Background: To obtain useful safflower resources for breeding and research of functional materials, the present study was conducted to determine fatty acid compositions and antioxidant activities of 281 safflower accessions collected from East Asia including South Korea. Methods and Results: Lipid contents and fatty acids compositions were evaluated using soxhlet extraction and gas chromatography, respectively. A antioxidant activities were analyzed using a spectrophotometer. The evaluation range of safflower accessions showed very wide variation. In terms of lipid contents, the China accessions were higher than the collection from other regions, whereas antioxidant activities did not differ among regions. The result of the principal component analysis showed that the first and second principal component cumulatively explained 90.6% of the total variation. In clustering the safflower accessions, the tree showed four major clades. Group II (16 accessions) was high in lipid content, oleic acid and linoleic acid, whereas group III (50 accessions) exhibited higher antioxidant activities than other groups. Conclusions: It was recommended that the China collections be utilized as a useful resource for research on functional oil materials. These results provided valuable information for safflower breeders and researchers of functional food.

Identification of Fusarium oxysporum f. sp. raphani and investigation on fusarium wilt development by isolates and inoculation methods were conducted to establish a screening method for fusarium wilt-resistance in radish (Raphanus sativus L.) germplasm. Pathogenicity of F. oxysporum f. sp. raphani isolate, Heonggye-1 and Heonggye-2, to radish plants was confirmed by seedling test. Radish seedlings were inoculated by root-dipping and soil-drenching with or without root-wounding. For Heonggye-1 isolate, mean disease indexes were 4.13 and 3.91 by root-dipping and soil-drenching with root-wounding, respectively, but those were 1.87 and 1.88 without root-wounding. For Heonggye-2 isolate, mean disease indexes were 3.83 to 4.37 regardless of inoculation methods. Two-hundred sixty accessions of radish germplasm collected from 9 countries of Asia and Europe were evaluated for fusarium wilt-resistance by soil-drenching with root-wounding with Heonggye-2 isolate. Fifty-four resistant accessions with higher than 70% of the percentage of resistant seedlings in accession (PR) and lower than 20% of the percentage of susceptible seedlings in accession (PS) was found. Eighteen susceptible accessions with lower than 20% of PR and higher than 50% of PS were selected. These accessions could be used as breeding and research materials after re-evaluation of disease-resistance and characterization of agronomical traits.

본 연구는 베트남 결혼이민여성들의 자녀 외가방문에 대한 요구를 조사하여 향후 결혼이민가정 자녀들을 위한 교육 프로그램 개발 및 실행에 필요한 기초자료를 구축하는데 목적이 있다. 이를 위해 자녀 외가방문의 목적과 베트남에서 경험해야 할 활동에 대한 결혼이민여성들의 인식을 살펴봤다. 연구대상은 한국여성재단 2013 ~ 2014년도 다문화아동 외가방문 지원사업에 지원한 베트남 결혼이민여성 151명의 지원신청서이다. 연구결과, 베트남 결혼이민여성들의 자녀 외가방문의 목적은 첫째, 베트남 문화와 언어에 관심 갖고 학습하기를 바람, 둘째, 외가가족을 만나 서로 사랑하기를 바람, 셋째, 다문화가정의 자녀로서 자긍심 갖기를 바람, 넷째, 엄마를 좋아하고 이해하기를 바람, 다섯째, 한국에서의 일상생활에 감사한 마음을 갖기를 바람, 여섯째, 한국과 베트남의 발전에 기여하는 인재로 성장하기를 바람으로 나타났다. 베트남 결혼이민여성들이 자녀들에게 필요하다고 생각하는 경험은 첫째, 베트남 문화체험, 둘째, 외가가족과의 유대감 형성, 셋째, 엄마의 어린 시절 경험 공유로 나타났다. 본 연구결과는 다문화가정의 엄마와 자녀들이 외가가족들과 지속적으로 교류하고 엄마나라에 대한 관심을 갖게 하는 프로그램의 개발과 보급에 국가적 지원이 시급함을 시사한다.

The objective of this study was to select yogurt starter from Korean traditional fermented foods. The 2 strains (KM24, KM32) among 50 strains of isolated lactic acid bacteria selected as starter based on milk clotting ability, antimicrobial activity against various pathogens, tolerance in artificial gastric and bile juice and growth in 10 % skimmed milk. The strains were identified as Lacobacillus plantarum (KM32) and Pediococcus pentosacesus (KM24) by 16S rRNA gene sequencing. Viable cell number of yogurt fermented with mixed strains (KM24 and KM32) was 9.66 log CFU/mL after fermentation for 48 h and maintained 109 CFU/mL during fermentation for 72 h at 37℃. The pH and titratable acidity of mixed cultured yogurt were 4.25% and 0.83% after fermentation for 48 h at 37℃, respectively. The physico-chemical characteristics of mixed cultured yogurt after fermentation for 48 h were 38.45 μg/mL (polyphenol content), 48.57% (DPPH radical scavenging activity) and 465.40 cp (viscosity), respectively. The mixed cultured yogurt maintained 109 CFU/mL of lactic acid bacteria during storage 10 days at 4℃. The viable cell number of yogurt prepared with mixed culture(KM32+KM24) maintained higher and than that of control (L. casei) during storage. These results indicated the potential use of selected strains (KM32+KM24) isolated from kimchi as a yogurt starter with strong acid tolerance and probiotics properties.

The purpose of this study was to investigate the physiochemical properties of Doenjang was fermented by added with fungi and protease. The moisture content and pH of Doenjang added with protease (WP) were lower than those of control w/o protease while the contents of titratable acidity, reducing sugar, and amino-type nitrogen in WP were higher than control. The α-amylase activities of Doenjang added with single and mixed Protease B were the highest at 4 weeks of fermentation period and protease activity of WP was about 4 times higher than that of control. The 4-9 kinds of free amino acids (proline, isoleucine, leucine, and phenylalanine etc.) in WP was increased in comparison with control. The DPPH radical scavenging activity and total polyphenol content were higher in WP than control. Total aerobic bacterial and fungal numbers were decreased depending on fermentation time regardless of addition of protease. In conclusion, the protease can be used as additives improving the quality and taste of fermented Doenjang.

The purpose of this study was to examine the effects of long-term endurance exercise and Salvia miltiorrhiza vinegar on body composition and insulin resistance of high-fat diet (30% carbohydrate, 50% fat and 20% protein) induced obese rats. After 8 weeks of high fat diet (50% of total calories), rats were divided into 4 groups (sedentary group, n=10; exercise group, n=10; Salvia miltiorrhiza vinegar group, n=10; exercise+Salvia miltiorrhiza vinegar group, n=10) for 8 weeks. Body weight, body composition, diet intake volume, oral glucose tolerance test, plasma total cholesterol were measured. The results showed that Salvia miltiorrhiza vinegar plus endurance exercise training for 8 weeks significantly improved body weight control, visceral fat weight, and insulin resistance. However, only Salvia miltiorrhiza vinegar treatment did not significantly improve body composition and insulin resistance. In addition, there was no additive by the combination of Salvia miltiorrhiza vinegar and endurance exercise in insulin, body fat, and total cholesterol. The reduction of body fat, glucose, insulin and cholesterol by combination was resulted from the exercise. These results suggest that Salvia miltiorrhiza vinegar has slight effect on anti-hyperglycemia and anti-obesity.

게임에 대한 사회적 인식의 변화와 소프트웨어 교육, 코딩교육에 대한 중요성이 높아지는 현 시점에서 교육용 게임을 이용한 코딩교육에 대한 연구가 활발히 진행되고 있다. 하지만 게임의 완성도, 게임성이 실제 학습자에게 미치는 영향과 학습 성취도에 대한 연구는 미비한 실정이다. 본 연구는 스토리강화, 캐릭터의 다양성, 향상된 그래픽 요소, 풍부한 보상 장치, 업적 시스템 추가 등의 게임성의 완성도 차이가 학습자의 교육태도, 학습몰입, 교육의 만족도, 선호도 등에 미치는 영향에 대해 분석하였다. 이는 교육용 게임의 질적 향상이 학습에 미치는 영향에 대해 고찰하고, 게임 설계 시 게임성과 완성도를 중요하게 고려해야 함을 제안한다는 데 의의가 있다.

기능성 제빵 소재로써 연잎의 이용 가능성을 검토하기 위해 연잎 분말을 1, 2 및 3%를 첨가하여 식빵을 제조하면서 제빵 특성 및 빵의 품질특성을 조사하였다. 연잎 분말의 조회분, 조지방 및 조단백질 함량은 각각 10.68, 3.40 및 22.31%로 나타났으며, 연잎 분말 95% 에탄올 추출물 1%(w/v) 농도에서 총 폴리페놀 함량은 270.85 μg/mL였으며, 0.1%(w/v) 농도에서 DPPH 라디칼 소거능은 90.1%를 나타내었다. 연잎 분말을 첨가한 식빵 반죽과 식빵의 pH는 연잎 분말 첨가량이 증가할수록 감소하였으며, 산도는 증가하였다. 반죽의 부패 팽창도는 LLP-1과 대조구는 유의적인 차이는 나타나지 않았다(p<0.05). 연잎 분말 첨가 식빵의 굽기 손실률은 대조구에 비해 낮았으며, 식빵의 무게는 연잎 분말 첨가량이 증가할수록 증가하였고, 부피는 감소하였다. 식빵의 경도는 대조구가 가장 낮았으며 연잎 분말 첨가량이 증가할수록 경도도 높았다. 연잎 분말 첨가 식빵의 DPPH 라디칼 소거능과 총 폴리페놀 함량은 각각 17.08-40.13%와 615.01-885.13 μg/mL의 범위를 나타내어 대조구에 (9.26% 및 472.20 μg/mL)에 비해 높았으며, 연잎 분말 첨가량이 증가할수록 식빵의 DPPH 라디칼 소거능 및 총 폴리페놀 함량도 유의적으로 증가하였다(p<0.05). 연잎 분말을 첨가한 식빵의 종합적 기호도는 대조구가 3.64로 가장 높았으며, 다음은 LLP-1(3.56)으로 나타났으나, 두 시료간의 유의적인 차이는 나타나지 않았다(p<0.05). 식빵의 기능적 특성과 관능적 특성을 고려하였을 때, 연잎 분말은 1% 첨가하였을 때가 가장 적당하다고 판단되며, 최대 2%를 넘지 않은 것이 바람직할 것으로 사료된다.

The purpose of this study was to investigate changes in the amino acid content and physicochemical properties of Cheonggukjang prepared by using various soybean cultivars (Daewon, Deapung, Seadanbeak, and Taekwang) and a functional microorganism (Bacillus subtilis HJ18-9). These soybeans were conventional Cheonggukjang (control) and Cheonggukjang fermented with Bacillus subtilis HJ18-9 (treated). The moisture contents of steamed, control, and treated soybean were 62.45~67.12%, 63.28~67.14%, and 64.50~66.87%; amino-type nitrogen contents were 6.53~24.25 mg%, 27.63~122.09 mg%, and 37.29~133.48 mg%; and ammonia-type nitrogen contents were 26.92~47.95 mg%, 45.45~156.36 mg%, and 28.02~121.13 mg%, respectively. The umami taste associated with several amino acids (aspartic acid　and glutamic acid) in Cheonggukjang was lower than that for steamed soybeans, while the bitter taste from amino acids (methionine, valine, isoleucine, leucine, and phenylalanine) was higher than that for steamed soybeans. The result of sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the molecular weight of steamed soybeans was less than 100 kDa, while control and treated groups showed low molecular weights below 34 kDa, confirming their protein hydrolysis to small molecular weight. These results are information for developing functional fermented soybean paste and diversification using soybean cultivars.

This study presented the change of quality characteristics of Doenjang with various salt concentrations (4, 8, 15, and 20%) during 12 months. The pHs of Doenjang during fermentation were gradually increased for 12 months, whereas the pHs of Doenjang with 4 and 8% salt increased rapidly. Titratable acidity of Doenjang with 4 and 8% salt was high at initial time, while Doenjang with 15 and 20% salt was high at the 9 and 12 months. The amino-type nitrogen content of Doenjang with 4 and 8% salt was increased and α-amylase activity of all Doenjang was decreased during whole fermentation period. The protease activity of Doenjang with 4 and 8% salt was higher than Doenjang with 15 and 20% salt until 7 months. Total aerobic bacteria number 8.0~9.1 log CFU/g for 12 months, which was steady during fermentation period. Doenjang with 8% salt showed the highest score in umami taste whereas the lowest score in bitterness, astringency, and sourness tastes between 5 and 12 months. In conclusion, Doenjang with 8% salt was a suitable concentration for low-salt fermented foods.

본 연구는 한국과 싱가포르, 인도, 미국의 자유무역협정(FTA) 발효로 인한 양자간 교역증진효과를 통계적으로 비교 분석하고자 한다. 다수의 선행연구에서 양자간 교역 증대효과 추정시 활용된 중력모형(Gravity)을 기본 방법론으로 패널분석(Panel analysis)을 통해 한 국과 FTA 발효국(싱가포르, 인도, 미국)을 효과집단으로, 미발효국은 통제집단으로 구분하여 총 20년의 기간을 설정하여 FTA 발효의 순수효 과를 추정하였다. 자유무역협정의 교역증대효과 분석을 위해 우리나라와 FTA가 발효된 3개 국가(효과집단)와 2015년 기준 우리나라 벌크물 동량 교역 상위 90%의 비중을 차지하는 27개 국가(통제집단)의 횡단면 및 시계열로 구성된 패널자료를 사용하였다. 분석 결과 FTA 발효는 우리나라 벌크물동량 증대에 기여한 것으로 나타났다. 특히 본 연구의 대상국가인 싱가포르, 인도, 미국과는 자유무역협정 발효로 인한 무역 창출효과가 발생하였고 ASEAN 및 NAFTA+3의 경우 무역전환효과가 발생하였다. 한편 자유무역협정 이외의 GDP, 1인당 GDP 변수는 벌크 물동량과 정(正)의 효과를 보이는 것으로 나타났으며, 거리 변수는 물동량과 부(負)의 효과를 보이는 것으로 분석되었다. 마지막으로 국가별 패널자료 분석 결과 하우스만 검정 및 LR검정을 시행하였으며, 고정효과모형이 임의효과모형보다 적합한 것으로 나타났다.

Quality characteristics of Yanggaeng prepared with water extract (CRE) and powder (CRP) of roasted coffee ground (CR) were investigated. Total polyphenol content and DPPH raidcal scavenging ability of water extract of CRE were 13.52 g/mL and 78.75%, respectively. The pH ranges of Yanggaeng prepared with CRE (CREY) and CRP (CRPY) were 7.10~7.29 and 6.95~7.15, respectively. The DPPH radical scavenging activities of CREY containing 0.1~1.0% CRE showed 8.77~43.10% and CRPY containing 0.1~1.0% CRP showed 5.28~14.92%. The total polyphenol contents and DPPH radical scavenging activity of CREY and CRPY increased significantly with increasing CRE and CRP concentrations (p<0.05). Sensory evaluation which includes taste, flavor, texture, and overall acceptability of CREY and CRPY were higher than that of control. The overall acceptability showed the highest levels in Yanggaeng containing 0.5% CRE and containing 0.3% CRP. These results indicate the potential use of roasted coffee ground residue as a valuable resource for development of side menu in coffee restaurants.

This study was conducted to compare the community structure and biodiversity of ground-dwelling invertebrates between conventional and organic paddy fields’s levees. Ground-dwelling invertebrates were collected using a pitfall trap every month for two years (2010-2011) in levees of conventional and organic paddy fields. The numbers of species and individuals were higher in organic paddy field than in conventional paddy field. For the pests, the treatment of insecticide and herbicide reduced the number of Delphacidae, and Chrysomelidae, but did not affect the other pests such as Chronomidae, Culicidae, Thripidae, and Aphididae. For the natural enemies, the treatment reduced the number of individuals of most of enemy's taxon (except only one taxonomic group, Ichneumonidae) in the levee of conventional paddy field.

The characteristics and quality changes of sauces containing different concentrations (5, 10, and 15%) of fermented Oenothra biennis juice (FOBJ) were studied for its potential as a new meat seasoning suace. pH of the seasoning sauce was ranged from 5.23 to 5.37. Sugar content of seasoning sauces increased decreasing on increase of FOBJ concentration while the color (L, a, b) decreased. DPPH radical scavenging ability and TBARS values of the seasoning sauce was increased significantly by the addition of FOBJ (p<0.05). The total bacteria number of the seasoning sauces was approximately 3 log CFU/g while coliform bacteria were not detected in all tested sauces. The sauce containing 5% FOBJ showed the highest value in taste, color, overall acceptability among all tested sauces. The pH and titratable acidity of the meat seasoning sauces was not changed significantly during 25 days at 10℃. The total bacteria number of the seasoning sauces was steadly 3.62~3.83 log CFU/g for 25 days at 10℃. The number of coliform was not detected during storage periods. These results suggested the possibility of development of new meat seasoning sauce containing FOBI.

본 연구는 시판 장류의 알코올 함유량 및 관련 품질특성 을 파악하고자 산도, pH, 염도, 환원당, 알코올 함량 및 효모 수를 조사하였다. 산도는 재래식 된장과 간장이 개량식보 다 낮게 나타났고, 재래식 고추장이 개량식보다 높게 나타 났으며, pH는 재래식 된장과 고추장이 개량식보다 낮게 나타났고, 재래식 간장이 개량식보다 높게 나타났다. 염도 는 재래식 장이 개량식 장보다 모두 높게 나타났고, 환원당 과 알코올은 재래식 장이 개량식 장보다 모두 낮게 나타났 다. 또한 주정 무첨가된 시료 중 알코올이 검출된 장류 중에 효모수를 측정한 결과 검출 유무가 알코올 함량과 연관성이 없어 효모의 존재만으로 알코올을 생성하였다고 판단할 수 없었다. 지금까지 결과를 종합해보면 개량식 된장에 비 해 낮은 환원당을 나타낸 재래식 된장의 경우 알코올 발효 의 기질로 사용될 당이 부족하기 때문에 낮은 알코올 값을 보인 것으로 사료되고, 개량식 간장의 경우 재래식 간장보 다 낮은 염도를 나타내기 때문에 균의 활동이 비교적 활발 하여 산도와 알코올 함량이 높은 것으로 사료된다. 할랄식 품 시장 진출을 위한 알코올을 함유하지 않은 장을 만들기 위해서는 난분해성 당을 사용하고 염도를 높여 균 활동을 억제하는 추가적인 연구가 필요할 것으로 보인다.