This study aimed to estimate the accumulated temperature requirements for phenological changes in Lilium. Eight cultivars of three lily types were cultivated in open field conditions for phenological observations based on floral organ development. Growing degree days (GDD) requirements for phenological changes were calculated and verified using Lilium LA hybrid ‘Serrada’ under greenhouse conditions. Lilium Oriental hybrids exhibited higher GDD requirements compared to Lilium FA and LA hybrids for their phenological development. Estimations of phenological change dates in greenhouse cultivation were accurate within 1–3 days. These results provide a reliable description for predicting lily development stages across diverse cultivation environments by quantifying the accumulated temperature requirements for key phenological events.

Cucumber mosaic virus (CMV) poses a considerable threat to a diverse array of crops in global agriculture. CMV impacts commercially important cut lilies by diminishing both yield and flower quality. We used RNA sequencing (RNA-seq) to investigate the changes in gene expression in the leaves and bulbs of four distinct cultivars of cut lily, ‘Cancun,’ ‘Brunello,’ ‘Connecticut King,’ and ‘Casa Blanca’ following CMV infection. Notably, CMV affected photosynthetic processes by significantly downregulating genes associated with photosynthesis. In addition, CMV infection was detrimental to chloroplast function and energy production. We observed differential expression of genes associated with both dominant and recessive resistance pathways that are crucial for preventing virus entry, replication, and systemic spread within the plant. Based on functional annotation and differential gene expression analysis, we identified the regulatory genes involved in triggering immune responses, modulating signal transduction, and specific host factors during CMV infection. To validate the RNA-seq findings, we selected four genes involved in resistance, virus multiplication, and virus spread and analyzed them using real-time quantitative reverse transcription PCR (qRT-PCR) with specific primers. The qRT-PCR results aligned closely with those from RNA-seq, showing consistent fold-change responses for the genes that were differentially expressed, indicating that the RNA-seq results were reliable. These results deepen our understanding of the complex genetics behind plant-virus interactions while also providing information for breeding programs that aim to develop CMV-resistant lily cultivars.

This study investigated the growth dynamics of Lilium miquelianum bulb scales cultured in four different media formulations: Full Murashige-Skoog (FMS), Half Murashige-Skoog (HMS), Anderson Vitamin (AV), and Knudson Vitamin (KV). Bulb scales were collected from native populations on Jeju Island, Korea, and cultured in vitro for 12 weeks. Growth parameters, including bulb area and scale count, were measured at 3-week intervals. Statistical analyses revealed significant differences in growth rates across media types and time points. FMS consistently demonstrated the highest growth rates and final bulb area and scale count, while KV exhibited the lowest performance. Heatmap analysis showed that FMS achieved the highest weekly growth rates in most time points, with HMS showing comparable performance during early growth stages. Individual sample growth rates varied within media, highlighting the influence of both external and internal factors on growth. Correlation analysis revealed a positive association between bulb size and scale quantity, with FMS exhibiting the strongest relationship. These findings emphasize the importance of appropriate media selection for optimal L. miquelianum propagation, with FMS being the most suitable for extended cultivation. This research provides valuable insights for improving propagation efficiency and conservation efforts of L. miquelianum.

Mathematically modeling photosynthesis helps to interpret gas exchange in a plant and estimate the photosynthetic rate as affected by environmental factors. Notably, the photosynthetic rate varies among leaf vertical positions within a single plant. The objective of this study was to measure the distinct photosynthetic rate of lily (Lilium Oriental Hybrid ‘Casa Blanca’) at the upper, medium, and basal leaf positions. Subsequently, the FvCB (Farquhar-von Caemmerer-Berry) photosynthesis model was employed to determine the parameters of the model and compared it with a rectangular hyperbola photosynthesis model. The photosynthetic rates were measured at different intracellular CO2 concentrations () and photosynthetic photon flux density (PPFD) levels. SPAD values significantly decreased with lowered leaf position. The photosynthetic rates at the medium and basal leaves were lower compared with the upper leaves. FvCB model parameters,  and   , showed no significant difference between the medium and basal leaves. Estimated photosynthetic rates from derived parameters by the FvCB model demonstrated over 0.86 of R2 compared with measured data. The rectangular hyperbola model tended to overestimate or underestimate photosynthetic rates at high  with high PPFD levels or low  with high PPFD levels, respectively, at each leaf position. These results indicated that the parameters of the FvCB model with different leaf positions can be used to estimate the photosynthetic rate of lily.

Salinity stress is a major threat to plant growth and development, affecting crop yield and quality. This study investigated the effects of different salinity levels on photosynthetic responses and bulb growth of Lilium LA hybrid “‘Serrada’.” Plants were irrigated with 1 L of 0, 200, and 400 mM NaCl solutions every two weeks for 14 weeks in a greenhouse. At the end of the cultivation period, the substrate pH decreased, and electrical conductivity increased with increasing salinity. Regardless of salinity levels, the days to flowering and number of flowers were similar among treatments. In contrast, the flower width, plant height, number of leaves, and leaf area decreased with increasing NaCl concentrations. Although there were no differences in the photosystem II (PSII) operating efficiency and maximum quantum yield of PSII, net CO2 assimilation rates (An) and stomatal conductance (gs) were significantly reduced at 200 and 400 mM NaCl solutions compared to the control. At 400 mM NaCl solution, bulb diameter and weight significantly decreased at the end of the experiment. These results suggest that bulb growth inhibition could be attributed to limiting photosynthetic rate and stem growth. This finding suggests that salinity mitigation is necessary to maintain plant growth and photosynthetic capacity in lily cultivation on salt-affected soils.

Attempts to increase the usability of lilies led us to prepare pulverized lily bulbs, which we then added to bread flour to bake white pan bread. The properties of the frozen dough and the quality characteristics of the bread were analyzed. Our experiments showed that the addition of lily powder decreases the viscoelasticity and stability of frozen dough. The absorption rate of the frozen dough was 63.1±0.2% for the control sample, and 66.1±0.1% and 70.9±0.2% for the normally pulverized samples containing 3% and 5% of lily powder, respectively, whereas the absorption rate of the finely pulverized samples tended to increase slightly. The gelatinization results of the frozen dough decreased with the addition of lily powder in terms of the final viscosity, break down, and setback values. Compared to the control sample, the specific volume of the bread products that underwent normal pulverization (5% additive) increased slightly and decreased for the lily bulbs that were finely pulverized (3% additive). The addition of lily powder did not significantly affect the water activity during the storage period of up to 5 days. The hardness of white pan bread increased from 1,948±114.3 to 2,518±154.7 g/㎠ on the first day of storage to 2,571±160.9 to 3,265±147.4 g/㎠ on the 5th day of storage. The hardness was the highest for the 5% sample that underwent normal pulverization, and the samples differed significantly. The longer the storage period, the lower the springness value of the white pan bread became, and this result was most notable for the finely pulverized powder sample. The springness of white pan bread decreased as the amount of lily powder additive and the storage period increased. The sensory test results were excellent in that the appearance, texture, flavor, taste, and overall preference for white pan bread to which 3% of normally crushed lily powder had been added had improved relative to the control sample.

정원용 아시아틱나리 유전자원 ‘Tiny Dino’, ‘Elite’, ‘Orange Pixie’와 ‘Partner’를 2000년에 수집하여 2014년까지 ‘Tiny Dino’ 와 ‘Orange Pixie’의 교배조합에서 선발한 모계(L2-3-4)와 ‘Elite’ 와 ‘Partner’의 교배조합에서 선발한 부계(L2-9-6)를 6차례 자가 수분(S6)하여 선발하였다. 이들을 2014년에 교잡하여 채종한 것에서 유래된 구근을 노지재배 하였고, 이것의 생육 및 개화 특성을 바탕으로 2016년에 아시아틱나리 ‘Orange LiA’를 선발하였다. 정원용 아시아틱나리 일대잡종 ‘Orange LiA’은 주황색의 사발모양의 홑꽃으로 반점이 적고 반점의 색은 자주갈색이다. 식물체 당 개화수가 4.2개로 많은 편이며, 화경이 15.1cm로 중간 정도이고 꽃차례 형태는 총상꽃차례로 개화 방향은 상향이다. 초장은 30.5cm이고, 잎의 수는 23.4개이고, 잎의 길이는 8.1cm이며, 잎의 폭은 1.8cm이다. 일대 잡종 ‘Orange LiA’은 대조품종인 ‘Tiny Dino’ 보다 초장이 짧고 일찍 개화되며 월동이 가능하므로 정원용으로 선호도가 우수하다. 또한 이 품종은 양친을 순계화하여 육성하였기 때문에 품종의 재현이 가능하다.

한국 땅나리와 섬말나리의 수요는 최근까지도 늘어났지만 종자 상태에서 개화에 이르기까지 확률은 낮은 편으로 자생지에서의 개체수는 해마다 감소하였다. 본 연구는 땅나리(Lilium callosum Siebold & Zucc.)와 섬말나리(L. hansonii Leichtlin ex D.D.T. Moore)의 대량증식을 도모하기 위하여 종자 발아에 유리한 주·야간 온도 조건과 적합한 GA3 농도를 알아보는 것을 목적으로 수행되었다. 땅나리 종자는 2017년 10월 24일 강원도 춘천히 둔산면 원창리(37°47’10.00”N, 127°44’31.00”E), 섬말나리 종자는 2017년 10월 2일 울릉도 울릉군 북면 나리 (37°31’27.06”N, 130°52’25.08”E)에서 채종하였다. 땅나리와 섬 말나리 종자들을 주·야간 15/5, 20/10, 25/15, 20/20°C의 환경 조절상에서 처리구 당 30립씩 3반복으로 치상하여 온도 처리 실험을 진행한 후, 발아율 결과가 가장 우세했던 온도 조건의 환경조절상에서 섬말나리 GA3 처리 실험을 추가로 진행하였다. 온도 처리 실험결과 땅나리는 20/20°C의 온도 조건에서 발아율이 95.0%로 가장 높았으며 평균발아소요일수 또한 10.1일로 가장 낮았다. 섬말나리는 20/20°C의 온도 조건에서 발아율이 78.8%로 가장 높았으며 평균발아소요일수 또한 40.1일로 가장 낮았다. 이를 토대로 20/20°C에서 실시한 섬말나리 GA3 처리 실험은 증류수를 대조구로하여 200, 400, 800mg･L-1의 농도로 상온에서 24시간 침지한 후 처리구 당 20립씩 3반복으로 치상하였다. 그 결과 대조구에서 81.6%로 가장 높은 발아율을 나타냈으며 평균발아소요일수는 200mg･L-1처리구에서 37.1일로 가장 낮았다. 이러한 실험 결과를 바탕으로 땅나리와 섬말나리의 주·야간 발아 최적 온도는 20/20°C이며 GA3 24 시간 처리는 섬말나리 종자 발아에 효과가 없는 것으로 판단 되었다.