주상복합건물의 강체거동을 보이는 연결층은 인접한 층에 비해 큰 질량과 강성차에 의해서 수치해석상의 문제점을 일으킨다. 강체거동을 보이는 구조물의 정적해석은 상부와 하부구조물을 별개의 구조물로 보고 2단계에 걸쳐서 근사적으로 수행될수 있지만, 동적해석은 구조물을 분리해서 행해질 수 없다. 이러한 주상복합건물의 동적해석을 위한 효율적인 모형화기법이 본 연구에서 제시된다. 자유도수를 줄여서 컴퓨터해석시간을 대폭 감소시키는 행렬의 응축기법과 연결층을 모형화하기 위해서 일반적인 보요소에 강체가 연결된 요소를 도입한다. 제안된 모형화기법의 효율성과 정확성을 입증하기 위해서 몇가지 구조해석을 한다.
To improve the efficiency of in vitro production of embryos with follicular oocytes in Korean Native cows, the recovery rates, in vitro maturation, fertilization and development, and the time required for collecting and processing oocytes by aspiration with or without slicing were evaluated comparatively. The ovaries were obtained from a local abattoir and placed in physiological saline at 25~28 and brought to the laboratory within 3 hrs. The oocytes were collected by aspiration of follicles(2~6mm) with or without slicing ovaries after aspiration, and classified into Grade I, Grade II, Denuded, Expanded oocytes by the morphology of cumulus cells attached and the homogeneity of cytoplasmic granules. Also the time required for each step of collecting and processing oocytes were measured. The cumulus cells were removed in some Grade I oocytes to measure their size and nuclear configuration before and after in vitro maturation. The Grade I oocytes were matured in vitro(IVM) for 24 hrs. in TGM-199 supplemented with 35g /ml FSH, 10g /ml LH, 1 g /ml at 39 under 5% C02 in air. They were fertilized in vitro(IVF) by epididymal spermatozoa treated with heparin for 24hrs. and then the zygotes were cocultured in vitro (IVC) with bovine oviductal epithelial cells for 10 days. The results obtained were as follows: The number of oocytes recovered per ovary was averaged 6.6 by aspiration and 11.2 by slicing post aspiration, which summed to 17.8. The number of Grade I oocytes recovered per ovary was averaged 3.1 by aspiration and 3.6 by slicing, which summed to 6.7. The percentage of Grade I to total oocytes recovered was significantly(P<0.05) higher as 48.0 % in aspiration than 31.6% in slicing post aspiration. The time requlred for recovering a Grade I oocyte by aspiration and slicing was 1.1 and 2.5 min, respectively. The mean diameter of Grade I oocytes by aspiration and slicing was similar as 148.7 and 151.5m, respectively. The percentage of Metaphase II stage oocytes after IVM for 24 hours was significantly (P
This study was conducted to improve the production efficiency of in vitro produced (IVP) embryos in Korean Native cows. The optimal conditions and procedures for in vitro maturation(IVM), in vitro fertilization(IVF) and in vitro culture(IVC) of bovine follicular oocytes and IVP embryos were evaluated. Immature follicular oocytes were collected fiom the follicles of bovine ovaries obtained from abattoirs. The oocytes of Grade I and II for IVM were cocultured with monolayered bovine oviductal epithelial cells(BOEG) or granulosa cells in TCM-199 solution supplemented with follicle stimulating hormone, lutenizing hormone, estradiol-17 and heat inactivated fetal calf serum at 39 under 5% in air for 14 to 24 hours. Most of the oocytes(93%) matured to metaphase II in 24 hours. The cocultured IVM oocytes were fertilized in vitro at significantly(P<0.05) higher rate with BOEC(83.8%) and with granulosa cells(84.6%) than the non-cocultured IVM oocytes(73.6%). The IVM-IVF embryos developed to morula and blastocyst at significantly(P<0.05) higher rate in coculture with BOEC(41.2%) than with granulosa cells(23.1%) or conditioned medium(23.4%).
The combined hydroacoustic and bottom trawl surveys were conducted in the Cheju southeastern area by the training ship "KAYA" belong to Pukyong National University in July 1995 and the traning ship "NAGASAKI MARl]" belong to Nagasaki University in April 1994, respectively. The main purpose of the investigations was to provide the basic data for the management and the biomass estimation of commercially important demersal fish stocks in this area. Fish samples were collected by bottom trawling from 10 trawl stations randomly selected in the survey area, and the species and length compositions of trawl catches were examined. The fish school target strength for demersal fish aggregations was related to the catchability of trawl net with a 90 mm mesh codend. The most abundant species in the 1995 trawl stations were Japanese flying squid, sword tip squid and red horsehead and that of the 1994 trawl stations Japanese flying squid and blackmouth goosefish. The average weight per cubic meter of trawl catches collected by bottom trawling in the Cheju southeastern area were 1.0791×lO-4kg/m3 in the 1994 survey area and 1.3636×lO-4kg/m3 in the 1995 survey area, respectively. The catch data by cover net suggest that the efficiency of trawl net could affect the weight normalized target strength values for demersal fish aggregations. That is, the average target strength per unit of weight dropped from - 33.1 dB/kg using the total catch by codend and cover net to - 30.5 dB/kg using only the catch data by codend, and a change of2.6 dB/kg was observed.ange of2.6 dB/kg was observed.
This study was carried out to obtain good livestock production in comparison with 3 different pasture mixture (Control : TI = Potomac orchardgrass + Bastion perennial ryegrass + Fawn tall fescue + Regal white clover, T2 = Potomac orchardgrass + Bastion pe
RAPD-PCR(Random Amplified Polymorphic DNAs-Polymerase Chain Reation) 기법에 누에의 유전적 변이 분석을 위한 첫 단계로 다양한 GC함량을 갖는 random primer에 의해서 증폭되는 DNA 단편의 양상 및 증폭도를 비교하였다. RAPD-PCR을 위한 random primer의 증폭도는 GC함량에 의해서 상당히 영향을 받음이 분석되었다. 특히, 50% GC 함량을 갖는 primer는 그 증폭도에 따라서 4가지의 그룹으로 DNA단편이 증폭되었으며 〔bad amplification (75.5%), poor amplification (11.1%), good and excellent amplification(11.1%)〕, primer의 GC 함량이 증가할수록, 휠씬 더 좋은 증폭도를 보여주었다. 그러나, 40% GC 함량을 갖는 primer에 의해서는 어떤 증폭산물도 관찰되지 않았다. PCR을 수행하기 전에 6가지의 제한효소(BamHI, HindIII, Xbal, HaeIII, MspI, Rsal)를 사용하여 누에 genomic DNA를 처리하여 이를 주형 DNA로 하여 RAPD-PCR을 수행한 결과, 유전적 마커의 생산에 대한 효율이 증가함을 알 수 있었다. 이상의 결과를 종합해 볼 때 60%이상의 GC함량을 갖는 random primer와 전처리한 주형 DNA의 사용은 여러 가지 다른 누에 계통의 동정 및 연관군 지도작성에 따른 경비 및 시간을 줄이는데 효율적이라고 사료된다.
The purposes of this study were to: (a) identify college students' patronage behaviors, (b) develop an instrument measuring the attitudes of University Students towards university foodservices management practices, (c) determine university students' attitude towards the four types of university foodservices, and (d) provide recommendations on marketing strategies for university foodservice. Questionnaires were hand delivered to 600 Yonsei University students by designated coordinators. A total of 549 questionnaires were usable; resulting in an 93.3% response rate. The survey was conducted between November 28 to December 4, 1995. Statistical data analysis was completed using the SAS Programs for descriptive analysis, T-test, χ2 test, ANOVA, Factor Analysis and Stepwise Multiple Regression. Most (88.3%) of students were patronizing university foodservices for lunch. Underground student foodservice (40.1%) and Restaurants outside the campus (33.7%) were primarily used for lunch and dinner respectively. Eighty six percent of university students had 1 to 2 meals per day at university foodservices. The reasons given by students for patronizing university foodservices were as follows: location, time, price, menu, taste. Most of the respondents were least satisfied with hygiene, taste, menu and atmosphere. Data indicated strong support for eight priori dimensions in terms of food, menu, atmosphere, hygiene, employee attitude, facilities and convenience. After the factor analysis, price, fast service and foodservice location attributes were rearranged, combined and created a new dimension called as 'access'. Three dimensions in terms of menu, hygiene, convenience were important to students although performance was perceived as poor through importance-performance analysis. Most of students were not satisfied with all four types of university foodservices. In terms of food quality and price which university foodservices offer, most of respondents were moderately satisfied. According to multiple regression analysis, 93.31% of the variance respondents' satisfaction score could be explained by food, menu, price, atmosphere, hygiene, employee attitude, facilities, and convenience dimensions.
본 연구는 한국 제조업의 기업별 자료를 이용하여 확률적 생산함수를 추정한 다음, 우리나라의 주종 수출산업인 섬유산업과 전자산업의 기술적 효율성이 1980년대에 하락한 이유를 규명하기 위하여 동쪽산업내의 각 기업들의 기술적 효율성의 결정요인을 계량분석을 통해 분석하였다. 성장산업인 전자산업과 사양산업인 섬유산업의 산업내 (intra-industry) 기술적 효율성의 결정요인은 相異하였다. 성장산업인 전자산업의 경우, 상대적인 기업의 크기와 노동비용의 상대적 크기는 기술적 효율성과 각각 正의 관계를 보이고 있는 데 비해, 사양산업인 섬유산업의 경우, 총 매출액에서 수출이 차지하는 비중과 기술적 호율성은 正의 관계를 보인 데 反해, 총 생산비에서 노동비용이 차지하는 비중은 기술적 효율성과 負의 관계를 보였다. 즉 1980년대 섬유산업의 기술적 효율성이 하락한 중요한 요인은 동 산업의 수출부진과 상대적인 노동비용의 상승을 들 수 있으며, 성장산업인 전자산업의 경우는 일부 대기업의 집중도 심화가 동 산업의 평균적 기술적 효율성의 하락요인이 되었음을 알 수 있다. 따라서 본 연구는 경쟁의 촉진과 기업 집중도의 완화가 기술적 효율성을 향상시키는 데 중요하다는 점을 보여주고 있다.