For experimental studies on the production of MR fluid for MR dampers, MR fluid with a viscosity of 506 cP and a density of 2.6 g/cc was produced. In order for MR fluid to have suitable performance for MR dampers, it is important to select surfactants, magnetic particles, base oil, and characteristic additives to obtain high damping force and maintain physical and chemical properties. In order to investigate the redispersibility of MR fluid, viscosity, density, saturation magnetic flux density, dispersibility, and temperature effects were evaluated. A particle size distribution meter and a vibrating magnetometer were used, and a yield stress and redispersion device were developed to obtain the yield stress of the MR fluid. The recovery rate of MR fluid was approximately 97% at 0.2% succinic acid coating and 8% anti-settling agent. And when current is applied, the viscosity increases by more than 90% due to magnetic properties.
Fetal Bovine Serum (FBS) plays a crucial role in animal cell culture; however, the increasing number of bovine fetuses used and sacrificed solely for FBS collection has raised ethical concerns globally. The welfare of fetuses during FBS blood collection has become a key focus of debate among animal welfare and ethics organizations worldwide. Previous studies indicate that heat-inactivated coelomic fluid (HI-CF) from the earthworm Perionyx excavatus may serve as a viable FBS alternative in adherent cell cultures. This study evaluates the potential of HI-CF as an FBS substitute during the in vitro maturation (IVM) stage of bovine embryo culture, with a focus on improving developmental rate through antioxidation effects. In this study, 2% HI-CF was incorporated into IVM media, assessing its impact on cell growth, differentiation, and the expression of genes related to antioxidation. The group of 2% of HI-CF exhibited a trend toward increased cleavage and blastocyst development rates compared to the control group. Although antioxidant genes such as NRF2 and GSR showed no statistically significant differences between the control and treatment groups, a trend toward increased expression was observed. Conversely, GPX1 displayed a trend of decreased expression. Notably, IGF1 and NQO1 were significant upregulated (p < 0.05) in the 2% HI-CF group. Additionally, oocytes stained with H2DCFDA showed a significantly reduced ROS levels (p < 0.05) in the 2% HI-CF group compared with controls. These findings suggest that HI-CF's antioxidative effects support enhanced cell growth and blastocyst development rate, surpassing those observed with FBS. Consequently, HI-CF shows promise as an effective alternative to FBS in vitro maturation of bovine oocytes.
The heat transfer characteristics of double-pipe spiral heat exchanger using aluminum oxide nano-fluid were investigated by three different sizes of curvature size, experimentally. Five concentration of nano-fluid as working fluid were made and tested to analyze the heat transfer characteristics. As results, the heat transfer performance was improved at 0.25% of nano-fluid due to high thermal conductivity, however, as the concentration of nanofluid increased (~2.0%), the heat transfer performance deteriorated due to the increase in thermal resistance caused by the sedimentation of particles in the flow path. In addition, the nano-fluid has a higher pressure drop than water due to its high density and viscosity. The optimal range for heat transfer enhancement of nano-fluid was found to be less than 4.0 LPM in flow rate and 0.25% of nano-fluid concentration in this study.
In order to understand the MR fluid flow in the MR damper core, the annular orifice path was simplified into a square channel and the electromagnetic flow was analyzed. For this purpose, the CFD-ACE+ program was used. The temperature and magnetic field of the MR fluid were based on room temperature and orifice wall data, and 2-D steady incompressible laminar flow was assumed. The inlet and outlet of the orifice channel are at atmospheric pressure, and the inflow velocity of the MR fluid is 0.1 m/s. After analyzing the magnetic field of the core, which is a simple model of the 1 stage MR damper, the electromagnetic flow analysis of the MR fluid flowing through the orifice channel was performed. From this, the magnetic field of the orifice channel and the electromagnetic flow of the MR fluid were observed. As the magnetic flux density increased, the flow distribution and velocity of the MR fluid in the channel core changed significantly.
In this study used Computational Fluid Dynamic analysis to examine NOx reduction in hydrogen combustion, analyzing six conditions with varying air/fuel ratios, temperatures, and concentrations. Results were compared between two combustor shapes and previous experimental data. Findings showed increased air/fuel ratios decreased flame temperature and increased post-combustion O2. NOx emissions peaked at high temperatures and low O2. Numerical results aligned with previous experimental trends, validating the approach. Combustor shape differences, reflecting variations in fuel and air pipes, significantly affected flow rates and combustion positions. This reduced NOx emissions up to a certain air/fuel ratio, but excessive increases diminished this effect. The study highlights the complex relationship between combustor design, operating conditions, and NOx emissions. Further research is needed to optimize NOx reduction by considering pipe numbers and combustion locations. Future studies should explore various combustor geometries, fine-tune air/fuel ratios, and investigate additional parameters influencing NOx formation and reduction in hydrogen combustion systems.
Background: Porcine embryonic development is widely utilized in the medical industry. However, the blastocyst development rate in vitro is lower compared to in vivo . To address this issue, various supplements are employed. Extracellular vesicles (EVs) play the role of communicators that carry many bioactive cargoes. Additionally, the contents of EVs can vary on the estrous cycle. Methods: We compared the effects of adding EVs derived from porcine uterine fluid (UF), categorized as non-EV (G1), EVs in estrus (G2) and EVs in diestrus (G3). After in vitro culture (IVC) was performed in three different groups, cleavage rate and blastocyst development rate were examined. In addition, glutathione (GSH) and reactive oxygen species (ROS) levels were measured 2 days after activation to assess oxidative stress. Results: Using NTA and cryo-TEM, we confirmed the presence of EVs with sizes ranging from 30 nm to 200 nm, that the particles were suitable for analysis for analysis. In IVC data, the highest cleavage rate was observed in G2, which was significantly different from G1 but not significantly different from the next highest, G3. Similarly, the highest blastocyst development rate was observed in G2, which was significantly different from G1 but not significantly different from the next highest, G3. Conclusions: These results indicate that estrus derived EVs contain biofactors beneficial for early blastocyst development, including GSH which protects the blastocyst from oxidative stress. Additionally, although diestrus-derived EVs are expected to have some effect on blastocyst development, it appeared to be less effective than estrus-derived EVs.
A cyclone separator is a device that separates solid particles from a fluid using centrifugal force and gravity in its inner chamber. Among cyclone separators, the separator that uses water as a working fluid is called as hydrocyclone separator, which has been developed for the purpose of dehydrating solid mixtures with a proportion of solids floating in liquids greater than 1, such as soil, coal, and cement slurry. In this paper, a hydrocyclone was designed based on the previously proposed design method, and how different the performance is from the targeted value was investigated using the computational fluid dynamics.
유체-구조물-지반 상호작용을 고려한 액체저장탱크의 유한요소 모형을 제시하고, 비선형 지진응답 해석기법을 정식화한다. 탱크 구조물은 기하 및 재료 비선형 거동을 고려할 수 있는 쉘 요소로 모델링한다. 유체의 거동은 acoustic 요소로 구현하고, interface 요소 를 사용하여 구조물과 결합한다. 지반-구조물 상호작용을 고려하기 위해 지반의 근역과 원역을 각각 solid 요소와 perfectly matched discrete layer로 모델링한다. 예제 20만 kl급 액체저장탱크의 지진취약도 해석에 적용하여, 유연한 지반에 구조물이 놓인 경우 부지에 서의 암반노두운동의 증폭 및 필터링으로 인해 지진취약도의 중앙값과 대수 표준편차가 감소하는 것을 관찰할 수 있다.
Recently, the demand for shape memory alloys in the biomedical industry is increasing. Nitinol alloy, which accounts for most of the shape memory alloy market, occupies most of the biomedical field. Nitinol for biomaterials requires a clean surface without sub-micron surface integrity and surface defects in order to be used more safely in a living body. Among them, new technologies such as polishing using MR fluid are being studied, but there is a disadvantage in that it takes a long time for processing due to a low material removal rate. In this study, material removal studies were conducted for effective polishing, and excellent polishing properties of MR fluid were confirmed.
This study performed the seismic response analysis of an LNG storage tank supported by a disconnected piled raft foundation (DPRF) with a load transfer platform (LTP). For this purpose, a precise analytical model with simultaneous consideration of Fluid-Structure Interaction (FSI) and Soil-Structure Interaction (SSI) was used. The effect of the LTP characteristics (thickness, stiffness) of the DPRF system on the seismic response of the superstructure (inner and outer tanks) and piles was analyzed. The analytical results were compared with the response of the piled raft foundation (PRF) system. The following conclusions can be drawn from the numerical results: (1) The DPRF system has a smaller bending moment and axial force at the head of the pile than the PRF system, even if the thickness and stiffness of the LTP change; (2) The DPRF system has a slight stiffness of the LTP and the superstructure member force can increase with increasing thickness. This is because as the stiffness of the LTP decreases and the thickness increases, the natural frequency of the LTP becomes closer to the natural frequency of the superstructure, which may affect the response of the superstructure. Therefore, when applying the DPRF system, it is recommended that the sensitivity analysis of the seismic response to the thickness and stiffness of the LTP must be performed.
Passengers on public buses operating in the metropolitan area are exposed to the closed indoor air for minutes to hours. The indoor air quality of buses is mostly controlled through ceiling-mounted ventilation and filtration devices. A simulation study using a commercial code was conducted for fluid flow analysis to evaluate the potential effectiveness of an air purifier that can be inserted into bus windows to supply clean air from the outside to the inside. As a result of field measurements, the average CO2 concentration inside the bus during morning and evening rush hours ranged from 2,106±309 ppm to 3,308 ± 255 ppm depending on the number of passengers on board. This exceeded the Guideline for Public Transportation. The optimal installation position of an air purifier appeared to be the front side of the bus. In fact, even a low diffusing flow velocity of 0.5m/s was effective enough to maintain a low concentration of CO2 throughout the indoor space. Based on numerical analysis predictions with 45 passengers on board, the maximum CO2 concentration in the breathing zone was 2,203 ppm with the operation of an air purifier.
Owing to the great demand for portable and wearable chemical sensors, the development of all-solid-state potentiometric ion sensors is highly desirable considering their simplicity and stability. However, most ion sensors are challenged by the penetration of water and gas molecules into ion-selective membranes, causing unstable and undesirable sensing performances. In this study, a hydrophobic ionic liquid-modified graphene (Gr) sheet was prepared using a fluid dynamics-induced exfoliation and functionalization process. The high hydrophobicity and electrical double-layer capacitance of Gr make it a potential solid-state ion-to-electron transducer for the development of potentiometric sodium-ion ( Na+) sensors. The as-prepared Na+ sensors effectively prevented the formation of the water layer and penetration of gas species, resulting in stable and high sensing performances. The Na+ sensors showed a Nernstian sensitivity of 58.11 mV/[Na+] with a low relative standard deviation (0.46), fast response time (5.1 s), good selectivity (K < 10− 4), and good durability. Furthermore, the Na+ sensor demonstrated its feasibility in practical applications by measuring accurate and reliable ion concentrations of artificial human sweat and tear samples, comparable to a commercial ion meter.
The importance of urban green space creation is increasingly recognized as the most realistic and efficient approach for fine dust mitigation in urban areas. Particularly considering the characteristics of domestic cities, the application of buffer green spaces along roads can maximize the efficiency of fine dust reduction without the need for separate green space creation. Accordingly, this study analyzed the fine dust mitigation effects based on the types of plantings in the central dividers and roadside trees in Jeonju City, Jeollabuk-do. To do this, we controlled various external variables of urban space and considered the planting arrangement types in the central dividers, carrying out the analysis using a CFD simulation. The simulation results confirmed that the central dividers with plantings demonstrated more effective ultrafine dust reduction than those without. Moreover, the arrangement of roadside trees showed a greater ultrafine dust reduction effect when adopting a multilayered structure compared to a single layer. Based on these findings, we concluded that installing both trees and shrubs simultaneously in the central dividers and along roads was effective for ultrafine dust mitigation. On this basis, we quantified the dust reduction effects of plants in urban street environments and proposed planting guidelines for roadside green spaces to improve air quality.
In the fluid-structure interaction analysis, the finite element formulation is performed for the wave equation for dynamic fluid pressure, and the dynamic pressure is defined as a degree of freedom at the fluid nodes. Therefore, to connect the fluid to the structure, it is necessary to connect the degree of freedom of fluid dynamic pressure and the degree of freedom of structure displacement through an interface element derived from the relationship between dynamic pressure and displacement. The previously proposed fluid-structure interface elements use conformal finite element meshes in which the fluid and structure match. However, it is challenging to construct conformal meshes when complex models, such as water purification plants and wastewater treatment facilities, are models. Therefore, to increase modeling convenience, a method is required to model the fluid and structure domains by independent finite element meshes and then connect them. In this study, two fluid-structure interface elements, one based on constraints and the other based on the integration of nonsmooth functions, are proposed in nonconformal finite element meshes for structures and fluids, and their accuracy is verified.
This study analyzed the flow inside floating seedling equipment for Scapharca subcrenata. Due to the aging society of fishing villages, it is impossible to continuously input the labor force. Therefore, it is necessary to improve efficiency. Scapharca subcrenata has high per capita consumption. It serves as an important aquatic food resource. Scapharca subcrenata culture tends to be highly dependent on the natural environment. Production of Scapharca subcrenata is difficult to predict with low stability. In the past, manpower directly installed bamboo nets in mudflats. The seedling equipment devised in this study is a floating type and can be freely moved on the sea according to the prediction of Scapharca subcrenata generation. The flow around the floating seedling equipment was analyzed by numerical analysis. The physical phenomena of the flow around the net inside the floating seedling equipment were visualized. As a result, the space between the floating seedling equipment and the bottom net and the space between the net groups showed a lower flow rate than the inlet flow rate. It is expected that the low flow rate of the floating seedling equipment will have a positive effect on the attachment of Scapharca subcrenata.