Slipchip offers advantages such as high-throughout, low cost, and simple operation, and therefore, it is one of the technologies with the greatest potential for high-throughput, single-cell, and single-molecule analyses. Slipchip devices have achieved remarkable advances over the past decades, with its simplified molecular diagnostics gaining particular attention, especially during the COVID-19 pandemic and in various infectious diseases scenarios. Medical testing based on nucleic acid amplification in the Slipchip has become a promising alternative simple and rapid diagnostic tool in field situations. Herein, we present a comprehensive review of Slipchip device advances in molecular diagnostics, highlighting its use in digital recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP), and polymerase chain reaction (PCR). Slipchip technology allows users to conduct reliable droplet transfers with high-throughput potential for single-cell and molecule analyses. This review explores the device’s versatility in miniaturized and rapid molecular diagnostics. A complete Slipchip device can be operated without special equipment or skilled handling, and provides high-throughput results in minimum settings. This review focuses on recent developments and Slipchip device challenges that need to be addressed for further advancements in microfluidics technology.

In the molecular cloud G33.92+0.11A, massive stars are forming sequentially in dense cores, probably due to interaction with accreted gas. Cold dense gas, which is likely the pristine gas of the cloud, is traced by DCN line and dust continuum emission. Clear chemical differences were observed in different source locations and for different velocity components in the same line of sight. Several distinct gas components coexist in the cloud: the pristine cold gas, the accreted dense gas, and warm turbulent gas, in addition to the star-forming dense clumps. Filaments of accreted gas occur in the northern part of the A1 and A5 clumps, and the velocity gradient along these features suggests that the gas is falling toward the cloud and may have triggered the most recent star formation. The large concentration of turbulent gas in the A2 clump seems to have formed mainly through disturbances from the outside.

Using ALMA observations of the 13CN and DCN lines in the massive star-forming region G33.92+0.11A, we investigate the CN/HCN abundance ratio, which serves as a tracer of photodissociation chemistry, over the whole observed region. Even considering the uncertainties in calculating the abundance ratio, we find high ratios (》1) in large parts of the source, especially in the outer regions of star-forming clumps A1, A2, and A5. Regions with high CN/HCN ratios coincide with the in infows of accreted gas suggested by Liu et al. (2015). We conclude that we found strong evidence for interaction between the dense gas clumps and the accreted ambient gas which may have sequentially triggered the star formation in these clumps.

The disadvantage of the current Red imported fire ants (RIFA) diagnostic is that it takes at least two days, because it is subjected to PCR amplification and DNA sequence identification process using an arthropod universal mitochondrial cytochrome oxidase I prime pair after genomic DNA extraction. In order to overcome this drawback, two RIFA specific genes were confirmed and used to develop techniques for identifying the species using various PCR methods. The conventional PCR method can be confirmed by the presence of amplified DNA, but additional time is required to confirm whether the PCR products are present or not. In contrast, SYBR green Real Time (RT) PCR or TaqMan probe RT PCR method has the advantage of confirming the results immediately after the reaction is completed. The new molecular diagnostic method has the advantage of shortening the time of two days, which is the biggest disadvantage of RIFA molecular diagnostic method, to 3 hours or less.

Recently, thanks to emerging ICT (Information and Communication Technology) such as IoT (Internet of Things), wireless telecommunication, and various sensor technologies, the concept of connected car has been highlighted in the automotive industry. In the connected car technology, one application is to diagnose and predict the car status in a real-time way based on gathered data. To this end, it is necessary to develop the diagnostics/prognostics algorithms for a specific part or component in a car. The results of diagnostics and prognostics could provide drivers with useful information used for advanced maintenance policy such as condition-based maintenance. In this study, we have reviewed the relevant previous research works before developing detailed algorithms.

The graphene oxides (GOs) were tested as a fluorescent quencher in the field of DNA-diagnostics. The various suspensions of GO nanoplates were prepared by changing the synthesis conditions. The suspensions were stable for at least 6 weeks by differing degrees of functionalization of various oxygen-containing groups of atoms. Depending on the properties of GO nanoplates, their fluorescent quenching abilities, which were determined by the amount of the tagged immobilized oligonucleotide, were also changed. GO suspension synthesized at 75 oC of reaction mixture showed the fluorescent quenching of 16.39 nmol/mg, which would be a potential substitution of molecular fluorescent quencher in test-systems for DNAdiagnostics.

We combine data from two all-sky surveys, the Swift/Burst Alert Telescope 22 Month Source Catalog and the AKARI Point Source Catalogue, in order to study the connection between the hard X-ray (> 10 keV) and infrared (IR) properties of local active galactic nuclei (AGN). We find two photometric diagnostics are useful for source classification: one is the X-ray luminosity vs. IR color diagram, in which type 1 radio-loud AGN are well isolated from other AGN. The second one uses the X-ray vs. IR color-color diagram as a redshift-independent indicator for identifying Compton-thick (CT) AGN. Importantly, CT AGN and starburst galaxies in composite systems can also be separated in this plane based upon their hard X-ray fluxes and dust temperatures. This diagram may be useful as a new indicator to classify objects in new surveys such as with WISE and NuSTAR.

There are at least three effects of the non-thermal particle bombardment on the solar atmosphere: (1) non-thermal ionization and excitation; (2) proton-hydrogen charge exchange; (3) impact line polarization. Due to the non-thermal ionization and excitation effects of electron bombardments in flares, Hα line is widely broadened and shows a strong central reversal. Significant enhancements at the line wings of Lyα and Lyβ are also predicted. In the case of proton bombardment, less strong broadening and no large central reversal are expected. However, due to proton-hydrogen charge exchange, the enhancements at the red wings of Lyα and especially of Lyβ lines at the early impulsive phase of flares are significant. Electron beam can also in some cases generates visible and UV continuum emission in white-light flares. However, at the onset phase, a negative 'black' flare may appear in several seconds, due to the increase of the H- opacity. The impact polarization of atomic lines can provide complementary information on the energetic particles, the energy transport and deposit in the solar chromosphere. New results of spectropolarimetric analysis for the major flare on July 23, 2002 are also given in the paper.

당뇨병 환자의 혈당치 측정을 위하여 다층젤라틴 필름으로 만들어진 진단막을 제조하였다. 플라즈마와 혈액 속의 글루코우즈의 농도 변화에 따른 최대확산속도를 측정하였다. 여러 가지 온도에서 3일, 2주, 4주 보관후 온도가 글루코우즈의 확산속도에 미치는 영향을 조사하였다. 다층 젤리탄 진단막의 안정성을 상대습도 80%에서 측정하였다.

Non-LTE calculations, with the non-thermal ionization effects included, indicated that for electron bombardment, the Hα line is widely broadened and shows a strong central reversal. Significant enhancements at the line wings of Lyα and Lyβ are also predicted at the beginning of the impulsive phase of flares. For the proton bombardment, no strong broadening and no large central reversal are expected. However, due to proton-hydrogen charge exchange, the enhancements at the red wings of Lyα and Lyβ lines at the early impulsive phase of flares are significant. Our results show that the electron beam can also in some cases generate visible and UV continuum emission in white-light flares. However, at the onset phase, a negative flare may appear within several seconds, due to the increase of the H- opacity. Another spectroscopic signature of energetic particles, i.e. the impact polarization of atomic lines, is also mentioned.

대향타겟트형 스파터기에서 BaO-l2Fe 복합타겟트를 사용하고 50% O2+Ar 스파터가스를 사용한 반응성 프라즈마를 스펙트로스포프법으로 검진하였다. 프라즈마의 스펙트럼은 Ba, Ba+, Fe, FeO, Fe+, Ar, Ar+, O, O+의 피크로 이루어져 있었으며 타겟트로 부터 멀어짐에 따라 이온의 상대강도는 중성원소의 그것에 비하여 더 감소하였다.

This paper is a part of the series on positron annihilation spectroscopy of two-phase diffuse gas-and-dust aggregates, such as interstellar medium and the young remnants of type II supernovae. The results obtained from prior studies were applied here to detect the relationship between the processes of the annihilation of the K-shell electrons and incident positrons, and the effects of these processes on the optical spectra of their respective atoms. Particular attention was paid to the Doppler broadening of their optical lines. The relationship between the atomic mass of the elements and the Doppler broadening, ΔλD (Å), of their emission lines as produced in these processes was established. This relationship is also illustrated for isotope sets of light elements, namely 3 6 7 7 9 10 11 2 3 3 4 4 5 5 He, Li, Li, Be, Be, B, and B. A direct correlation between the γ-line luminosity ( Eγ =1.022 MeV) and D Δλ (Å) was proved virtually. Qualitative estimates of the structure of such lines depending on the positron velocity distribution function, f(E), were made. The results are presented in tabular form and can be used to set up the objectives of further studies on active galactic nuclei and young remnants of type II supernovae.

The study investigates the validity of ‘subjecthood diagnostics’ in Korean proposed in the previous studies. Based on the studies on subjecthood diagnostics in Korean Single Subject Constructions (non-MSCs) and Multiple Subject Constructions (MSCs), the role of two subjecthood diagnostics―Honorific Agreement (HA) and Plural Copying (PC)―in these two constructions are examined through an empirical syntactic experiment. Seventy Korean native speakers participated in the experiment using acceptability judgment task designed to probe their intuition. The results show (i) that in the Non-MSCs, Honorific Agreement (HA) reliably seems to choose the subject (i.e., the only Nom-marked NP), whereas Plural Copying (PC) does not seem to pick out the subject in a less consistent manner; and (ii) that in the MSCs, neither Honorific Agreement (HA) nor Plural Copying (PC) seems to be a reliable subjecthood diagnostic. Possible reasons for these results are discussed.

본 연구의 목적은 교육용 치과 엑스선 발생장치를 설치 및 가동 중인 전국 치위생(학)과를 대상으로 방사선 작업종사자의 방사선 안전 관리 실태를 설문 조사하고 이를 체계적으로 추이 분석하여 교육용 치과 엑스선 발생장치의 안전한 이용 기반을 확립하기 위한 기초 자료로 제공하고자 하였다. 이를 위하여 교육용 치과 엑스선 발생장치의 방사선 안전 관리와 관계되는 원자력 관계 법령 및 국내 방사선 안전성 평가 및 규제 기술 개발 현황에 대한 용역 보고서를 참고하여 포괄적인 문헌 조사를 하였으며 이를 토대로 응답자의 일반적 특성, 방사선안전관리자의 위상, 방사선 안전관리 현황과 지식 및 의식 수준에 대한 설문지를 작성하였다. 본 연구는 국내 교육기관 내 방사선안전관리자와 교육용 치과 엑스선 발생장치를 운전 가능한 학사 및 실습 조교, 전임 교원 224명을 대상으로 설문 조사를 시행하여 설문 응답 누락 및 불충분한 응답을 제외한 95부를 회수하여 분석 자료로 이용하였다. 분석 방법은 일반적 특성 및 방사선안전관리자의 위상은 빈도와 백분율을 구하였으며 방사선 안전 관리 현황과 지식 및 의식 수준은 빈도 분석과 설문 문항별 연관성 분석을 위한 χ2 검정(chi-square test)과 수준간 연관성을 구하기 위한 피어슨 상관분석(Pearson correlation analysis)을 하였다. 결과적으로 교육용 치과 엑스선 발생장치의 운영은 대부분 대학 이상의 사회적 교육 수준이 높고 치위생학을 전공한 20대에서 40대의 여성이 담당하고 있었으며 남성과 비교하여 방사선 안전 관리에 대한 의식 수준이 높게 나타났으며 통계적으로 유의한 선형적 관계를 보였다(χ2 >5, 0.1<r<0.3, p<0.05). 또한 국가 기술 면허의 보유는 치과위생사가 55.4%로 가장 많았으나 방사성동위원소 취급자 일반 면허자(28.2%) 또는 방사선사 면허자(16.4%)와 비교하여 방사선 안전 관리에 대한 의식 및 지식 수준이 낮게 나타났으며 통계적으로 유의하게 뚜렷한 선형적 관계를 보였다(χ2 >5, 0.3<r<0.7, p<0.01). 특히 업무 경력이 증가할수록 방사선 안전 관리에 대한 지식은 높게 나타났으나 의식 수준은 감소하는 것으로 분석되었다(χ2 >5, 0.3<r<0.7, p<0.01). 따라서 방사선 안전 관리에 대한 전문적 지식 체계의 확립과 국내 외 방사선 안전 관리 체계를 포괄적으로 이해할 수 있는 제도화된 개선책 마련이 시급할 것으로 판단되었으며 교육용 치과 엑스선 발생장치의 사용에 따른 방사선작업종사자와 주변 일반인의 방사선 위해로부터 방어를 위한 체계적인 방사선 안전 관리 지침서의 개발이 필요하다고 판단되었다.

기상예보모형 중 장기예측에 널리 사용되는 CGM모의결과를 이용하여 확률론적 불확실성 해석기법의 적용을 통해 유역단위로 관리되는 국내 수자원 운영에 대한 활용 가능성을 분석하였다. 연구된 기법은 GCM 모의값이 관측값의 크고 작음을 얼마나 잘 구분하는지를 확률적으로 분석하는 방법으로 Kolmogorov-Smirnov 검정을 사용한다. GCM 모의값으로는 ECMWF에서 AMIP-II 형태로 모의한 결과로부터 표면강수량을 추출하여 사용하였으며, 관측값은 국내