자생 상록양치류의 대량번식의 체계를 위해 배양조건에 따른 전엽체 유도와 발달에 미치는 배지, 질소급원, 탄소원, 생장조절물질의 조합 및 배양조건를 조사한 결과는 다음과 같다. 전엽체증식에 있어 적절한 배지는 4종 모두 MS배지가 전엽체의 증식에 가장 양호하였고, 질소량이 NH4+:NO3-이 20:40mM로 첨가된 배지에서 가장 양호하였는데, 특히 큰봉의꼬리(Pteris cretica)에서는 NH4+:NO3-이 60:0보다 20:40mM에서 생체중이 1.48g으로 4.1배로 증가를 보여 가장 양호하였다. 탄소원인 sucrose 농도별로 전엽체의 증식에 큰 차이를 보였는데, 큰봉의꼬리(Pteris cretica)에 있어 3% 첨가구에서 대조구에 비해 7.7배로 전엽체의 증식이 가장 양호하였고, 배지내의 NaH2PO4 100mg/L에서 더부살이고사리(Polystichum lepidocaulon)의 경우에 있어 가장 양호하였으며 전반적으로 유사한 경향을 보였다. 생장조절 물질에 대한 생육반응에 있어 골고사리와 버들참빗은 NAA 0.1mg/L에서 볼 수 있었으나, 돌담고사리 경우에는 Kinetin 0.01∼10mg/L와 NAA 0.01∼1mg/L에서는 효과의 차이를 볼 수 없었다. 손고비에서는 전반적으로 무처리구에서 전엽체 증식이 가장 현저하였다. 배양시 광조건은 1,000 Lux의 광도에서 배양한 것이 전엽체 증식률이 가장 높았으며, 배양방법에 있어 큰봉의꼬리(Pteris cretica)는 액체배양에서는 1.59g에 비해 고체배양에서 3.34g으로 2.1배의 현저한 증가를 볼 수 있었다.

The volatile aromatic substances from Cinnamomum camphora and C. japonicum among warm-temperate tree species were examined for the physiological and psychological effects on the human body. Each scent of the trees was provided to the test materials. And then their brain wave, pulse rate, aroma sensibility evaluation and POMS were measured as follows. To examine psychological effect of aroma of each tree species on human bodies, aroma sensibility evaluation and POMS were conducted. The result reveals that the aroma of the two tree species were interpreted in terms of comfort, naturalness and stimuli, and that C. camphora shows low level of satisfaction due to strong aroma while C. japonicum is more preferred due to its soft and fragrant aroma. As well, POMS was given to identify subjects' temporary emotional state. The result indicates that C. camphora has higher values in every item than C. japonicum, and that both tree species repress anxiety and depression and create vivacious feelings with higher values in [vivacity] and [anger-hostility] items than [tense-anxiety] and [distress-depression]. To examine the physiological effect of aroma of each tree species, aroma extracted from the two tree species were distributed, and the subjects' brain wave and impulse were measured. The result demonstrates that C. camphor has relaxing effect of increasing α-wave and reducing pulse, and that C. japonicum also has relaxing effect, but the effect is less than that of C. camphora as it reduces pulse though it slightly reduces α-wave. In addition, individual preferences for C. camphora vary as it has big individual deviations vary, but C. japonicum has similar level of preference as its variances are small. The natural and pleasant aroma of these two tree species seem to have tranquilizing effect in both the physiological and psychological aspects and are found to have positive effects of feeling refreshed, feeling enhanced, and so on. In conclusion, there is a sufficient possibility of developing these two aromatic warm-temperate tree species. Also, the importance of such difference between these two tree species lies in the fact that they can set standards for the utilization of warm-temperate forest in the future.