 게임콘텐츠의 불법복제 등으로 게임시장이 위축되고 있으며, 게임의 불법복제를 허용하거나 묵인하는 게임퍼블리서를 수색함으로써, 게임콘텐츠시장을 활성화할 수 있다. 디지털핑거프린 팅 기술은 멀티미디어 콘텐츠를 구매한 사용자가 불법으로 재배포하지 못하도록, 구매자 정보를 게임콘텐츠에 감지하 기 어렵도록 삽입하는 기술이다. 일반적인 워터마킹 기술과는 달리, 핑거프린팅을 파괴하기 위한 대표적인 공격방법 은 공모공격이며, 여러 명의 구매자가 자신들이 구매한 콘텐츠들을 평균, 또는 중간값을 취함으로써, 콘텐츠에 삽입되 어있는 구매자 정보를 교란시키는 방법이다. 본 논문에서는, 대규모사용자를 표시할 수 있는 코드북 설계기법과, LCCA에 강인한 AD (angular decoding) 기법을 포함하는 대용량 비디오 핑거프린팅 기법을 제안한다. 본 논문에서 제 안한 기법을 상용화된 게임 콘텐츠의 도입동영상에 적용하였으며, 평균, 중간값, 및 LCCA 공격에 강인함을 보였다. 다양한 WNR (watermark to noise ratio)에 대해 AD 기법을 실험한 결과, 정확한 공모자 추적이 이루어짐을 확인하였다

The multicolored Asian ladybird beetle, Harmonia axyridis, which demonstrates typical genetic polymorphism in its elytra color patterns. Early studies have color polymorphism in terms of geographical clines while a few investigated temporal populations in Coccinellidae. Nevertheless, note that geographical and temporal morph variation does not always correspond to what is expected from thermal and industrial adaption theories. A recent study of transformation and RNAi of the ladybird beetle, however, there is yet no evidence to indicate the variation is genetic or environmental factors. Here we describe a relatively new molecular fingerprinting technique, amplified fragment length polymorphism (AFLP). Because we think that color polymorphism in Coccinellidae is affected by genetic polymorphism. In total 38 markers were scored from which some markers were polymorphic. Supsequent UPGMA cluster analysis revealed 3 major group of Harmonia axyridis populations. But for strains that are more genetically similar, different primer combinations may be need to generate enough polymorphic marker.

Sorghum (Sorghum bicolor (L.) Moench) has been cultivated for cereal grain which has been traditionally used for steaming with rice in Korea. Various Korean sorghum varieties have been developed and distributed for farmers and consumers to meet their needs. Korean sorghum grains have been mostly sold at higher price in the market than sorghum grains imported from abroad. However, no varietal identification method was established to support fair trade in the cereal market. The objective of this study is to develop the identification method of Korean sorghum varieties using a multiplexed fingerprinting platform of SSR markers. One marker for the waxy allele and nine SSR markers were carefully selected based on their product sizes for the multiplexing. A robust multiplexed combination was revealed from serially designed experiments for the optimization of multiplex PCR. Five varieties and two elite breeding lines could be separated with their unique fingerprinting pattern from other sorghum individuals collected over the world. The platform separated most of individuals tested in this study, remaining three genotypes contained two or three identical individuals. The technique may be applied to detect closely-related individuals including full sibling progeny

Microsatellites are one of the most suitable markers for variety identification as it has great discrimination power for varieties with narrow genetic variation. The polymorphism level between forty microsatellite primer pairs and 148 soybean varieties was investigated through fluorescence based automatic detection system. A set of 16 primer pairs showed highly reproducible and polymorphic in these varieties. A total of 204 alleles were detected by using 16 microsatellite markers. The number of alleles per locus ranged from 6 to 28 with an average of 12.75 alleles per locus. The average polymorphism information content (PIC) was 0.86 ranging from 0.75 to 0.95. Two hundred four microsatellite loci were used to calculate Jaccard’s distance coefficients for unweighted pair group method using the arithmetic averages cluster analysis. These varieties were separated into several distinctive groups corresponding to varietal types. All of the varieties were perfectively discriminated by markers genotypes. This information may be useful to compare through genetic relationship analysis between existing and candidate varieties in distinctive tests and protection of plant breeders’ intellectual properties rights through variety identification.

Mulberries have importance in the sericulture industry as food for Bombyx mori, silkworm reared for its silk. Korean Morus alba have many cultivars and, for the protection of these cultivars and for utilization in plant-breeding programs, genetic information and the diversity among cultivars are essential. This study with 14 mulberry genotypes was undertaken using RAPD and ISSR fingerprinting to discover the genetic divergences between cultivars. Polymorphism rate among the cultivars produced by RAPD primer was found to be 64.48% and 66.29% relative to ISSR primer. The genetic relationships among the cultivars were identified using a dendrogram constructed with the UPGMA clustering method. Nei's method was used to calculate the genetic dissimilarity coefficients between each pair of genotypes, and the highest dissimilarity coefficient of 0.246 was exhibited between Suwon and Hwanggum cultivars. To determine the efficiency of each primer, a polymorphic index was calculated, and the robustness of the dendrogram was checked using cophenetic correlation coefficient. The results of this study can be utilized for the improvement of mulberry varieties in plant-breeding programs.

The purpose is to study the identification method of Huangjinju powder for injection and the medicinal materials by the fingerprint off-ray Diffraction Fourier (XRDF). We used the same method on both the studying of Huangjinju and the medicinal materials. Then we selected a few components alignment to compare. We analyzed the data by setting up the deviation d(a) as ±0.05 to calculate the rate of special mark on the sample (Px) and on the patent (P). The special XRDF of Huangjinju[d(a)/(I/I0)] have 5 peaks that have not expressed in medicinal materials. Therefore Px is 22.73%. Flos Trollii Chinensis has 3 special marks and Px is 17.65%. Flos Chrysanthemi Indici has 1 special mark and Px is 3.57%. Its coincided interplanar spacing with the patent is 2.907a. Flos Lonicerae Japonicae has 6 special marks and Px is 23.08%. Its special mark in the patent are 4.95/14 and 4.50/15, respectively. The P is 9.09%. Its coincided interplanar spacing with the patent is 2.910a and 3.05a, respectively. The number of special XRDF mark peaks of baicalin is 9 and Px is 18.37%. Its coincided interplanar spacing with the patent is 2.910a. It has visible mark and specificity adopting XRDF fingerprint to identify Huangjinju and medicinal materials. Establishing the quality standard is a synthetic index that depends both on special marks in the medicinal materials of the patent and on the coincidence peak data.

보리 국가목록등재 48품종의 hordein 밴드패턴의 다양성을 보고 이러한 hordein 밴드패턴을 D/B화하여 기존(참고)품종으로 관리, 활용하면서 품종구별 및 출원품종과 비교될 대조품종의 선별 가능성에 대하여 검토한 결과를 요약하면 다음과 같다. 보리 48품종의 hordein SDS-PAGE 결과, 총 22개의 hordein polypeptide밴드를 읽을 수 있었으며 다양한 15종류 의 hordein 밴드패턴을 볼 수 있었다. Hordein 밴드패턴에 따라 48품종을 집괴분석한 결과, 전체 유사도지수 0.54∼1.00 범위에서 3개군으로 분류되었고 주로 보리의 조성에 따라 분류되었다. Hordein polypeptide 밴드패턴 자체가 보리품종의 형태적 특성을 나타내는 유전적거리와 반드시 비례하는 것은 아니므로 현재의 UPOV관점과 마찬가지로 품종보호권 설정에 직접적인 근거를 제공할 순 없다고 판단되었지만 hordein polypeptide 밴드패턴 그 자체를 신품종의 구별성에 대한 보완적 자료로 이용할 수 있으리라 사료되었다.

Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Present study was conducted to evaluate the genetic diversity within a diverse collection of rice accessions used for Korean breeding programs. Two hundred eighty-seven rice cultivars, composed of temperate japonica, tropical japonica, indica, and Tongil-type of Korean crossing parents were evaluated by means of 15 simple sequence repeat (SSR) markers. A total of 99 alleles were detected, and the number of alleles per marker ranged from 4 to 11, with an average of 6.6 per locus. Polymorphism information content (PIC) for each of the SSR markers ranged from 0.2924 to 0.8102 with an average of 0.5785. These results, with the result that use of only 15 SSR markers made all rice cultivars examined could be uniquely distinguished, imply the efficiency of SSR markers for analysis of genetic diversity in rice. Cluster analysis was performed on similar coefficient matrics calculated from SSR markers to generate a dendogram in which two major groups corresponding to japonica (Group I) and indica and Tongil type rice (group II) with additional subclasses within both major groups. The narrowness of the Korean breeding germplasm was revealed by the fact that most of the Korean-bred and Japan-bred temperate japonica cultivars were concentrated into only 2 of the sub-group I-1 (143 cultivars) and I-2 (58 cultivars) among six sub-groups in major group of japonica. This is because of the japonica accessions used in this study was a very closely related ones because of frequent sharing of the crossing parents with similar genetic background with synergy effect of the inherited genetic difference between indica and japonica. A rice breeding strategy with the use of molecular markers was discussed for overcoming of genetic vulnerability owing to this genetic narrowness.

Genetic diversity of 31 rice varieties including 25 japonica and 6 indica varieties was evaluated using a combination of 19 microsatellite or simple sequence repeats (SSRs) and 28 random decamer oligonucle-otide primers. All 19 microsatellite primer sets representing 19 loci in the rice genome showed polymorphisms among the 31 varieties and revealed 91 alleles with an average of 4.80 bands per primer. Also all 28 random decamer primers used were informative and generated 114 non-redundant bands with a mean of 4.07 bands. Microsatellite markers detected higher number of alleles than random primers .although the mean difference was not statistically significant. A cluster analysis based on Nei＇s genetic distances calculated from the 205 bands resolved the 31 varieties into two major groups that correspond to indica and japonica subspecies, which is consistent with the genealogical information. As few as six random decamer primers or a combination of one microsatellite and four random decamer primers were sufficient to uniquely differentiate all 31 varieties. These combinations would be potentially useful in rice variety protection and identification considering that 25 out of 31 varieties used in this study are japonica rices with high grain quality and have close make up.