한국산 반날개속에 대한 분류학적 재검토를 수행하였다. 그 결과, 4종이 한국산 미기록종으로 밝혀졌다(p. aeneipennis Boheman, P. daimio Sharp, P. nakanei Sawada, and P. numata Dvo k). 미기록종에 대한 종 기재와 수컷 생식기 구조를 도식하였다. 한국산 반날개속은 총 26종이 된다.

This study was carried out to isolate and identify Salmonella species from poultry slaughterhouses and pork meat processing plants during the period from January 1997 to August 1998, and analyze resistance of antimicrobial agents and plasmid profiles of isolated Salmonella strains. A total of 15 Salmonella strains was isolated from poultry carcasses, swine carcasses, pork meats and cutting boards. Identified Salmonella strains were S. typhimurium, S. heidelberg, S. hilingdon, S. mbandaka, and S. virginia. Ten (66.7%) of 15 Salmonella strains showed resistance to antimicrobial agents and five strains (33.3%) of them were resistant to two or more antimicrobial agents. Plasmids were isolated from three Salmonella isolates which had two or more plasmids.

국내 관련 연구소 및 대학 등에 소장된 표본과 일부 북한산 표본 등을 대상으로 분류동정한 결과 총 17종이 확인되었다. 이 중 Bombus (Tricornibombus) atripes는 한반도에서 처음 기록하며 B. (Thoracobombus) humilis subbaicalensis Vogt는 남한에서 처음으로 기록한다. 이들을 포함한 한반도산 7 아속 21종에 대한 검색표와 채집기록, 각 종의 기주 개화식물에 대하여 기술하였다.

한국산 빗살방아벌레아과(Melanotinae)의 표본을 동정하고 기록 문헌을 정리한 결과 총 18종이 확인되었다. 그 중 Melanotus속으로는 M.chejuensis sp. nov.와 M. coreanus sp. nov.을 포함하여 11종이 확인되었다. 신종에 대하여 기재하고 각 종들의 성충 및 수컷생식기 사진과 함께 각 속, 종들에 대한 검안표를 작성하여 보고한다.

한국산 왕거미과(Araneidae) 꽃왕거미속의 1미기록종 Araniella yaginumai Tanikawa, 1995 꽃왕거미(신칭)을 강원도 철원군 비무장지대에서 채집하였기에 이에 기재 발표한다.

An enzyme-linked immunosorbent assay (ELISA) was established for the detection of zearalenone by using monoclonal antibodies produced by Z-M-26 hybridoma cells when injected into a mouse and zearalenone-oxime-OVA conjugate. Zearalenone-oxime-OVA conjugates were diluted with carbonyl buffer, coated to 96 well microtiter plates at 4℃ overnight and blocked with 1% BSA overnight. One thousand times diluted antibody solution together with standard zearalenone or sample was added to 96-well microtiter plates and stood overnight. A secondary antibody conjugated with HRP was added and an hour later, enzyme substrate (TMBZ) solution was added for color develpment. After 30 minutes, coloring reaction was terminated by adding 2 N H₂SO₄ and the O.D. was measured at 450 nm. Detection range of this method was about 0.1-100 ppb. The established indirect competitive ELISA method was suitable for a rapid and effective analysis of zearalenone in agricultural products.

It was performed to investigate for Yersinia species from 2,841 spring waters in Seoul, from 1994 to 1998. Of them, Yersinia spp. were isolated 86 isolates (3.3%). Of 86, sixty two isolates (72.1%) were Yersinia enterocolitica, followed by Y. aldouae (11 strains), Y. pseudotuberculosis (5 strains), Y. frederiksenii (3 strains), unclassified Yersinia spp. (5 strains). Yersinia spp. were highest isolated from Nowon-Gu (22 samples) and Bukhan Mountain Park isolates (18 samples). We tested 1.186 samples for SPC and coliform from 1996 to 1998. Of these tests, the positive rate of coliform was 23.6%, SPC, 9.1%, and either coliform or SPC positive 27.1%. The positive rates of coliform and SPC were decreased 26.7%, 12.7% in 1996, 25.8%, 6.3% in 1997 and 18.1%, 7.6% in 1998, respectively. Of Y. enterocolitica, 78% was resistant to ampicillin and carbenicillin. In the case of Y. aldouae, only 3 of 11 isolated were resistent to carbenicillin. Y. pseudotuberculosis were resistant to colistin. Also Y. frederiksenii to carbenicillin. There were many spring waters of Y. enterocolitica isolated from Nowon-Gu and Buk-han Mountain Park. So, it needs to clean the environment of those regions.

농산물의 갈무리시 발생되는 건조한 마늘, 쪽파 및 대파 뿌리 분말을 흡착제로 이용하여 수용액 중 이온 상태로 존재하는 Ni과 Pb의 흡착력을 시료의 입자별, 중금속의 농도별, 용액의 온도별, pH별로 분석하였다. 중금속 이온들은 흡착제의 입자크기가 작을수록 흡착률이 높았고, 그중 마늘뿌리가 Pb에 대해 높은 흡착력을 나타냈다. 수용액중 중금속 농도가 높을수록 중금속들이 흡착되는 양이 증가하였고, 흡착률은 흡착제와 중금속의 종류에 따라 차이가 있었다. 온도의 증가는 쪽파와 대파에서 Ni과 Pb가 감소되었다. 알칼리 조건에서 비교적 흡착이 잘 되는 중금속은 Ni이며, 중성과 산성조건에서는 Pb의 흡착량이 높았다.

In order to investigate the species, serobiological characteristics and antibiotic sensitivity of Pseudomonas spp, we isolated Pseudomonas spp from 57 spring waters around Seoul area for spring, summer and autumn and identified Pseudomonas spp by biochemical characteristics and serological method. And also we tested the antibiotic sensitivity test by discdiffusion method. Of 57 spring waters tested, Pseudomonas spp were isolated from 33 spring waters(57.9%). Isolation rate of Pseudomonas spp in spring season was 28.1%, summer 21.1% and autumn 28.1%. Only 1 spring water was detected Pseudomonas spp in all seasons and 9 (15. 8%) were detected for 2 seasons and 13 (22.8%) were for only 1 season. Isolation rate of Pseudomonas spp at Mt. Cheonggye was 50% and followed by Mt. Bookhan 35.7%, Mt. Daemo 33.3%, Mt. Dobong 29.6%, Mt. Surak 25.9%, Mt. Woomyun 22.2% and Mt. Bulam 7.4%. Of 44 Pseudomonas spp, 22 strains (50%) were identified by Ps. putida, Ps. aeruginosa, Ps. tluorescens and Ps. mendocina were identified 6 strains (13.6%), respectively. 4 strains (9.1%) were identified by Ps. aureofaciens. Of 6 Ps. aieruginosa, serotype A was 2 strains, B, E, G, and K was 1 strain, respectively. Of 44 Pseudomonas spp, resistance rate to amoxicillin was 90.9% and followed by chloramphenicol 84.1%, tetracycline 84.1%, carbenicillin 81.8%, nalidixic acid 68.2%, neomycin 38.6%, streptomycin 31.8%, gentamicin 4.6%, kanamycin 4.6% and colistin 2.3%. Ps. aseruginosa was more sensitive to carbenicillin than other Pseudomonas spp isolated from spring waters in Seoul area but more resistant to kanamycin, and Ps. aureofaciens was no resistant to streptomycin. Among multiple drug resistance, resistance to 5 drugs was 31.8%, 4 drugs 15.9%, 7 drugs 13.6%, 1 drug and 2 drugs 4.6%, and 8 drugs 2.3%, respectively. The multiple resistance patterns detected highestly were NA-CB-C-TE-AMC (18.2%), NA-CB-N-C-TE-AMC (13.6%), CB-C-TE-AMC (11.4%) and NA-CB-N-C-TE-AMC-S (9.1%).

방아벌레과 빗살방아벌레속(Melanotus Eschscholtz)에 속하는 4신종에 대하여 형태적 기술, 그림 및 사진과 함께 보고한다.

Scaeosopha속의 1종을 신종으로 기재, 발표한다. 이 속은 구북구지역에서 처음으로 기록된다. Scaeothyris Diakonoff은 Scaeosopha Meyrick의 synonym으로 정리한다.

한국산 알모양우단풍뎅이속(검정풍뎅이과, 우단풍뎅이아과)에 대하여 분류학적인 검토를 실시하였다. 지금까지 한국산으로 보고된 23종 중 8종은 오동정에 의하여 기록된 것으로서 실제적인 한국산은 15종뿐이나 이중에서도 2종은 한국분포가 의심된다. 확정된 한국산중 다음과 같은 9종은 3개의 complex로 간주한다. M. holosericaea complex : M. holosericea, M. Schoenfeldti, M. renardi M. orientalis complex : M. cariniceps, M. orientalis, M. fusania M. verticalis complex : M. castanea, M. verticalis, M. ovatula