신선한 울릉도 민들레 뿌리를 70% ethanol로 침지 추출하여 얻어진 추출물을n-hexane, EtOAc, n-BuOH로 순차용매 분획하였다. 이중 상대적으로 높은 pancreatic lipase 저해활성을 나타낸 EtOAc 분획에 대해 Toyopearl 및 겔을 활용한 column chromatography를 수행하여 5종의 페놀성 화합물을 분리하였다. 각 화합물의 화학구조는 NMR 스펙트럼 데이터 해석 및 표품과의 HPLC 직접 비교를 통

Several presowing seed treatments have been used to facilitate the seed germination and seedling emergence. The study was performed to design a model of presowing seed treatments in Taraxacum platycarpum. Germination and seedling growth responses to temperature, drying, chilling, GA3 and priming were investigated to know the optimum germination conditions and presowing treatment. The optimum temperature condition for germination and seedling growth were 15~20℃ at dark condition. The seeds exposed to infra red light, however, did not germinate. The moisture content of seeds dried at 35℃ for 3.5 hours was returned to the level of unimbibed seeds. The germination of seeds dried under dark condition was better than those dried under infra red light. Prechilling treatment imposed for 6 weeks showed higher germination rate than GA3 treatment or priming. In field test, the highest field emergence was observed in the seeds dried after prechilled for 6 weeks and followed by drying followed by GA3 〉 low temperature 〉 KNO3 priming. Imbibition with 0.1 mM GA3 solution for 2 hours then drying at 35 for 3.5 hour under dark condition was effective to the practical presowing treatment for Taraxacum seeds.

The propose of this study was to investigate the antioxidant activity of 80% ethanol extracts and various solvent fractions of dandelion (Taraxacmn officinale) leaves and roots, Total phenolics and phenolic acid contents were also examined. The total phenol content of leaves and roots were and respectively. Eight phenolic acids were separated by GC, among which caffeic acid (113.7 mg%)and m-coumaric acid (152.6 mg) were the dominant phenolic acids in leaves and roots, respectively. Amongst solvent functions of leaves and roots, the ethyl acetate fraction showed the strongest radical scavenging activity. A strong correlation was found between total phenol content and electron-donating ability, and ABTS radical scavenging activity showed a similar trend as electron-donating ability. Hydroxyl-radical-scavenging activity and lipid peroxidation were significantly higher in the ethyl acetate fraction than other factions. In particular, the SOD-like activity was highest (43.6%) in the ethyl acetate fraction of dandelion leaves, and was higher than that of trolox. Thus, the ethyl acetate fraction of dandelion leaves exhibited significant phenol content, antioxidant activity, and free-radical-scavenging effects.

Some studies of dandelion have been carried out on bioactivities, however, no comparative analysis on antioxidant and cytotoxic activities in the Korean dandelion (T. coreanum NAKAI.; KD) and dandelion (T. officinale WEB.) have been reported. In this study, the extracts of KD and dandelion analyzed relative phenolic contents and free radical scavenging, antioxidant enzyme and cytotoxic activities. The extract of Korean dandelion leaves (KDL) exhibited a higher phenolics content (368±11.5 mg/l00 g) and a strong free radical scavenging activity (RC50value;87.89 ug/ml) than other parts and BHT (120.12 ug/ml), synthetic antioxidant. At ascorbate peroxidase (APX) activity, the dandelion root (DR) had a greater (38.8 U/mg protein) antioxidant enzyme compare to the dandelion leaves (12.2 U/mg protein). The catalase (CAT) and superoxide dismutase (SOD) followed higher enzyme activity in Korean dandelion root (KDR) than other parts. In a cytotoxic activity against human cancer cell, the extracts of KDR was found to be active against Calu-6, HCT-116, and SNU-601 cell lines, with IC50 values of 522.34, 532.74 and 614.85 ug/ml, respectively. These results suggest that KD and dandelion would be an alternative antioxidant source, based on natural plant resources.

한국의 전통적인 민간 약재로 사용되어져온 흰민들레의 잎절편체로 부터 기관형성을 통한 재분화를 시도하였다. 잎 절편체를 BA (0-4mg/L)와 2,4-D (0-1mg/L)가 혼합 첨가된 MS 기본 배지에 치상하여 배양한 결과, 배양 3주후부터 신초가 발생되었다. 신초 분화율은 2mg/L BA 첨가된 조건에서 가장 높았으며, 배양 4주째 4mg/L BA 첨가된 조건에서는 캘러스가 유기되었다. 잎령에 따른 부정아 형성율, 발생빈도, 건량, 생중량을 조사한 결과, 발아 후 7주된 잎 절편체에서 평균 11.5개의 신초를 형성하였다. 유도된 신초는 0.5mg/L NAA가 첨가된 배지에서 발근되었으며, 화분에 옮겨 완전한 식물체로 재분화 되었다.