The purpose of this study was to investigate the effect of rope skipping and band exercise on GH, IGF-1 and metabolic syndrome risk factors in female obese middle school students. Twenty female obese middle school students(%BF > 30%) were randomly assigned to a exercise group(EX, n=10) or control group(CON, n=10). The exercise group has performed rope skipping and band exercise for 12 weeks, 3time per week. Exercise intensity was increased gradually, from 40% to 70% of Heart Rate Reserve(HRR) and Rating of Perceived Exertion(RPE) 11-15. GH, IGF-1 and metabolic syndrome rist factors were GH, IGF-1 and metabolic syndrome risk factors were measured pre- and post-exercise program including rope skipping and band exercise. Levels of GH and IGF-1 were significantly increased (p<.01 and p<0.001, respectively) in exercise group after 12 weeks of exercise training. WC and TG were significantly decreased(p<.01) in exercise group after 12 weeks of training. HDL-C was significantly increased(p<.05) in exercise group after 12 weeks of training. In conclusion, rope skipping and band exercise were effective exercise program in improving GH, IGF-1 and metabolic syndrome risk factors in female obese middle school students.

A xylan-decomposing Gram-positive bacterium, Cellulosimicrobium cellulans DY-8, was isolated from the gut of a wood-feeding longicorn beetle, Moechotypa diphysis. To amplify a partial fragment of the GH 10 β-1,4- xylanase (XylC) gene of strain DY-8, two degenerated oligonucleotide primers were designed based on strictly conserved regions (WDVVNE and ITELLDV) in the GH family 10 xylanolytic enzymes. The full gene (1488-bp) of XylC, which was predicted to encode a protein consisting of 495 amino acids with a molecular mass of 52.0 kDa and a calculated pI of 6.49, was cloned by repeated DNA walking and nested PCR protocols. The results of a protein blast survey showed that XylC was a β -1,4-xylanase comprised of an N-terminal catalytic GH10 domain (from Ser48 to Leu338) and a C-terminal RICIN domain (from Tyr359 to Leu492). This overall structure of XylC was 57% identical to that of Actinoplanes sp. SE50/110 β -1,4-xylanase (Accession number: YP_006265966), which has not yet been biochemically characterized.

Growth hormone (GH) is obligatory for growth and development. But, there is controversy on the GH effect about reproductive processes of sexual differentiation, pubertal maturation, gonadal steroidogenesis, gametogenesis and ovulation. This study was conducted to investigate the effect of GH on estrus, ovulation and embryo implantation. The results obtained were as follows. GH stimulated to increase estrus rate (p<0.05), pregnancy rate (p<0.05), and total fetus number in mice treated for superovulation. Also, the correlation between GH and steroids, E2 and P4, at peri-estrus stage/ peri-ovulation stage/ peri-implantation stage of the superovulation-induced mice was examined. Consequently, GH co-injected with PMSG especially increased P4 level (p<0.05) at peri-estrus stage of superovulationinduced mice. In conclusion, GH co-treatment in superovulation system boosted the rate of estrus, pregnancy and total fetus by increasing progesterone level at peri-estrus stage of superovulation-induced mice.

The extracellular GH11 β-1,4-xylanase (XylY) gene (633-bp) of Paenibacillus sp. strain KYJ-16 was molecularly cloned by repeated DNA walking and nested PCR method. The xylY gene was predicted to encode an extracellular protein consisting of 611 amino acids with a nesuced molecular mass of 23 kDa and a calculated pI of 9.55. Protein blast search revealed that the enzyme consisted of a putative catalytic domain, which is homologous to a catalytic GH11 domain. The highest sequence identity (92%) was obtained as the catalytic GH11 domain of XylY was compared to that of Paenibacillus sp. strain HGF5 (GenBank accession number: EGG35584) that has not yet been characterized. Enzymatic properties of the recombinant His-tagged enzyme (rXylY) overexpressed in E. coli BL21 harboring pET-28a(+)/xylY will be also presented.

본 연구에서는 돼지의 체지방을 감소시키고 성장을 촉진시키는 인자인 PGH를 cloning하여 이 유전자를 외래 유전자의 발현이 유도적으로 조절되는 Tet system에 도입하고자 하였다. 또한 유전자의 발현이 turn on되었을 때 그 발현 정도를 최대화하기 위하여 WPRE 서열을 도입하였다. 구축된 각각의 vector는 retrovirus 생산 세포주에 도입하여 virus를 생산하였으며 이를 여러 종류의 표적세포에 감염시켜서 PGH 유전자의 발현을 확인한 결과, 1×10/sup 6/ 세포에서 350∼2,100 ng의 PGH가 분비되었으며 특히 PFF 세포에서 가장 높은 발현을 나타내었다. Tet system에 도입된 PGH의 발현이 유도적으로 조절되는지를 PFF 세포에서 확인한 결과, 유도 효율이 2∼6배로 나타났으며 WPRE 서열이 rtTA 유전자의 downstream에 위치한 조건에서 가장 높은 유도 효율을 나타내었다. 이러한 PGH 유전자의 유도적인 발현의 조절은 고급육 생산의 형질전환 돼지 연구에 있어서 가장 큰 문제점이 되는 PGH 유전자의 과다한 발현에 의한 생리적인 부작용을 최소화할 수 있는 해결 방안으로 제시될 수 있을 것이다.

Bacillus genus are found abundantly in various sites and their secondary metabolites were used as potential agents in agriculture, notably plant growth promoting and bio-control. The objective of this study was to develop the culture conditions of GH1-13 strain including higher cell growth, stable endospore-forming and enhancement of potential agents which are related with plant growth promoting and phytopathogen suppression. The optimal carbon and nitrogen sources were determined by glucose and soy bean flour, respectively, then resulted in 7.5×109 cells/mL, 6.8×109 endospore cells/mL and sporulation yield of 90% after 30 h cultivation in 500 L submerged fermenter at 37℃, pH 7.0. Cells and cell-free supernatant of GH1-13 strains showed the potent antifungal activity against phytopathogenic fungi of Colletotrichum gloeosporioides. It was also confirmed that indole-3-acetic acid (IAA) production of GH1-13 strain was greatly increased by addition of 0.3% tryptophan.

Using Agrobacterium-me야ated transformation method the auxin-regulated cotton GST (Gh-5) constructs were used to transform Rehmannia glutinosa L. The PCR analysis was conducted to verify transgenicity. Based on the PCR analysis, there was verified that the 988 bp DNA band had showed in transgenic plant genomes in PCR anaJysis using Gh5-1 and Gh5-2 primers. The effects of cocultivation with Agrobacterium tumefaciens, regeneration and selection conditions on the transformation efficiency of Chinese foxglove (Rehmannia glutinosa L.) were investigated. Factors such as cocultivation period, use of acetosyringone, postcultivation in darkness, and different kanamycin concentrations for selection were assessed. In vitro regeneration, the number of leaves, shoot lengths and numbers on MS medium were superior to on B5 and WPM medium, and the shoot formation rate was highest level of 95% in cultured base part containing leaf stalk. Addition of acetosyringone at concentration of 200μM to cocultivation medium and 3-day of cocultivation improved transformation frequencies. Exposure of explants to darkness for 4 weeks on selection medium resulted in further increased the regeneration frequency of transgenic shoots. In PCR analysis, the amplified fragments of Gh5 gene were detected (988 bp), and GST-expressing transgenic R. glutinosa L. plants had approximately three-fold higher activity in leaf extracts compared with control plant.

월동기 90일 동안 transgenic coho salmon의 사료섭식량, 성장도 및 혈중 호르몬 수준을 파악하였다. 실험 종료시 실험구별 사료섭식량은 비교해 볼 때 tGH구가 Wild구보다 약4배 정도 높게 나타났지만, 사료효율의 경우 오히려 tGH구가 Wild구보다 1.1 배 낮은 것을 알 수 있었다. 한편 생존률은 tGH구가 Wild구보다 높게 나타났고, 성장은 tGH구가 길이에 있어 1.4배, 무게의 경우 약 3배 빠르게 나타났으며, 비만도 역시

수도에 있어서gl(smooth 잎), gh(gold hull) 몇 wx(찰)인자의 표식인자로서의 이용을 목적으로 glgl ghgh wxwx의 특성을 가친 Javanica품종 Malagkit sinaguing을 일회친으로 하고 GLGL GHGH WxWx의 특성을 가진 단간 다수성 계통 IR 667-98-1-2-2와 IR 1317-266-2-3을 반복친으로 하여 연속 2회 여교잡하고 그 F3 세대를 다시 반복친에 교배함으로서 동일한 유전적 배경을 가진 8개의 isogenic line(인자형)을 육성하였으며 이들 isogenic line을 이용하여 g1, gh 및 wx인자의 수량성 및 수량구성형질에 미치는 영향을 검토하였던 바 그 결과를 요약하면 다음과 같다. 1. 2회의 연속 여교잡에 의하여 비대립적인 간장형질이나 gl, gh 및 wx인자등 열성형질을 비교적 소집단에 의하여 효율적으로 반복친에 이전할 수 있었다. 2. 유전적 배경이 동일한 glgl ghgh wxwx계통과 GLGL GHGH WxWx계통의 F2 로부터 glgl wxwx, glgl ghgh WxWx, glgl GH-wxwx, glgl GH-WxWx, GL-ghgh wxwx, GL-ghgh WxWx, GL-GH-wxwx 및 GL-GH-WxWx등 8개의 인자형에 해당되는 8개의 near isogenic line을 육성할 수 있었다. 3. gl, gh 및 wx인자는 단순열성으로 분리되었으며 본조합에서의 조생은 만생에 단순우성이었고 이들 인자들은 상호 독립적이었다. 4. gl인자형을 출수기와 gh인자형은 임실율과 그리고 wx인자형은 수수 및 1,000립중과 상관이 높은것으로 나타났다. 5. 각 isogenic line(인자형)의 수량성간에 유의한 차이가 있었으며 황금색영과 짚색영영, 찰과 메간에 수량성의 유의한 차이가 인정되었다. 즉 짚신색영과 메는 그들 상대형질계통에서 보다 수량이 더 높았다. 6. 인자형간의 상호작용은 출수기에서는 유의하였으나 출수기를 제외한 타형질들에서는 유의하지 못하였다. 7. 2개 열성인자들이 조합된 계통들은 그에 대립되는 2개 우성인자들이 조합된 계통들에 비하여 간장과 립수를 제외한 모든 형질들에서 평균치는 낮고 변이계수는 더 크게 나타났다. 8. 3개 열성인자가 조합된 glgl ghgh wxwx계통은 그에 대응되는 GL-GH-WxWx계통에 비하여 모든 형질에서 평균치는 낮고 변이계수는 더 크게 나타났다. 9. 각 isogenic line에서 수량과 가장 상관이 높았던 형질은 입수로 나타났으며 isogenic line에 따라서 는 출수기, 간장 및 수장등이 수량과 유의한 정의 상관을 보였다. 10. 각 isogenic line에서 형질간의 상관은 isogenic line에 따라 달라서 glgl wxwx계통들에서는 수수와 입수간에, ghgh WxWx계통들에서는 수수와 입수간 및 간장과 입수간에 정의 상관이, ghgh wxwx계통들에서는 입수와 임실율간에, GL-GH-계통들에서는 출수기와 임실율간에 부의 상관이 유의하였다. 11. 경로 분석결과 수량에 대한 직접효과는 입수에서 가장 컸고 임실율, 1,000립중의 순이었으며 수수는 입수를 통한 간접효과가 컸으며 입수는 임실율에 감소방향으로 작용하였다. 12. gl, gh 및 wx인자를 표식인자로서 도입하는 경우 이들 특성을 지닌 수량성에 지장이 없는 계통을 선발할 수 있는 가능성을 지적하였고 각 인자에 대한 효율적인 선발에 관하여 고찰하였다.