본 연구진은 메틸말리올라이드가 인간 피부 세포주인 HaCaT 세포에서 NRF2를 유도하고 이를 통하여 항산화 효과를 나타내는지 알아보았다. MTT assay를 통하여 메틸말리올라이드는 10 μ M 농도에서 24 h 동안 HaCaT 세포에 처리하여도 HaCaT 세포 독성을 나타내지 않는 것을 확인하였다. 본 연구진이 구축한 HaCaT-ARE-GFP-luciferase 세포에 메틸말리올라이드를 처리하고 루시퍼라아제 활성을 측정한 결과 메틸말리올라이드는 양성 대조군인 레스베라트롤보다 ARE 결합에 따른 루시퍼라아제 활성을 더욱 강하게 증가시키는 것을 확인하였다. 또한 HaCaT 세포에 메틸말리올라이드 처리 시 NRF2에 의하여 유도되는 항산화 단백질인 HO-1과 NQO1 mRNA 및 단백질 발현이 통계적으로 유의하게 증가하였다. 마지막으로 메틸말리올라이드는 HaCaT 세포에서 TPA에 의해서 유도된 DNA 및 지질의 산화를 강력하게 억제하였다. 결론적으로 본 연구는 메틸말리올라이드가 NRF2 유도를 통하여 피부 산화적 스트레스를 억제하며 이는 메틸말리올라이드가 신규 항산화 화장품의 소재로 적합하다는 것을 시사한다.

인성광상은 옥천누층군의 황강리층 내에 발달한 열극 충진 함 금-은 및 베이스 메탈 석영맥 광상이다. 광상 생성에 기여한 맥들은 녹니석화, 규화작용, 견운모화 그리고 탄산염화 작용 등 다양한 변질양상 및 광석 광물에 따라 네 시기로 구분된다. 첫 번째 시기(stage 1)에는 자형의 황철석 및 유비철석과 함께 투명하고 경제적 광석이 나타나지 않는(barren) 석영맥이 나타난다. 두 번째 시기(stage 2)에는 자형 석영 결정의 석영맥이 형성되며, 유비철석, 섬아연석, 황동석, 황철석 및 방연석이 나타난다. 세 번째 시기(stage 3)에는 유백색 석영맥과 소량의 섬아연석이 나타난다. 네 번째 시기(stage 4)에는 자형의 석영 결정을 포획한 방해석맥이 나타나며, 모암 내 탄산염 변질과 함께 소량의 유비철석, 황철석, 방연석, 섬아연석 및 황동석이 산출된다. 각 시기별 유체 포유물은 주로 염 결정이 포함되지 않은 다양한 밀도의 수용액 유체 포유물이 대부분이다. 각 시기의 균질화 온도(Th: ℃)와 염 농도(NaCl equivalent wt%)는 다음과 같다; 시기 1 (270~342 ℃ 그리고 1.7~6.4 wt%), 시기 2 (108~351℃ 그리고 0.5~7.5 wt%), 시기 3 (174~380 ℃ 그리고 0.8~7.5 wt%) 그리고 시기 4 (103~265 ℃ 그리고 0.7~6.4 wt%). 넓은 범위의 균질화 온도와 염농도는 광화 유체가 천수의 혼입에 의한 냉각과 희석이 있었을 가능성을 지시한다. 0.5~1.5 km의 인성광상의 계산된 고심도는 천열수 환경을 지시한다.

Background : Miscanthus sinensis is a C4 crop with high photosynthetic efficiency and is known as a representative cellulose-based bio-energy crop originating in East Asia such as Korea, Japan, and China. It also has a high resistance to environmental conditions such as low temperature and drying and pests. However, many studies on the antioxidant activity of the M. sinensis have not been conducted. Therefore, this study was conducted to measure total phenol and total flavonoid contents of the M. sinensis accessions collected from some countries and to select the M. sinensis accessions with rich total phenol and total flavonoid contents. The results of this study can be used as basic data for future breeding. Methods and Results : This study carried out the total phenol and total flavonoid contents analysis of M. sinensis collected from different country to preserve genetic resources. Total 205 accessions of M. sinensis were collected from the USA, China, Korea, Japan, Thailand and Zambia. Their leaves were extracted with 100% methanol and concentrated at 45℃. To determine total phenol and total flavonoid contents, we measured the total phenolic content and total flavonoid content. The highest total phenolic conten t(81.04 ± 0.02 ㎎·GAE/g) showed in the accession (PMS-178) from China. The highest total flavonoid content (32.66 ± 0.01 ㎎·QE/g) showed in the accession (KMS104) from Korea. On the other hand the lowest total phenolic content (1.11 ± 0.00 ㎎·GAE/g) and the total flavonoid content (0.81 ± 0.00 ㎎·QE/g) were obtained from the accession (M. sinensis ‘Super Stripe’) from USA. Conclusion : In this study, we analysed the total phenolic and total flavonoid contents by country of origin.

In this study, as a part of the BCP (Business Continuous Plan), the performance tests of the rocking wall and the steel damper were carried out. The rocking wall was made of concrete block. The concrete block was connected to the floor by two hinges. However, in the performance test, the hinge part was destroyed and the experiment was finished. This will be due to the fact that the hinge has not rotated, and will be retested after reinforcement of the concrete block.

In this study, literature review on the method of reinforcing the special shear wall boundary element was carried out. As a reviewing results of two studies, it was evaluated that it is possible to reinforce the boundary element using rectangular steel pipe, H-shaped section steel and lattice material.

Lung cancer, the most common malignant disease worldwide, is the predominant cause of cancer deaths, particularly amongst men. Therefore, various researchers have focused on the growth inhibitory effects of medicinal plants used in traditional Korean medicine. This study aimed to investigate the growth inhibitory effects of ethanol extracts of Rubiae radix, Inulae flos, Nelumbinis receptaculum, Astilbe radix, and Lagerstroemia flos on NCI-H1229 cells. Method and Results: The viability of NCI-H1229 cells was evaluated in vitro using an MTS assay. Treatment with the ethanol extracts of the selected medicinal plants at 500 ㎍/㎖ reduced NCI-H1229 cell viability and increased apoptotic cell death and caspase-3 activation. In addition, treatment with ethanol extracts of Inulae flos and Astilbe radix increases DNA fragmentation, as measured by the TUNEL assay. Conclusions: These results indicated that ethanol extracts of Rubiae radix, Inulae flos, Nelumbinis receptaculum, Astilbe radix, and Lagerstroemia flos exhibited growth inhibitory effects, inducing apoptotic cell death, DNA fragmentation and caspase-3 activation in NCI-H1229 cells. Therefore, these medicinal plant extracts may be used in the development of natural medicines to inhibit the growth of lung cancers. However, further study is needed to determine the active ingredients of the ethanol extracts from medicinal plants that are reposible for the inhibitory effect on lung cancer cell grwoth.

암반사면을 안전하고 효과적으로 해석하기 위해서 암반의 역학적 특성을 면밀하게 조사해야 한다. 하지만 클리노미터를 사용한 절리조사의 한계점으로 인해 이를 보완한 새로운 측정법의 연구가 필요하다. 본 연구에서는 절리방향의 특성을 분석하기 위해 절리의 방향성을 현장에 적용할 수 있는 절리조사 측정장비를 개발하였다. 개발된 측정장비는 해석 소프트웨어와 하드웨어로 구분된다. 하드웨어는 암반 절리 방향성을 측정하는 측정모듈, 측정자료를 전송하는 전송모듈로 구성되었다. 소프트웨어는 측정모듈을 통해 얻은 데이터로부터 절리의 방향성을 분석하기 위해 개발하였으며 Drone Joint Orientation Survey Measurement로 명명하였다. 개발된 측정장비는 접근이 어려운 지역 등 조사자가 측정할 수 없는 경우에 현장적용성이 양호하며 절리의 방향성에 대한 실내시험결과를 효과적으로 분석할 수 있었다.

This study aimed to provide fundamental data for enlargement strategy of Ramsar site through the analysis of designation properties about 22 sites which has been registered for 20 years since Yongneup (Mt. Daeam) was designated as the first Ramsar site in Korea. Designation properties were analyzed on the basis of Ramsar Information Sheet and ecological properties was mainly analyzed in relation to designation criteria. Designation of Ramsar wetland was concentrated before and after Changwon Congress in 2008 and in terms of area 11 sites are below 1 ㎢ and 5 sites are above 10 ㎢. The average number of applied designation criteria was 2.86 and Criterion 2 (threaten species), Criterion 1 (representativeness and rareness of wetland type) and Criterion 3 (biodiversity) are mainly applied. In terms of protection species, internationally recognized species were 18 species on IUCN redlist, 12 species on CITES and 13 species on CMS, and nationally recognized species were 76 species on endangered species designated by Ministry of Envirionment, 83 speceis on national redlist and 71 speceis on endemic species respectively

Background : Bokbonja the Korean black raspberry (Rubus coreanus Miquel) fruit derived product, which is native to Korea. They contain potential anti-aging, anti-oxidants, depurative activities. Bokbonja refers to an immature berry of Rubus coreanus in the Korean pharmacopeia, Rubus chingii in Chinese pharmacopeia. Recently several Rubus species are available in the Korean drug markets which could easily find their way into drug prescriptions. Methods and Results : Hence we tried to detect the presence of these contaminating species in bokbonja products using SNP marker assisted by multi-plex PCR. We found a SNP region in the 26S region of these species and evaluated their potential to discriminate Rubus species. We designed a set of primer pairs such as, BokR primer has distinguished R.coreanus by producing a band at 852 bp and the primer sanF has differentiated R. crataegifolius by amplifying a band at 129 bp, while chiF has produced a band at 83 bp to distinguish R.chingii. These primer pairs effectively distinguished the bokbonja samples collected from various local markets as well as form drug store in Korea as well. The results were shocking as the bokbonja (R.coreanus) has a limited usage in Korea and either R. crataegifolius or R.chingii has been found in these samples. Taken together the primer pairs bokR, sanF, chiF along with 26S universal primers could effectively distinguish Rubus species in a single multiplex PCR reaction. Conclusion : Our results based on the 26S rDNA derived SNP region have shown that, the usage of R. coreanus is extremely limited and its often mistaken or adulterated with R. crataegifolius and R. chingii. It presses a panic situation in Korean peninsula to preserve bokbonja and the species R.coreanus which is endemic and superior in efficacy.

바이오매스는 지속가능한 에너지원의 일종으로 다양한 공정에 따라 바이오 에탄올, 바이오 디젤, 바이오 가스 등으로 회수하여 석유자원의 대체제로 사용 가능하다. 그 중 목질계 바이오매스는 주로 셀룰로오스, 헤미셀룰로오스, 리그닌으로 이루어져 있으며, 셀룰로오스는 바이오 에탄올 등의 연료화를 통해 이용되고 있으나, 리그닌의 경우 펄프 공정 내 부산물로 여겨져 단순 연소를 통한 열에너지원으로서의 역할을 하고 있다. 하지만 리그닌은 자연에서 가장 많이 존재하는 페놀성 고분자 화합물의 일종으로, 이를 이용한 페놀 수지, 에폭시 수지, 접착제 등의 화학약품화 및 탄소섬유로 소재화하는 등의 리그닌 부가가치화 연구사례가 많아지고 있다. 본 연구 대상인 폐목재는 2015년 기준 연간 약 170만 톤이 발생하고 있으며 약 70%가 재활용되어지고 있으나, 3등급 폐목재의 경우 가공과정에서 페인트, 기름, 방부제, 접착제 등 다량의 불순물이 함유되어 재활용 시 많은 제약을 두고 있다. 이러한 폐목재의 특성을 고려한 재활용 방안에 대한 연구의 필요성이 존재한다. 본 연구는 3등급 폐목재를 대상으로 organosolv 전처리법을 이용한 최적 리그닌 분해 조건 도출 및 UF/NF 멤브레인 시스템 적용을 통한 폐목재 내 목적으로 하는 분자량을 가진 리그닌의 고순도 분리 추출을 목적으로 하고 있다. 또한 분리된 리그닌의 FT-IR, NMR, TGA 분석을 통해 폐목재 리그닌의 재활용 가능성을 검토해보고자 한다.

기존 골조구조물의 횡 변위 조절이 가능한 댐퍼 시스템을 개발하기 위한 목적으로 상세개발 및 성능실험을 실시하였다. 개발 상세는 기둥간 보의 변형을 방지하기 위하여 고안된 ALD및 층간변위를 제어하기 위하여 고안된 AWD로 구분되며, 기존 연구결과의 비보강 BF를 비교대상으로 사용하였다. 파괴양상, 하중-변위 곡선, 포락선, 최대강도, 강성저하 및 에너지 소산능력 등을 비교 평가하였으며, ALD 및 AWD 의 내진보강효과를 확인할 수 있었다. 또한 아라미드 시트로 기둥을 구속하는 공법이 내진성능 향상에 매우 우수함을 확인하였다.

Background : Oplopanax elatus, comnonly called Asian devil’s club, is distributed in the northern high mountains of the Korean peninsula, northeastern China, and southeastern Russia. Its root is traditionally used to treat asthma, depressive states, chronic fatigue syndrome, and hypertension, neurasthenia, hypopiesis, schizophrenia, cardiovascular problems, diabetes mellitus, impotence, emaciation, hypotension cough, rheumatism, arthritis, gastrointestinal disorders, and wounds. Plant cell and tissue culture techniques for the mass production of adventitious roots in suspension culture allows a high proliferation rate, stable accumulation rate, and production of valuable compounds. Methods and Results : The plants were cultured on 1/4 MS medium with 1% sucrose and 1% plant agar for 3 month and they transferred to pot. The pots were contained of soil, soil + pelite (1 : 1), and soil + sand (1 : 1), respectively. The plants were measured plant length, leaf length, and leaf number. The number of leaves have represented as similar result of 7.44 ± 1.34 ㎝ in soil and 7.56 ± 1.26 ㎝ on soil + pelite. The 3.22 ± 0.53 ㎝ of plant length and the 6.57 ± 0.51 ㎝ of leaves length were highest on soil. Conclusion : The survival rate depending on soil types of Oplopanax elatus was confirmed to exist in these cases of soil and soil+pelite. However, they all died in condition of soil+vermiculite. The number of leaves have no difference and the length of plant and the length of leaves were longest on soil.

Background : Anemarrhena asphodeloides has efficacy such as anti-fungal, anti-cancerous, anti-inflammatory, anti-diabetic, Anti-UV etc. Metal nanoparticles are used for photo imaging, cancer resection and drug delivery etc in medical field. Therefore A. asphodeloides nanoparticles will be expected better efficacy for therapeutic properties in medical field. Methods and Results : The water extract of A. asphodeloides mediated the synthesis of Aa-AgNPs and Aa-AuNPs. Their characterized by several physicochemical techniques such as UV-Vis spectrophotometry, FE-TEM, EDX spectroscopy, SAED pattern, DLS size analysis, XRD analysis, and FTIR analysis. Both Aa-Ag/AuNPs were evaluated for cytotoxicity towards 3T3-L1, A549, HT29 and MCF7. Aa-AgNPs and Aa-Au NPs were found to be spherical, face-centered cubic nanocrystals with hydrodynamic diameter of 190 and 258 ㎚. In vitro cytotoxic analysis revealed that up to 50 ㎍/㎖-1 concentration Aa-Au NPs did not exhibit any toxicity on 3T3-L1, HT29 and MCF7 cell lines, while being specifically cytotoxic to A549 cell line. On the contrary, Aa-Ag NPs displayed a significantly higher toxicity in all cell lines specially MCF7 cell line. ROS generation was not affected by Aa-Au NPs, but Aa-AgNPs has a higher potential to induce oxidative stress in A549 cells than HT29 and MCF7 cells. Aa-Au NPs have the potential for anticancer agent during lung cancer treatment. Aa-AgNPs is also exhibited to inhibit cell migration by induce oxidatie stress. Conclusion : The Aa-Au/AgNPs might have the anticancer potential and might be effective in the lung cancer therapy, however further evaluation is must needed.

가상현실은 현재 게임 산업에서 가장 주목하는 기술 중 하나다. 그럼에도 불구하고 가상현실 기술 자체가 극복해야 할 문제점과 시장가능성에 대한 의문으로 게임 산업에서 이에 대한 투자가 활발히 이루어지기 어려운 실정이다. 따라서 본 연구에서는 가상현실 게임 시장의 미래 가능성을 다각도로 타진해보고자 하였다. 이를 위하여 과거부터 현재까지 국내의 게임시장 발전 양상을 살펴보고, 시나리오네트워크매핑(SNM)을 활용하여 이해관계자 구조를 통한 미래예측을 시도하였다. 그 결과는 장·단기적 미래전망을 제시하고 이를 바탕으로 가상현실 게임 산업의 핵심이 될 이해관계자들에게 필요할 활동과 전략에 대한 시사점을 제시하였다는 데 공헌점이 있다.

The purpose of this paper is to assess the effect of surface characteristic of GFRP rebar on concrete-reinforcement bond behavior. For sample preparation, 10 GFRP rebar with two types of surface conditions were used to fabricate 15×15×15cm sized GFRP reinforced concrete. Then, pull-out test was carried out on each concrete samples until the maximum bond strength was achieved. From the test result, it was found that the maximum bond strength at the lattice type is 1.38 times higher than that for unidirectional surface.

The purpose of this paper is to clarify the boundary condition of the joint members in the multi-span greenhouse. It is carried out that experiments for the rotational stiffness of the joint members used in both the field and the standards of the greenhouse constructions.

This paper presents the basic data for the structural safety study of the extension wall by evaluating the bending stresses of the existing and extension walls varying according to the plan change of the extension building through the numerical analysis on the horizontal load as follows: 1) the stress variation of the divided wall when the wall opening due to a partial demolition is evaluated by comparing the result of analysis on whether or not the opening of the bearing wall is demolished as a variable; 2) the stress variation of the existing wall due to the increase of the stiffness of the extension wall is also evaluated by comparing the result of analysis on the thickness of the extension wall as a variable.

This paper analyzed design bearing capacities of pile foundations strengthened by pre-loading method for vertical expansion. The bearing capacities were investigated for two different pre-loading strategies: (1) after partial removals of structural/non-structural elements (e.g. finish, masonry wall etc.); and (2) after vertical expansions. Based on the numerical studies, more than 60% of the existing piles exceeded the design bearing capacities due to the difference of pre-loading strategies, which should be considered in structural design.

In this study, seismic strengthening method using precast concrete rocking wall is considered in the literature studies. As a result of reviewing the two studies, it is considered necessary to develop a sequential study on development of lightweight precast wall panel, application of post-tension method, and development of location and shape of energy dissipation devices.

In this study, the seismic retrofitting effect of existing frame was evaluated. The strength and energy dissipation capacities of three reinforcement methods were evaluated based on the existing literatures. In the case of strength and energy dissipation capacity, it was evaluated that reinforcing effect of specimen reinforced with damper by installing K brace inside the frame was excellent. Also, it was evaluated that there is an effect of seismic reinforcement in the external retrofitting case. Therefore, proper seismic retrofit planning is expected to ensure the seismic safety of buildings.