계란의 비린내 강도는 가금류의 종류와 개인의 인식에 따라 다르지만 여러 요인에 의해 발생할 수 있다. 특히 트 리메틸아민(TMA)이 원인으로 밝혀졌다. 주목할만한 점은 리보플라빈이 TMA를 무취의 트리메틸아민-N-옥사이드로 전환시키는 역할을 하는 효소인 플라빈 함유 모노옥시게 나제 3의 활성을 증가시킬 수 있다는 점이다. 본 연구는 난황의 TMA 함량을 분석하여 비린내에 대한 기여도를 평 가하고, 비린내를 방지하는 방법을 개발하는 것을 목적으 로 하였다. 고체상 미세추출-기체 크로마토그래피/질량 분 석법을 사용하여 리보플라빈이 강화, 보충된 사료를 먹인 암탉의 난황에 있는 휘발성 화합물을 감지하고 정량화했다. 또한 샘플 간 휘발성 물질의 상대적 함량을 비교하기 위해 전자코를 이용하여 상관관계 연구를 수행하였다. TMA는 콜린이 함유된사료를 섭취한 가금류의 난황에서 고농도로 검출되었지만 리보플라빈이 보충된 사료를 섭취한 가금류 에서는 검출되지 않았다. 전반적으로, 이 연구는 리보플라 빈이 TMA를 포함하여 계란에 존재하는 휘발성 물질의 양 과 품질에 영향을 미친다는 것을 시사한다. 이러한 발견 이 계란의 비린내를 줄이는 것은 물론 품질 향상에 기여 하기를 기대한다.

The results of internal temperature. productivity and immunity analysis of the laying hen house by the thermal environment and the supply of cold drinking water were as follows. The external temperature was changed from the minimum of 18℃ at night and the maximum of 36℃ during the day, and the internal temperature of the laying hens varied from 20~31℃. Thermal imaging analysis showed that the body temperature of the laying hen decreased by 2.4℃ with the supply of drinking water. The laying hen amount increased 2.36g and laying hen rate increased 3.62%p. Albumin increased 6.1%, decreased AST 15%, and decreased cholesterol 12.7%. Immune activators increased and T cells and B cells increased to increase immunity.

본 연구는 케이지와 평사 사육환경이 산란종계의 생산성에 미치는 영향을 알아보고자 실시하였다. 케 이지 또는 평사 사육환경을 제외한 모든 환경 조건은 동일한 산란종계 무창 계사를 선정하여 진행하였 다. 총 48주간 산란 종계의 산란율, 폐사율, 수정율 및 부화율을 측정하였다. 산란초기 산란율은 케이 지 사육 환경에서 높았으나, 전 구간을 보았을 때 37주 이후부터는 평사 사육이 월등히 높게 나타났다. 폐사율은 암컷 종계의 누적 폐사율에는 유의한 차이가 없었지만, 수컷의 폐사율은 케이지 사육환경에서 유의적으로 높게 조사되었다. 수정율은 전 구간 평사 사육이 월등히 높게 나타났다. 부화율에서도 평사 사육이 케이지 사육보다 높게 나타났다. 본 연구결과 평사 사육방식이 케이지 사육방법보다 산란종계의 수정율, 부화율 및 폐사율에서 우수한 것으로 조사되었다.

The drinking water supply system applicable to the laying hen consists of air-water heat pumps, drinking water tanks, heat stroage tank, circulation pumps, PE pipes, nipples, and control panels. When the heat pump system has power of 7.7 to 8.7 kW per hour, the performance coefficient is between 3.1 and 3.5. The supply temperature from the heat pump to the heat stroage tank was stabilized at about 12±1°C, but the return temperature showed a variation of from 8 to 14°C. Stratified temperature in the storage tank appeared at 12.°C, 13.5°C and 14.4°C, respectively. The drinking water supply temperature remained set at 15°C and 25°C, and the conventional tap water showed a variation for 23°C to 30°C. As chickens grow older, the amount of food intake and drinking water increased. y = -0.0563x2 + 4.7383x + 8.743, R2 = 0.98 and the feed intake showed y = -0.1013x2 + 8.5611x. In the future, further studies will need to figure out the cooling effect on heat stress of livestock.

Ovarian cancer is the most lethal world-wide gynecological disease among women due to the lack of molecular biomarkers to diagnose the disease at an early stage. In addition, there are few well established relevant animal models for research on human ovarian cancer. For instance, rodent models have been established through highly specialized genetic manipulations, but they are not an excellent model for human ovarian cancer because histological features are not comparable to those of women, mice have a low incidence of tumorigenesis, and they experience a protracted period of tumor development. However, the laying hen is a unique and highly relevant animal model for research on human ovarian cancer because they spontaneously develop epithelial cell-derived ovarian cancer (EOC) as occurs in women. Our research group has identified common histological and physiological aspects of ovarian tumors from women and laying hens, and we have provided evidence for several potential biomarkers to detect, monitor and target for treatment of human ovarian cancers based on the use of both genetic and epigenetic factors. Therefore, this review focuses on ovarian cancer of laying hens and relevant regulatory mechanisms, based on genetic and epigenetic aspects of the disease in order to provide new information and to highlight the advantages of the laying hen model for research in ovarian carcinogenesis.

To elucidate the effect of feeding differently prepared CaCO3 (industrially purified limestone) on laying performance, egg shell quality and serum calcium and phosphorus concentration, totally 288 layers (52 wks old), with 16 birds per replicate, 3 replicates per treatment for six treatments were employed into the series of CaCO3 treatments followed by feeding study. Six CaCO3 treatment and diet preparation includes mash diet with naturally sized CaCO3 (MNaC), mash diet with coarsely particled CaCO3 (MCoC), mash diet with finely particled CaCO3 (MFiC), pelleted diet with naturally sized CaCO3 (PNaC), pelleted diet with casein coated CaCO3 (PCnC) and pelleted diet with agar coated CaCO3 (PAgC). Hen day egg production of the bird fed both fine CaCO3 and pelleted CaCO3 were lower (p<0.05) than those fed other CaCO3 diets. Although there is no difference in average egg weight among treatments, the weight tended to be heavier once the egg production rate was low. There is no difference in feed conversion ratio (intake/egg) among treatments. Egg shell thickness was the highest (p<0.05) with feeding of MCoC whereas the value was the lowest with feeding of pelleted CaCO3 diet. Although there is some difference in egg shell thickness, the shell breaking forces were not significantly different among treatments. Calcium content of the shell was the lowest (p<0.05) in the egg from the layer fed finely particled CaCO3. Serum Ca content tended to decrease up to 8 hrs after feeding, then tended to increase afterwards. Although both agar and casein coating of CaCO3 tended to retard the recovering increase of blood Ca, the difference needed to be clarified with individual ovulation cycle. Serum P level was generally decreased as time passed after feeding. Crude protein utilizability was the highest with feeding of agar coated CaCO3 and that of fat was higher with hens fed pelleted diets. Both ash and P utilizabilities of diet with finely particled CaCO3 were the highest among treatment although there were no differences in both energy and Ca utilizabilities. Conclusively, both the particle size of CaCO3 and pelleting of CaCO3 diet could affect shell quality of the egg. The effect was more evident by the differentiation of CaCO3 particle size than any other processing such as pelleting and coating.