서식지에 따른 토양 절지동물 풍부도 및 딱정벌레 다양성 연구를 위해 세 종류의 시료(낙엽층과 토양 혼합, 낙엽층, 토양 시료)를 확보 후 체 털어잡기 하였다. 2023년 6월부터 8월까지 강원도와 충청북도에서 혼합 시료, 낙엽층 시료, 토양 시료 각각 25개씩 총 75개의 시료를 확보하였 다. 딱정벌레목 다양성을 확인한 결과, 세 가지 시료 중에서 혼합 시료에서 가장 높은 풍부도, 종 부유도, Shannon 지수가 나타났다. 이 논문에서 는 체털어잡기의 생태학적 활용 가능성에 대해 논의해 보았다. 토양성 절지동물의 채집과 시료 종류 사이의 연구가 지속적으로 이루어진다면, 최 종적으로 토양생태계 연구의 발전과 이해를 높일 수 있을 것으로 기대된다.

애기뿔소똥구리 (Copris tripartitus)는 배설물 분해를 통해 환경정화와 생태계 균형에 기여하는 것으로 알려져 있다. 그러나 무분별한 농약 사용과 서식지 파괴로 인해 개체수가 감소되고 있어 2017년 환경부에서 애기뿔소똥 구리를 멸종위기 야생생물 Ⅱ급으로 지정하였다. 애기뿔소똥구리의 미토콘드리아 유전자를 활용한 선행연구가 발표되었지만 유전자원 확보는 여전히 미비한 실정이다. 이번 연구는 애기뿔소똥구리의 성장, 면역 및 생식과 관련된 유전 정보를 확보하기 위해 Illumina HiSeq 4000 platform을 활용하여 전사체 분석을 실시하였다. Illumina HiSeq 4000을 통해 확보된 전사체 데이터를 Trinity 프로 그램을 통해 de novo assembly를 진행하여 contigs를 생성하였다. 생성된 contigs를 TGICL 프로그램을 통해 clustering 하여 unigenes을 확보하였다. 이후 확보된 unigenes는 PANM DB 및 Swiss-Prot, KOG, InterProScan, GO, KEGG를 기반으로 한 BLASTx를 사용하여 annotation을 진행하였다. 25,106개의 unigene 중에서 23,289개가 PANM DB에 annotation 되었으며, Swiss-Prot, InterProScan에서는 각각 19,660개, 13,545개의 unigene이 annotation 되었다. KOG 분석에서는 ‘general function prediction only’ 범주에서 높은 비율로 나타났으며, GO 분석에서는 ‘Molecular Function’ 카테고리에서 가장 많이 annotation 되었다. KEGG 분석을 통해서는 ‘Environmental information processing’ 항목이 높은 발현을 보였다. 이번 연구를 통해 확보된 기능적 데이터는 야생에서의 보존 계획을 수립하는 데 있어 기초 자료로써 활용될 수 있을 것으로 사료된다.

Lytic polysaccharide monooxygenases (LPMOs) catalyze the oxidative cleavage of glycosidic bonds in crystalline polysaccharides including chitin and cellulose. The recent discovery of LPMO family proteins in many insect species suggests that they presumably play a role in chitin degradation in the cuticle/exoskeleton, tracheae and peritrophic matrix during insect development. Insect LPMOs belong to auxiliary activity family 15 (AA15/LPMO15) and have been classified into at least four groups based on phylogenetic analysis. In this study, we identified and investigated the physiological functions of group I LPMO15 (MaLPMO15-1 and PhLPMO15-1) in two longhorn beetle species, Monochamus alternatus and Psacothea hilaris. In both species, depletion of LPMO15-1 transcripts by RNAi resulted in a lethal pupal-adult molting defect. The insects were unable to shed their old pupal cuticle and died entrapped in their exuviae. Furthermore, TEM analysis revealed a failure of degradation of the chitinous procuticle layer of their old cuticle, retaining intact horizontal laminae and vertical pore canals containing perpendicularly oriented chitin fibers (pore canal fiber, PCF) in their core. These results indicate that MaLPMO15-1 and PhLPMO15-1 are required for turnover of the chitinous old cuticle, which is critical for insect molting.

본 연구는 장수풍뎅이(Allomyrina dichotoma) 유충을 가수분해한 후 발생한 가수분해물 시료의 항비만 효능에 관한 결과이다. 먼저 3T3-L1 세포에 대한 시료의 독성을 테스트하였다. 시료 F10, P10, P30은 세포에 62.5ug/ml 처리 시 세포 생존율은 ~87.5% 정도로 나타났으며 F30의 경우 세포가 모두 건강하게 성장하는 것을 알 수 있었다. 다음으로 지질합성 관여 단백질인 SREBP-1과 FAS에 대한 발현 억제 실험을 진행하였다. 시료 F10과 F30을 처리 하였을 때 SREBP-1과 FAS의 발현을 가장 잘 억제한다는 것을 알 수 있었다. 3T3-L1 지방세포 분화 과정을 Oil Red O staining 방법으로 염색 후 관찰을 진행하였다. 컨트롤과 비교하여 시료 F10, F30, P10과 P30을 처리한 지방 세포들은 모두 세포 분화 억제가 관찰되었으며 특히, 시료 F10에서 지방세포 분화 억제가 활발히 발생한다는 것을 알 수 있었다. 위 결과를 종합해 보면, 시료 F10과 F30을 혼합하여 사용한다면 반려동물에 대하여 효과 있는 항비만 사료를 개발할 수 있으리라 판단된다.

Nicrophorus Fabricius, 1775, commonly known as necrophagous beetle, is associated with vertebrate carrion. Up to date, the genus consists of 72 species worldwide. In recent years, various phylogenetic studies explored on the evolution and relationship of the species in the genus. However, morphological and molecular phylogenetic studies produced conflicting results, continuing the problem over whether Nicrophrous quadraticollis is monotypic or not. The present study is to report the complete mitochondrial genome (mitogenome) of N. quadraticollis that was sequenced in prior to a systematic research of Silphinae. It was 17,747bp in length and comprised 12 protein-coding genes(PCGs), 2 rRNA Genes, 22 tRNA Genes and one non-coding region. The nucleotide composition is 40.9% for A, 36.7% for T, 9.0% for G and 13.4% for C.

The jewel beetle, Trachy dilaticeps Gebhardt, 1928 has been known to occur in Japan, China, Taiwan, and Vietnam hitherto. This species is typically distributed in Oriental region. In the case of Palaearctic region, it has only been documented in Japan (Kyushu). In this study, we report T. dilaticeps for the first time from Korea, along with its diagnostic characters. Ecological and distributional information is also provided herein.

The genus Stenotothorax is reported for the first time in the Korean Peninsula. While working on Korean Aphodiinae, we identified Stenotothorax hibernalis (Nakane & Tsukamoto) from the Goseong-gun, Gangwon Province. This species was found in the northern region of South Korea. A key to Aphodiini genera in Korea, habitus photographs, and illustrations of diagnostic characters are provided to facilitate identification.

Insect cuticular extracellular matrices (ECM) including the eggshell and exoskeleton play vital roles in protecting them from natural environmental stresses. However, these chitinous ECMs must be degraded at least in part during embryonic and post-embyonic molting periods to accommodate continuous growth all the way to the adult stage. In this study we investigated the functions of groups I and II chitinases, TcCHT5 and TcCHT10, in turnover of the eggshell and cuticle in Tribolium castaneum. RNAi and TEM analyses revealed that TcCHT10 is required for digestion of chitin in the serosal cuticle for embryo hatching as well as in the old cuticle during post-embryonic molts including larval-pupal and pupal-adult metamorphosis. However, although TcCHT5 is apparently involved in these vital physiological events, TcCHT10 could substitute for TcCHT5 except during the pupal-adult molting when both enzymes are indispensable to degrade chitin in the old pupal cuticle.

우리는 길앞잡이(Cicindela chinensis)의 장에서 다양한 공생 미생물들을 분리하였다. 그중 다양한 곰팡이 성장 을 억제하는 세균을 동정하였고 “Ch-1”이라 명명하였다. 우리는 Ch-1 균주를 사용하여 10종의 식물 병원성 곰팡 이와 2종 곤충 병원성 곰팡이의 생장 억제를 확인하였다. 또한 8종의 항생제에 대한 저항성을 확인하였다. 동시 에, 본 균주의 genomic sequence를 수행하였고 유전적, 생화학적, 생리적 특성을 조사하였다. Ch-1균주는 특허등 록과 친환경 미생물제제로 등록하였고 향후 생물학적 방제제로써 활용될 수 있을 것으로 판단한다.

The yellow mealworm beetle, Tenebrio molitor L. (Coleoptera: Tenebrionidae), has long been used as a key study organism in many fundamental researches, including biochemistry, physiology, and behavior. Lifespan and reproduction are two of the most important components of fitness in all insects, but it remains largely unexplored how these two traits are influenced by macronutrient intake in this beetle. In this study, we used the nutritional geometry framework to analyze the complex and interactive effects of dietary protein and carbohydrate intake on lifespan and reproductive performance in T. molitor beetle. Lifespan and the number of eggs laid throughout the lifetime were quantified from more than 2,000 individual beetles provided with one of 35 chemically defined diets representing a full combination of seven protein-to-carbohydrate ratios (P:C= 0:1, 1:5, 1:2, 1:1, 2:1, 5:1, or 1:0) and five protein plus carbohydrate concentrations (P+C=25.2, 33.6, 42, 50.4, or 58.8 %, dry mass). All measures of lifespan and egg production were expressed highly at high caloric intake, but they differed in the optimal P:C ratio where traits peaked. While lifespan was the longest at a moderately carbohydrate-biased P:C ratio of 1:1.36, the rate of egg production was maximized at a protein-biased P:C ratio of 1.75:1, suggesting a possible nutrient-mediated trade-off between lifespan and daily reproductive efforts in T. molitor beetles. Lifetime egg production was maximized at a P:C ratio of 1.31:1, which was still protein-biased but lower than that maximized egg production rate. Reproductive lifespan was the longest at a P:C ratio of 1:1.06. When given a food choice, T. molitor beetles preferred a P:C ratio of 1:1, which is closest to the ratio that enables T. molitor beetles to stay reproductively active as long as possible.

We analyzed comparatively the ground beetle communities of 1993 and 2023, with 30 year interval, in broadleaf and coniferous stands of the Gwangneung forest, a biosphere reserve in South Korea. 2,906 individuals (53 species, 38 genera, and 15 families) were collected from broadleaf stands and 1,782 individuals (36 species, 32 genera, and 14 families) from coniferous stands in 1993. The dominant species was Synuchus cycloderus in both stands. In 2023, 1,682 individuals (110 species, 78 genera, 21 families) were collected from broadleaf stands and 990 individuals (92 species, 67 genera, 22 families) from coniferous stands. In 2023, the dominant species were Eucarabus (Parhomopterus) sternbergi sternbergi in broadleaf stands and Coptolabrus jankowskii jankowskii in coniferous stands. Species richness (R'), species evenness (E'), species diversity (H'), and dominance (DI) were all higher than in broadleaf (R'=15.472, E'=0.336, H'=1.601, and DI=0.371) and coniferous (R'=13.756, E'=0.369, H'=1.324, and DI=0.326) forests in 2023.

경상북도에 위치한 산림유전자원보호구역내 2개 지역(영주지역의 신갈나무, 울진지역의 소나무)과 경기 포 천시에 위치한 생물권보전지역내 2개 지역(포천지역의 신갈나무와 소나무)에서 2016년부터 2017년까지 함정 트랩(Pitfall trap)을 매년 5회씩 5반복하여 각각 비교하였다. 그 결과 산림유전자원보호구역에서는 27과 106속 160종 2,665개체가 확인되었으며, 종풍부도는 20.28, 종균등도는 0.266, 종다양도는 1.355, 우점도는 0.265로 우점 종은 납작먼지벌레속 sp.2 (Synuchus sp.2)이였다. 또한, 생물권보존지역에서는 16과 49속 77종 4,834개체가 확 인되었으며, 종풍부도는 8.958, 종균등도는 0.301, 종다양도는 1.31, 우점도는 0.669로 우점종은 윤납작먼지벌레 (Synuchus nitidus)였다. 산림의 중요 지역 간에 지표성 딱정벌레의 군집을 비교함으로써 환경변화에 따른 산림 보존 프로그램을 수행하는데 유용한 기초 자료로 활용될 것이다.

최근 친환경 노지 감귤원을 중심으로 알락하늘소 피해가 증가하고 있지만 효과적인 예찰 방법이 없는 실정이 다. 본 연구에서는 알락하늘소 성충 예찰을 위하여 카이로몬을 선발하였고, 알락하늘소가 다발생(>100마리)한 과원 3개소에서 유인력을 평가하였다. 시험 결과, 집합페로몬 단독처리 시 알락하늘소 암컷 성충에 대한 유인효 과를 확인할 수 있었지만 소수 개체만 유인이 되었고, 카이로몬(Kairomone) 단독 처리 시 알락하늘소 성충 포획 수가 증가하는 것을 확인할 수 있었다. 집합페로몬과 카이로몬을 동시 처리 시 알락하늘소가 대량으로 유인되는 것을 확인할 수 있었다. 이는 알락하늘소가 종내신호물질(집합페로몬)을 인지할 때 서식지로서 적합한지 판단 의 기준으로 기주식물의 존재여부를 동시에 확인하기 떄문인 것으로 판단된다.

Insect cuticle is an extracellular matrix formed primarily from two different biopolymers, chitin and protein. During each molt cycle, a new cuticle is deposited simultaneously with degradation of the old cuticle by molting fluid cuticle degrading-enzymes, including epidermal chitinases (CHTs). Insect CHTs, belonging to family 18 glycosylhydrolase (GH18), have been classified into at least eleven subgroups based on phylogenetic analyses, and group I (CHT5) and group II (CHT10) epidermal CHTs present in molting fluid. In this study we report the physiological function of MaCHT5 and MaCHT10 in the Japanese pine sawyer, Monochamus alternatus. RNAi for either MaCHT5 or MaCHT10 resulted in larval-pupal and pupal-adult molting defects, in which the insects were unable to shed completely their old cuticle and died entrapped in their exuviae. Furthermore, TEM analysis revealed a failure of degradation of the old cuticle in both MaCHT5- and MaCHT10-deficient pharate adults. In the old pupal cuticle, the chitinous horizontal laminar and vertical pore canal essentially remained intact in the endocuticular layer. These results indicate that both CHTs are required for turnover of the chitinous old cuticle, which is critical for completion of insect molting. We also discuss the possible function of two spliced variants of MaCHT10, MaCHT10a and MaCHT10b.

A taxonomic study of Korean Psammodiini is present. Seven species [Rakovicius coreanus (Kim), Leiopsammodius japonicus (Harold), Psammodius flavolittoralis Kim, Psammodius hangangensis Kim, Psammodius sungshinarum Kim, Rhyssemus inscitus (Walker), Trichiorhyssemus asperulus (Waterhouse)] belonging to five genera are recognized based on morphological characters. A pictorial key, habitus photographs and illustrations of the diagnostic characters of Korean Psammodiini species are provided to facilitate identification.

Because of recent reports about phosphine resistance problem, development of effective fumigation method to control grain pests became very important. In this study, a chemical treatment, ethyl formate fumigant treatment, and a physical treatment, atmospheric control, were attempted as alternative solutions to this problem. In this study, for CA(Controlled atmosphere) treatment, 99.999% nitrogen was used to create a hypoxic condition with less than 5% oxygen, and for EF, the treatment concentration was 10 mg/L to 80 mg/L. As a result of the study, in CA single treatment, adult insects showed a mortality rate of less than 10% even after 2 weeks of treatment, and pupae and larvae showed a mortality rate 71% and 34%, but eggs showed a mortality rate of 100%. In EF single treatment, adults and larvae showed a 100% mortality rate at 80 mg/L, but eggs showed a 50% mortality rate and pupae were not affected. Considering the results, CA single treatment is not suitable for controlling Tribolium castaneum because of long treatment period, and in the case of EF single treatment, additional researches on longer treatment time is needed.

Beetles are one of the most effective transportation for phoretic chelicerata. They use various methods to attach themselves to their hosts, such as developing ventral sucker plates, grasping with their claws and chelicerae, or hiding beneath the elytra. Recently, we discovered new species of two genera: Antennoseius (Mesostigmata: Blattisociidae) and Gaeolaelaps (Mesostigmata: Laelapidae) which are hiding under the elytra of Carabidae hosts. In this study, digital images of the beetles, and mites, along with brief diagnostic information and line-drawing plates will be provided.

Lytic polysaccharide monooxygenases (LPMOs) catalyze the oxidative cleavage of glycosidic bonds in crystalline polysaccharides including chitin and cellulose. The recent discovery of LPMO family proteins in many insect species suggests that they presumably play a role in chitin degradation in the cuticle/exoskeleton, tracheae and peritrophic matrix during insect development. Insect LPMOs belong to auxiliary activity family 15 (AA15/LPMO15) and have been classified into at least four groups based on phylogenetic analysis. In this study, we identified, characterized and investigated the physiological functions of group I LPMO15 (MaLPMO15-1 and PhLPMO15-1) in two longhorn beetle species, Monochamus alternatus and Psacothea hilaris. In both species, depletion of LPMO15-1 transcripts in last instar larvae by RNAi had no effect on subsequent larval-pupal molting and the resulting pupae developed normally. However, adverse effects on their development were observed during the pupal-adult molting period. The pharate adults were unable to shed their old pupal cuticle and died entrapped in their exuviae probably due to a failure of degradation of the chitin in their old cuticle, which is critical for completion of the insect molting and continuous growth.

Insect eggshell and cuticle/exoskeleton play vital roles in protecting them from natural environmental stresses. However, these chitinous cuticular extracellular matrices must be degraded at least in part during embryo hatching and molting/ecdysis periods to accommodate continuous growth all the way to the adult stage. In this study, we investigated the functional importance of groups I and II chitinases, TcCHT5 and TcCHT10, in the turnover of chitinous cuticle during both embryonic and post-embryonic development in Tribolium castaneum. RNAi and TEM analyses revealed that TcCHT10 is required for digestion of chitin in the serosal cuticle for embryo hatching as well as in the old cuticle during post-embryonic molts including larval-pupal and pupal-adult metamorphosis. TcCHT10 appears to be able to substitute for TcCHT5 in all these vital physiological events except for the pupal-adult molting in which TcCHT5 is indispensable for complete digestion of chitin in the old pupal cuticle.