In order to perform the protein analysis using the paraffin sections previous fixed with formalin, we applied the ImmunoMemBlot (IMB) method1) to detect the epitopes of target proteins with specific antibodies. In this study the protein extracts were obtained from the paraffin sections of each representative case of ameloblastoma, adenomatoid odontogenic tumor (AOT), and normal gingiva, and more a protein extract from fresh tissue of ameloblastoma was also compared to evaluate the IMB results used with 24 different antibodies. First of all, in the comparison between the paraffin section extract and fresh tissue extract of ameloblastoma, the latter consistently showed more positive IMB reaction than the former. Meanwhile, the paraffin section extract of ameloblastoma was more comparable with that of normal gingival, disclosing that most of proliferating genes, oncogenes, and apoptosis related genes, i.e., PCNA, CDK4, c-erbB2, CEA, p53, Bax, Bad, FLIP, FAS, Bcl-2, p21, N-ras, MMP-2, MMP-9, caspase-3, -8, -9, were highly expressed in ameloblastoma, but EGFR, HGF, and VEGF were similarly expressed both in the ameloblastoma and in normal human gingiva. On the other hand, the comparison between ameloblastoma and AOT both in the immunohistochemistry and IMB using their paraffin section extracts clearly demonstrated that the ameloblastoma showed more expression of proliferating genes and oncogenes while the AOT showed more expression of apoptosis related genes, i.e., Bax, Bad, FLIP, and caspase-9. Taken together, these data suggest that the IMB can be used for the primary screening of quantitative protein analysis using the paraffin section extract, and that the IMB results could be evaluated in conjunction with the immunohistochemical observation.

  The purpose of this study is to identify the factors affecting intention to use blog. For this end, the individual characteristics (Subject Norm, Perceived Enjoyment, and Self-Efficacy) and the variables used in TAM (Perceived Ease of Use, and Perceived

본 연구는 현재 수행중인 "한국형 포장 설계법 개발과 포장성능 개선방안 연구"의 일환으로 역학-경험적 포장 설계법에 사용될 교통하중 입력변수를 정량화하기 위해서 실시하였다. 교통하중 정량화를 위하여 기존의 ESAL 개념이 아닌 축하중 스펙트럼을 이용하였다. 이를 위하여 교통량 통계 연보내 교통량 자료를 가지고 일반 국도에 대한 대표 축하중 조사지점을 차로별, 지역특성별 구분에 따라 33개 지점을 통계적 방법을 이용하여 선정하였다. 그리고 선정된 대표 지점을 대상으로 축하중 계측기를 이용하여 각 차종에 대한 축하중을 조사하였다. 측정된 축하중 자료는 각각 분류된 조건에 따라서 축하중 스펙트럼으로 표준화하였으며, 그 방법으로 누적밀도함수인 S-커브 곡선을 이용하여 각 조건별 계수를 제시하였다.

본 연구는 돼지 수정란의 체외 성숙 및 체외 배양액의 retinol 첨가 효과를 규명하기 위하여 체외 성숙 및 체외 배양액에 retinol을 첨가하여 수정란의 체외 발달에 미치는 영향을 구명하고자 수행되었다. 체외 성숙 배양액에 retinol을 첨가한 결과 성숙율은 으로 각 처리구 간의 유의적인 차이가 없었다(p>0.05). 체외 수정 후 배반포로의 발달율은 첨가구에서 의 발달율을 나타내어 타 처리구에 비하여 유의적으로(p<0.05) 높게 나타났으며,

The purpose of this study is to identify the factors affecting intention to use blog. Especially, The factors such as subject norm, perceived enjoyment, self-efficacy are individual characteristics to identify in this study. Also, Technology Acceptance Model(TAM) that has been widely used to predict the end-user's acceptance of a new technology is adopted for the study. A model with individual characteristics (subject norm, perceived enjoyment, and self-efficacy) and Technology Acceptance Model variables (perceived ease of use, and perceived usefulness) were used to explain intention to use blog. The suggested model has been empirically tested with questionaries. The result shows that individual characteristics such as perceived enjoyment and self-efficacy and factors of technology acceptance model have significant impact on users' intention using blog.

  This paper is concerned with a six sigma application to non-manufacturing process. Based on the comparison to manufacturing process, non-manufacturing process has some specific characteristics. The main focus of this study is to introduce an empirical c

According to Korea was re-elected as ICAO council member state at the 35th session of the assembly, there are more contributions and roles of international aviation society. Being developed technology and grown international transportation, world is focusing to aviation safety, Safety Management System(SMS) is systematic approach to manage risks caused by human and organizational factors. ICAO is specified that 'As of 24 November 2005, certified aerodrome shell have in operation a safety management system.' at Annex 14 to implement it airports as a first step. This study introduced SMS principle, analysed SMS's of advanced states and developed appropriate Korean Aerodrome SMS Model in our cultural and industrial circumstances.