국내에서 오래 전부터 오미자와 칠해목은 약용식물로 이용해져 왔다. 오미자의 경우 생리활성 물질인 lignan을 통하여 여러가지 효능들이 연구를 통하여 알려졌으나, 칠해목의 경우 항염증과 관련된 연구가 진행되었을 뿐, 산화 스트레스와 관련된 연구는 미비한 실정이었다. 이에 본 연구에서는 오미자․칠해목의 추출혼합물을 이용하여, 과산화수소로 산화 스트레스가 유도된 SH-SY5Y 신경세포에서의 보호 효과를 알아보고자 하였다. 과산화수소의 처리 농도의 경우, 신경세포 독성 실험을 통하여 100 μM의 농도를 본 연구에 사용하였다. 또한, 오미자와 칠해목 추출물은 SH-SY5Y 신경세포에서 세포 독성이 없음을 실험을 통해 확인하였으며, 과산화수소를 이용한 신경세포 보호효과를 확인한 결과, 30% 에탄올 추출물 50 μg/mL의 농도에서 각각의 추출물에서 가장 높은 보호 효과를 확인할 수 있었다. 이에 오미자․칠해목 추출혼합물의 최적 효능 비율을 알아보기 위해서 각각의 추출물을 다양한 수율로써 6:4, 7:3, 8:2의 비율로 신경세포 보호 활성을 확인한 결과, 오미자와 칠해목 7:3의 비율과 50 μg/mL의 농도에서 가장 높은 신경세포 보호효과가 나왔으며, 이에 본 연구에서 오미자․칠해목 추출혼합물의 최적의 비율과 처리 농도로 사용하였다. Annexin V와 PI를 이용한 SH-SY5Y 신경 세포의 세포사멸을 확인해 본 결과에서도 오지마․칠해목 추출혼합물은 SH-SY5Y 신경세포의 세포사멸를 억제시키는 것을 확인할 수 있었다. 이에 본 연구에서는 오미자․칠해목 추출혼합물이 과산화수소로 유도된 산화적 스트레스에서 SH- SY5Y 신경세포에서 보호 효과가 있다는 것을 확인할 수 있었지만, 그에 대한 메카니즘에 대한 연구는 미비한 실정이다. 이에 차후 연구에서는 오미자․칠해목 추출혼합물의 산화 스트레스에 대한 정확한 기전을 파악하기 위하여 In vitro와 In-vivo에서의 후속적 연구가 필요하다고 사료된다.

Mitochondria is energy generating organelle. It synthesizes ATP, which is the essential energy source of many cellular processes. During producing energy, some redox centres leak electrons to oxygen and it is contributory to the reactive oxygen species. Besides, mitochondria have significant functions in metabolism, calcium homeostasis, and fatty acid oxidation. Also mitochondria has importance to the breakdown of the ovarian follicles and could be factor determining oocyte of quality adversely. Increasing evidence shows that the number of mitochondria affect oocyte of developmental competence and maturation detrimentally during aging. Oocyte is the mitochondria-rich cell and enable the organelle to have competence for fertilization and early embryonic development. Occurrence of blastomere depends on distribution change of mitochondria which present in the egg. Lonicera caerulea treatment inhibited ovarian mitochondrial oxidative damage by suppressing mitochondrial reactive oxygen species (mROS) generation, decreasing apoptosis, controlling disintegration of mitochondrial membrane potential and conserving respiratory chain complex activities. The purpose of this study is to identify if mouse accepting treatment with L. caerulea could counter age-induced sterility and ovarian mitochondrial OS in a model organism of ovarian ageing.

The citrus flavonoid hesperetin has various pharmacological actions, including antioxidant, anti-inflammatory, and anticancer activities. The purpose of this study is to confirm whether the treatment of hesperetin can protect the oocyte from in vitro aging. Porcine oocytes were matured in vitro for 44 h (control) and for an additional 24 h in the presence of 0, 1, 10, 100, and 250 μM hesperetin (aging, H-1, H-10, H-100 and H-250, respectively). This study investigated the effect of different concentration of hesperetin on maturation, and reactive oxygen species (ROS) level, apoptosis index, and the developmental capacity of aging porcine oocytes. In the results, the percentage of cleaved oocytes that reached to the blastocyst stage of H-100 group (37.9 ± 1.1%) was similar to control (38.1 ± 0.8%), and also significantly higher than other aging groups (23.2 ± 0.8%; H-1, 19.7 ± 1.3%; H-10, 26.7 ± 0.6%; and H-250, 18.4 ± 1.6%.)(p<0.05). The H-100 group was significantly decreased ROS activity, and increased the level of glutathione (GSH) and expression of the antioxidant genes (PRDX5, NFE2L, SOD1 and SOD2) compared to the aging group. The H-100 groups prevented aberrant spindle organization and chromosomal misalignment, blocked the decrease in the level of phosphorylated-p44/42 mitogen-activated protein kinase (MAPK), and increased the mRNA expression of cytoplasmic maturation factor genes (GDF9, CCNB1, BMP15 and MOS). Also, it was confirmed that the H-100 group expressed higher level of estrogen receptor than the aging group. Therefore, this result indicated that hesperetin is an effective agent to protect from the oxidative stress during in vitro aging of porcine oocytes.

곡물 저장중 해충과 미생물에 의한 피해가 증가하며, 이를 제어하기 위한 방법으로 이산화염소가스처리 방법을 제시하였고, 본 가스처리에 의한 살충기작을 증명하였다. 해충을 억제할 수 있는 최적의 가스 처리 조건과 가스 처리 후 곡물내의 이산화염소 잔류량과 곡물품질 등을 조사하여 피해 예측을 하였다. 본 가스 처리에 의해 화랑곡나방의 모든 생장 단계에서 효과를 보였으며, 특히 알과 성충 단계는 단시간 고농도 처리와 장시간 저농도 처리에서 높은 치사율였다. 한편, 낮은 농도에서 처리된 유충은 유충기간이 1.5 배가량 늘어나며, 번데기가 되지 못하거나 우화하지 못하는 것을 확인 하였다. 특히 유충의 섭식양이 급격히 감소하여 실크분비량이 거의 없을 정도로 활동량이 둔화되었다. 또한, 이산화염소처리가 화랑곡나방 암수 성충간의 페로몬 센싱에 교란을 유도할 수 있음을 확인 하였다. 본 연구 결과는 이산화염소가스처리를 통한 해충 방제 및 해충활동 제어 방법으로의 가능성을 제시한다.

다양한 식재료의 소독재로 사용되는 이산화염소(ClO2)는 이 물질이 갖는 높은 산화력에 기인된다. 이산화염소가 해충에 대한 방제 가능성이 제기되었고, 이에 대한 실증 시험은 이 물질의 살충기작을 증명하여 주었다. 그러나 이 물질의 살충 기작은 밝혀지지 않았다. 본 연구는 이산화염소의 훈증처리에 따라 곤충 체내에서 일어나는 산화적 스트레스를 항산화효소의 발현 유발에 따라 가능성을 찾았다. 다시 이산화염소를 처리하고 세포내에 존재하는 활성산소(reactive oxygen species: ROS)를 정량화한 결과 ROS의 급격한 증가를 관찰하였다. Vitamin E와 같은 항산화제를 이산화염소와 처리할 경우 살충력은 크게 낮아져 산화적 스트레스에 기인된 살충기작을 뒷받침하여 주었다. 이러한 ROS 유발은 다양한 생리현상을 일으키는 분자를 교란하여 곤충의 신경, 면역, 행동 및 발육 생리를 저해는 것으로 나타났다. 이러한 결과는 산화적 스트레스에 의해 살충효과를 일으키는 새로운 해충 방제제의 개발을 제시하고 있다.

This study was carried out to identify medicinal mushrooms with protective effects against oxidative stress in PC12 neuronal cell line, followed by evaluation of their antioxidant property. Extracts of medicinal mushrooms, including Ganoderma lucidum extract (GLE), antler-shaped Ganoderma lingzhi extract (AGLE), Hericium erinaceus extract (HEE), and Sanghuangporus baumii extract (SBE), were screened for cytotoxicity using MTT assay. None of the extracts up to 10 μg/ml concentration affected cell viability. These extracts were further checked for their protective effect against oxidative stress-induced reactive oxygen species (ROS) production. Exposure to 50 μM H₂O₂ induced ROS generation in PC12 cells, which was inhibited only by treatment with AGLE. In addition, inhibition of H₂O₂-induced ROS generation by AGLE was found to be in a dose-dependent manner (2.5, 5, and 10 μg/ml). Microscopic examination of DCF fluorescence for detection of ROS showed a similar pattern. Further, antioxidant activity of AGLE was determined by ABTS radical cation assay, and its IC50 was found to be 46.90±0.31 μg/ml. Taken together, these results suggest that AGLE may help to alleviate oxidative stress in PC12 neuronal cells.

Ultraviolet B (UVB) exposure is a risk factor for skin damage resulting in oxidative stress, inflammation, and cell death. The purpose of this study was to investigate the physicochemical properties of Platycodon grandiflorum (PG) to improve its biological activities using a three-step steaming process. We investigated the protective effects of PG and steamed PG extracts on human dermal fibroblasts (HDFs) against UVB radiation-induced oxidative stress and inflammation as well as the underlying mechanisms. The antioxidant potential of the PG extracts was evaluated by measuring the 2,2-diphenyl-1- picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) scavenging activity. ABTS and DPPH were shown by the 0, 30, and 70% ethanol extracts of 2S-PG and 3S-PG (IC50, 28~45 and 27~30 μg/mL, respectively). Treatment of UVB-irradiated cells with steamed PG (25~400 μg/mL) did not affect their viability. The streamed PG extract suppressed UVB-induced generation of reactive oxygen species (ROS). In addition, streamed PG extract reduced cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) protein expression in UVB-irradiated HDF, regulating nuclear factor (NF)-κB expression. These findings suggest that steamed PG extract may be potentially effective against inflammation associated with UVB-induced oxidation stress.

본 연구에서는 참죽나무 잎 추출물이 에탄올로 유도된 산화스트레스로부터 간세포를 보호하며, 항산화 효소 유전자 발현을 증가시킴으로써 항산화 활성을 보이는 것으로 사료된다. 또한, 참죽나무 잎에는 플라보노이드가 다량 함유되어 있으며, 특히 퀘르세틴 배당체인 qercetin 3-O-rhamnoside(quercitrin)는 건조중량 100 g당 1,456.0±12.5 mg으로 총 플라보놀 함량(1,874.5±15.5 mg)의 77.7%를 차지하여 참죽나무잎의 주요 플라보놀 성분으로 확인되었다. 결론적으로 참죽 나무 잎 추출물은 알코올에 의한 간 세포의 손상을 보호하고, 산화스트레스를 감소시켰으며, 이는 참죽나무 잎의 주요 플라보노이드인 퀘르세틴과 비교했을 때도 그 효과가 우수함을 확인하였다. 따라서 참죽나무 잎은 알코올에 의해 손상된 간을 보호하는 식품으로서 이용가치가 높을 것으로 기대된다.

Eighty pigs (40 pigs per farm aged 40 days old) that had been raised on two commercial pig farms A and B were used to evaluate oxidative stress status. The results from each farm were compared to investigate a relationship between pig performance and oxidative stress status. Pig performance on farm A was relatively better than that on farm B for the period of 3 years. The level of plasma total antioxidant status (TAS) of the pigs in group 1 (farm A) was significantly higher (p=0.045<0.05 ) than that of the pigs in group 2 (farm B). The level of plasma total oxidant status (TOS) and oxidative stress index (OSI) value of the pigs in group 2 were significantly higher (p= 0.045<0.05 and p=0.001<0.05) than those of the pigs in group 1 These results revealed that pig performance was associated inversely with oxidative stress status.

The seaweed Ecklonia cava, a brown algae abundant in JeJu Island, South Korea, has large amounts of the polyphenol compound phloroglucinol (PG, 1,3,5-trihydroxybenzene), which has been proposed to exert interesting biological properties including antioxidant and radioprotective effects against ionizing radiation-induced damages in various cells and tissues. To identify antioxidant and radioprotective effects of PG in skin tissues, we exposed mice to 8.5 Gy whole body irradiation (WBI) at day 6 after depilation with and without PG treatment. In PG treated cases, PG was applied twice, once at 17.8 hours before and then at the time of WBI. At 8 hours after WBI, a reduction in the formation of thiobarbituric acid-reactive substrates (TBARS) was observed in the PG treated group. Upon western blot analysis, PG treatment overexpressed the MnSOD, catalase, and GPx-1, although the difference was not significant. In parallel with the results of western blot analysis, the percentage of MnSOD-and catalase-positive cells was significantly increased at 8 and 24 hours after WBI, while no significant difference was observed over 48 hours in PG treated skins. Moreover, PG treatment increased the percentage of Ki-67 positive cells compared with that of irradiated only mice at 8 hours after WBI. Our results suggest that PG is effective at attenuating oxidative stress, and that the promotion of antioxidant enzymes such as MnSOD and catalase may be an important aspect for its radioprotection in skin.

Free radicals originate due to the radiolysis of cytoplasmic water with low “Linear Energy Transfer” (LET) radiations. Naringenin (Ng) is a natural antioxidative compound found in citrus fruits. This study revealed that Naringenin (Ng) reduced the radiation damage of critical organs by scavenging oxidative free radicals. In the study, Ng was orally administrated to rats daily for 7 consecutive days, prior to whole body exposure to gamma-rays. The scavenging efficacy was evaluated biochemically by measuring the concentration of cytotoxic byproducts and the activity of enzymes relevant to oxidative free radicals, after extracting the organs from the exposed rat. We observed increased levels of malondialdehyde (MDA) concentration, and decrease in the activities of superoxide dismutase (SOD) and catalase (CAT) in the exposed control group. However, pretreatment with Ng significantly reduced the MDA concentration, and increased the activities of SOD and CAT, as compared to the control group, due to the free radical scavenging by Ng. The results indicate that Ng administration prior to irradiation could protect critical organs from radiation damage.