This study was conducted to see the initial increasing time of population of citrus leafminer(CLM) in spring and early summer seasons in 2007-2011. CLM adults were monitored using sex pheromone trap which is mainly composed of Z,Z-7, 11-hexadecadienal in the 4, 8 and 5 orchards of the east, south and north part in jeju island, respectively during 2007-2011 seasons. The traps and lures were changed at 10 days and 1 month interval, respectively. Though CLMs were captured on traps from March or April, it was 17, 28 and 28 May in 2007, 16, 26 May and 5 June in 2008, 8, 26 and 26 May in 2009, 27 May, 7 and 7 June in 2010 and 25 May, 2, 2 June in the south, east and north part of Jeju Island, respectively that the CLM population rapidly began to increase toward peaking. The accumulated temperature except less than developmental zero, 12.1 Ujiye T 1990). from January was 248.0, 270.5 and 289.7 in 2007, 193.2, 203.8 and 261.4 in 2008, 202.0, 245.5 and 259.9 in 2009, 261.4, 192.2 and 268.3 in 2010 and 243.4, 246.3 and 252.4 in 2011 at that time. After May overwintered CLM adults are little possible to be counted to the number of captured CLM because adult longevity is about 100 in degree days (Lim 2006). CLM populations in the south part of Jeju Island in first CLM adults increasing periods during 2007-2011 is higher than other parts.

The box tree pyralid, Glyphodes perspectalis, was reared on an artificial diet based on dried powder of box tree leaves, Buxus microphylla var. koreana, and INSECTA F-II (Nihon Nosan Co., Ltd) at a ratio of 3:7 (w:w) and analyzed in their developmental characteristics from 1st to 4th generation. The developmental period of larva reared on artificial diet was longer than that of larva reared on host plant. Significant difference in larval period was also observed between 1st and other generation groups. Pupation rate was the highest in 4th generation followed by 3rd, 2nd, 1st and host plant group. Significant difference in pupal period was noted between 1st and 3rd generation group. Emergence rate was the highest in 4th generation group followed by 3rd, host plant, 1st and 2nd generation group. There is a significant difference in male and female size between 1st and 4th generation group. Our result indicated that stable rearing of box tree pyralid through the year is possible by artificial diet, and the best adaptation to artificial diet was achieved at 4th generation. The pheromone components of this moth have been identified as (Z)-11-hexadecenal (Z11-16:Ald), (E)-11-hexadecenal (E11-16:Ald), and (Z)-11-hexadecenol (Z-11-16:OH). We did several field tests, and found that there was a difference between Korea and Japan population.

In haplodiploid sex determination, females are sexually reproduced from fertilized diploid eggs, and males from unfertilized haploid eggs. Haplodiploid sex determination seems simple in that sex depends simply on the ploid level. However, the underlying genetic mechanisms are thought to be much more complicated than expected. Among them, a powerful proposed mechanism is genomic imprinting. All epigenetic on-off systems require target genes, unless the systems target histone proteins on chromosomes. For Hymenoptera, a good candidate target gene in terms of sex determination is known either as feminizer (fem) or transformer (tra) in many insects. These two genes are essential for expressing femaleness. In most Hymenopteran insects, the maternal tra seems to be methylated and consequently not expressed, while the paternally derived tra gene is not methylated. Therefore, a fertilized egg with the paternally derived active tra gene will develop into a functional female. Like all Hymenoptera, ants (Formicidae) have haplodiploid sex determination. In Vollenhovia emeryi, however, queens are produced clonally while workers derive from fertilized eggs. Males are haploid, likewise deriving from fertilized eggs, but only after selective elimination of their maternal genome. Under the conventional genomic imprinting model, we would have expected that the opposite pattern of what is observed in others. Here we present extraordinary sex determination and suggest our hypothesis about genomic imprinting pattern in V. emeryi

This study evaluated a method of sorting X and Y chromosomes based on size using the forward angle light scatter related refractive index (FSC) of a flow cytometer. Hanwoo bulls sperm were separated to X and Y chromosomes by the parameters of FSC or Hoechst 33342 intensity. As a result, using monitor program linked flow cytometry during sorting processing, the purities were or for the X-fraction and or for the Y-fraction in the two sperm sorting methods. There were no differences in the X and Y ratios (X and Y %) between the sperm sorting methods based on FSC or DNA content. The proportions of female and male embryos used for in vitro fertilization and development were or , and or when sperm were processed using the sex sorting method by FSC or Hoechst 33342. In conclusion, further study is needed to determine the optimum procedure and improve the nozzle to enhancing sorting accuracy or efficiency. Also, the findings of this study do not negate the possibility that the difference method of sperm sorting cannot use a UV laser beam.

In mammals, the meiosis division in testes produces equal numbers of two different types of gametes: X chromosome-bearing sperm (X-spermatozoa) and Y chromosomebearing sperm (Y-spermatozoa), which have equal potential to fertilize the oocytes. Therefore, the expected 1: 1 sex ratio is observed. However, under some conditions like endocrine disruptors (EDs) exposure the sex ratio is deviated than the expected with more males or more females. And recently many hypotheses have been postulated to explain the mechanism of sex ratio deviation; however none of them introduced a proven experimental explanation. To solve this enigma, we hypothesized that the differences between X- and Y-spermatozoa survivability under specific conditions due to differences in their chromosome contents are the key leading to the sex ratio alteration. To examine our hypothesis, we combined two techniques; first, hypo-osmotic swelling (HOS) test that was applied to test viability of spermatozoa and second, fluorescence in situ hybridization that was applied on HOS-treated spermatozoa to define sex chromosome composition. In the present study, human spermatozoa were incubated with a group of EDs represent a widespread chemicals in the environment bisphenol A (BPA, 100 μM), nonylphenol (NP, 10 μg/ml), 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD, 2.5 μg/ml), genistein (Gen, 100 μM), and the following pesticides, dibromochloropropane (DBCP, 10 μg/ml), atrazine (Atraz, 500 μM), and diazinone (Diaz, 500 μM) for 6 hr at 37℃ in 5% CO2. Then, the viability of spermatozoa and their sex chromosome contents were evaluated simultaneously. Among seven chemicals studied only four chemicals (Atraz, DBCP, TCDD, and Diaz) significantly decreased Y-sperm viability when compared to those of X-spermatozoa in the same treatment group and viability of Y-spermatozoa when compared to those in the negative and positive (DMSO) control groups (p<0.05). Also, in these four treatment groups the sex ratio of live sperm population was significantly lowered compared to the control groups (p<0.05). Otherwise, Gen, BPA, and NP did not show any significant effect on viability of Yspermatozoa or decreasing sex ratio in live sperm population as compared to the control groups. It has been proven that TCDD, DBCP, and the pesticides decrease the sex ratio, but the same effect was not observed in case of Gen, BPA, and NP. From the present findings, there is no doubt that the EDs may alter sex ratio via decreasing Y-spermatozoa viability.

The α-proteobacterium Wolbachia is maternally inherited and is known to induce reproductive distortions in a wide range of insect taxa such as cytoplasmic incompatibility, feminization, male killing and parthenogenesis (PI). When a female is infected with PI-Wolbachia, she does not need a male to produce female offspring, because the female can produce female offspring via gamete duplication without the aid of sperm. However, in the parasitoid wasp Trichogramma kaykai species, Wolbachia infected parthenogenetic females still produce a fraction of male offspring. Offspring sex ratio for 10 days are different according to each T. kaykai isofemale line that is infected with PI- Wolbachia. This may be caused by complex interactions between Wolbachia and the host genetic backgrounds on converting sex of infected eggs.

Eight female Himalayan tahrs (Hemitragus jemlahicus) were estrus-synchronized, and transcervically inseminated with frozen-thawed semen in September, 2009, about 2 to 3 months earlier than their natural breeding season. Intravaginal progesterone-releasing devices were inserted into vaginas of six Himalayan tahrs on September 7, and the other two on September 8 to suppress luteal function of ovaries. The devices had been placed deep inside the vagina prior to withdrawal on September 23. A day before CIDR removal, a combination of PMSG 400 IU and hCG 200 IU was intramuscularly injected. Forty hours later, frozen-thawed semen was transcervically inseminated. Pregnancy diagnosis was performed 39 days later by analyzing progesterone level of serum. Every treatment was done under anesthesia inducted by xylazine injection. In conclusion, vaginal discharge of cervical mucus, hormonal changes induced by implant-typed or muscularly injectable hormones and widening of cervix enough to insert an insemination gun into uterine body were achieved in non-breeding season. Moreover, the first inseminated Himalayan tahr, 36 hours after CIDR removal was assumed to be pregnant but the fetus may have been lost due to the use of anesthetic drug.

Spermatozoa sorted by flow cytometry have been successfully used to produce offspring in domestic animals and are commercially available for cattle. Also sheath fluid is the important environment for viability of sex-sorted sperm in flow cytometry. The aim of this study was to investigate whether or not HEPES (N-2-hydroxyethylpiperazine-N'-2-Ethanesulfonic acid) has any effect on the viability in sex-sorted Hanwoo (Korean native cattle) sperm. In this study, the semen was collected from Hanwoo of Hoengseong Livestock Cooperation by artificial vagina method then pooled and subjected to cryopreservation in straws. Sperm were cultured for 0, 30, 60 and 120 min with 0, 2.5, 5, 7.5 and 10 mM of HEPES added to the sheath fluid and incubated at 4, 20 and 38, respectively. For the cytometric analysis the frozen-thawed semen was extended with 5 mM HEPES extender to final concentration ( spermatozoa) at 4, 20 and 37. Sperm viability was assessed with SYBR-14 and propidium iodide (PI) staining. This study shows that the viability of sperm was decreased with prolongation of incubation time in all of test. But the viability of sperm which were treated with 38 was gently decreased than that of treated with other temperature. The viability of the control was sharply decreased (p<0.05) than all of the HEPES treatment group at 60 to 120 min in 38. X-sexed sperm was more sensitive than Y-sexed sperm to temperature during f10w cytometry (p<0.05). In conclusion, the results of this study suggest that the sheath fluid with 5 mM HEPES has effect on maintenance of viability after sperm sexing at 37 in Hanwoo.

Haplodiploid sex determination occurs in a wide range of animals, especially in Hymenoptera, where a fertilized egg develops into a diploid female and unfertilized into a haploid male. However, recent studies on diploid functional males in some wasps suggest that the simple addition of paternal gene by fertilization may not be enough to explain female offspring production in the sex determination system. Recently, activation of sex determination gene (tra) was found to have a pivotal role in determining the sex of Nasonia vitripennis. In N. vitripennis, tra is activated only on the paternal genome (i.e. sperm) not on the maternal counterpart (i.e. egg). Such parent specific activation of a gene is controlled by a epigenetic factor, DNA methylation. However, in Trichogramma kaykai, Wolbachia induces female offspring production without sperm. Therefore all female offspring are clonal to the maternal gene. This violates the role of activated sex determination gene (tra) from sperm in the wasp. We hypothesize that Wolbachia has an ability to activate the gene by demethylation. This hypothesis indicates that the target of sex ratio distorting endosymbionts may be an upstream gene. It will enhance our understanding of evolution of haplodiploid sex determination.

The α-proteobacterium Wolbachia pipientis infects a wide range of arthropods and filarial nematodes. Wolbachia is maternally inherited and is known to induce reproductive anomalies such as cytoplasmic incompatibility, feminization, male killing and parthenogenesis induction (PI). Trichogramma kaykai is a tiny wasp that parasitizes on lepidopteran eggs. When a female of the wasp is infected with PI-Wolbachia, the female produces female offspring via gamete duplication without the aid of sperm. As she ages, however, the fraction of male offspring increases. In this study, we investigated the effect of host genetic background on the expression of sex ratio between isofemale lines. Virgin females of six isofemale lines were allowed to lay eggs individually for 10 days. There was the positive relationship between female age and the offspring sex ratio. Furthermore, the sex ratio was significantly different among isofemale lines, implying that the host genetic background had an effect on the sex ratio. Based on the results, evolution of symbiosis in terms of sex ratio and future experimental design are discussed.

The Wolbachia bacterium is known to induce reproductive anomalies in various insect taxa such as cytoplasmic incompatibility, feminization, male killing and parthenogenesis. It is hypothesized that the degree of reproductive anomalies is dependent on the bacterial infection density. In this study, we attempted to test the hypothesis using the tiny egg wasp, Trichogramma kaykai that has served as the model system of parthenogenesis where an unfertilized egg develops into a female due to the bacterial infection. So far this is only found in haplodiploid organisms. The results show that 1) as mothers aged, they started producing male offspring, 2) the sex ratio was negatively correlated with the bacterial infection density, 3) female offspring were more than six times heavily infected with Wolbachia than male offspring in the species. In conclusion, female offspring production, parthenogenesis, is as a function of the Wolbachia bacterial density in this species.

The sex pheromone of Synanthedon bicingulata (Staudinger), a major pest of peach trees in many regions of northeast Asia, was identified. Two major components from the pheromone gland extracts of female moths are E3,Z13-18:OAc and Z3,Z13-18:OAc, and the average ratio of these components is about 4:6, respectively. In addition to the major components, four minor components, Z13-18:OAc, E2,Z13-18:OAc, E3,Z13-18:OH, and Z3,Z13-18:OH also were identified from pheromone gland extracts. Field tests showed that E3,Z13-18:OAc and Z3,Z13-18:OAc are essential for attraction of male S. bicingulata moths, and males are optimally attracted to the blend ratio found in pheromone gland extracts of conspecific females. Addition of the minor glandular components (Z13-18:OAc, E2,Z13-18:OAc, E3,Z13-18:OH, and Z3,Z13-18:OH) did not affect captures of males to the primary binary blend. Thus, the blend of E3,Z13-18:OAc and Z3,Z13-18:OAc at the natural ratio can be used for monitoring populations of this species.

In this study, we reared box tree pyralid, Glyphodes perspectalis, with artificial diet and identified sex pheromone. Insect F-II and leaf of box tree were used to make artificial diet. Box tree pyralid, Glyphodes perspectalis, is the most serious pest of box tree in Korea, and was recently introduced into Europe. The pheromone components of this moth have been identified as (Z)-11-hexadecenal (Z11-16:Ald), (E)-11-hexadecenal (E11-16:Ald), and (Z)-11-hexadecenol (Z-11- 16:OH) in Japan. In this study, we identified pheromone components of this species by using GC and GC-MS. Three same components, such as (Z)-11- hexadecenal (Z11-16:Ald), (E)-11-hexdecenal (E11-16:Ald), and (Z)-11-hexadecenol (Z-11-16:OH) were also indentified in Korean population, but there was a little difference in ratios. The ratios of these three compounds of Japanese population and Korean population were 5:1.25:1, and 5:0.96:0.2, respectively. The ratios of Z and E-11-hexadecenal were similar, but the ratio of (Z)-11-hexadecenol was lower compared to Japanese population. In field bioassay, (Z)-11-hexadecenal or (E)-11-hexadecenal alone was not attractive to males, but the mixture of Z11-16:Ald and E11-16:Ald was attractive to males. The most effective ratios of Z11-16:Ald and E11-16:Ald was 5:1. Other ratios such as 1:1 and 1:5 (Z:E) was not attractive to males. The attractiveness of Z:11-16:OH will be tested in near future.

약물치료명령은 성범죄자의 성적 환상 및 심각한 성범죄자의 충동을 약물을 이용하여 통제하는 치료 방법으로 그 도입이 확산되는 추세를 보인다. 우리나라 역시 2010년 7월에 성폭력범죄자의 성충동 약물치료에 관한 법률을 제정하였다. 성 범죄자에 대한 약물치료는 물리적 거세와 구분하여 화학적 거세라는 용어로 대체 사용된다. 화학적 거세는 이는 아동성범죄자를 포함한 상습적인 성범죄자 들에 대하여 성 충동 및 성적 호르몬(테스토스테른)을 감소시켜 성범죄를 예방 시키려는 의학적인 제재방법이다. 화학적 치료, 또는 의학적 치료라고도 한다. 화학적 거세를 위한 약물로는 남성호르몬을 억제시키는 시프로테론, 데포 프로 베라, 메드락시프로제스테른 등을 사용하며, 이들 약물은 남성의 섹스 충동, 강 박적인 성적 환상 및 성발기력을 감소시키는 효과가 있다. 약물치료를 받은 범 죄자들에 의한 평균 재범률은 5% 미만으로 나타났다. 그러나 성범죄자에 대한 화학적 거세가 대상자의 인권을 침해하며, 이중처벌이며, 부작용이 심각하다는 등의 지적이 있다. 또한 화학적 거세가 필요한 의학적 진단기준이 모호하다는 지적도 제기된다. 그러나 이러한 논쟁에 대하여 본 논문은 다음과 같은 입장이 다. 첫째, 약물치료의 부작용은 대상자의 생명에 지장을 줄 만큼 중대하지 않으 며, 그 부작용 역시 매우 일시적이라는 점이다. 치료를 중단할 경우 성적 능력도 회복된다. 그러나 성범죄 피해자의 신체적, 정신적, 경제적 피해는 평생 동안 지 속된다는 점에서 극단적인 성적 충동을 억제하지 못하는 범죄자에게 약물치료는 필요하다고 본다. 둘째, 화학적 거세의 효과는 매우 높아 성범죄의 재범률은 5%로 떨어진다. 이는 성범죄로부터 잠재적인 피해자, 그리고 지역사회를 보호 한다는 의미가 있다. 셋째, 의학적 치료명령은 이중처분이 아니라 보안처분으로서의 성격을 가진다. 법원 역시 같은 태도를 갖고 있다. 즉 치료명령은 현재 치료감호법 및 보호관찰등에 관한 법률에 따른 치료명령 또는 보호관찰 등과 같이 보안처분으로 볼 수 있다. 넷째, 의학적 치료명령 시 성범죄자의 자발적 동의가 반드시 필요하다고 볼 수 없다. 왜냐하면 앞에서와 같이 의학적 치료명령은 보안처분의 일종이므로 대상자의 동의가 필요하다고 볼 수 없으며, 의학적, 범행의 상습성 등을 고려하여 결정할 사안이라고 본다. 이에 대하여도 법원은 같은 입장으로 확인된다. 다섯째, 의학적 치료명령을 결정함에 있어 검찰 또는 법원 은 의학적인 소견이 주요한 판단근거로 활용하는 바, 보다 정교한 의학적 진단 규정이 마련되어야 한다. 이는 의사의 윤리적인 갈등을 최소화하는 데에도 중요 하다.

목적 : 본 연구는 MVPT-3(Motor Free Visual Perception Test-3)을 사용하여 나이, 성별, 교육 수준이 노인의 시지각 능력에 영향을 주는 요인을 알아보고, MVPT-3의 한국형 표준화작업을 위한 기초자료를 제공하기 위해 시행되었다. 연구방법 : 연구 대상은 G시에서 실시한 건강 검진을 위해 보건소에 방문한 뇌손상 경험이 없거나 치매 진단을 받지 않은 60 세 이상 노인 72명이였으며, 자료 수집은 면접조사와 MVPT-3을 사용하여 대상자의 시지각 능력을 평가하였다. 결과 : 연구결과 MVPT-3의 평균값은 남자가 28.74±7.79, 여자가 26.18±6.99로 남자가 여자보다 높았으나 통계적으로 유의하지는 않았다. 연령 간 차이를 보면 MVPT-3의 총점은 65세 미만이 29.54±7.94, 65세 이상은 26.32±7.01로 65세 미만에서 높은 점수를 보였고, MVPT-3의 기타 항목별 점수는 65세 미만에서 모두 높은 점수를 보였다. 항목별 점수는 시각적 완성(visual closure1)에서만 통계적으로 유의한 차이를 보였다. 교육 수준별로 살펴보면 초졸인 경우 MVPT-3의 평균값이 23.91±6.31이었으며, 중졸이상에서는 평균이 30.84±7.01이었다. 학력이 높을수록 점수가 높았으며 통계적으로 유의한 값을 보였다(p<.05). MVPT-3의 총점과 하위항목 간 점수별 상관관계 분석 결과 전정배경(figure ground)을 제외하고 모든 항목에서 통계적으로 유의한 상관관계가 나타났다. 결론 : MVPT-3을 이용하여 노인의 시-지각 능력에 영향을 주는 요인을 알아본 결과 성별에 따라서는 차이가 없고 연령과 교육수준에 따라 차이가 있었다. 이는 시지각 관련 평가를 수행하는데 있어 성별, 나이, 교육의 영향을 이해하고, MVPT-3의 표준화 작업 이전의 기초자료를 제공하는데 의미가 있을 것이다. 앞으로의 연구에서는 연령 범위를 좀 더 세분화하고 연구 대상자수를 확대하며 정상 노인과 환자군으로 나누어 비교해야 할 것이다.

이 논문은 예이츠부부가 결혼 초기에 실시했던 자동기술의 상당부분이 매우 개인적이며 성적인 내용으로 이루어져 있는 점에 주목하면서, 그러한 성적인 내용들이 자동기술에 어떻게 표현되어 있으며, 그것들은 자동기술에 표현되어 있는 철학적이고 종교적인 내용들과 어떤 관련성을 갖고 있는가, 또한 자동기술이 『비전』으로 엮어지는 과정에서 그러한 사적이고 성적인 내용들이 감추어지게 된 이유는 무엇이며, 그것들은 예이츠의 삶과 시에 어떤 영향을 주게 되는가에 대해 살피고 있다.

반복되는 아동 성폭력 범죄에 대한 강경대응 정책에도 불구하고 성폭력 전과자에 의한 재범이 계속 발생하고 있어 기존 지역사회 교정 및 범죄자 감독·처우에 대한 새로운 패러다임 변화가 요구되고 있다. 본 연구에서는 사회 안전 (public safety)과 범죄자 치료(offender treatment)라는 교정의 두 가지 목적을 새롭게 조명하면서 상이한 특징을 갖고 있는 두 교정모델을 소개하고 지역사회에서 두 모델을 활용할 수 있는 방안을 논의하고자 한다. 본 글은 먼저 성폭력범 죄자에 대한 지도․감독 틀로써 “지역사회 보호"(community protection)모델과 "공중보건”(public health)모델 두 가지를 설명한다. 그리고 위치추적 전자감시 제도 이전과 이후의 성폭력범죄자 특성을 비교함으로써 강제적으로 모든 가석방 성폭력범죄자에게 위치추적 전자감시제도가 부과됨으로써 어떤 범죄자 특성 변화가 초래되었는지 간략히 살펴본다. 즉, 지역사회 보호모델의 하나로 여겨지는 전자감시제도가 2008년 9월 1일 이후 의무적으로 부과됨으로써 가석방 선별 단계에서 실제 더 고위험의 성범죄자가 프로그램 대상자로 선정되었는지, 혹은 비공식적 기제를 통해 위험하지 않은 특정 범죄자에게도 전자감시가 부과됨으로써 의도하지 않은 결과가 초래되었는지, 위치추적 대상자의 기본 특징을 살펴 교정모델 적용 필요성을 새롭게 고찰해보는 것이다. 빈도분석 결과, 전자감시 이후 성폭력범죄자가 연령, 교육수준, 범죄경력, 혼인 면에서 전자감시 이전 성 폭력범죄자와 구분되는 특징을 갖고 있는 것으로 나타났다. 그리고 두 모델의 우위를 단순비교하기 보다는 사회보호 모델 이외에도 공중보건 모델 적용 필요성이 큰 것으로 나타났다. 사회보호 모델에 속하는 전자감시 제도가 간접적으로 어떠한 범죄자 특성을 초래했는지 살펴보고 향후 지역사회 교정모델이 성폭력 범죄자 보호관찰에 어떻게 적용되어야 하는지 그 방향을 검토한다.