본 연구는 검거세미밤나방(Agrotis ipsilon) 성페로몬 트랩에 혼재하여 유살되는 은무늬밤나방아과 형태적 분류와 동정법 수립을 위해, 날개 무늬의 형태계측학 분석을 실시하였다. 은무늬밤나방아과 개체는 2023년 11월 부터 12월까지 제주도 애월읍 일대에서 채집되었으며, 콩 해충으로 알려진 콩은무늬밤나방(Ctenoplusia agnata) 을 비롯하여, 다양한 농작물을 가해하는 것으로 알려진 붉은금무늬밤나방(Chrysodeixis eriosoma)의 수컷 성충이 포획되었다. 앞날개의 형태 및 무늬를 가지고 현장에서 쉽게 동정할 수 있는 형태적 특징을 도출하기 위하여, 각 성충 개체의 앞날개를 잘라 현미경 카메라로 촬영하고, 앞날개의 내횡선, 아외연선, 반점 크기 등 15개의 형질 을 측정하였다. 또한 각 형질 간의 상관관계를 분석하였으며 빈도분포를 통하여 두 종간 분리되는 형질을 파악하 였다. 최종적으로 다변량 분석법을 적용하여 두 집단이 어떻게 군집을 이루는지 분석하고, 날개형태만으로 붉은 금무늬밤나방과 콩은무늬밤나방을 구분할 수 있는 방안을 제시하였다.

식물에 전기장을 처리하면 식물의 생장속도가 빨라지거나 영양학적으로 긍정적인 변화가 생긴다고 알려져 있다. 최근 음이온 처리 시 식물에 전기장을 처리한 것과 유사한 효과가 나타난다고 보고되었고 본 연구에서는 이러한 음이온을 온실해충인 점박이응애와 목화진딧물에 처리하여 방제효과 여부를 확인하였다. 그 결과 음이 온 처리 시 점박이응애와 목화진딧물에서 살충효과와 기피효과가 나타났다. 또한, 점박이응애 알에서도 음이온 처리가 부화율에 영향을 주는 것을 확인할 수 있었다. 이러한 시험 결과를 바탕으로 온실에서 밀도실험 결과, 700,000 ion/cm3 농도에서 무처리구에 비해 밀도가 감소함을 확인할 수 있었다. 따라서, 본 연구는 음이온 처리 시, 부가적인 효과로 온실해충(점박이응애, 목화진딧물)에 대해 친환경적 방제 가능성을 보여준다.

Ecosystems provide various ecosystem services based on biodiversity. However, biodiversity is facing crises due to anthropogenic factors such as pollution, land use change, and climate change. Threats to biodiversity can significantly impact the provision and stability of ecosystem services, extending beyond simple species decline. To address threats to biodiversity, it is crucial to evaluate how anthropogenic factors affect not only biodiversity but also ecosystem services. This study aims to investigate the energy flux in a post-mining area based on the biodiversity of soil ecosystems and assess its suitability as an evaluation metric. It was observed that as the concentration of the primary pollutant, arsenic, increased, both the biomass of soil organisms and energy flux decreased. Furthermore, soil ecosystem multifunctionality may be negatively affected by pollution. These findings contribute to understanding the impact of pollution on soil ecosystem biodiversity and energy flux in post-mining areas and provide important information for more effective conservation and management of ecosystem services.

목적 : 본 연구는 Orthokeratology(OK) 렌즈와 기존의 단안렌즈(SV) 안경을 착용한 근시 청소년의 굴절이상을 분석하여 OK 렌즈가 근시 진행에 미치는 영향을 살펴보고자 하였다. 방법 : 연구방법은 중국 길림성 북화대학부속병원 안과를 방문한 청소년 환자 100명을 대상으로 50명의 환자를 OK렌즈군과 단초점 안경군으로 나누었다. 두 그룹의 환자 모두 1년 동안 추적관찰하여 시력(VA), 구면등가굴절 (SE), 안축 길이(AL), 각막 내피 세포 수(CEC), 중심 각막 두께(CCT) 및 압력(IOP)의 변화를 비교하였다. 통계분 석은 SPSS 소프트웨어를 이용하여 독립표본 T-test로 데이터를 분석하였으며, p<0.050을 통계적으로 유의한 것 으로 간주하였다. 결과 : Euclid Orthokeratology(OK)렌즈를 착용한 환자의 시력이 서서히 향상되었으나, 단초점 안경을 착용한 환자들은 시력이 감소하는 추세를 보였으며, 두 그룹 간의 차이는 통계적으로 유의하였다(p<0.010). 굴절검사 결과 는 OK 렌즈를 착용한 후 굴절이상이 유의하게 감소하였으며, 안경을 착용한 환자들은 굴절이상이 증가한 것으로 나타났다. 두 그룹 간의 차이는 통계적으로 유의하게 증가하였다(p<0.010). 또한, 안축 길이의 분석 결과, OK 렌 즈를 착용한 환자들의 안축 길이는 기준값 대비 0.18±0.18 mm 증가하였고, 안경을 착용한 환자들의 안축 길이는 기준값 대비 0.61±0.20 mm 증가하였다. 두 그룹 간의 차이는 통계적으로 유의하게 증가하였다(p<0.001). OK 렌즈를 착용한 환자들은 안압, 각막 내피 세포 수 및 중심 각막 두께에서 기준값 대비 유의한 변화가 없었다 (p>0.050). 결론 : 안경 대비 각막교정렌즈가 구면 등가 구면굴절력(SE)를 감소시키고 시력을 향상시켰으며, 안축 연장을 늦추는데 더 효과적임을 알 수 있었다.

Silage inoculants, crucial in modern silage production, comprise beneficial microorganisms, primarily lactic acid bacteria (LAB), strategically applied to forage material during ensiling. This study aimed to compare the effectiveness of various inoculants produced by different companies. Five treatments were evaluated, including a control group: T1 (Lactobacillus plantarum), T2 (Lactobacillus plantarum + Pediococcus pentosaceus), T3 (Lactobacillus plantarum + Pediococcus pentosaceus + Lactobacillus buchneri), T4 (Lactobacillus plantarum + Lactobacillus acidophilus + Lactobacillus bulgaricus), and T5 (Lactobacillus plantarum + Pediococcus pentosaceus + Enterococcus faecium). Italian ryegrass was harvested at the heading stage and treated with these silage inoculants. Samples were collected over a 60-day ensiling period. Co-inoculation with L. plantarum and P. pentosaceus (T2) resulted in significantly higher CP compared to the control group co-inoculation exhibited with resulted in Lactobacillus plantarum and Pediococcus pentosaceus in the T2 treatment exhibited higher CP content of 106.35 g/kg dry matter (DM). The T3 treatment, which included heterofermentative bacterial strains such as Lactobacillus buchneri, exhibited an increase in acetic acid concentration (11.15 g/kg DM). In the T4 treatment group, which utilized a mixed culture of Lactobacillus acidophilus and Lactobacillus bulgaricus, the NH3-N/TN content was observed to be the lowest (20.52 g/kg DM). The T5 containing Enterococcus faecium had the highest RFV (123) after 60 days. Expanding upon these findings, the study underscores not only the beneficial effects of particular inoculant treatments on silage quality but also underscores the potential of customized inoculation strategies in maximizing nutrient retention and overall silage preservation.

Background: The biological information of fish, which include reproduction, is the prerequisite and the basis for the assessment of fisheries. Methods: The aim of this work was to know the reproductive biology with the first sexual maturity (TL50) and the spawning period for 58 mainly fish species in the waters around La Réunion Island (Western Indian Ocean). Twenty families belonging to the Actinopterygii were represented (acanthuridae, berycidae, bramidae, carangidae, cirrhitidae, gempylidae, holocentridae, kyphosidae, labridae, lethrinidae, lutjanidae, malacanthidae, monacanthidae, mullidae, polymixiidae, pomacentridae, scaridae, scorpaenidae, serranidae, sparidae; 56 species; n = 9,751) and two families belonging to the Elasmobranchii (squalidae, centrophoridae; 2 species; n = 781) were sampled. Between 2014 and 2022, 10,532 individuals were sampled covering the maximum months number to follow the reproduction periods of these species. Results: TL50 for the males and the females, respectively, ranged from 103.9 cm (Acanthurus triostegus ) to 1,119.3 cm (Thyrsitoides marleyi ) and from 111.7 cm (A. triostegus ) to 613.1 cm (Centrophorus moluccensis ). The reproduction period could be very different between the species from the very tight peak to a large peak covered all months. Conclusions: Most species breed between October and March but it was not the trend for all species around La Réunion Island.

Bolivian tuber species like potato (Solanum tuberosum), native potato (Solanum sp), Oca (Oxalis tuberosa Molina), Olluco (Ullucus tuberosus Caldas), and Isaño (Tropaeolum turosum Ruíz & Pav.) hold extraordinary nutritional value and cultural significance, particularly within the Andean region. This study examined the mineral composition of Bolivian tuber species as an essential step toward understanding their nutritional significance and potential contributions to addressing dietary deficiencies. The research involved detailed analysis of diverse tuber cultivars, uncovering distinct mineral profiles across species. Native potato shows high levels of nitrogen (N), potassium (K), phosphorus (P), and magnesium (Mg) levels, alongside moderate micronutrients like iron (Fe) and zinc (Zn). Commercial potatoes exhibited prominence in N, P, and K, with moderate Fe, Zn, and manganese (Mn) levels. Oca, Isaño, and Papa Lisa displayed unique mineral concentrations, offering potential nutritional benefits. Intricate correlations and significant variances among elements highlighted the diverse mineral compositions among these tuber species. Multivariate analyses emphasized distinct mineral profiles unique to each species, revealing significant compositions of isaño and papa lisa's. The Multitrait Genotype- Ideotype Distance Index (MGIDI) identified isaño jaspeado, isaño and an unnamed native potato, AXT2, as promising ideotypes due to their exceptional mineral compositions. These findings provide comprehensive insights into Bolivian tuber species' various mineral compositions, underscoring their nutritional significance and potential in targeted breeding for improved dietary support and enhanced food security.

Background: Hemiparesis not only affects the affected side but also exerts an impact on the unaffected side. Stroke patients endure muscular weakness attributable to hemiparesis, resulting in asymmetry of muscular strength between the paralyzed and non-paralyzed sides. Objectives: To investigate the correlation between lower limb muscle strength asymmetry and functional factors in patients with stroke. Design: A cross-sectional study. Methods: A total of 58 stroke patients participated in this study. After measuring the muscle strength of both lower limbs using manual muscle testing, the asymmetry was calculated. Functional factors such as berg balance scale (BBS), timed up and go (TUG), 10-metre walk (10MW), and modified barthel index (MBI) were measured. Results: The BBS showed positive correlations with hip, knee, and ankle strength asymmetry (P<.05). The TUG showed negative correlations with hip, knee, and ankle strength asymmetry (P<.05). The 10MW showed negative correlations with hip, knee, and ankle strength asymmetry (P<.05). The MBI showed positive correlations only with hip strength asymmetry (P<.05). Conclusion: We were found that there is a more pronounced lower limb muscle strength asymmetry in the lower extremity of stroke patients, which is associated with BBS, TUG, and 10MW.

This work involves the development of a novel waste-derived carbon dots (CDs) conjugated with silver (Ag) nanohybrid system-based Fluorescence Resonance Energy Transfer (FRET) sensor for the detection of melamine. CDs and Ag nanoparticles served as energy donors and energy acceptors, respectively. CDs were synthesized from orange peel waste through a combined hydrothermal and ultra-sonication route. The synthesized CDs had hydroxyl, amino, and carboxyl groups on their surface, explaining that waste-derived CDs can act as reducing and stabilizing agents and showed strong absorption and fluorescence emission at 305 and 460 nm, respectively. The bandgap, linear refractive index, conduction band, and valance band potential of CDs were observed to be 2.86, 1.849, 1.14, and 4.002 eV, respectively. No significant difference was observed in the fluorescence properties at different pH (acid and alkaline) and ionic concentrations. Given their fluorescent nature, the synthesized CDs were used for the detection of melamine. The fluorescence of CDs was found to be quenched by Ag+ due to the FRET energy transfer between CDs to Ag. Notably, the zeta potential of Ag@CDs was changed from − 28.7 mV to − 30.6 mV after the incorporation of Ag+. Ag@CDs showed excellent selectivity and sensitivity toward the sensing of melamine in the aqueous solutions with the limit of detection ~ 0.85 μM. Increasing the melamine level also raises the FL intensity of Ag@CDs. The substrate was effectively used in the detection of melamine in milk as a real application and the recovery percentage was found to be 98.03%. Moreover, other adulterants such as urea and formaldehyde can be detected selectively by Ag@CDs. Overall, the synthesized Ag@CDs can be used as an efficient material for sensing applications involving such food adulterants.

Cellulose has experienced a renaissance as a precursor for carbon fibers (CFs). However, cellulose possesses intrinsic challenges as precursor substrate such as typically low carbon yield. This study examines the interplay of strategies to increase the carbonization yield of (ligno-) cellulosic fibers manufactured via a coagulation process. Using Design of Experiments, this article assesses the individual and combined effects of diammonium hydrogen phosphate (DAP), lignin, and CO2 activation on the carbonization yield and properties of cellulose-based carbon fibers. Synergistic effects are identified using the response surface methodology. This paper evidences that DAP and lignin could affect cellulose pyrolysis positively in terms of carbonization yield. Nevertheless, DAP and lignin do not have an additive effect on increasing the yield. In fact, combined DAP and lignin can affect negatively the carbonization yield within a certain composition range. Further, the thermogravimetric CO2 adsorption of the respective CFs was measured, showing relatively high values (ca. 2 mmol/g) at unsaturated pressure conditions. The CFs were microporous materials with potential applications in gas separation membranes and CO2 storage systems.

This study aims to distinguish between various Ethiopian durum wheat varieties based on their genetic identity using chemical and morphological characterization of seeds. Combinatorial employment of five chemical tests on seeds showed marked qualitative variation among the test varieties, with high discriminatory potential noted for the standard phenol test, followed by the modified phenol and iodide tests. The modified phenol test was instrumental in further discriminating between the varieties that were not identified using the standard phenol test. Unlike the iodide and phenol tests, the NaOH and KOH tests did not show significant variation among the varieties. These results underscore the efficacy of phenol and iodide tests in differentiating between durum wheat varieties. Although the morphological traits were advantageous in seed characterization, they lacked discriminatory power compared with that of the chemical tests. This study concludes that a single test is inadequate for varietal discrimination; rather, a combination of chemical tests can augment the discriminatory potential.

산오이풀(Sanguisorba hakusanensis)은 한국의 자생식물 이며 정원소재로써 가치가 있지만, 생육 및 생리적 특성 및 정 원 적응 여부에 대하여 알려진 정보가 많지 않아 이용에 어려 움을 겪고 있다. 본 연구에서는 자생식물인 산오이풀의 관수 주기 및 NaCl 농도에 따른 생장, Fv/Fm, NPQ, 성분 변화, 무기성분 변화를 조사하여 내건 및 내염성 보유 여부, 생육 한 계 범위, 스트레스 환경에서 생육을 유지하기 위한 반응을 파 악하고자 했다. 실험 결과 NaCl 무처리구의 관수주기에 따른 성분 분석에서 엽록소 함량의 감소를 제외하고 유의한 차이가 나타나지 않았으나 이는 토양수분함량이 건조 스트레스를 유 발할 정도로 감소하지 않았기 때문으로 보인다. 염 처리에서 는 2주 이후 급격한 스트레스 반응이 나타났고 3주차부터 고 사하기 시작하여 6주차에 모든 개체가 최종 고사했다. 이러한 결과는 2주까지 염 스트레스에 의해 유발되는 2가지 스트레 스 중 초기에 나타나는 삼투 스트레스에는 저항하였으나 이후 나타나는 NPQ의 감소 등 이온 스트레스에 의해 유발된 광합 성 기구 붕괴로 인해 정상적인 생육을 유지할 수 없었기 때문 에 나타난 것으로 보인다. 그러나 무기이온 분석은 이온 스트 레스에 저항하기 위한 메커니즘의 존재 가능성을 시사하였다. 상대적으로 염 농도가 낮을 때에는 세포내 Ca2+ 및 K+ 수준이 높았는데, 이는 Ca2+ 수준이 높아짐에 따라 Na+를 세포 밖으 로 방출시키는 단백질, Na+를 K+와 함께 수송하는 단백질이 기능하여 Na+축적을 지연시키는 반응이 있었음을 시사한다. 그러나 NaCl을 고농도로 처리했을 때는 이러한 반응이 관찰 되지 않았다. 따라서 산오이풀은 염 스트레스에 의해 야기되 는 삼투 스트레스에 강한 저항성을 가지고 있고 이온 독성을 줄이기 위한 메커니즘으로 Na+ 세포내 축적을 지연시키는 것으로 보이지만, 심한 염 스트레스를 받았을 때 나타나는 급격 한 반응에서 이러한 메커니즘이 기능하지 못하고 이온독성에 매우 취약한 것으로 여겨진다. 본 연구를 통해 자생식물인 산 오이풀의 활용을 늘리는 데 기초적인 자료를 제공할 수 있을 것으로 생각된다.

충청남도농업기술원 화훼연구소에서 2021년에 개발된 ‘Pinky Lang’은 분홍색 홑꽃 타입의 스프레이 국화 신품종 이다. ‘Pinky Lang’은 2016년 10월에 육종 하우스 내에서 2016년에 순백색의 진한 녹심이 매력적인 품종으로써 선발 된 백색 홑꽃의 ‘Bodre’ 품종을 방임 수분하여 48개의 종자 를 채취했다. 채취한 종자를 2017년 파종한 뒤 우수개체로 선발되어 2017년부터 2020년까지 4년간 촉성, 억제, 자연개 화 작형별 주년생산성 검정을 수행하여 선발된 우수계통을 ‘SP17-425-01’이라 계통명을 부여하였다. 2020년 최종적으 로 우수한 계통으로써 평가 및 심의를 받은 뒤 ‘충남SP-68 호’로 명명하였고, 당해 년도 12월 직무육성 신품종 선정위원 회를 통과하여 품종명은 ‘Pinky Lang’으로 하였다. ‘Pinky Lang’은 고온기에 화색이 변하지 않고 녹심이 매우 진하며 줄 기가 튼튼한 품종이다. 여름철 흰녹병에도 강하여 고온기 수출 품종으로 적합하다고 판단되었다. ‘Pinky Lang’은 연중 3 작기 재배테스트에서 단일처리 후 개화소요일수는 자연개화 작형 52일, 촉성작형 49일, 억제작형 50일이었고, ‘Yes Luna’ 는 각각 51일, 52일, 52일로 전반적으로 개화소요일수가 짧 았다. ‘Pinky Lang’의 절화장과 줄기굵기는 각각 104.5cm, 4.71mm으로 ‘Yes Luna’가 109.5cm, 4.25mm인 것에 비 해 5cm정도 짧았고 줄기굵기는 0.46mm 더 굵었다. ‘Pinky Lang’의 꽃 크기는 5.6cm이고 ‘Yes Luna’는 6.8cm로 1.2cm 작았다. ‘Pinky Lang’의 한 줄기에 달리는 꽃의 수와 한 송이 당 꽃잎수는 각각 27.7개, 30.9매였고, ‘Yes Luna’ 는 각각 18.0개, 37.0매인 것으로 착화수는 9.7개 많았고 꽃 잎수는 6.1매 적었다. 여름철 재배테스트를 통해 확인해본 결 과 흰녹병 저항성이 높은 경향을 확인하지만, 1~10% 정도의 병반을 보여 완전저항성을 가지진 못하는 것으로 사료된다. 자연개화 작형에서 절화수명의 경우는 24.5일로 ‘Yes Luna’ 에 비해 2.1일 길었다. ‘Pinky Lang’은 과습한 경우 줄기가 연약해질 수 있기 때문에 생장억제제인 Daminozide를 처리 하여 줄기를 강건하게 하고 절화 품질의 균일도를 높여 고온 기 재배에 안정적인 생산이 가능하여 수출할 수 있다.

Background: Water deer and sika deer, which breed in the wild environment, are known to have similar reproductive physiology mechanisms. Therefore, this study aimed to analyze the differences in uterine development between water deer and sika deer during estrus. Methods: MMPs and uterine development-related factors were analyzed and morphological differences were compared in the uterus of sika deer captured near Russia near Korea and water deer captured in the wild in Korea. Results: In terms of morphological differences in the uterus, the glands that form villus within the endometrium of the water deer were newly developed, and the formation of small glands was high, but the villus and glands of the sika deer were expanded, and the stroma zone in the myometrium was higher than that of the water deer. Development has increased. Additionally, the expression of PAPP-A and VEGF factors was increased in the endometrium of water deer than in sika deer, but the actions of MMPs were increased in sika deer. Conclusions: As a result of this study, there is a significant difference in the development of glands in the endometrium of water deer and sika deer during estrus, and it is believed that there is a significant difference in the development of the uterus due to the physiological effects of estrus between water deer and sika deer. Additionally, it is believed that there will be differences in the timing at which pregnancy can be decided.

Nasopharyngeal stenosis is defined as a morphological transition of narrowing at the nasopharyngeal region. A 2-yearold, castrated male, Korean short hair cat was referred to the animal medical center, Gyeongsang National University. According to clinical signs, diagnostic imaging, and physical examination, nasopharyngeal stenosis was diagnosed. The staphylectomy was performed using a CO2 laser, and there were not any post-operative complications. The patient was discharged in two days. This report describes the case of nasopharyngeal stenosis in cat and represents that laser ablation could be a good option for surgical management of the nasopharyngeal region with a low complication rate.

본 연구는 국내에서 실내 관상용으로 많이 활용되는 스킨답 서스의 실내재배 시 적정 인공광원, 수경재배 전기전도도 농 도 및 용토를 선발하기 위하여 수행하였다. 실내재배 시 인공 광원은 형광등, 적청 LED, 백색 LED로 처리하였다. 수경재배 시 양액 농도는 EC 1.2, 1.6, 2.0ds·m-1 수준으로 하였고, 재 배용토는 제올라이트, 코코피트, 펄라이트, 황토볼 4가지로 달리하였다. 스킨답서스의 실내 재배 시 줄기 길이는 형광등 과 적청 LED 처리보다 백색 LED 처리에서 길어졌다. 잎의 크기는 적청 LED 처리에서 형광등과 백색 LED 처리보다 커 지는 경향이었다. 엽록소 지수값은 백색 LED>적청 LED>형광 등 순으로 높았다. 수경재배 시 스킨답서스의 줄기길이는 EC 농도가 높아질수록 길어지는 경향이었고, 용토별로 비교하면 제올라이트와 코코피트에서 높은 경향이었다. 엽록소 지수값 도 EC 농도와 비례하여 높아졌고, 용토별로는 제올라이트와 코코피트에서 높았다. 스킨답서스의 생체중도 EC 농도가 높 을수록 높았고, 제올라이트와 코코피트에 높은 경향이었다. 상기 결과들을 종합할 때, 스킨답서스의 NFT를 이용한 실내 재배 시 적정 인공광원으로는 백색 LED를, 수경재배 양액의 농도는 EC 1.6∼2.0ds·m-1를, 그리고 NFT 용토는 제올라이 트나 코코피트가 적합하다는 것을 알 수 있었다.

Currently, the number of animals administered in animal hospitals and drugs prescriptions has increased. At the same time, the number of cases of violation of administrative measures due to violations of the Narcotics Control Act has also increased. Therefore, it is necessary to review whether the current status of drug prescriptions, management and supervision systems in animal hospitals are correct and whether there is anything to be supplemented. Although drug handling is managed through NIMS, there are problems such as poor supervision due to the NIMS single system, reporting exceptions that form blind spots for management supervision, and lack of connection between prescriptions and medical departments within the system. In order to overcome these problems, the following improvement plans are proposed. First, reduces the hassle of handling reports and facilitates verification of medical records and prescriptions by strengthening the link between NIMS and animal hospital electronic charts, and strengthens the link between drug handling reports of medical institutions and drug handling reports of animal hospitals to prevent exposure to medical shopping. Second, by introducing a preliminary notification system into the veterinary system, drug misuse is prevented through prior notification in accordance with the criteria for preventing misuse of animal drugs. Third, by modifying the NIMS reporting system by introducing disease classification symbols, the exact cause of the increase in use is identified by preparing drug use data for each disease. Based on these improvements, it is expected that a management system that reflects the actual medical environment of animal hospitals will be established and drug abuse will be prevented based on guidelines.

Background: Canine induced pluripotent stem cells (iPSCs) are an attractive source for veterinary regenerative medicine, disease modeling, and drug development. Here we used vitamin C (Vc) to improve the reprogramming efficiency of canine iPSCs, and its functions in the reprogramming process were elucidated. Methods: Retroviral transduction of Oct4, Sox2, Klf4, c-Myc (OSKM), and GFP was employed to induce reprogramming in canine fetal fibroblasts. Following transduction, the culture medium was subsequently replaced with ESC medium containing Vc to determine the effect on reprogramming activity. Results: The number of AP-positive iPSC colonies dramatically increased in culture conditions supplemented with Vc. Vc enhanced the efficacy of retrovirus transduction, which appears to be correlated with enhanced cell proliferation capacity. To confirm the characteristics of the Vc-treated iPSCs, the cells were cultured to passage 5, and pluripotency markers including Oct4, Sox2, Nanog, and Tra-1-60 were observed by immunocytochemistry. The expression of endogenous pluripotent genes (Oct4, Nanog, Rex1, and telomerase) were also verified by PCR. The complete silencing of exogenously transduced human OSKM factors was observed exclusively in canine iPSCs treated with Vc. Canine iPSCs treated with Vc are capable of forming embryoid bodies in vitro and have spontaneously differentiated into three germ layers. Conclusions: Our findings emphasize a straightforward method for enhancing the efficiency of canine iPSC generation and provide insight into the Vc effect on the reprogramming process.

Canine hyperadrenocorticism, a prevalent endocrine disorder characterized by excessive cortisol production. Notably, hypercoagulability leading to pulmonary thromboembolism (PTE) poses a substantial concern. PTE may be underestimated because of the rapid dissolution of canine thrombi postmortem. However, traditional coagulation assays face challenges in early detection of hypercoagulability. Therefore, this study explored the use of thromboelastography (TEG) as a diagnostic tool for identifying hypercoagulability in dogs with hyperadrenocorticism. A total of 31 dogs visited the Gyeongsang Animal Medical Center between 2018 and 2022, comprising 21 dogs with hyperadrenocorticism and 10 controls who underwent clinical and coagulation analyses. Hyperadrenocorticism was diagnosed using a low-dose dexamethasone stimulation test or adrenocorticotropin hormone stimulation test, and conventional laboratory parameters and coagulation parameters, such as the prothrombin time, activated partial thromboplastic time, fibrinogen, and TEG results, were compared between the groups. Clinical data revealed significantly elevated monocyte, platelet, alanine aminotransferase, alkaline phosphatase, triglyceride, and cholesterol concentrations in dogs with hyperadrenocorticism, which were attributed to excess cortisol secretion (p<0.05). TEG analysis demonstrated significantly decreased K values and increased α and MA values in hyperadrenocorticism dogs (p<0.05), indicating a shortened clotting time and enhanced clot strength, suggestive of hypercoagulability. TEG effectively highlights hypercoagulability in dogs with hyperadrenocorticism and provides valuable insights in predicting blood clot formation. Although predicting clot formation in dogs remains complex owing to multifactorial influences, this study underscores the potential utility of TEG in enhancing such predictions for dogs with hyperadrenocorticism.

The subject of this paper is the language usage within legal discourse, with the corpus sourced from the legislation of the People’s Republic of China. Zhang Deming (1989) believes that “register is a scientific term in the category of language stylistics, a ‘functional style’ of language formed due to different communicative functions of language use. This system has a series of commonalities in linguistic materials (elements) and rhetorical methods, and there are a series of differences and corresponding patterns between different systems.” According to his view, the legal register has distinctive features which differ from other registers. This paper describes and interprets the syntactic, lexical, and register aspects of legal discourse, along with the linguistic style inherent in legal language.