2022년 1월부터 11월까지 부산지역에서 유통 중인 신선 농산물 210건을 대상으로 리스테리아균 분포 현황 및 병 원성 여부를 조사하였으며, 분리된 균주를 대상으로 혈청 형 및 유전자 지문분석을 통해 역학적 연관성을 확인하였 다. 조사 대상 신선농산물에서 총 40건의 리스테리아균이 검출되었으며, Listeria monocytogenes 등 4종의 리스테리 아균이 검출되었다. 이 중 L. innocua가 22건, L. monocytogenes 10건, L. grayi 6건, L. rocourtiae 2건으로 나타났으며, 이 중 식중독을 유발하는 L. monocytogenes균 은 팽이버섯에서만 검출되었다. L. monocytogenes의 병원 성을 유발하는 유전자인 iap, prfA, inlA, inlC, inlJ 및 hly 6종에 대한 분석 결과, 총 10개 균주 중 6개 균주에서 iap 등 6종의 병원성 유발 유전자가 검출되었으며, 4개 균주 에서 hly를 제외한 5종의 유전자가 검출되어 식중독 발생 잠재위험이 있음이 확인되었다. 지역 유통 식재료에 분포 하는 L. monocytogenes의 유전학적 유사도 및 오염원 추 이를 확인하기 위해 신선농산물에서 분리한 L. monocytogenes 10균주 및 2022년 부산지역 유통 가금류에 서 분리한 L. monocytogenes 2균주를 대상으로 혈청형 분 석 및 PFGE를 실시한 결과, 신선농산물 분리균주의 혈청 형은 1/2a 및 1/2b 두 가지 serotype으로 확인되었으며, 가 금류 분리균주는 모두 1/2a형으로 나타났다. 유전자지문 분석결과, 전체 균주의 유사도는 100-45.7%로 나타났고, 이 중 100% 상동성을 보인 균주들은 동일 생산농장 또는 동일지역 유래 팽이버섯에서 분리되어 오염원의 출처가 같음을 추측할 수 있었다. 신선농산물 분리균주와 유통 가금육 분리균주와의 유사도 확인 결과, 일부 팽이버섯 분 리균주와 가금육 분리균주의 유사도가 90.9-84.6%로 나타 났다. 농산물 및 축산물 생산시설간 오염원 이동 및 교차 가능성을 유추할 수 있었다.

Aligned with the recent shift in fruit consumption trends, there is a growing increase in the cultivation of these fruits. Thus, various types of small fruits, including blueberries, have recently been introduced and cultivated in Korea. According to the data, there has been a notable uptick in blueberry farming, in stark contrast to the decline in cultivation of black raspberries, black chokeberries, and blackcurrants. New varieties of blueberries continue to be introduced and cultivated, aligning with the recent consumption trends and are expected to be consistently cultivated in the future. Despite the decrease in the other berries, health benefits associated with all berries have ignited growing consumer interest, resulting in domestic and international market expansion. In conclusion, this research underscores the importance of identifying plant varieties that are well-suited to Korea's climatic conditions, validating efficient cultivation strategies, and establishing robust distribution networks to foster sustainable development in the berry industry.

청말·민국 시기 중국 지리교과서는 한국 관련 서술이 풍부하여 당시 중국인들이 한국을 어떻게 인식했는지 연구하는 데 유용한 공적 자료이 다. 한·중 양국은 수천 년 동안 정치, 경제, 문화 등 많은 방면에서 교류 했으며, 19세기 말 이전까지 한국은 중국 주도 하의 중화질서에서 가장 중요한 일원으로서 중국의 전통 지리 문헌에서 항상 ‘소중화’와 ‘예의지 방’으로 높은 명성을 누렸다. 그러나 20세기 들어 중국 지리교과서 속 한국의 이미지는 이전과는 확연히 달라졌다. 본 논문은 청말·민국 시기 중국의 교육 근대화 이후 편찬된 일련의 신식 지리교과서에 나타난 한국 의 개화기 및 일제강점기 관련 서술과 인식을 비교연구로 하였다. 19세 기 말 이후 한·중 양국 관계는 불평등에서 평등으로, 상하급에서 동반자 로 전환되었고 이에 따라 청말·민국시기 중국 지리교과서에 나타난 한국 의 이미지도 대체로 ‘소중화’에서 ‘후진국’으로, 그리고 다시 ‘동반자’로 바뀌는 과정을 거쳤다. 중국 지리교과서 속 한국의 이미지는 시기별로 중국이 직면한 여러 가지 현실적인 곤경에 영향을 받아 변화해 왔다. 대 중의 지리 지식과 타국 인식을 양성하는 주요 매체인 지리교과서에 대한 연구를 통해 당시 중국 정부와 교과서 편찬자가 자라나는 후속세대에게 한국을 어떻게 인식시키려고 했는지를 살펴보았다. 그리고 견문록, 신문 잡지 등 사적 자료를 연구 내용으로 한 기존의 선행 연구와 상호 검증과 보완의 관계를 형성할 수 있기를 기대한다.

This study aimed to investigate the dietary changes among adults living alone during the COVID-19 pandemic in the Republic of Korea. An online survey was conducted to examine dietary changes before (in 2019) and during (in 2021) the pandemic. The data from 337 adults living alone who responded to the survey were used for analysis. The proportion of the respondents reporting frequent food consumption at convenience stores (≥3 times/week) increased during the pandemic (p=0.024), and the proportion of those frequently eating ready-to-eat and ready-to-cook food (≥3 times/week) was more than doubled (p<0.001). Additionally, the proportion of those frequently consuming delivered food (≥3 times/week) increased by 2.5 times (p<0.001). In conclusion, the dietary habits of adults living alone changed significantly during the COVID-19 pandemic, which may have a negative impact on their health. Therefore, the development of customized nutrition management programs to improve the dietary habits of adults living alone during emergencies like a pandemic is deemed necessary. This study can serve as a foundation for understanding the dietary changes of adults living alone in prolonged crisis.

The grassland section of the greenhouse gas inventory has limitations due to a lack of review and verification of biomass compared to organic carbon in soil while grassland is considered one of the carbon storages in terrestrial ecosystems. Considering the situation at internal and external where the calculation of greenhouse gas inventory is being upgraded to a method with higher scientific accuracy, research on standards and methods for calculating carbon accumulation of grassland biomass is required. The purpose of this study was to identify international trends in the calculation method of the grassland biomass sector that meets the Tier 2 method and to conduct a review of variables applicable to the Republic of Korea. Identify the estimation methods and access levels for grassland biomass through the National Inventory Report in the United Nations Framework Convention on Climate Change and type the main implications derived from overseas cases. And, a field survey was conducted on 28 grasslands in the Republic of Korea to analyse the applicability of major issues. Four major international issues regarding grassland biomass were identified. 1) country-specific coefficients by land use; 2) calculations on woody plants; 3) loss and recovery due to wildfire; 4) amount of change by human activities. As a result of field surveys and analysis of activity data available domestically, it was found that there was a significant difference in the amount of carbon in biomass according to use type classification and climate zone-soil type classification. Therefore, in order to create an inventory of grassland biomass at the Tier 2 level, a policy and institutional system for making activity data should develop country-specific coefficients for climate zones and soil types.

비엔나영사협약은 해외에서 체포 ․ 억류된 외국인이 자국의 영사로부터 조력 과 지원을 받아 공정한 법절차를 통해서 권리를 보장받고 불이익을 받지 않도 록 재외국민의 영사보호제도를 규정하고 있다. 국제노동기구의 해사노동협약도 선원의 안전과 권리를 보호하기 위한 목적으로 영사보호제도를 규정하고 있다. 이 논문은 두 협약의 영사보호 규정을 검토하고 해사노동협약상 영사보호제도 의 국제법적 한계를 분석하고 국내법적 개선 방안을 제시하였다. 해사노동협약의 영사보호제도는 선박의 기국관할권을 반영하여 기국의 의무를 규정하고 있다. 다국적 선원으로 구성된 선내조직을 선원의 국적국 영사보 호만으로 보호하는데 한계가 있다. 따라서 기국의 관할권을 반영한 해사노동협 약의 영사보호제도는 선원의 권리보장에 있어서 국제법적 의의가 크다. 그러나 해사노동협약의 Code B는 ILO의 감독제도의 직접적인 대상이 되지 않기 때문 에 당사국의 자발적인 국내법적 이행에 의지해야 한다. 대한민국은 비엔나영사협약과 해사노동협약의 당사국으로서 조화로운 국내 법 이행이 필요하나 현행 국내 법령에는 해사노동협약의 영사보호제도가 반영 되어 있지 않다. 따라서 국내법적으로 형사수사규칙을 개정하여 해사노동협약 상의 영사보호제도를 반영할 필요성이 있다. 또한 선원법에서 규정하고 있는 선원인권교육과정의 개편을 통해 선원과 선박소유자의 영사 접촉 ․ 통신에 대한 인식을 개선한다면 한층 선원의 영사보호제도가 강화될 수 있다고 판단된다.

The Asian longhorned tick, Haemaphysalis longicornis, is an ectoparasite of domestic and wild animals, which transmit various pathogens including Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV). They are the predominant hard tick species in Republic of Korea (ROK) and widely distributed throughout ROK. It is known that H. longicornis produce their offspring via two reproductive strategies, bisexual and parthenogenesis. It might affect their population maintenance and vectorial capacity. Parthenogenesis H. longicornis had the insertion of two thymine ‘T’ in mitochondrial 16s rDNA. In this study, we analyzed the proportion of bisexual and parthenogenesis H. longicornis in ROK including thirteen cities: Goseong[GS], Sokcho[SC], Chuncheon[CC], Ganghwa[GH], Samcheok[SCH], Sangju[SJ], Boryeong[BR], Ulsan[US], Gochang[GC], Jinju[JNJ], Jindo[JD], Jeju[JJ], and Seogwipo[SG]. Parthenogenesis individuals predominated from the northeastern are of ROK including Goseong, Sokcho, Chuncheon, Ganghwa, Samcheok, Sangju, Ulsan, and Jinju. Whereas bisexual individuals predominated from the southwestern area in ROK including Boryeong, Gochang, Jindo, Jeju, and Seogwipo. The analysis of haplotype diversity using concatenated sequences of mitochondrial Cytochrome c oxidase subunit I (COI) and Cytochrome B (CytB) revealed that H. longicornis were grouped into two major haplotypes. Two major haplotypes were correlated with bisexual and parthenogenesis, respectively. Likewise, H. longicornis individuals were divided into two clades and each clade were indicated by bisexual and parthenogenesis. The current study provides us an understanding of the genetic characteristics of two reproductive strategies for H. longicornis, which will be led to expand knowledge of the life cycle and population maintaining for H. longicornis.

Rice serves as the staple food for many Asian countries. However, it faces a significant threat from various Hemiptera species, including Nilaparvata lugens, Sogatella furcifera, Laodelphax striatellus, and Nephotettix cincticeps, which can cause devastating diseases. These species are economically significant pests of rice. Traditional morphology-based methods have proven inefficient in accurately distinguishing these pests at the species level. In this study, we present a successful approach for designing species-specific primers and their application in both general and multiplex PCR as well as loop-mediated isothermal amplification (LAMP) assays, widely adopted molecular tools for species identification. Each primer set incorporates a species-specific sequence of at least 2 base pairs in both the forward and reverse primers. These primers have demonstrated exceptional diagnostic accuracy in conventional and multiplex PCR. Additionally, our study showcases the high sensitivity of LAMP, successfully achieving positive amplification with genomic DNA quantities ranging from 100pg to 10pg. In summary, these techniques provide an efficient means of diagnosing planthoppers in a large number of field-collected or individual samples.

담배가루이(Bemisia tabaci)는 국내 주요 농업 해충으로서 고추, 오이, 토마토 등의 시설재배 작물에 큰 피해를 입힌다. 담배가루이는 작물을 흡즙하여 식물체의 상태를 악화시키고 TYLCV(tomato yellow leaf curl virus)등 100 여 종의 바이러스를 매개하며, 배설물을 통해 그을음병을 유발한다. 가장 일반적인 담배가루이의 방제방법은 빠르고 높은 효과를 지닌 살충제 살포이다. 하지만, 지속적인 화학적 방제는 해충의 살충제 저항성을 야기한다. 살충제 저항성은 방제 효율 감소와 농가의 경제적 손실을 일으킨다. 본 연구는 국내 지역별 시설 고추 재배지에서 발생한 담배가루이의 약제별 저항성을 조사하여, 지역별로 효과적인 약제 선정 및 대체 약제 추천 등 지속 가능한 해충관리법을 제시하고자 한다. 담배가루이는 전국 13개(파주, 양평, 화성, 횡성, 당진, 천안, 공주, 예천, 구미, 사천, 남원, 나주, 고흥) 지역에서 채집되었다. 작용기작 별 사용량이 많은 8종을 선정하였으며, 엽침지법을 사용 하여 살충률을 확인하였다. 곤충생장조절제(insect growth regulators, IGR)인 피리프록시펜계 약제는 알을 대상 으로, 그 외 7개 약제는 2령약충을 대상으로 살충률을 확인하였다. 높은 살충력을 보인 약제는 아버멕틴, 밀베마 이신계, 스피노신계, METI살충제, 디아마이드계 약제이다. 아버멕틴, 밀베마이신계는 권장사용농도로 처리 시 최소 84.5%, 최대 100% 살충률을 보였다. 스피노신계는 최소 86.3%, 최대 90.6% 살충률을 보였으나 천안, 파주, 사천에서 각각 59.7%, 66.6%, 79%로 다른 지역에 비해 상대적으로 낮은 살충률을 보였다. METI살충제는 최소 81.1%, 최대 98% 살충률을 보였으나 나주, 예천, 천안, 사천이 각각 61.6%, 68.8%, 68.9%, 69.2%의 상대적으로 낮은 살충률을 보였다. 디아마이드계는 최소 83.9%, 최대 91.9% 살충률을 보였으나 천안과 구미가 각각 56.6%, 59.8%로 상대적으로 낮은 살충률을 보였다. 낮은 살충력을 보인 약제는 네오니코티노이드계, 설폭시민계, 테트 론산 및 테트람산 유도체, 피리프록시펜계 약제이다. 네오니코티노이드계는 최소 14.1%, 최대 41.9% 살충률을 보였다. 설폭시민계는 최소 17%, 최대 42.8% 살충률을 보였다. 테트론산 및 테트람산 유도체는 최소 30.5%, 최대 54.9% 살충률을 보였으나 천안이 14.4%로 특히 낮은 살충률을 보였다. 피리프록시펜계는 최소 55.3%, 최대 64.3% 살충률을 보였으나 횡성이 72.2%로 상대적으로 높은 살충률을 보였으며, 파주가 35%로 상대적으로 낮은 살충률을 보였다. 실험 결과를 통해 살충제 별 살충력의 차이와 지역별로 살충률의 차이를 확인하였다. 본 연구결 과를 통해 효과적인 약제 추천과 지역에 따라 다른 방제 전략 제시에 도움이 되고자 한다.

The DPRK had been upgrading its nuclear weapons capabilities from the past to the sixth nuclear test in 2017, and Kim Jong-un has been in power since the death of Kim Jong-il in 2011, striving to upgrade and diversify four nuclear tests and firing means. In 2022, in particular, DPRK launched more than 40 ballistic and cruise missiles and provoked them in various ways, such as developing solid rocket engines, flying fighter jets, and invading drones. In addition, reprocessing facility activities have been observed again since 2021 at the Yongbyon Nuclear Research Complex. Operational activities such as continuous activities of the 5MWe Yongbyon reactor and the additional construction of new buildings are observed. DPRK’s recent activities could result in nuclear weapons in all except conventional weapons provocations. DPRK has researched and developed nuclear weapons since the 1950s. It has been preparing to operate nuclear weapons, operating nuclear power, and modernizing nuclear power simultaneously. Given the number of nuclear weapons using DPRK’s nuclear materials and various means of missiles, an offensive transition is expected to enable restrictive deterrence strategies that can be used first use and on assured retaliation. In addition, based on the nuclear strategy, which is interpreted as Vipin Narang’s nuclear doctrine and nuclear posture, DPRK is also capable of assured retaliation and asymmetric escalation posture. In particular, considering the continuous activities of the Yongbyon Nuclear Research Complex, which has recently diversified the investment vehicle, and the delegation of nuclear weapon use, it is expected to move differently from the previous one based on the changed nuclear strategy. However, there are clear limitations to interpreting it as a completely assured retaliation and asymmetric expansion nuclear strategy. First, there is a lack of development of atmospheric reentry vehicles that can avoid ICBM interception for assured retaliation capabilities against the United States. Second, there are limitations in the open capacity of nuclear weapons due to the absence of SSBN capabilities. However, delegation to operations at strategy force suggests the possibility of asymmetric expansion strategies. The previous analysis of DPRK’s nuclear strategy and limitations is valid in that the U.S. nuclear umbrella guarantees the Republic of Korea in a strong alliance between the Republic of Korea and the U.S. If the Republic of Korea lacks a nuclear umbrella due to the weakening of the alliance or limits U.S. intervention by having more than dozens of ICBMs, it is considered that DPRK can use a definite confirmation retaliation and asymmetric escalation nuclear strategy. As a response to this, it is the first way to verify and strengthen the validity of the three-axis system (Kill Chain, KAMD, KMPR), second to strengthen the Korea-U.S. alliance, and finally to appeal to the international community and increase consensus. In particular, it is possible to form a consensus of sanctions and condemnation DPRK by expressing concerns about nuclear dominoes caused by nuclear proliferation and arms competition to the international community.

The Republic of Korea has implemented an obligatory vaccination on major livestock breeds to prevent and eradicate nationwide foot and mouth disease (FMD) since the end of 2010. The government has executed massive serological survey to check the immune level of various herds after vaccination, and seropositive rates against FMD virus (FMDV) structural proteins can be measured to assess FMD immunity level. The purpose of this study is to investigate the FMDV serological level of every cattle breeding farm in the country and to determine whether there is a significant difference between groups classified by time, age, and management authority. A total of 5,781 serum samples was collected in 18 cattle breeding farms from 2020 to 2021, and the seropositive rates were measured using PrioCHECK FMDV Type O ELISA kit. Firstly, the cattle breeding farms were classified by which they are managed: the central government, the local government, and the private agency. Every management authority had a seropositive rate of 99.5% or higher. Secondly, the samples were divided into 6 to 12 months old, 12 to 24 months old, and 24 months or more. The 6 to 12 months old group in 2020 showed a significantly low seropositive rate of 98.1%, but it was improved by implementing the enhanced vaccination policy from 2021 to 100%. In summary, there are considerably high seropositive rates including all groups with time, age, and by which they are managed, which means the FMD vaccination in cattle breeding farms is well-managed.

Influenza A viruses (IAVs) are members of the family Orthomyxoviridae and genus Orthomyxovirus. Avian and mammalian species are the host of IAVs, which includes humans and dogs. Canine influenza virus (CIV) is an emerging pathogen that causes severe and acute respiratory diseases in dogs. This study monitored the antigen and antibody against CIV in dogs in the Republic of Korea (ROK) from 2016 to 2021. One thousand and seventy-two nasal swabs and 1,545 blood samples were collected from animal hospitals and animal shelters. Five nasal swabs in 2017 and seven in 2018 from stray dogs were positive for CIV according to RT-PCR. The prevalence of H3N2 CIV ranged from 9.5% to 24.8%, according to the hemagglutination inhibition (HI) assay. On the other hand, none of the serum samples from 2018 to 2021 showed seropositivity against the avian H5, H7, and H9 viruses. The HI titers for H3N2 ranged from 16 to 512. The distribution of HI titer 16–32 was 57.6% in seropositive samples. The pet dogs were vaccinated against CIV, but the stray and military dogs were unvaccinated. In 2017 and 2018, the seroprevalence of CIV in stray dogs was higher than in the other years, and viral RNA was detected in nasal swabs. It may mean previous exposure of stray dogs to CIV. With the increasing number of pet dogs and the close contact between humans and dogs, canines could serve as an intermediate host for transmitting IAVs to humans. Therefore, continuous surveillance of CIV is needed for public health and the potential emergence of novel zoonotic viruses.

Ticks and tick-borne pathogens are among the main sources of interest in veterinary medicine and public health. This review aimed to cover published data on tick species and their geographical distribution in Korea. Over 143 articles regarding ticks were published between 1966 and 2022, and reporting at least 29 species belonging to the family Ixodidae and five belonging to the family Argasidae. Among these, Haemaphysalis longicornis, H. flava, and Ixodes nipponensis were the main genera distributed nationwide, whereas Amblyomma was mostly identified in the southern region. Conversely, only a limited number of Argasidae ticks were present, including the genera Argas and Ornithodoros. Considering the changes in tick distribution patterns due to global warming, continuous nationwide monitoring of ticks is required.

The Ministry of Agriculture, Food and Rural Affairs introduced the Agrifood Voucher in 2020. The Agrifood Voucher is the program that provides vouchers to purchase selected food items with dietary management education. This study aimed to explore value and meaning of dietary management based on the Agrifood Voucher. First, the Supplemental Nutrition Assistance Program of the United States and the Agrifood Voucher of Korea were reviewed. Second, various terms used for describing the purpose of food assistance programs were comparatively reviewed and ‘food and nutrition security’, together with the corresponding Korean term, was proposed to be the most appropriate term for the purpose. Subsequently, the value and meaning of dietary management based on the Agrifood Voucher were presented as enhancing food and nutrition security of the vulnerable. Diverse education programs should be developed and implemented to improve the dietary management capacity of the Agrifood Voucher recipients in order to properly realize the meaning and value of dietary management based on the Agrifood Voucher in the future.