Background: The muscles associated with tension-type headache (TTH) are connected to the scalp fascia, which is firmly attached to the superciliary ridge of the frontal bone. However, there is a paucity of data on treatments for TTH that directly target the scalp fasciae.
Objectives: We aimed to validate a new manual therapy to treat TTH by applying myofascial relaxation techniques to the scalp and to examine the changes in quality of life and headache characteristics after treatment.
Design: pretest-posttest control group design study.
Methods: Total 24 participants with TTH (10 males, 14 females) participated in the study. Manual therapy group (MT) received standard manual therapy and scalp myofascial release technique group (SMT) received scalp myofascial release technique twice per week for 4 weeks. Brief Pain Inventory (BPI) and Headache Impact Test (HIT) for quality of life, headache frequency, duration, and Visual Analog Scale (VAS) for intensity were assessed at the pre- and post-treatment. Results: There was a significant improvement within groups in headache frequency (MT P<.05, SMT P<.001), duration (MT P<.05, SMT P<.01), VAS (MT P<.01, SMT P<.001), HIT (MT P<.001, SMT P<.001), and BPI (MT P<.001, SMT P<.001) between the pre- and post-treatment. Group differences were significant for headache duration (P=.027), but the others were not. MT and SMT reduced the impact of headaches on daily life. Also Headache frequency, duration, intensity, and BPI were improved.
Conclusion: MT and SMT reduced the impact of headaches on daily life. Also Headache frequency, duration, intensity, and BPI were improved. It has been suggested that both MT and SMT can be used as a non-invasive treatment to treat the TTH, and to improve the quality of life.
Background: The transcutaneous electrical nerve stimulation (TNES) is the most used non-invasive treatment method in physical therapy. As the mobile TENS (MTENS) has become popular, patients with pain have started using MTENS to reduce pain.
Objectives: To evaluate pain, range of motion, and muscle strength before and after using MTNES in patients with wrist pain.
Design: Quasi-experimental research.
Methods: We conducted an experiment with 80 patients; 15 patients were dropped out, and 35 and 30 patients were evaluated in the experimental group (EG) and control group (CG), respectively. Before and after using MTENS for 4 weeks, patients were evaluated using visual analogue scale (VAS), grip power, range of motion (ROM), and digital infrared thermography imaging (DITI). In the EG, electricity was applied for the MTENS device, while electricity was not applied in the CG. Results: A significant difference in pain reduction was observed between the EG and CG. In the EG, a significant difference in grip strength was also noticed before and after using the MTENS; patients showed significantly increased power grip and tip pinch. A significant difference was observed in pre-rest and post-test wrist ROM and DITI values.
Conclusion: MTENS is an appropriate procedure for patients with wrist pain.
목적: 초기노안을 대상으로 중심-근용 멀티포컬 콘택트렌즈 착용 시 구면수차가 시력에 미치는 영향을 확인하고자 하였다.
방법 : 초기노안 50명을 대상으로 중심-근용 멀티포컬 콘택트렌즈 착용하고 일주일의 적응 기간 후 원·근거 리 시력과 구면수차의 변화 및 원·근거리 시력 개선에 필요한 추가 교정도수를 확인하였고, 시력만족도는 설문조 사로 확인하였다.
결과 : 대상자의 평균 교정굴절력은 –2.65±1.67 D, 가입도는 +1.15±0.05 D이었으며, 안구 구면수차는 0.010±0.028 ㎛였으며, 이 중 37명(72 %)은 양의 구면수차(0.028±0.017 ㎛)를, 13명(28 %)은 음의 구면수차 (–0.020±0.010 ㎛)를 갖고 있었다. 중심-근용 멀티포컬 콘택트렌즈를 착용한 후 양의 구면수차를 가 진 그룹은 -0.012±0.005 ㎛로, 음의 구면수차를 가진 그룹은 –0.043±0.010 μm로 모두 음(-)의 방향으로 증 가하였다(p<0.050). 멀티포컬 콘택트렌즈를 착용한 후 100% 원거리 시력(logMAR)은 양의 구면수차를 가진 그룹 에서는 –0.014±0.090, 음의 구면수차를 가진 그룹에서는 0.022±0.120으로 양의 구면수차를 가진 그룹에서 더 좋았고(p<0.001), 100% 고대비 근시력(logMAR)은 양의 구면수차를 가진 그룹에서는 0.127±0.090, 음의 구면 수차를 가진 그룹에서는 0.118±0.090으로 음의 구면수차를 가진 그룹에서 더 좋았다(p<0.001). 설문조사 결과 에서는 양의 구면수차를 가진 그룹에서는 원거리에서 높은 만족도를 보였고, 음의 구면수차를 가진 그룹에서는 중 간거리와 근거리 영역에서 만족도가 높았다(p<0.050).
결론 : 중심-근용 멀티포컬 콘택트렌즈를 처방 후 구면수차가 변할 수 있고, 착용자의 안구 구면수차에 따라 원·근거리 시력 만족도가 달라질 수 있음을 확인하였다. 따라서 멀티포컬 콘택트렌즈를 처방할 때 착용자의 구면 수차를 확인한다면 처방 성공률을 더 높일 수 있을 것으로 사료된다.
목적 : 한국실명예방재단에서 발표한 사업보고서를 기초로 약시의 진단 및 원인, 치료 그리고 치료 결과에 대한 데이터를 분석하고자 한다.
방법 : 눈 건강증진 사업인 ‘취학 전 어린이 실명예방사업 보고서’ 중 2016년에서 2020년 최근 5년간의 보고서 내용 중 약시와 관련된 결과들을 분석하였다.
결과 : 약시 진단 및 원인으로 평균 50.87±3.97%로 절반 이상이 병원에서 약시를 확인하였으며, 평균 51.50±2.48%로 국내 약시안의 절반 이상이 굴절이상으로 인한 약시가 가장 큰 원인으로 나타났다. 약시 치료 기간은 1년 이하가 평균 54.42±9.27% 약시안의 절반 이상이 1년 이내에 치료가 완료된 것으로 나타났으며, 안 경 착용과 차폐를 병행한 방법이 평균 51.05±3.85%로 나타나 가장 많이 사용되고 있는 치료법이였다. 평균 68.86±13.13%가 시력이 향상되었으며, 치료 종결된 약시안은 31.98±23.53%를 보였다. 치료에 방해가 되는 원인에서는 아이의 치료거부가 42.34±3.76%로 가장 큰 이유로 나타났다.
결론 : 약시의 치료에 있어 가장 기본이 되는 것으로 시력이 가장 중요한 요소이며, 시력 향상을 유지하기 위해 서는 정확한 굴절검사를 바탕으로 한 안경교정 함께 정상적인 양안시를 유지하도록 하는 것이다. 국내 약시의 상 황을 정확하게 분석하기 위해서 본 연구를 기초로 앞으로 국내 약시의 지속적인 연구가 필요할 것으로 보인다.
목적 : 미세먼지가 젊은층과 노년층의 눈물막에 영향을 미치는지 알아보고자 미세먼지 농도 수준에 따라 눈물의 양, 안정성 및 지질층 두께 변화를 평가하였다.
방법 : 안질환이 없는 20대와 60대 각각 25명(총 50명)이 참석하였고, 한국환경공단에서 제공된 미세먼지 좋 음 수준(0~30 ㎍/m³)과 나쁨 수준(51~100 ㎍/m³)에서 각각 이들을 대상으로 눈물 검사를 하였다. 눈물양은 OCCUTUBE(OccuTech Co., Seongnam, Korea)로, 눈물막 안정성은 침습성눈물막파괴시간(TBUT), 비침습성 눈물막파괴시간(NIBUT)로 지질층 두께는 Lipiview® 로 평가하였으며, 건성안 관련 증상은 OSDI 설문지를 이용 하여 평가하였다. 미세먼지 수준에 따른 비교는 SPSS version 21.0(SPSS Inc, Chicago, IL, USA)를 사용하여 분석하였다.
결과 : 젊은층과 노년층 모두 미세먼지 나쁨 수준인 날에서는 눈물의 양과 안정성이 미세먼지 좋음 수준인 날과 비교하여 유의하게 감소하였으나(p<0.050), 지질층 두께와 OSDI 설문값은 미세먼지 수준에 따라 젊은층과 노년층에서 모두 유의한 차이가 없었다. 미세먼지 농도가 증가할수록 OCCUTUBE 검사값과 TBUT 와 NIBUT는 감소하는 상관성을 보였으며, 노년층의 상관계수(r=0.35, p<0.001, r=0.47, p<0.001, r=0.52, p<0.001, respectively)가 젊은층의 상관계수(r=0.34, p<0.001, r=0.37, p<0.001, r=0.40, p<0.001, respectively)보다 다소 높았다.
결론 : 미세먼지는 젊은층과 노년층에의 눈물의 양과 안정성을 감소시켜 건성안 유발요인으로 작용할 것으로 사료된다.
Enhancing income for small-scale farmers in developing countries, is one of the major concerns for governments and many stakeholders. This is attributed by the fact that, a majority of smallscale farmers in the region are characterized by low income earners caused by agronomic related challenges, such as low productivity of input factors. In addressing this challenge, the purpose of this study was to assess the impact of outgrower scheme on its members’ income, using the propensity score matching approach. Through this approach the study assessed cross-sectional data, collected from small-scale tea farmers in the Mufindi district, founded in Tanzania. Results of the assessment on the impact of outgrower scheme on its members, suggest that the scheme has a negative and insignificant impact on its members’ income. This implies that, the outgrower scheme does not meet its intended objective of raising the income of its members, due to failure of improving quality as well as quantity of tea produced in the study area.
This study investigated the influence of sodium bicarbonate (NaHCO3) and progesterone on acrosome reaction and proportion of polyunsaturated fatty acid (PUFA) composition boar sperm. The sperm were diluted with semen extender and incubated with NaHCO3 and progesterone at 38℃, 5% CO2 for 6 h. Plasma membrane integrity and acrosome reaction were analyzed using SYBR14/propidium iodide (PI) and FITC-PNA/PI doubling staining method, and proportion of PUFA was analyzed using gas chromatography. In results, Plasma membrane integrity was significantly decreased in 50 mM NaHCO3 group and acrosome reaction was significantly increased by over the 100 mM NaHCO3 group compared to control group (p < 0.05). In addition, progesterone significantly increased decreased plasma membrane integrity at 100 mM progesterone and acrosome reaction at over the 5.0 µM progesterone (p < 0.05), but there was no difference among the 5.0 to 100 µM groups. PUFAs were significantly decreased in 100 mM NaHCO3 and 50 µM progesterone treatments compared to control group. In summary NaHCO3 and progesterone induce acrosome reaction and reduce PUFA composition in boar sperm, therefore, the results maybe help to understand basically knowledge for the acrosome reaction and PUFA composition in boar sperm.
Fescues, which are widely cultivated as grasses and forages around the world, are often naturally infected with the endophyte, Epichloë. This fungus, transmitted through seeds, imparts resistance to drying and herbivorous insects in its host without causing any external damage, thereby contributing to the adaptation of the host to the environment and maintaining a symbiosis. However, some endophytes, such as E. coenophialum synthesize ergovaline or lolitrem B, which accumulate in the plant and impart anti-mammalian properties. For example, when livestock consume excessive amounts of grass containing toxic endophytes, problems associated with neuromuscular abnormalities, such as convulsions, paralysis, high fever, decreased milk production, reproductive disorders, and even death, can occur. Therefore, pre-inoculation with non-toxic endogenous fungi or management with endophyte-free grass is important in preventing damage to livestock and producing high-quality forage. To date, the diagnosis of endophytes has been mainly performed by observation under a microscope following staining, or by performing an immune blot assay using a monoclonal antibody. Recently, the polymerase chain reaction (PCR)-based molecular diagnostic method is gaining importance in the fields of agriculture, livestock, and healthcare given the method’s advantages. These include faster results, with greater accuracy and sensitivity than those obtained using conventional diagnostic methods. For the diagnosis of endophytes, the nested PCR method is the only available option developed; however, it is limited by the fact that the level of toxic alkaloid synthesis cannot be estimated. Therefore, in this study, we aimed to develop a triplex real-time PCR diagnostic method that can determine the presence or absence of endophyte infection using DNA extracted from seeds within 1 h, while simultaneously detecting easD and LtmC genes, which are related to toxic alkaloid synthesis. This new method was then also applied to real field samples.
This study was conducted to investigate the forage characteristics of very early-maturing Italian ryegrass (IRG) three cultivars within September, 2021 to July, 2022 in Cheonan, Korea. We used three different cultivars of Italian ryegrass in this study “Greenfarm” “Greenfarm2ho” and “Greenfarm3ho” The results of heading date in this study, “Greenfarm” was recorded 3 days earlier than the other two cultivars, and its lodge tolerance score was the highest among the three. However, in case of plant length, there was no significantly difference in whole cultivars, in addition disease resistance, insect resistance and cold tolerance were not significantly different in three cultivars. The forage productivity of “Greenfarm2ho” was recorded the highest, especially the 1st harvesting of “Greenfarm2ho” yielded significantly the highest and superior to other varieties. As a result of feed value analysis, three cultivars were generally superior to overseas varieties and in particular, “Greenfarm2ho” was recorded to have excellent value because of the lowest NDF and ADF content. On the other hand, there was no significant difference in crude protein content among three varieties. In case of the monosaccharides content "Greenfarm3ho” had significantly higher glucose content than other two varieties, therefore the “Greenfarm” has advantage for preparing high quality of silage. In contrast, there was no significant difference among three varieties in fructose content.
Avian colibacillosis is severe respiratory and systemic disease caused by avian pathogenic E. coli (APEC). The disease results in economic loss because of early mortality and reduced productivity in chickens. Several antimicrobials have been used extensively for reducing the losses due to colibacillosis. β-lactams, including penicillin derivatives, cephalosporins, and carbapenems, are one of the most commonly used, clinically important antibiotics. In this study, the phenotypic and genotypic characteristics of 14 CTX-M-producing APEC were investigated. Two types of CTX-M genes were detected, namely, CTX-M-1 gene (8 isolates) and CTX-M-15 gene (6 isolates), and all CTX-M-producing APEC were multidrug resistant. Moreover, 10 of the 14 CTX-M-producing E. coli isolates carried the TEM-1 gene, and 8 of the 14 isolates contained class 1 integrons. The following three different gene cassette arrangements were identified in six isolates: aadA1 (n=2), dfrA1 (n=2), and aadA1+dfrA1 (n=2). All CTX-M-producing APEC isolates harbored IncF plasmids, including F, FII, FIA, and FIB. CTX-M-1 gene was located on I1, F, and FIB, and CTX-M-15 gene on FII, FIA, and FIB. The findings from this study indicate that E. coli carrying CTX-M gene are circulating in the broiler poultry industry. Comprehensive surveillance and persistent monitoring of the integrated broiler operations are warranted to prevent the dissemination of resistant isolates.
This study evaluated the effect of a combination of acetaminophen (AAP), vitamin C (VC) and thioctic acid (TA) on reducing serum cortisol concentrations in mice with foot-and-mouth disease (FMD) vaccination. For 5 days from 3 days before FMD vaccination to 2 days after vaccination, mice were orally administered with AAP 600 mg/kg feed (n=25, AAPT), combination of AAP 600 mg and VC 200 mg per kg feed (n=25, AVCT) and combination of AAP 600 mg, VC 200 mg and TA 20 mg per kg feed (n=25, AVTT), respectively. From day 1 to day 7 after FMD vaccination, the cortisol concentration of all groups treated with the drug was significantly decreased compared to that of the positive control group with FMD vaccination (p<0.05). In conclusion, the combined treatment of AAP, VC and TA was the most effective in relieving stress from FMD vaccination compared to the single treatment.
Bovine mammary epithelial (MAC-T) cells are commonly used to study mammary gland development and mastitis. Lipopolysaccharide is a major bacterial cell membrane component that can induce inflammation. Autophagy is an important regulatory mechanism participating in the elimination of invading pathogens. In this study, we evaluated the mechanism underlying bacterial mastitis and mammary cell death following lipopolysaccharide treatment. After 24 h of 50 μg/mL lipopolysaccharide treatment, a significant decrease in the proliferation rate of MAC-T cells was observed. However, no changes were observed upon treatment of MAC-T cells with 10 μg/mL of lipopolysaccharide for up to 48 h. Thus, upon lipopolysaccharide treatment, MAC-T cells exhibit dose-dependent effects of growth inhibition at 10 μg/mL and death at 50 μg/mL. Treatment of MAC-T cells with 50 μg/mL lipopolysaccharide also induced the expression of autophagy-related genes ATG3, ATG5, ATG10, ATG12, MAP1LC3B, GABARAP-L2, and BECN1. The autophagy-related LC3A/B protein was also expressed in a dose-dependent manner upon lipopolysaccharide treatment. Based on these results, we suggest that a high dose of bacterial infection induces mammary epithelial cell death related to autophagy signals.
This study was carried out to investigate the effects of planting densities on the growth characteristics, dry matter yield, and feed value of “Geukdong 6” (a new variety of corn for feed). The experimental design was arranged in a randomized block design with three replications. Treatments consisted of six planting densities, 60 cm × 25 cm (T1), 60 cm × 30 cm (T2), 70 cm × 25 cm (T3), 70 cm × 30 cm (T4), 80 cm × 25 cm (T5) and 80 cm × 30 cm (T6). All treatments were sown on May 14, 2021, and the harvest was on October 3 (late flowering). Plant length and the number of tillers were the highest in T5 (p<0.05), but the number of leaves and stem diameter were the highest in T6 than in the other treatments (p<0.05). Leaf length, leaf width, and dead leaf were not significantly different among the treatments. Organic matter was highest in T6, and crude protein was highest in T5 (p<0.05). The ether extract was not significantly different among the treatments. Crude fiber, NDF, and ADF were highest in T2 with relatively higher planting density (p<0.05). Calcium and phosphorus were not significantly different among the treatments. TDN content was the highest in T3 (p<0.05). Sugar degree (Brix), fructose, glucose, dextran, isomerose, and inverted sugar were not significantly different among the treatment. Fresh yield, dry matter yield and TDN yield were higher in order of T6 > T5 > T4 > T3 > T2 > T1 (p<0.05). Relatively feed value was higher in order of T3 > T6 > T5 > T1 > T4 > T2 (p<0.05). Based on the above results, planting density could be recommended from 80 cm × 30 cm for efficient production of “Geukdong 6”.
Aster 속에 속하는 자생식물로는 눈갯쑥부쟁이, 개쑥부쟁이, 쑥부쟁이, 벌개미취, 참취 등이 있는데, 대부분 식용 또는 관상용 으로 많이 활용되고 있다. 특히 눈갯쑥부쟁이는 제주 한라산(표 고 1,200~1500m근처)에서 자생하는 한반도 특산식물로써, 다른 Aster 속과 다르게 포복성을 가지고 있고 개체당 소화의 수가 많아 관상가치가 매우 뛰어나다. 또한 파종 당해년에도 개화가 가능하기 때문에 유망한 관상식물 자원이라 할 수 있다. 본 연구 에서는, 한반도 특산 눈갯쑥부쟁이의 유전자원 보존 및 대량번 식을 위한 기초자료를 확보하고자 종자의 발아특성 및 휴면유형 을 분류하였다. 눈갯쑥부쟁이 종자는 형태적으로 완전하게 발달 된 배를 가지고 있고, 배양 72시간 내에 145%의 수분을 흡수하 였다. 4가지 온도조건(4°C, 15/6°C, 20/10°C, 25/15°C)에 배양한 결과, 광조건에서는 각각 67.0%, 58.9%, 62.2%, 71.6% 발아하였 고, 암조건에서는 각각 79.4%, 65.9%, 65.9%, 49.1% 발아하였 다. 저온층적처리(4°C) 실험 결과, 최종 발아율에는 큰 차이가 없었으나, 층적처리 기간 동안에도 발아하는 특성을 관찰하였 다. GA3처리 후 25/15°C에 배양한 결과, 0, 10, 100, 1000mg·L-1 처리에서 각각 57.9%, 68.3%, 74.9%, 63.9%의 발아율을 보였 다. 15/6°C에 배양한 경우에는 각각 78.3%, 62.8%, 72.2%, 55.9% 발아하였다. 본 연구에서는 위 실험들을 통하여, 약 80% 정도는 non-dormant 종자로, 나머지 약 20%는 생리적 휴면 (physiological dormancy)을 가지고 있는 것으로 판단되었다.
한라개승마(Aruncus aethusifolius (H.Lév.) Nakai)는 관상 용 및 약용 식물로 이용이 될 가능성이 있는 한국 고유종이 다. 그러나 종자에 의한 번식에 대해서는 알려진 바가 거의 없다. 이 연구는 한라개승마의 종자번식 프로토콜을 개발하기 위해 수행되었다. 실험에 사용된 종자는 2019년 11월 3일 한 택식물원에서 채종하였으며, 안동대학교 유전자원포지에서 관 리하던 식물체에서 2020년 11월 10일에 채종하였다. 한라개 승마 종자는 완전히 성숙한 배를 가지고 있었다. 종자는 모식 물체에서 탈리되고 이듬해 3월부터 발아하기 시작하였다. 수 분흡수실험 3시간 이내에 초기 건조상태의 무게 대비 25% 이 상 무게가 증가하여 물리적휴면이 없는 것을 확인하였다. 명 암 조건에서의 온도별 발아시험 결과, 한라개승마 종자의 발 아율은 암조건보다 명조건에서 높았고, 명암 조건 모두 4°C에 서 발아하지 않았다. 따라서 종자는 광발아종자이므로 soil seed bank를 형성할 가능성이 있다. GA 실험의 경우 2019년 수확한 종자와 2020년 수확한 종자의 효과가 다르게 나타났 다. 저온습윤처리 기간이 길어질수록 발아소요일이 앞당겨지 는 것으로 나타났다. 그러나 2020년도 종자는 발아율이 낮아 서 12주간의 저온습윤처리 이후에도 발아율이 40%를 넘지 못 했다. 그리고 후숙처리 기간은 한라개승마 종자의 발아에 영 향을 미쳤다. 실험결과 2019년 수확한 종자와 2020년 수확한 종자의 발아율 차이가 확인되었다. 이는 모식물체의 생육 환 경에 따른 영향으로 판단된다.
Aluminum (Al) is one of the major factors adversely affects crop growth and productivity in acidic soils. In this study, the effect of Al on plants in soil was investigated by comparing the protein expression profiles of alfalfa roots exposed to Al stress treatment. Two-week-old alfalfa seedlings were exposed to Al stress treatment at pH 4.0. Total protein was extracted from alfalfa root tissue and analyzed by two-dimensional gel electrophoresis combined with MALDI-TOF/TOF mass spectrometry. A total of 45 proteins differentially expressed in Al stress-treated alfalfa root tissues were identified, of which 28 were up-regulated and 17 were down-regulated. Of the differentially expressed proteins, 7 representative proteins were further confirmed for transcript accumulation by RT-PCR analysis. The identified proteins were involved in several functional categories including disease/defense (24%), energy (22%), protein destination (9%), metabolism (7%), transcription (5%), secondary metabolism (4%), and ambiguous classification (29%). The identification of key candidate genes induced by Al in alfalfa roots will be useful to elucidate the molecular mechanisms of Al stress tolerance in alfalfa plants.