본 연구는 식물공장에서 미나리(Oenanthe javanica DC.) 를 초장별(20cm, 30cm, 40cm, 50cm)로 재배할 경우 기능성 물질과 영양학적 가치가 유지되는지를 평가하기 위해 수행되 었다. 미나리는 항산화 효과, 항염증, 간 보호 등 다양한 생리 활성을 갖춘 기능성 채소로, 기존 재배 방식의 한계를 극복하 고 연중 안정적인 생산이 가능한 식물공장 재배 가능성을 확 인하는 것이 본 연구의 목적이다. 연구 결과, 초장 30cm에서 칼륨(K)과 칼슘(Ca) 함량이 가장 높았으며, 비타민 C 함량 또 한 1,165.38mg·kg-1으로 가장 높았다. 총 폴리페놀 함량은 초 장 20-40cm에서 유사한 수준을 유지하였으며, 초장 50cm 에서 감소하는 경향을 보였다. 기능성 물질인 Quercetin, Apigenin, Isorhamnetin 함량은 초장에 따른 변동 폭이 크지 않아, 단기 재배된 초장이 짧은 미나리도 충분한 기능성 물질 을 함유하고 있음을 확인하였다. 식이섬유 함량 또한 모든 초 장에서 일정 수준(300g·kg-1 이상)을 유지하여 건강 기능성 채소로 활용 가능성이 높았다. 본 연구 결과를 종합하면, 식물 공장에서 초장 30cm로 재배된 미나리는 높은 영양 가치와 기 능성을 유지할 수 있으며, 기존 노지재배 방식과 비교해 연중 균일한 생산이 가능하여 산업적 활용 가치가 클 것으로 판단 된다.
[ ] alloys with Al, B or Nb were prepared by an advanced consolidation process that combined mechanical alloying with pulse discharge sintering (complex forming) to improve the mechanical properties. Their microstructure and mechanical properties were investigated. The alloys fabricated by complex forming method showed very fine microstructure when compared with the sample sintered from commercial powders. Alloys made from powders milled in Ar gas had fewer silica or alumina phases as compared to their counterparts sintered from powders milled in air. In densification of the sintered body, addition of B was more effective than Al or Nb. Both Victors hardness and tensile test indicated that the alloy fabricated by the complex forming method showed better properties than the sample sintered from commercial powders. The Al added alloy sintered from the powders milled in air had the superior mechanical properties due to the suppression of and formation of fine particles.
Background: In the present study, we established an HPLC (high performance liquid chromatography)-analysis method for the determination of marker compounds as a part of the material standardization for the development of health-functional foods from Salvia plebeia R. Br. extract.
Methods and Results: The quantitative determination method of hispidulin as a marker compound was optimized by HPLC analysis using a YMC hydrosphere C18 column with a gradient elution system. This method was validated using specificity, linearity, accuracy, and precision tests. It showed a high linearity in the calibration curve with a coefficient of correlation (r2) of 0.999995. The method was fully validated, and was sensitive, with the limit of detection (LOD) at 0.09 ㎍• ㎖−1 and limit of quantification (LOQ) at 0.27 ㎍• ㎖−1. The relative standard deviation (RSD) values of the data from intra- and inter-day precision were 0.05 - 0.22% and 0.32 - 0.42%, respectively, and the intra- and inter-day accuracy of hispidulin were 99.5 - 102.3% and 98.8 - 101.5%, respectively. The average content of hispidulin in Salvia plebeia R. Br. extract was 3.945 ㎎• g−1 (0.39%).
Conclusions: These results suggest that the developed HPLC method is very efficient, and that it could contribute to the quality control of Salvia plebeia R. Br. extracts as a functional ingredient in health functional foods.