This study is intended to examine the tDCS and Morris Water maze training in Alzheimer’s disease(AD) rats on Tau protein expression. Experiment groups were divided into four groups and assigned 16 rats to each group. Group Ⅰ was a control group(AD induced by scopolamine); Group Ⅱ was a experimental control group(AD injured by scopolamine and treatment tacrine); Group Ⅲ was a group of tDCS application after AD injured by scopolamine; Group Ⅳ was a group of morris water maze training after AD injured by scopolamine. In cognition test, the outcome of group Ⅱ was significantly lower than the groups(p<.001). and group Ⅲ, Ⅳ were significantly low result at 14 days(p<.05). In histological finding, the experimental groups were destroy of micro vessels and finding of cell atropy and swelling. Group Ⅲ, Ⅳ were decreased in degeneration of liver and kidney cells. In immuno- histochemistric response of BDNF and tau protein in hippocampus, BDNF expression of Group Ⅱ was more increase than the other groups. and increase of BDNF expression was Ⅲ, Ⅳ were higher than group Ⅰ at 21 days. Tau protein expression of Group Ⅱ was more decrease than the other groups. and decrease of Tau protein expression was Ⅲ, Ⅳ were lower than group Ⅰ at 21 days. These result suggest that improved tDCS and morris water maze training after scopolamine induced is associated with dynamically altered expression of BDNF and Tau protein in hippocampus and that is related with cognitive function.

This study aims to reveal how EA affects BAX and NF-kB involved in cell deaths from global ischemia, and to do this, observes the changes of BAX and NF-kB caused by EA application after transient global ischemia. The experimental method is to give rise to global ischemia and apply EA to 27 SD rats with the particulars of being six-week-old, male, around-300 gram-weighing, and adapted to laboratory environment for more than a week, and divide them into three groups, that is, GV20 EA group(n=9), L14 EA group(n=9), no-treatment GI group(n=9), and then observe their changes of BAX and NF-kB at the time lapse of 6 hours, 9 hours and 12 hours after ischemia, using western blotting. The numerical decrease of BAX expression at the time lapse of 9 hours after EA application, though not statistically significant, was observed in GV20 EA group and L14 EA group, and the NF-kB expression appeared statistically significant decrease in GV20 EA group and L14 EA group, but the expression was higher in the group with EA application. Therefore, EA application at the early phase of global ischemia is considered to affect BAX and NF-kB and play a positive role in decreasing apoptosis and cell deaths by inflammation.

The aims 0 1' t his study we re to evaluate proliferative and apoptotic cell distribution pattern in condylar articu lar and pl'oliferative layer as two types of diet(soft and ha rd) we1'e supplied to growing 1'a ts, 30 male Sprague Dawley g1'owing l'ats(twenty-one c1ays fl'om bi l'th) were c1ivided into two group, Each 15 rats were supplied by soft and hal'd di et , After c1 iluted Bl'du solution(5mg/ Kg)was injected into pel'itoneum befol'c sacrificc. rats wel'e sacl'i f iced at 1. 2. 3 week llltel'vals St1'eptoavidin-Biotin method for Brdu was used to evaluate celluJar proliferative activity. ancl fluol'escent TUNEL method was used to estima te the apoptotic activity, The results about this expe riment wel'e recorded about ante1'io1' and posterior condyle separateJy, In anteriol' portion of condyle, soft diet group showed inc1'eased p1'olife1'ative celluJar activity tha n hal'd diet group dw'ing 1, 2 week but decl'eased than hal'd diet g1'oup at 3 weeks. while hal'd diet group showed constant p1'oliferative cellular activity during all experimenta l period , ln posterior pOl'tion of condyl e、soft diet group showed increased activity than hard diet group at 3 weeks, while ha rd di et gl'oup showed constant proliferative cellular activity during exper‘ imental period , In a nterior pOl'tion of condyle‘ soft c1 iet group showed increased apoptotic activity with time progress, but hard diet group showed continuous low level of activity during experimental period , In posterio1' porti on 0 1' condyle, hard diet group showed cons tant low level apoptotic activity, plthough showed the lowes t leveJ at 1 week, On the con trary soft diet g1'oup showed c1ecl'eased apoptotic activity wi th time progress, ConcJusively, in soft diet group an teroposterior direction was reduced in condyJar morphologic dimension because of decreased prol iferative cellular and increased apoptotic activity on anterior portion and vertical dimension 0 1' condyle was increased because of increased proliferative cellular and decreased apoptotic activity on posterior portion. but in hard di et group proli ferative cellular and apoptotic activity were comparatively constant. and thus harcl diet group s howed antet'opos teriorJy broad and flat condylar morphoJogy

Von Ebner's glands (vEG) are minor salivary glands associated with circumvallate and foliate papilla. The secretions of vEG consist of microenvironment of the taste buds in the circumvallate and foliate papillae, and thus saliva from vEG plays a role in the perception of taste. The Ca²+ signaling system in rat vEG acinar cell was examined using the Ca²+-sensitive fluorescent indicator Fura-2. Agonist-induced increase in intracellular Ca²+([Ca²+]i) was stimulated by carbachol (CCh) and substance P (SP), but not by norepinephrine (NE), and recovered to control levels by their receptor antagonists dose-dependently. The effects were also observed in Ca²+-free medium, suggesting mobilization from intracellular Ca²+ store. These results in the vEG acinar cell indicate that 1) [Ca²+]i is at least regulated by muscarinic and neurokininergic (NK1) receptors; 2) the increases in [Ca²+]i activated by CCh and SP are mainly mediated by discharge of cytosolic calcium pool.

This study was attempted to observe the effect of Job's tear diet on change of body weight and tissue in rat. The changes of body weight were higher in Job's tear non-diet group(A) and seemed to lower in Job's tear diet group(c) as expected. The weight of each organ appeared to heavy more C group than A,Bgroup in Brain, Spleen. Lungs and Kidney. Liver was heavier in Job's tear to diet one day among six day group(B) and ovary was lighter in C group, The levels of each organ TBA-value were higher in Brain, Liver of A group and showed to higher Spleen, Lungs, Ovary of C group TBA-value of Kidney was revealed to lower more C group than A,B group.

Our country has been produced much amounts of panax ginseng roots which has a stimulating effects on the metabolism of protein, lipid and nucleic acids in the body. And the leaf trunk of panax ginseng were also produced a considerable amounts as the by - products. Therefore, this study was devised to observe the nutritional effect to rats feeding of rice diet supplemented with by - products of panax ginseng, male Albino rats of pure strain weighing 73.8 ± 0.7 g were used as experimental animal to investigate the changes of cholesterol in heart and testis. The animals were divided into sixteen diet group, they were the protein contents of 9%, 12%, 15% and 18% supplemented with 2% panax ginseng roots and its by - products respectively. The group without the supplements were used as the control. The diet group were again divided into 2 groups according to the feeding terms, 4 weeks and 8 weeks. It is concluded that the free from cholesterol and total cholesterol contents in the heart and testis with the supplements of panax ginseng roots and its by - products showed significant difference compared to the control group.

Eleutherococcus senticosus Maxim. has been successfully used as an oriental medicine for various diseases including allergic disorders. Histamine is a major factor on various allergic responses and it is reported that histamine was released from mast cells by sensitization of allergens. In this study, ethanol extracts from E. senticosus Maxim. were prepared and the composition was analyzed by high performance liquid chromatography. The eleutheroside B as a primary effective component of E. senticosus was contained approximately 225 mg/kg in root bark extracts. The extracts were found to significantly inhibit compound 48/80-induced histamine release form mast cells in dose dependent manner. However the extracts had low cytotoxicity on the mast cells with MTT assay. These results showed that E. senticosus Maxim. extracts may be the effective materials on inflammatory disorders.

수삼 추출물에서 홍삼특이 사포닌 성분의 함량을 증대시키고자 추출온도 및 시간에 따른 사포닌 성분별 함량 변화를 조사하였다. 수삼을 85℃에서 48시간 추출 시 총 사포닌 함량이 23.5 mg/g(D.W.)로 가장 많았으며, 본 조건에서 추출한 인삼추출물의 항알러지 기능성을 검토하고자 랫트 비만세포에서 compound 48/80로 유도된 히스타민 유리 작용을 억제하는 인삼추출물의 효능을 분석하였다. 인삼추출물의 비만세포에 대한 세포 독성 시험 결과 각 추출물은 수시간 노출시 높은 세포 생존율을 보이고 있었고 비교적 고농도인 0.5 mg/ml 처리군에서도 80% 이상의 세포 생존율을 보였다. 인삼추출물의 히스타민 유리 억제 효과를 조사하기 위해 랫트 비만세포에 대표적인 탈과립 유도제인 compound 48/80을 단독 또는 각 추출물을 동시에 처리한 후 얻어진 상청액의 히스타민의 정량을 실시한 결과 비만세포는 compound 48/80의 처리 조건에서 대조군에 비하여 30% 가량의 히스타민 유리량이 증가하였는데, 인삼추출물 처리 시 히스타민 유리 수준이 정상 수준에 가까이 감소하는 것을 확인하였다. 이는 인삼추출물의 항 알러지 목적으로의 이용 가능성을 제시하고 있다.

Mesenchymal stem cells constitute an potential cellular source to promote brain regeneration with Parkinson's disease. Mesenchymal stem cells have significant advantages over other stem cell types and greater potential for immediate clinical application. The purpose of this study was to investigate whether hMSCs from the human adipose tissue could be induced to differentiate into dopaminergic cells and to assess the developmental potential of hMSC for selectively replacing the midbrain dopamine neurons lost in Parkinson's disease in vitro and in vivo. MSCs were cultured under conditions that promote differentiation of dopaminergic neuron. Using media that include SHH, FGF8, and GDNF. the MSCs were induced in vitro to become dopaminergic neurons. The expressions of the LIM homeobox transcription factor 1, alpha (Lmx1a), tyrosine hydroxylase(TH) proteins were determined by immunofluorescence. Lmx1a has been shown sufficient to confer neurogenic activity on mesencephalic floor plate cells and to determine a mesencephalic dopaminergic neurons fate. This result suggests that hMSCs have the ability to differfentiate into dopaminergic neurons. hMSCs were then transplanted into the striatal in a rat model of Parkinson's disease. The rats were unilaterally lesioned in the substantia nigra with 6-hydroxydopamine and were tested for rotational apomorphine-induced behavior. Following differentiation of dopaminergic neuron, cells displayed dopaminergic morphology and that they expressed dopaminergic marks genes. Finally transplantation of hMSCs into the striatal of Parkinsonian rats resulted in improvement of their behavioral deficits by apomorphine-induced rotational behavior. The hMSCs transplanted rats were proved to be better than compared with the transplantation of PBS. Immunohistochemical analysis of grafted brains revealed that abundant hMSCs survived from the grafts and some of them displayed dopaminergic marks. Our results indicate that hMSC may serve as a good cell source for the treatment of neurodegenerative diseases and have high potential for being used in multiple applications. This cellular approach might become a restorative therapy in Parkinson's disease.

Main strategy for a treatment of Parkinson's disease (PD), due to a progressive degeneration of dopaminergic neurons, is a pharmaceutical supplement of dopamine derivatives or ceil replacement therapy. Both of these protocols have pros and cons; former exhibiting a dramatic relief but causing a severe side effects on long-term prescription and latter also having a proven effectiveness but having availability and ethical problems Embryonic stem (ES) cells have several characteristics suitable for this purpose. To investigate a possibility of using ES cells as a carrier of therapeutic gene(s), human ES (hES, MB03) cells were transfected with cDNAs coding for tyrosine hydroxylase (TH) in pcDNA3.1 (+) and the transfectants were selected using neomycin (250 ). Expression of TH being confirmed, two of the positive clone (MBTH2 & 8) were second transfected with GTP cyclohydrolase 1 (GTPCH 1) in pcDNA3.1 (+)-hyg followed by selection with hygromycin-B (150 ) and RT-PCR confirmation. By immune-cytochemistry, these genetically modified but undifferentiated dual drug-resistant cells were found to express few of the neuronal markers, such as NF200, -tubulin, and MAP2 as well as astroglial marker GFAP. This results suggest that over-production of BH4 by ectopically expressed GTPCH I may be involved in the induction of those markers. Transplantation of the cells into striatum of 6-OHDA- denervated PD animal model relieved symptomatic rotational behaviors of the animals. Immunohistochemical analyses showed the presence of human cells within the striatum of the recipients. These results suggest a possibility of using hES cells as a carrier of therapeutic gene(s).