국립공원 내 양서류 로드킬(Roadkill) 현황을 파악하기 위해 2006년부터 2008년까지 3년 동안 24개 노선을 대상으로 조사를 실시하였다. 그 결과 총 2목 5과 9종 1,748개체가 차량에 의해 로드킬 된 것으로 나타났으며, 년차별 비교결과 2006년에 비해 2007년, 2008년에 로드킬이 감소한 것으로 나타났다. 또한 3월부터 6월 사이에 북방산개구리, 두꺼비, 물두꺼비가 집중적 피해를 입은 것으로 나타났으며 "산림-하천" 및 "산림-산림" 환경에서 교통사고가 집중 발생하였고, 덕유산국립공원 국도 37호선, 속리산국립공원 지방도 517호선, 오대산국립공원 국도 6호선, 월악산국립공원 지방도 597호선 등 총4개 노선에서 집중적으로 로드킬이 발생하였다. 따라서 이 지역의 주요 로드킬 대상종이 북방산개구리와 물두꺼비로 확인됨에 따라서 이들 종에 대한 저감대책 수립이 시급한 것으로 판단되었다.

Patulin, a mycotoxin mainly produced by Penicillium and Aspergillus, is found in various foods. In the present, a maximum acceptable level for patulin is established at 50 μg/kg(ppb) in apple juices and apple concentrates in Korea. But patulin may be detected in foods produced with other fruits. In the present study, patulin contamination was analyzed in 520 samples of fruit juices and beverages, and 50 samples of fruit juice concentrates. High performance liquid chromatography(HPLC) was applied to quantitatively analyze patulin levels in samples and liquid chromatography-mass spectrometry(LC/MS/MS) was used to remove false positive results. The results showed that three samples of 520 fruit juices and beverages and five samples of 50 fruit juice concentrates were contaminated by patulin, 9.8 - 18.0 μg/kg and 4.7 - 18.2 μg/kg respectively. Contaminated samples were produced with apple, orange or pear. This indicates that it is necessary to extend the regulatory range of patulin. In the other hands, the present study confirmed the effectiveness of LC/MS/MS analytical method to remove false positive results.

안토시아닌(Anthocyanin)은 플라보노이드계 화합물의 한 부류로 항산화, 항암 및 항궤양, 항당뇨, 중금속해독, 시력보호, 콜레스테를 저하 등의 다양한 생리활성을 가지는 것으로 보고되어 있다. 죽상경화과정은 염증성 사이토카인의 분비 또는 혈관손상으로 인한 백혈구의 부착과 이동을 통해 시작된다. 본 연구는 이러한 죽상경화의 초기과정에서 안토시아닌 혼합물 중 single compound인 delphinidin chloride (DC) 인간혈관 내피세포

당뇨망막병증은 서구에서 성인들의 실명을 일으키는 원인이다. 당뇨병이 있을 때 고혈당증은 여러 세포 형태에서 세포자연사를 유도하지만 그 기작은 명확하게 밝혀지지 않았다. 본 연구의 목적은 인간망막 내피세포에서 고혈당 포도당이 세포자연사에 미치는 영향에 대하여 알아보았다. 망막 내피세포는 5, 25, 50 mM 포도당이 포함된 IMDM배지에서 37℃, 5% CO2조정된 항온기에서 24, 36, 48시간 동안 배양하였다. 여러 농도의

Naringenin은 flavonoid구조의 감귤류 과피에 다량 함유되어 있으며 항암 및 항산화 등의 다양한 생리활성을 가지는 것으로 보고되었다. 이에 본 연구에서는 HT-1080 섬유육종세포의 전이에 대한 영향을 조사하였다. 먼저 Naringenin이 암세포의 이동에 미치는 영향을 알아보기 위해 migrationassay를 한 결과, Naringenin이 암세포의 이동을 농도 의존적으로 억제시켰다. 암의 전이에 있어서 매우 중요한 역할을 하는 단백질분

As Korea has approached the aging society, older Koreans have become an important force in restaurant sales today. To succeed with this silver market, it is important for restaurant managers to know who they are and what factors influence older Koreans' eating-out decision making process. The purpose of this study was to investigate the effect of the silver consumers' education level on eating-out decision making process. Data were collected from 178 older consumers above 55 years old and analyzed using the descriptive statistic analysis, MANOVA, and one-way ANOVA. The results showed that the elderly consumers' education level significantly influenced the decision making process in determining where to eat out. Significant differences were found in the Problem Recognition Step(Wilks' Lambda=0.817, F=2.991), Information Search Step(Wilks' Lambda=0828, F=2.218), Alternative Evaluation Step II(Wilks' Lambda=0.741, F=3.596), Purchase Decision Step(Wilks' Lambda=0.859, F=2.223), and the Post-Purchase Behavior(Wilks' Lambda=0.885, F=1.780). The higher education level was, the more directly involved in the eating out decision process. The elderly consumers with university education were likely to 'propose to eat out by themselves'(F=9.346), to obtain restaurant information from the 'printed materials'(F=7.452), to go to 'family restaurant'(F=9.057), 'Japanese restaurant'(F=8.7891) and 'fine dining restaurants'(F=3.936), and to directly express their emotion when they had complaints about restaurant service(F=3.206). In conclusion, older Koreans will become more healthy and wealthy which means the dining out activity will be an important part of their life to socialize with people. Therefore, food service operations should consider the elderly consumers' needs and expectation of restaurant services and actively position themselves for this new market segment.

Presbyope has not clear vision with elderly changes. To introduce the essential preliminary examination for presbyope, we performed preliminary tests in 190 elderly patients with case history, slitlamp examination, pinhole test, Hirschberg test, pursuit, stereopsis, pupil reaction, Worth-4 dot test, Amsler grid test, dominant eye test. To perform an examination, the case history is clitical as it guides the whole examination. Chief complaints were epiphora, decreased visual acuity, floaters, hyperemia, eye pain, etc. From the information gained in the case history, we deduced a list of tentative diagnosis. Throughout the examination we decided whether additional tests. The 18.9% of patients didn't have improve visual acuity in pinhole test. Cause of reduced visual acuity in pinhole test was opaque of refractive media such as cataract or pterygium. In 44.7% of patients, stereoauity falls below 40s of arc. The 6.8% of patients have suppression of right or left eye. The 28.9% of patients have metamorphopsia or scotomas in Amsler chart test. Therefore, pinhole visual acuity helps to differentiate ocular pathology from lack of refractive media clarity. Amsler chart is also useful for monitoring age related maculopathy. We suggest that Jones dye test for tear drainage, pinhole test, Amsler grid test and Worth-4 dot test are essential for presbyope.