Industrial Motors diagnostic equipment is highly dependent on the automation system, so if there are defects in the automation equipment, it can only rely on the operator’s intuitive judgment.To help with intuitive judgment, Park’s Vactor Approach(PVA) represents the current signal as a pattern of circles, so it can tell if a fault occurs when the circle is distorted. However, the failure to judge the degree of distortion of the circle pattern is the basis of the fault, so it will face difficulties. In this paper, in order to compare the faults of PVA, the period of d-axis current of PVA pulsation was mastered, so that two phase differences occurred in the same signal source. Through experiments, it is confirmed that this is a 90 degree cross formation of PVA, which is convenient for judging from the vision that there is no fault, thus helping the operator to make intuitive judgment.

Monochamus alternatus (M. alternatus) and Monochamus saltuarius (M. saltuarius) are major vectors for Bursaphelenchus xylophilus in South Korea. When an adult, they are easily distinguishable by several morphological classification. However, it is difficult to identification between M. alternatus and M. saltuarius when they are larvae as they have very similar morphological characters. Thus, they are not easily distinguishable without expertise about Cerambycidae taxonomy. Furthermore, during epidemiological investigation, sometimes, adults or larvae would not be founded in death pine trees. For these reasons, in this experiment, we are able to identified between M. alternatus and M. saltuarius by mitochondrial 12S rRNA gene primers that are specific to 12S rRNA gene fragment of M. alternatus using larvae tissue and frass. Moreover, we had examined whether vectors that were already escaped from dead pine tree have Bursaphelenchus xylophilus or not by multiplex PCR using larva frass that was remained in dead pine tree.

In 2015, we reported a viral disease extremely fatal to Allomyrina dichotoma larvae spread in the majority of the larva-rearing farms in Korea. Currently, the virus-infected larva is diagnosed by PCR-based amplification but this requires laboratory equipment and agarose gel electrophoresis. Loop-mediated isothermal amplification (LAMP), a highly sensitive DNA amplification method, uses DNA polymerase isolated from Bacillus stearothemophilus and a set of six primers. It has great potential for field use because DNA is amplified under single temperature and the overall reaction completes in 30 min without laboratory equipments. Here, we report the development of on-site diagnosis method for Korean horn beetle larva infected by Allomyrina dichotoma Nudivirus.

경산 묘목단지는 대단위 과수종묘를 생산하고 있어 경쟁력 강화를 위하여 무독묘 보증 생산이 요구되고 있다. 특히 생산규모가 큰 사과종묘에 대한 빠르고 정확한 바이러스 진단이 시급하다. 본 연구에서는 사과바이러스 진단을 위하여 다량의 시료를 동시 분쇄할 수 있는 bead beater를 이용하였으며 분쇄 bead는 저가의 산업용 glass bead (0.4 mm 직경)를 일회용으로 채택하였다. RNA추출을 위하여서는 guanidine thiocyanate 용액이 Trizol 용액보다 효과적인 것으로 나타났으며 silica membrane tube의 이용으로 RNA추출 간편성을 높일 수 있었다. 사과바이러스는 RT-PCR에 의하여 검증하였다.

Root knot nematode species, such as Meloidogyne hapla, M. incognita, M. arenaria and M. javanica are economically most notorious nematode pests, causing serious damage to the various crops throughout world. In this study, DNA sequence analyses of the D1-D3 expansion segments of the 28S gene in the ribosomal DNA were conducted to characterize genetic variation of the four Meloidogyne species obtained from Korea and United States. PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism), SCAR (Sequence Characterized Amplified Region) marker and RAPD (Random Amplification of Polymorphic DNA) also were used to develop the methods for exact and rapid species identification. In the sequence analysis of the D1-D3 expansion segments, only a few nucleotide sequence variation were detected among M. incognita, M. arenaria, and M. javanica, except for M. hapla. The PCR-RFLP analysis that involves amplification of the mitochondrial COII and lrRNA region yielded one distinct amplicon for M. hapla at 500 bp, enabling us to distinguish M. hapla from M. incognita, M. arenaria, M. javanica reproduced by obligate mitotic parthenogenesis. SCAR markers successfully identified the four root knot nematode species tested. We are under development of RAPD primers specific to the three root knot nematodes found in Korea.

본 연구는 온실멜론의 양액재배에서 영양진단에 기초한 배양액관리를 하기 위해 유효한 수단으로 이용할 수 있고 신속하고 간편하며 정확한 즙액분석 방법(질산태질소의 정량방법, 인산정량방법, 즙액추출법, 추출비율, 마쇄시간 및 샘플채취 부위 등)들에 관한 비교검토를 하였다. 'Earl's Favorite 춘계를 1993년 1월 11일에 파종하여 본엽 3∼4매의 묘를 정식하여 비순환 방식으로 재배했다. 즙액분석법은 결핍이나 과잉증상이 외관적으로 나타나기 전에 신속하고 간이한 즙액분석법에 의해 물체의 영양상태를 진단하여 대응이 가능하다고 판단되었다. 즙액분석시 질산태질소의 분석에는 자외부 흡광도법, 인산분석에는 바나드몰리브덴산법이 추천되었고, 추출비율은 1：4로, 엽병의 마쇄시간은 60초, 그리고 채취부위는 일정부위로 하는 것이 바람직하다고 판단되었다. 그러나 샘플채취시간에 대해서는 채취시간에 따른 차이가 크기 때문에 앞으로 보다 상세한 검토가 요청되었다.

Background : Development of new real-time PCR diagnosis method for simultaneous diagnosis of Cylindrocarpon destructans and Fusarium solani, causative fungi of ginseng root rot disease. C. destructans and F. solani are known to be the major pathogens of ginseng root rot disease. Root rot caused by these pathogens is a disease that is difficult to control because the disease progresses slowly and it is difficult to diagnose early and even when symptoms of plant seeding are present, the disease is already spread in the roots. Diagnostic methods to detect the presence or absence of ginseng roots rot fungi in soil before ginseng cultivation are currently being used as a method for controlling. However, commercialized soil extraction kits and PCR diagnostics have cost, diagnostic time, and single diagnostic problems, and need to develop new diagnostic methods. Methods and Results : Primers and probes in the beta-tubulin 2 gene were designed for species-specific detection. In silico analysis, the detection rate of C. destructans was 100% and the detection rate of F. solani was 95%. The multiplex real time PCR optimization conditions including the internal control were established. The analytical sensitivity using positive samples was 10 copies/㎕ for C. destructans and 10 copies/㎕ for F. solani. As a result of performance comparison test with conventional PCR diagnosis methods, it was confirmed that the developed multiplex real time PCR method has the same or better performance in terms of sensitivity. In the developed soil extraction kit, the extraction time was reduced and the extracted DNA quality was improved, compared to the used soil extraction kit. Conclusion : From the above results, we expect that the developed C. destructans / F. solani multiplex real time PCR diagnosis method and soil extraction kit will be useful for real-time monitoring of ginseng root rot pathogenic fungi in the soil of ginseng cultivation area and diagnosis of suitability of ginseng cultivation area.

Pumping Station is amended ('15.1.6) a special law (hereinafter siteukbeop) on the safety management of the facility has been incorporated into kinds of facilities. This study aims to introduce siteukbeop drainage pumping stations as part of condition assessment techniques detailed guidelines established for drainage pumping stations being incorporated into the new facility as amended.