Previously, we found that expression by translational fusion of the polyhedrin (Polh)-green fluorescence protein (GFP) led to the formation of granular structures and these fluorescent granules were easily precipitated by high-speed centrifugation. Here, we developed an easy, fast, and mass purification system using this baculovirus expression system (BES). An enhanced GFP (EGFP) fused with Polh gene at the N-terminus including an adaptor and enterokinase (EK) site between Polh and EGFP was expressed in Sf9 cells. The cells infected by AcPolhEKA-EGFP produced fluorescent granules. The EGFP fusion protein was purified from granule-containing cells according to three steps; cell harvest, sonication and EK digestion. Through the final enterokinase digestion, EGFP was presented mainly in the supernatant (93.3%) and the supernatant also showed a pure EGFP band. These results suggest that the combined procedure of Polh fusion expression and enterokinase digestion can used for the rapid and easy purification of other proteins.

오늘날 디지털컨버전스(Digital Convergence)시대에 3D그래픽은 산업간, 장르간 융합을 주도하는 촉진제 역할을 하는 중요한 역할을 하고 있다. 영상제작에서도 기존의 장르 간 구분에서 벗어나 교류와 융화라는 탄력적인 시각과 다양한 시도들이 필요하다. 본 연구에서는 게임 3D그래픽의 로우폴리곤방식이 프리프러덕션의 프리비주얼 작업 등으로 역할이 확대 되고 있음을 보여주고 있다. 특히 상대적으로 프리프러덕션 단계가 빈약한 국내 영상 산업에서 3D그래픽은 질적 향상을 이끌어낼 수 있는 새로운 제안이 될 것이라고 본다.

In this age of information ruled by new technologies and knowledge, the world is interconnected as a single community, and within this trend of globalization, new cultural codes are emerging through temporal fusion between the past and the present and spatial fusion between different regions and countries. In this situation, it seems meaningful to review Korean fusion foods and restaurants serving such foods, as well as to consider their future directions. Thus, the objective of the present study was to survey and analyze Korean fusion restaurants representing Korean culture not only in Korea, but also in foreign cities, and to identify the expression characteristics of such restaurants. Based on restaurants recommended in relevant magazines and on Internet sites, 18 spaces were selected, visited, and surveyed, in which tradition and modernism were well-mixed. Data on the shapes, materials, colors, and patterns of spatial elements and food-related elements, including photographs, were collected and analyzed. The results are as follows. Of the 18 restaurants, 13 (72%) showed temporal fusion in both spatial and food-related elements, 4 showed temporal fusion in spatial elements and cultural fusion in food-related elements, and 1 showed cultural fusion in both spatial and food-related elements. In general, the spaces were mainly designed with modern elements and partially with traditional elements (ceilings, windows, furniture, articles), and the fusion of food-related elements was made in diverse forms that included temporal fusion restructuring traditional menus contemporarily, and cultural fusion harmonizing traditional food with Western cookery.

In the viewpoint of engineering, materials problem is a key problem, which determines whether the exploitation of fusion energy will be success. The most important class of fusion materials is plasma-facing materials (PFM). W, as high Z high melting-point metal is one of the most important candidate materials due to its high plasma erosion resistance. Improving the ductility of W and W based alloy, lowering its ductile-brittleness transition temperature for meeting the requirements of fusion application is an important task. In this paper, severalpowder meatllurgy methods of fabricating W and W based materials are being investigated.

본 연구는 한우 성체 유래 귀세포(Korean bovine ear skin fibroblasts, KbESF)와 태아 섬유아세포(Korean bovine fetal fibroblasts, KbFF)를 이용한 체세포 복제(SCNT) 시 세포종류, 배양기간 그리고 융합방법이 핵이식 수정란의 발달에 미치는 영향을 알아보기 위하여 실시하였다. 태아 섬유아세포는 임신 51일령의 한우태아에서 분리하였고, 귀세포는 28개월령의 성우의 귀에서 채취하였다. 세포는 15주 동안 체외에서 배양하며 체세포 핵이식(SCNT)에 공시하였다. 융합방법을 비교하기 위해 챔버방법과 전극 바늘을 이용한 방법으로 핵과 세포질을 융합하였다. 세포의 doubling time은 KbFF에서 17.3시간, KbESF에서 24.3시간으로 나타났다. 핵이식 후 융합과 분할율은 needle 방법에서 보다 유의적으로 높았으나(각 각 76.1과 81.2%, P<0.05), 배반포 발달율은 차이가 없었다. KbESF의 경우, 배반포 발달율은 passage 5~9(39.4%)와 13~15(40.4%)에서 passage 1~4에 비하여 유의적으로 높았다(P<0.05). KbFF의 경우, 융합율은 passage 5~8과 13~15에서 각각 75.0 및 76.8%로 passage 1~4(61.5%)보다 높았으나, 난분할율과 배반포 발달율은 차이가 없었다. 결론적으로, SCNT 수정란의 발달은 융합 방법에 의해 영향을 받을 수 있으나, 계대배양 15회까지 장기배양을 한 경우는 복제수정란의 발육에 영향을 주지 않는 것으로 판단된다.

This study was conducted to test whether the transgenic cattle pass the transgene to their progeny through germ cells, and whether the transgene is expressed in the mammary gland of ransgenic cows. Two male ransgenic calves were born from IVF-derived embryos injected with bovine β-casein/human lactoferrin fusion gene and then grew up to be reproducible. Semen was collected from a transgenic bull after 18 mon of age and then frozen. Bovine oocytes matured in vitro were fertilized with spermatozoa of the transgenic bull and cultured in 50㎕ drops of CRlaa medium supplemented with 3 mg/mL BSA. After 48 h of culture, cleaved embryos were determined for the presence of transgenes by DNA polymerase chain reaction (PCR). Proportion of transgene positives among bovine embryos fertilized with sperm of the transgenic bull was 20.9% (28/134). One of transgenic bulls did not produce transgenic sperm. Out of 34 calves produced from recipient heifers inseminated with semen of the other bull, 3 (8.8%) were transgenic animals (2 females and 1 male). Thus, one transgenic bull showed a low transmission frequency below Mendelian levels in both the IVF-derived embryos and his progeny. It was demonstrated by Southern blot that copy numbers of the transgene in the transgenic progeny enhanced about 1.8 times as compared to those of the founder bull The results demonstrate that the transgenic bull carrying human lactoferrin gene could pass his transgene to the progeny through germ cells, although he is a germ-line mosaic.

Fuzzy information representation of multi-source spatial data is applied to landslide hazard mapping. Information representation based on frequency ratio and non-parametric density estimation is used to construct fuzzy membership functions. Of particular interest is the representation of continuous data for preventing loss of information. The non-parametric density estimation method applied here is a Parzen window estimation that can directly use continuous data without any categorization procedure. The effect of the new continuous data representation method on the final integrated result is evaluated by a validation procedure. To illustrate the proposed scheme, a case study from Jangheung, Korea for landslide hazard mapping is presented. Analysis of the results indicates that the proposed methodology considerably improves prediction capabilities, as compared with the case in traditional continuous data representation.