This study was conducted to find out the environmental, service sire and genetic effects for reproductive trait in certain purebred of pigs on Landrace and Yorkshire, and to suggest selection indicator which is to improve genetic capability on reproductive traits. There are five traits used on this analysis which are total number of born (NBT), number of born alive (NBA), piglets weight within litter (LW), average of birth weight on piglets within litter (ABW) and variation of birth weight on piglets within litter (VBW). With these data, the mixed model was established using 10,342 records collected from 2,527 sows of Landrace and 13,817 records collected from sows of 3,056 Yorkshire breeds and the variation of random effects and the genetic parameter were estimated by the REML method including service sire effects, permanent environmental effects and sow genetic effects. Due to characteristics of closed nucleus herd for using data on this study, given that it has been isolated breeding for about 19 years that progressed over 16 generations, genetic analysis was performed on all of these data and partial data of the current genetic group in which animals were born after 2011. The effects of service sire were estimated to be less than about 8% of total variation in all traits considered in the analysis. Permanent environmental effects were estimated about 2~14% of total variation in all traits considered. The heritability, which is the ratio of genetic variance among the total variance, was estimated to be 20~35% for LW and ABW in Landrace and Yorkshire, while it was about 10~14% for NBT. The genetic correlations between NBT and LW were 62~74% and between NBT and ABW were –28~-7%. Therefore, indirect selection for improving litter size could be possible with considering LW. Whereas, the genetic effect of the service sire effects for litter traits would be trivial.
새로운 유전자원을 창출하기 위해 풍산나물콩에 EMS를 처리한 후 돌연변이 집단을 육성하고, 돌연변이 계통 중 지방산 함량에 대한 변이체를 찾아 콩 유전육종 재료를 선발하고자 실시한 결과를 요약하면 다음과 같다.M2 3,744개체중 형태적 변이를 보이는 1,000개체를 선발하여 M4 세대에서 난쟁이형 (3.3%), 엽형변이 (2.6%), 엽록소결핍 (1.5%), 꽃색변이 (1.1%), 엽형변이를 보이는 난쟁이형 (0.2%)의 변이가 관찰되었다. 야생형인 풍산나물콩의지방산 함량은 palmitic acid 11.6%, stearic acid 3.4%, oleicacid 25.3%, linoleic acid 52.0%, linolenic acid 8.1%를 나타내었다. M4 892 개체의 종자지방산 함량을 분석한 결과palmitic acid, stearic acid, oleic acid, linoleic acid, linolenicacid 함량은 각각 7.4~19.7%, 2.2~13.0%, 14.7~49.0%, 31.8~63.9%, 3.9~15.9%의 범위를 나타내었고, 10.8%, 3.8%25.3%, 52.0%, 8.1%의 평균을 보였다. 각각의 지방산 별로 선발된 돌연변이 개체들은 다음과 같다. 고 palmitic acid함량을 나타내는 PE1542 (17.1%), PE3058 (17.0%), 고 stearicacid 함량을 나타내는 PE977 (12.7%)와 저 stearic acid 함량을 나타내는 PE2166 (1.9%), 고 oleic acid 함량을 나타내는PE450 (44.4%), PE2742 (47.7%), PE3058 (33.4%), 저 linolenicacid 함량을 나타내는 PE594 (4.6%), PE1690 (3.7%)와 고linolenic acid 함량을 나타내는 PE2166 (12.6%) 등이 선발되었다.
A basidiomycetous wood rotting fungus Pleurocybella porrigens (angel wings) has been well known as an excellent edible mushroom and named as Sugihiratake, in Japanese common name based on its morphological and ecological features. This mushroom has been favorably consuming by Japanese people. In 2004, an outbreak of serious acute encephalopathy exclusively occurred in patients with chronic kidney diseases after the intake of this mushroom. Thereafter, many researchers have conducted to examine the factor of onset of the encephalopathy, but the exact factors that induced the encephalopathy still remain unclear. Matsumoto et al. (2005) reported the mushroom specimens from different geographical areas in Japan were grouped into two distinct clusters based on ITS rDNA sequences. Vegetative growth of P. porrigens is extremely slow even cultivation on natural media surveyed and its physiological characteristic gives a disadvantage for the development of various fields of researches on this mushroom. Thus, we attempted to find a medium accelerating the vegetative growth of P. porrigens for further research to elucidate the possible association between its chemical constituent(s) and the onset of encephalopathy. Fifteen isolates of P. porrigens collected from different geographical areas in Japan were cultivated on PDA. The growth of the isolates was extremely slow as we know and most tested isolates formed mycelia look like those often observed in the cultivation of basidiomycetous ectomycorrhizal fungi. Among them, we chose five isolates based on the ranking of their higher growth rates. The top five isolates were cultured in five kinds of liquid media, PD medium, MY medium, Carrot medium, Ohta’s medium for ectomycorrhizal fungi (Ohta, 1990) and Amazake medium (Ishihara, 2007), and on a solid medium, PDA, at 20℃ in the dark. The dry biomasses of the isolates cultured in the liquid media were determined after 6 weeks of the stationary cultivation whereas the growth rates of the isolates cultured on PDA were compared by the colony diameter after 8 weeks of the cultivation. Among the tested liquid media, PD medium was the most suitable medium for their biomass growth (17 mg dry mycelium/flask - 127 mg dry mycelium/flask) and followed by Ohta’s medium (13 mg dry mycelia/flask - 79 mg dry mycelia/flask), MY medium (10 mg dry mycelium/flask - 72 mg dry mycelium/flask), Amazake medium (8 mg dry mycelium/flask - 47 mg dry mycelium/flask) and Carrot medium (4 mg dry mecelium/flask - 18 mg dry mycelium/flask). The average biomass growths of the isolates cultured in the synthetic medium, Ohta’s medium were 20-92% of those of the isolates cultured in PD medium. No correlation was observed between the cultivation in PD medium (liquid medium) and that on PDA (solid medium). Extremely large biomass variation was observed among the same isolates cultured on the same kind of the liquid medium. Large variation in colony features associated with their growth rates was also observed among the isolates cultured on PDA. These results suggest that PD medium would be a suitable one for most researches and Ohta’s medium for researches requiring clarity of its chemical constituents. The above results also indicate that the Japanese population of P. porrigens has large variation in vegetative growth although we have not yet examined whether the tested isolates comprise cryptic species or not.
옥정호는 1994년이후 가두리 양식장이 감소하기 시작 하여 98년도에 모두 철거되었으며 현재 상수원 보호구역 지정검토등 많은 노력을 기울임으로 수질개선에 노력하고 있으나 상류하천으로부터 오염물질이 계속 유입되고 있어 수질상태가 현격히 좋아질 것으로 기대되기는 어려운 실정이다. 1998년도에 조사한 옥정호 수질자료를 분석한 결과에서 COD, T-N 값은 조금 높아지는 경향을 나타냈으며 SS, T-P, 투명도 등은 예년과 비슷하거나 경우에 따라서는 조금 낮은 수준으로 평가되어 전반적으로 예년과 유사한 것으로 평가되었다. 그러나 영양상태를 평가하는 여러 가지 기준에 따라 수질상태를 평가해보면 옥정호는 시기에 따라 또는 기준에 따라 약간의 차이를 보이기는 하나 대체로 중영양상태와 부영양상태를 나타내는 것으로 평가되어 조속한 대책을 수립 시행하여야 할 것으로 판단되며, 특히 유입하천 조사에서는 임실천의 수질상태가 다른 조사지점에 비해 오염도가 높게 조사되어 임실천에 대해서는 확실한 오염원대책이 강구되어야 할 것으로 평가되었다.
이상에서 본 연구는 1960년 이후부터 1985년까지의 인구규모와 그 변동의 특질을 농촌에서 도시로의 인구이동현상과 관련하여 검토해 보았다. 아울러 분해시계열방법을 이용하여 농촌인구유출과 농정과의 관계도 고찰하였다. 그리고 적정모델을 추정하여 2,000년까지의 농촌 및 농가인구도 예측해 보았다. 결론적으로 말해서 인구구조의 변화측면에서 농촌인구와 농가인구는 1965년부터 감소하기 시작하여 1975년 이후부터 급격히 감소하였다. 농촌인구의 급격한 감소는 농촌 인구의 자연감소로 인한 요인보다는 도시로 유출되는 인구이동의 요인이 더 크게 작용한 것으로 나타났다. 농촌에서 도시로의 인구유출과정에서 유입지의 분포를 보면 도시지역 중에서 서울, 부산, 경기지역으로 인구가 집중한 것으로 나타났다. 특히 70년대에는 서울 부산 등 대도시 지역으로 직접적인 이동이 있었으나 80년대에 들어와서는 대도시 지역으로의 직접적인 유입보다는 주변지역으로의 유입이 점차 증가함으로써 농촌에서 대도시로의 유입형태가 주변도시로의 유입형태로 변화되고 있다. 농촌인구의 유출을 농업생산과 관련시켜 볼 때 농업노동력의 수요가 확대되는 6월 이후에 유출인구는 점차 줄어들고 있으며 3월에 가장 많이 유출하는 것으로 판명되었다. 60년대 이후 농업정책과 농촌인구의 유출과의 관계를 분석하기 위해 순환변동을 도출하였으며 2-3차 개발계획기간을 제외한 기간에 있어서 농촌부문의 상대적 저위성이 농촌인구의 유출을 자극한 요인으로 작용했으며 이러한 사실은 농업정책의 불균형적 시행이 직 간접적으로 영향을 미친 결과라 하겠다. 끝으로 농촌인구의 유출을 포함한 한국농업의 근본적인 문제는 도농간 상대적 소득 격차에 있다고 보고 농촌지역의 개발을 서울이나 수도권, 대도시권의 비대화를 막기 위한 방편 또는 공업화 우선정책의 반작용으로 본 과거의 정책성향을 극복해야 한다는 점이다. 따라서 도농간 소득격차의 문제를 농업정책의 중심과제로 삼고 농촌지역의 자원을 보다 효율적으로 이용함으로써 나라의 경제적 효율성은 물론 형평성을 높인다는 시각에서 농업정책을 다루어야 할 것이다.
The population variation of Spanish mackerel (Scomberomorus niphonius) according to its major prey abundance was analyzed using monthly catches of coastal set net fisheries in the southern waters off Gyeongsangnam-do and eastern waters off Gyeongsangbuk-do of Korea from 2006 to 2019. The abundance of Spanish mackerel and its prey species fluctuated almost simultaneously with time lags of +2 to -2 months between the set net fisheries in the southern and eastern waters. The generalized additive model revealed that the abundance of Spanish mackerel was influenced by its prey species such as hairtail and anchovy in southern waters, and common mackerel and horse mackerel in eastern waters. The model deviance explained 49% and 42% of Spanish mackerel abundance in southern and eastern waters respectively. These results suggest that the abundance of Spanish mackerel is affected by seasonal migratory prey fish species in the coastal areas and can be linked to their northerly migration.
The exposed population of a vulnerable group to high ozone episodes (exceeding 60 ppb/8h) was estimated in Busan metropolitan city from 2000 to 2010. The frequency of high ozone days at monitoring sites and the number of the population aged over 65 were used to calculate the accumulated (total, seasonal, and yearly) number of the exposed older population (EOP) to high ozone episodes during the study period based on administrative areas, by interpolation and zonal mean methods in ArcGIS software. The older population in this city had increased significantly from 2000 to 2010 (representing over 10% of the total population in 2010). The vulnerable areas (e.g. the eastern area of the city) of the EOP to high ozone episodes were different from the areas with frequent high ozone episodes (e.g., the western area) due to the increase of the older population in particular areas. The difference was more significant in spring than in any other season, and in 2010 than in previous years (2000 and 2005).
Mutant analysis is one of most optimized genome-wide approach towards acquiring utile phenotypes and defining related genes. Gamma-irradiation, an acknowledged way of mutant-generating method, was applied to gain sets of mutant line in Brachypodium distachyon. B. distachyon is a model plant, commonly used in genus of Gramineae for the research of structure genomics and functional genomics. B. distachyon contribute to rapid and easy analysis because of its small size and quick growth. Mutant population was generated by different doses of gamma-irradiation (0, 50, 100, 150, 200, 250 Gy) in the gamma field phytotron. Distance from the source gives same irradiation duration for each plant. Plant growth parameters such as plant height, tiller number, leaf length & width, internode number & diameter, maturity and yield components (ear number biomass) were scored on M0 plants. Plant responses to different doses of radiation are evaluated and the effective radiation dosages to generate mutant using gamma-phytotron are suggested. Chronic irradiation using gamma-phytotron is useful tool to generate mutants for genomic variations such as SNP or INDEL as well as suitable for functional study of genes in Gramineae.
설악산 눈잣나무 천연집단은 국내 유일하게 분포하는 남방한계 분포지 수종으로 유전자원보존을 위하여 침엽의 형태적 해부학적 특성과 I-SSR 표지자를 이용한 유전변이를 조사하였다. 8가지 침엽특성에 대한 ANOVA 분석결과 모든 특성에서 개체 간에 통계적인 유의성이 인정되었다. 조사된 66개체의 평균특성은 침엽길이 53.59 mm, 침엽폭 0.78 mm, 침엽지수 68.98 및 침엽두께 0.65 mm, 최대기 공열수 4.56개, 최소기공열수 3.80개, 전체기공열수 8.36개 및 수지구수 1.71개로 각각 나타났다. 특히, 수지구는 모두 바깥쪽에 위치하는 외위 형태의 1~3개 범위로 수지구 2개 유형이 69.47%을 나타낸 반면 1개 유형은 30.45%를 나타냈다. 선발된 9개 I-SSR Primer에서 총 78개의 증폭산물을 얻었으며, 다형적 유전자좌의 비율은 61.5%, 유효대립유전자의 수 1.698개, 이형접합도의 기대치 0.388 및 Shannon의 다양성지수 0.567로 각각 나타났다.
우리나라에 제주도와 남부도서지방 등지에 자생하고 있는 새우란을 신품종으로 육성하기 위 하여 우량계통을 1차 선발한 57개의 우수계통 중에서 화려한 꽃색과 향기를 지니고 있어 원예적 가치가 인정되어 신품종으로 선발 육성한 ‘색동’의 품종특성을 보면 다음과 같다. 색동의 꽃받침색은 밝은 주황색이고 꽃잎은 짙은 노란색으로 화려한 화색을 가졌으며, 순판색은 노란색으로 화색이 화려한 변이종이었다. 또한 방향성을 지니고 있었고 꽃피기는 안아피기 형태를 가지고 있었다.
자연교잡 실생 작약집단내에서 지상부 및 지하부에 관련하는 각형질의 특성과 분포를 조사한 결과는 다음과 같다. 자연교잡 실생 작약집단의 지상부생육은 맹아기가 4월 1일~4월 15일, 경장은 80.0~21.0cm, 주당 경수는 15~3개로 분포가 다양하였다. 지하부의 근장은 59~12cm, 주당 근수는 73~4개였으며, 주당 약근 수량은 882~37g으로 개체간 변이가 심하였고 750g이상 되는 대형도 7.1% 분포하였다. Paeoniflorin함량은 10.29~1.9%로 7.5%이상의고 Paeoniflorin을 가진 개체도 14.3%분포하였다. 근장은 경직경과, 뿌리수량은 경장 및 뿌리직경과 각각 고도의 유의한 정의상관이 있었다.
Somaclonal variation was observed in the field on doubled haploid plants derived from single pollen of a rice cultivar "Hwaseongbyeo". The variations of seven quantitative traits including plant height and one qualitative trait (pubescence) in 436 lines (R2 generation) were analyzed. The number of lines which fell beyond the boundaries of the 95% confidence intervals of the check variety, Hwaseongbyeo was checked for each quantitative trait, and of those fertility showed the highest variation frequency (85.6%), followed by plant height (77.5%), flag leaf length (66.5%), grains per panicle (42.2%), days to heading (34.5%), panicle length (30.7%) and panicles per hill (22.7%). And the variations of quantitative traits except days to flowering appeared to move in the negative direction compared to "Hwaseongbyeo". Variability within lines was also observed for quantitative and qualitative traits. Twenty-nine R2 lines (7%) segregated for pubescence and 130 R2 lines (30%) showed variation with regard to fertility. This suggests that mutations usually occur before diploidization. Twenty-nine R2 lines representing a wide spectrum of variation were chosen for RAPD analysis. The number of lines showing DNA polymorphism compared to Hwaseongbyeo ranged 0 from to 10 according to the primer used and this seems to indicate that specific loci have highly mutable genomic site.utable genomic site.